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1.
J Vet Diagn Invest ; 33(5): 966-968, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34078202

RESUMO

We describe the molecular analysis of a wild-type field strain of bovine viral diarrhea virus (BVDV) identified in a mummified fetus from a small Brazilian dairy cattle herd. Nucleic acids extracted from samples of the lung, liver, heart, spleen, and kidney were tested by PCR assays for bovine alphaherpesvirus 1, Neospora caninum, Leptospira spp., Histophilus somni, and Brucella abortus, a nested PCR assay for Mycoplasma bovigenitalium and Ureaplasma diversum, and a RT-PCR assay for BVDV. Amplicons were only obtained in the RT-PCR assay for the partial amplification of the BVDV 5'UTR (288 bp) in kidney and spleen samples and the Npro (438 bp) gene in the kidney sample. Nucleotide sequencing of the amplified products and phylogenetic analyses based on the 2 BVDV genomic regions enabled the BVDV strain to be classified as subgenotype 1a.


Assuntos
Doença das Mucosas por Vírus da Diarreia Viral Bovina , Doenças dos Bovinos , Vírus da Diarreia Viral Bovina Tipo 1 , Vírus da Diarreia Viral Bovina , Animais , Bovinos , Diarreia/veterinária , Vírus da Diarreia Viral Bovina Tipo 1/genética , Vírus da Diarreia Viral Bovina/genética , Feto , Filogenia , Ureaplasma
2.
Braz J Microbiol ; 52(2): 961-965, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33483894

RESUMO

This study is aimed at detecting Feline paramyxovirus (FPaV) and Feline morbillivirus (FeMV) in 35 urine samples from domestic cats, collected in 2019, with or without clinical signs of uropathies using a reverse transcription-polymerase chain reaction (RT-PCR) followed by semi-nested polymerase chain reaction (SN-PCR) assays to amplify a partial paramyxovirus L gene. Eight (22.9%) out of the 35 urine samples were positive for paramyxoviruses. Sequencing and phylogenetic analyses revealed that three samples were positive for FPaV, four samples were positive for FeMV, and it was not possible to determine which virus was present in one RT-SN-PCR positive urine sample. FPaV strains showed 100% nucleotide (nt) identity with each other and 97% nt identity with a Japanese 163 FPaV strain. The FeMV strains showed 85.9% nt identity with each other; three strains were similar to previously described Brazilian FeMV strains, and one strain clustered in a different branch of the phylogenetic tree together with the first described Chinese FeMV strain. This study provides the first description of FPaV strains in cats from Brazil and provides new information about the molecular characteristics of FPaV and FeMV strains circulating in domestic cats in Brazil.


Assuntos
Doenças do Gato/virologia , Infecções por Paramyxoviridae/veterinária , Paramyxoviridae/genética , Animais , Animais Domésticos , Brasil/epidemiologia , Doenças do Gato/epidemiologia , Doenças do Gato/urina , Gatos , Morbillivirus/classificação , Morbillivirus/genética , Morbillivirus/isolamento & purificação , Paramyxoviridae/classificação , Paramyxoviridae/isolamento & purificação , Infecções por Paramyxoviridae/epidemiologia , Infecções por Paramyxoviridae/urina , Infecções por Paramyxoviridae/virologia , Filogenia , RNA Viral/genética , Análise de Sequência de DNA , Proteínas Virais/genética
3.
Comp Immunol Microbiol Infect Dis ; 71: 101494, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32434101

