1.
Rev Cubana Med Trop
; 49(2): 100-7, 1997.
Artigo
em Espanhol
| MEDLINE
| ID: mdl-9685971
RESUMO
The immunohistochemical techniques may be very useful for the detection of antigens of the dengue virus in infected tissues. The results obtained with the use of 2 IHC methods: one direct and another indirect amplified with a biotin universal antibody and a streptavidin peroxidase system (LSAB) (the peroxidase enzyme is used in both) are shown in this paper. These methods were applied on paraffin-embedded tissues from mice that were inoculated with the dengue virus intracerebrally. Our objective is to have a technique at the laboratory allowing to identify these antigens in the tissues in order to apply them on the diagnosis and on the studies connected with the obtention of immunogens.