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DNA Repair (Amst) ; 59: 20-26, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28938097

RESUMO

imuABC (imuAB dnaE2) genes are responsible for SOS-mutagenesis in Caulobacter crescentus and other bacterial species devoid of umuDC. In this work, we have constructed operator-constitutive mutants of the imuABC operon. We used this genetic tool to investigate the effect of SOS-induced levels of these genes upon both spontaneous and damage-induced mutagenesis. We showed that constitutive expression of imuABC does not increase spontaneous or damage-induced mutagenesis, nor increases cellular resistance to DNA-damaging agents. Nevertheless, the presence of the operator-constitutive mutation rescues mutagenesis in a recA background, indicating that imuABC are the only genes required at SOS-induced levels for translesion synthesis (TLS) in C. crescentus. Furthermore, these data also show that TLS mediated by ImuABC does not require RecA, unlike umuDC-dependent mutagenesis in E. coli.


Assuntos
Caulobacter crescentus/metabolismo , Dano ao DNA , Replicação do DNA , RNA Polimerases Dirigidas por DNA/metabolismo , Mutagênese , Resposta SOS em Genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Caulobacter crescentus/genética , DNA/metabolismo , RNA Polimerases Dirigidas por DNA/genética
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