RESUMO

The etiological agents involved in a bovine respiratory disease (BRD) outbreak were investigated in a dairy heifer calf rearing unit from southern Brazil. A battery of PCR assays was performed to detect the most common viruses and bacteria associated with BRD, such as bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine alphaherpesvirus 1 (BoHV-1), bovine coronavirus (BCoV), bovine parainfluenza virus 3 (BPIV-3), Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis. Bronchoalveolar lavage fluid (BALF) samples were taken from 21 heifer calves (symptomatic n = 15; asymptomatic n = 6) that, during the occurrence of the BDR outbreak, were aged between 6 and 90 days. At least one microorganism was detected in 85.7 % (18/21) of the BALF samples. Mixed infections were more frequent (72.2 %) than single infections (27.7 %). The interactions between viruses and bacteria were the most common in coinfections (55.5 %). The frequencies of BRD agents were 38.1 % for BRSV, 28.6 % for BVDV, 33.3 % for BCoV, 42.85 % for P. multocida, 33.3 % for M. bovis, and 19 % for H. somni. BoHV-1, BPIV-3, and M. haemolytica were not identified in any of the 21 BALF samples. Considering that BALF and not nasal swabs were analyzed, these results demonstrate the etiological multiplicity that may be involved in BRD outbreaks in dairy calves.


Assuntos
Líquido da Lavagem Broncoalveolar/microbiologia , Doenças dos Bovinos/microbiologia , Surtos de Doenças/veterinária , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia , Animais , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/etiologia , Coronavirus Bovino/genética , Coronavirus Bovino/isolamento & purificação , Indústria de Laticínios , Vírus da Diarreia Viral Bovina/genética , Vírus da Diarreia Viral Bovina/isolamento & purificação , Técnicas de Diagnóstico Molecular/veterinária , Mycoplasma bovis/genética , Mycoplasma bovis/isolamento & purificação , Pasteurella multocida/genética , Pasteurella multocida/isolamento & purificação , Pasteurellaceae/genética , Pasteurellaceae/isolamento & purificação , Reação em Cadeia da Polimerase , Vírus Sincicial Respiratório Bovino/genética , Vírus Sincicial Respiratório Bovino/isolamento & purificação , Infecções Respiratórias/etiologia , Infecções Respiratórias/veterinária
4.
Arch Virol ; 165(8): 1855-1861, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32472289

RESUMO

In this report we describe the genotype constellation of a bovine rotavirus A (RVA) strain with an uncommon G8P[11] genotype combination. The RVA/Cow-wt/BRA/Y136/2017/G8P[11] strain was classified as G8-P[11]-I2-R5-C2-M2-A3-N2-T9-E2-H3. Phylogenetic analysis based on the VP7 gene showed that the Y136 strain and a human G8P[1] strain comprise a putative new (VII) lineage for the G8 genotype. In addition, two other genotypes, R5 (VP1) and T9 (NSP3), were identified in the constellation of Y136 that are rarely found in RVA strains of bovine origin. The immunological pressure caused by regular vaccination of cows might be responsible for the selection of heterologous RVA strains.


Assuntos
Infecções por Rotavirus/veterinária , Infecções por Rotavirus/virologia , Rotavirus/genética , Animais , Bovinos , Fezes/virologia , Gastroenterite/veterinária , Gastroenterite/virologia , Genoma Viral/genética , Genótipo , Humanos , Filogenia , Proteínas Virais/genética
5.
Ecohealth ; 16(2): 248-259, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31124021

RESUMO

Fibropapillomatosis is a panzootic and chronic disease among Chelonia mydas-usually associated with anthropogenic impacts. This study contributes towards understanding fibropapillomatosis implications for C. mydas populations as a reflector of environmental quality, via prevalence and histological, molecular and blood analyses at a World Heritage site in southern Brazil. Sixty-three juvenile C. mydas (31.3-54.5 cm curved carapace length-CCL) were sampled during two years. Eighteen specimens (~ 29%) had tumours (which were biopsied), while 45 had none. Degenerative changes in the epidermis and Chelonid alphaherpesvirus 5 DNA detection with three variants support a herpesvirus infection. Phylogenetic analysis indicated that variants A and B were similar to a herpesvirus lineage from the Atlantic group, but variant C was similar to a herpesvirus from the eastern Pacific lineage and represents the first published case for marine turtles off Brazil. Significantly lower levels of seven blood parameters, but greater numbers of eosinophils, were observed in tumour-afflicted animals. These observations were attributed to metabolism efficiencies and/or differences in diet associated with temporal-recruitment bias and disease development, and greater non-specific immune stimulation. While most animals had adequate body condition independent of disease, longer-term studies are required to elucidate any protracted population effects.


Assuntos
Alphaherpesvirinae/genética , DNA Viral/genética , Infecções por Herpesviridae/veterinária , Papiloma/veterinária , Neoplasias Cutâneas/veterinária , Tartarugas/virologia , Animais , Brasil , Infecções por Herpesviridae/virologia , Papiloma/virologia , Filogenia , Neoplasias Cutâneas/virologia
6.
Vet Microbiol ; 230: 260-264, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30827398

RESUMO

Worldwide, neonatal diarrhea is one of the most important health issues affecting dairy calves, and rotavirus A (RVA) is one of its primary causes. Among the measures to mitigate the risk of diarrhea outbreaks, cow vaccination stands out as one of the most important. However, the immune pressure resulting from routine vaccination may be able to select specific G and P genotypes in RVA field strains. This study aimed to determine the frequency and intensity of neonatal diarrhea and the incidence of RVA and attempted to monitor the G and P genotypes present in the RVA strains circulating in a high milk yield cattle herd vaccinated with RVA G6P[5] strain. Fecal samples (n = 1220) from 122 Holstein heifer calves between 0-30 days old that were born from RVA-vaccinated cows were collected at 10 different time points, regardless of the presence or absence of diarrhea. The presence of RVA in fecal samples was determined by the polyacrylamide gel electrophoresis (PAGE) technique and confirmed by reverse transcription polymerase chain reaction (RT-PCR). G and P amplicons from 10 RVA-positive fecal samples from calves of different ages and collections were subjected to nucleotide sequencing. The proportion of the calves and fecal samples that were positive for RVA were 62.3% (76/122) and 8.1% (99/1220), respectively. Using sequence analysis, all 10 RVA field strains presented genotype G10P[11]. The protection of G6P[5] vaccination is clear, as this genotype was not detected in this study, and it is known that vaccination against RVA reduces the incidence of diarrhea independent of genotype involved. This result demonstrates the importance of epidemiological monitoring of RVA genotypes circulating in vaccinated dairy cattle herds to the early detection of new potential pathogenic RVA strains.


Assuntos
Doenças dos Bovinos/virologia , Bovinos/virologia , Infecções por Rotavirus/veterinária , Vacinas contra Rotavirus/administração & dosagem , Rotavirus/genética , Animais , Animais Recém-Nascidos , Doenças dos Bovinos/epidemiologia , Indústria de Laticínios , Diarreia/virologia , Monitoramento Epidemiológico/veterinária , Fezes/virologia , Genótipo , Estudos Longitudinais , Leite , Filogenia , Rotavirus/patogenicidade , Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/prevenção & controle , Análise de Sequência de DNA , Vacinação/veterinária
7.
Trop Anim Health Prod ; 51(4): 887-892, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30535644

RESUMO

Neonatal diarrhea is the main cause of morbidity and mortality in calves up to 30 days old, and rotavirus A (RVA) is the main viral etiology. RVA vaccines are one of the main tools for diarrhea control in neonates. The aim of this cross-sectional study was to monitor by RT-PCR the G and P genotypes of RVA strains identified in dairy cattle herds regularly vaccinated with the RVA UK strain (G6P[5]). Of the 14 randomly selected herds, two were excluded because no calf was diagnosed with diarrhea on the day of fecal collection. Another six herds were also excluded from the study because all 20 diarrheic fecal samples evaluated were RT-PCR-negative. In the remaining six herds, 17 (25.4%) of the 67 diarrheic samples were RVA-positive. One G and P amplicon from each herd were selected for nucleotide sequencing. In the phylogenetic analysis, five RVA strains presented the G6P[11] genotype, and one presented the G10P[11] genotype. The G6 genotype present in all RVA field strains clustered into a distinct phylogenetic arrangement (lineage III) of the UK vaccine strain (lineage IV), characterizing the emergence of a phylogenetically distant G6 strain. In addition, we observed the emergence of strains with G10 and P[11] genotypes characterizing failure in heterologous immune protection. These results show the epidemiological importance of constant monitoring of RVA strains in vaccinated cattle herds and the low frequencies of diarrhea and diagnosis of RVA suggest that a regular vaccination program reduces the frequency and severity of RVA diarrhea in suckling calves.


Assuntos
Doenças dos Bovinos/virologia , Diarreia/veterinária , Infecções por Rotavirus/veterinária , Vacinas contra Rotavirus , Rotavirus/genética , Animais , Animais Recém-Nascidos , Sequência de Bases , Bovinos , Doenças dos Bovinos/prevenção & controle , Estudos Transversais , Diarreia/prevenção & controle , Diarreia/virologia , Fezes/virologia , Genótipo , Filogenia , Infecções por Rotavirus/prevenção & controle , Infecções por Rotavirus/virologia
8.
Pesqui. vet. bras ; 38(8): 1681-1684, Aug. 2018. tab
Artigo em Inglês | VETINDEX | ID: vti-22319

RESUMO

The occurrence of antibodies against canine distemper virus (CDV), parvovirus and Ehrlichia spp. in wild captive carnivores was evaluated in a zoological park in midwestern Brazil. Serum samples were collected between 2007 and 2014 from 45 carnivores. Antibodies were evaluated by virus neutralization assay for CDV, hemagglutination inhibition test for parvovirus, indirect immunofluorescent and Enzyme-linked immunosorbent assay for Ehrlichia spp. Antibodies against CDV and parvovirus were detected in 75% of Canidae and Felidae. Procyonidae were negative for CDV, although one Mustelidae was positive. TwoCanidae presented antibodies reactive to E. canis antigens. The high antibodies rates to CDV and parvovirus suggest the contact with both pathogens, however since no clinical history of disease are registered in the Zoo-UFMT, we can presume that carnivores have responded satisfactorily against the antigens. The low serological rates observed against Ehrlichia spp. may be resulted to the low occurrence of ticks among carnivores.(AU)


A ocorrência de anticorpos contra o vírus da cinomose canina (CDV), parvovírus e Ehrlichia spp. em carnívoros selvagens em cativeiro foi avaliada em um parque zoológico do centro oeste do Brasil. As amostras de soro foram coletadas entre 2007 e 2014 de 45 carnívoros. Os anticorpos foram avaliados por ensaio de neutralização de vírus para CDV, teste de inibição de hemaglutinação para parvovírus, imunofluorescência indireta e ensaio imunoenzimático ligado à enzima para Ehrlichia spp. Anticorpos contra CDV e parvovírus foram detectados em 75% de canídeos e felídeos. Procionídeos foram negativos para CDV, embora um mustelídeo fora positivo. Dois canídeos apresentaram anticorpos reativos aos antígenos de E. canis. As altas taxas de anticorpos para CDV e parvovírus sugerem o contato com ambos os patógenos, entretanto desde que nenhuma história clínica de doença está registrada no Zoo-UFMT, podemos presumir que os carnívoros têm respondido satisfatoriamente contra os antígenos. As baixas taxas serológicas observadas contra Ehrlichia spp. pode ser resultado da baixa ocorrência de carrapatos entre os carnívoros.(AU)


Assuntos
Animais , Carnívoros/imunologia , Parvovirus/patogenicidade , Cinomose/imunologia , Ehrlichia/patogenicidade
9.
Pesqui. vet. bras ; Pesqui. vet. bras;38(8): 1681-1684, Aug. 2018. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-976467

RESUMO

The occurrence of antibodies against canine distemper virus (CDV), parvovirus and Ehrlichia spp. in wild captive carnivores was evaluated in a zoological park in midwestern Brazil. Serum samples were collected between 2007 and 2014 from 45 carnivores. Antibodies were evaluated by virus neutralization assay for CDV, hemagglutination inhibition test for parvovirus, indirect immunofluorescent and Enzyme-linked immunosorbent assay for Ehrlichia spp. Antibodies against CDV and parvovirus were detected in 75% of Canidae and Felidae. Procyonidae were negative for CDV, although one Mustelidae was positive. TwoCanidae presented antibodies reactive to E. canis antigens. The high antibodies rates to CDV and parvovirus suggest the contact with both pathogens, however since no clinical history of disease are registered in the Zoo-UFMT, we can presume that carnivores have responded satisfactorily against the antigens. The low serological rates observed against Ehrlichia spp. may be resulted to the low occurrence of ticks among carnivores.(AU)


A ocorrência de anticorpos contra o vírus da cinomose canina (CDV), parvovírus e Ehrlichia spp. em carnívoros selvagens em cativeiro foi avaliada em um parque zoológico do centro oeste do Brasil. As amostras de soro foram coletadas entre 2007 e 2014 de 45 carnívoros. Os anticorpos foram avaliados por ensaio de neutralização de vírus para CDV, teste de inibição de hemaglutinação para parvovírus, imunofluorescência indireta e ensaio imunoenzimático ligado à enzima para Ehrlichia spp. Anticorpos contra CDV e parvovírus foram detectados em 75% de canídeos e felídeos. Procionídeos foram negativos para CDV, embora um mustelídeo fora positivo. Dois canídeos apresentaram anticorpos reativos aos antígenos de E. canis. As altas taxas de anticorpos para CDV e parvovírus sugerem o contato com ambos os patógenos, entretanto desde que nenhuma história clínica de doença está registrada no Zoo-UFMT, podemos presumir que os carnívoros têm respondido satisfatoriamente contra os antígenos. As baixas taxas serológicas observadas contra Ehrlichia spp. pode ser resultado da baixa ocorrência de carrapatos entre os carnívoros.(AU)


Assuntos
Animais , Carnívoros/imunologia , Parvovirus/patogenicidade , Cinomose/imunologia , Ehrlichia/patogenicidade
10.
Vet Res Commun ; 41(4): 317-321, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28836073

RESUMO

Seneca Valley virus (SVV) is the etiological agent of vesicular disease in pigs, clinically indistinguishable of classical viral vesicular infections, including foot-and-mouth disease. The first outbreaks of SVV infection in Brazil were reported in 2014. However, it was not known whether the virus was circulating in Brazilian pig herds before this year. This study is a retrospective serological investigation of porcine health status to SVV in Brazil. Serum samples (n = 594) were grouped in before (2007-2013, n = 347) and after (2014-2016, n = 247) SVV outbreaks in Brazil. Twenty-three pig herds were analyzed, of which 19 and 4 were sampled before and after the beginning of SVV outbreaks, respectively. Two herds sampled after 2014 presented animals with SVV-associated clinical manifestations, while the other two housed asymptomatic pigs. Anti-SVV antibodies were evaluated by virus neutralization test. The results demonstrated that pig herds of different Brazilian geographical regions and distinct pig categories were negative to anti-SVV antibodies in sera obtained before 2014. Antibodies to SVV were detected only in serum samples obtained after 2014, particularly in herds with the presence of pigs with SVV-clinical signs. These results present robust serological evidence that the SVV was not present in the major Brazilian pig producing regions prior to 2014.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Picornaviridae/veterinária , Doenças dos Suínos/epidemiologia , Animais , Brasil/epidemiologia , Testes de Neutralização , Picornaviridae/genética , Picornaviridae/imunologia , Infecções por Picornaviridae/epidemiologia , Estudos Retrospectivos , Estudos Soroepidemiológicos , Suínos , Doenças dos Suínos/virologia
11.
Trop Anim Health Prod ; 46(1): 19-25, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23832703

RESUMO

Reports of bovine listeriosis in Brazil are uncommon, being restricted to citations within retrospective studies, resulting in scarce documented information of this important disease of cattle. This manuscript describes the molecular findings associated with spontaneous encephalitic listeriosis in two steers from distinct herds within the state of Paraná, southern Brazil. Both animals demonstrated altered consciousness suggestive of brain stem dysfunctions and died a few days after the initial onset of disease. Polymerase chain reaction (PCR) assays were designed to target specific genes of infectious neurological agents of cattle. These included bovine herpesvirus 1 and 5 (BoHV-1 and BoHV-5), ovine herpesvirus 2 (OvHV-2), Listeria monocytogenes, and Histophilus somni. Rabies virus was discarded in evaluations done at the official state diagnostic laboratory. Gross alterations were insignificant; histopathology demonstrated rhombencephalitis associated with macrophage-predominant, multifocal to coalescing microabscesses and extensive perivascular cuffings in both steers. The L. monocytogenes PCR assay amplified the 172-bp amplicon of the listeriolysin gene from the brain stem of both animals and from the telencephalon, thalamus, and cerebellum of one of them. Phylogenetic analyses demonstrated that the strains derived from this study clustered with known strains of L. monocytogenes lineage I. The BoHV-1 and BoHV-5, OvHV-2, and H. somni PCR assays were negative. These results confirm the participation of L. monocytogenes lineage I in the etiopathogenesis of the neurological disease herein described and represent the first complete description of encephalitic listeriosis in cattle from Brazil.


Assuntos
Doenças dos Bovinos/microbiologia , Encefalite/veterinária , Listeria monocytogenes/genética , Listeriose/veterinária , Animais , Tronco Encefálico/microbiologia , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Encefalite/epidemiologia , Encefalite/microbiologia , Feminino , Listeria monocytogenes/classificação , Listeria monocytogenes/isolamento & purificação , Listeriose/epidemiologia , Listeriose/microbiologia , Masculino , Filogenia , Reação em Cadeia da Polimerase/veterinária
12.
Braz. J. Microbiol. ; 44(3): 889-896, July-Sept. 2013.
Artigo em Inglês | VETINDEX | ID: vti-304345

RESUMO

Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions, the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions, significant histopathological alterations were restricted to the brainstem (medulla oblongata, rhombencephalitis) and were characterized as meningoencephalitis that consisted of extensive mononuclear perivascular cuffings, neutrophilic and macrophagic microabscesses, and neuroparenchymal necrosis. PCR assay and direct sequencing, using genomic bacterial DNA derived from the brainstem of both animals, amplified the desired 174 base pairs length amplicon of the listeriolysin O gene of L. monocytogenes. Phylogenetic analyses demonstrated that the strains associated with rhombencephalitis during this study clustered with known strains of L. monocytogenes lineage I from diverse geographical locations and from cattle of the state of Paraná with encephalitic listeriosis. Consequently, these strains should be classified as L. monocytogenes lineage I. These results confirm the active participation of lineage I strains of L. monocytogenes in the etiopathogenesis of the brainstem dysfunctions observed during this study, probably represent the first characterization of small ruminant listeriosis by molecular techniques in Latin America, and suggest that ruminants within the state of Paraná were infected by the strains of the same lineage of L. monocytogenes.(AU)


Assuntos
Listeriose , Encefalite , Ruminantes , Coloração e Rotulagem , Filogenia
13.
Braz. j. microbiol ; Braz. j. microbiol;44(3): 889-896, July-Sept. 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-699822

RESUMO

Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions; the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions; significant histopathological alterations were restricted to the brainstem (medulla oblongata; rhombencephalitis) and were characterized as meningoencephalitis that consisted of extensive mononuclear perivascular cuffings, neutrophilic and macrophagic microabscesses, and neuroparenchymal necrosis. PCR assay and direct sequencing, using genomic bacterial DNA derived from the brainstem of both animals, amplified the desired 174 base pairs length amplicon of the listeriolysin O gene of L. monocytogenes. Phylogenetic analyses demonstrated that the strains associated with rhombencephalitis during this study clustered with known strains of L. monocytogenes lineage I from diverse geographical locations and from cattle of the state of Paraná with encephalitic listeriosis. Consequently, these strains should be classified as L. monocytogenes lineage I. These results confirm the active participation of lineage I strains of L. monocytogenes in the etiopathogenesis of the brainstem dysfunctions observed during this study, probably represent the first characterization of small ruminant listeriosis by molecular techniques in Latin America, and suggest that ruminants within the state of Paraná were infected by the strains of the same lineage of L. monocytogenes.


Assuntos
Animais , Feminino , Masculino , Toxinas Bacterianas/genética , Doenças das Cabras/patologia , Proteínas de Choque Térmico/genética , Proteínas Hemolisinas/genética , Listeriose/veterinária , Meningoencefalite/veterinária , Doenças dos Ovinos/patologia , Brasil , Tronco Encefálico/patologia , Análise por Conglomerados , Genótipo , Cabras , Doenças das Cabras/microbiologia , Histocitoquímica , Listeria monocytogenes/genética , Listeriose/microbiologia , Listeriose/patologia , Meningoencefalite/microbiologia , Meningoencefalite/patologia , Filogenia , Reação em Cadeia da Polimerase , Homologia de Sequência , Ovinos , Doenças dos Ovinos/microbiologia
14.
Braz J Microbiol ; 44(3): 889-96, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24516457

RESUMO

Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions; the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions; significant histopathological alterations were restricted to the brainstem (medulla oblongata; rhombencephalitis) and were characterized as meningoencephalitis that consisted of extensive mononuclear perivascular cuffings, neutrophilic and macrophagic microabscesses, and neuroparenchymal necrosis. PCR assay and direct sequencing, using genomic bacterial DNA derived from the brainstem of both animals, amplified the desired 174 base pairs length amplicon of the listeriolysin O gene of L. monocytogenes. Phylogenetic analyses demonstrated that the strains associated with rhombencephalitis during this study clustered with known strains of L. monocytogenes lineage I from diverse geographical locations and from cattle of the state of Paraná with encephalitic listeriosis. Consequently, these strains should be classified as L. monocytogenes lineage I. These results confirm the active participation of lineage I strains of L. monocytogenes in the etiopathogenesis of the brainstem dysfunctions observed during this study, probably represent the first characterization of small ruminant listeriosis by molecular techniques in Latin America, and suggest that ruminants within the state of Paraná were infected by the strains of the same lineage of L. monocytogenes.


Assuntos
Toxinas Bacterianas/genética , Doenças das Cabras/patologia , Proteínas de Choque Térmico/genética , Proteínas Hemolisinas/genética , Listeriose/veterinária , Meningoencefalite/veterinária , Doenças dos Ovinos/patologia , Animais , Tronco Encefálico/patologia , Brasil , Análise por Conglomerados , Feminino , Genótipo , Doenças das Cabras/microbiologia , Cabras , Histocitoquímica , Listeria monocytogenes/genética , Listeriose/microbiologia , Listeriose/patologia , Masculino , Meningoencefalite/microbiologia , Meningoencefalite/patologia , Filogenia , Reação em Cadeia da Polimerase , Homologia de Sequência , Ovinos , Doenças dos Ovinos/microbiologia
15.
Braz. j. microbiol ; Braz. j. microbiol;40(1): 199-204, Jan.-Mar. 2009. tab
Artigo em Inglês | LILACS | ID: lil-513142

RESUMO

Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genesdetected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) andSTx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form werefound and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.


A identificação de amostras de Escherichia coli responsáveis por diarréia pós-desmame em suínos requerconhecimento dos patotipos prevalentes dentro de uma dada região. Cem amostras de Escherichia coli isoladas de leitões com diarréia no Estado do Paraná, Brasil, foram testadas para apresença dos genes que codificam antígenos fimbriais F4, F5, F6, F18, F41 e para a produção de enterotoxinas (STa, STb, LT and STx2e), através de sondas e da técnica da PCR (polymerasechain reaction). Os resultados mostraram que 60% dos isolados foram positivos para um ou mais antígenos fimbriais e 92% foram positivos para pelo menos um dos fatores de virulência examinados. Os genes de virulência detectados foram F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa(40%), STb (47%) e STx2e (3%). Vinte e quatro padrões de virulência, de acordo com as diferentes combinações dos genes de virulência, foram encontrados e o mais prevalente foi F4,F18, F41, STa, STb e LT. A maioria das amostras que carreiam genes para adesinas também transportam genes para produção de toxinas.


Assuntos
Animais , Diarreia , Infecções por Escherichia coli , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Frequência do Gene , Técnicas In Vitro , Reação em Cadeia da Polimerase , Suínos , Métodos , Técnicas e Procedimentos Diagnósticos , Virulência
16.
Braz J Microbiol ; 40(1): 199-204, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24031344

RESUMO

Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form were found and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.

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