RESUMO
Cryopreservation of human ovarian tissue represents a key procedure for fertility preservation. The two most widely used cryopreservation methods for human ovarian cortex samples are slow freezing\thawing (SF\T) and vitrification\warming (V\W). The aim of the present study was to analyze the effects of SF\T and V\W using a metal chamber, on specific follicle and oocyte structures and on the stromal organization post-cryopreservation. We did histology analysis of SF\T and V\W ovarian fragments from nine healthy subjects. Overall results showed that cryopreserved tissues presented significant rates of damage in primordial and primary follicles. Altered nuclear structure of primordial follicles and cell detachment from primordial and primary follicles were the main injuries observed after V/W and SF/T. The stromal components were similarly well preserved after cryopreservation. We conclude that both cryopreservation methods may be used for fertility preservation purposes with similar outcomes in terms of follicular and stromal integrity. Detachment of follicle cells from basal membrane represents an important cryoinjury that deserves further investigation.
Assuntos
Preservação da Fertilidade , Vitrificação , Criopreservação/métodos , Feminino , Preservação da Fertilidade/métodos , Congelamento , Humanos , Folículo OvarianoRESUMO
Cryopreservation of ovarian tissue is known to affect follicular survival. Several variables may be responsible for this. Little attention has focused on the effect of the size of the fragment to be cryopreserved. This study was conducted to assess the effect of the size of the tissue on follicular histology after freezing with 1,2-propanediol. Histological evaluations were performed of control and cryopreserved tissue. Fragments were cut 10 x 3 x 2 mm(3) (2 mm group) or 10 x 3 x 4 mm(3) (4 mm group). Percentages of normal follicles in control fragments cut into 2 and 4 mm slices were 56% and 34%, respectively. The relative risks to obtain normal follicles in the 2 mm and the 4 mm fragments after cryopreservation were 0.63 and 0.47, respectively. Freezing reduced follicle survival to a significantly greater extent in the larger tissue fragments. There is an increased risk of damage to primary and primordial follicles, when the tissue slices are cut with all dimensions larger than 2 mm.
Assuntos
Bovinos , Criopreservação/veterinária , Ovário/fisiologia , Animais , Criopreservação/métodos , Feminino , Folículo Ovariano/anatomia & histologia , Folículo Ovariano/fisiologia , Ovário/anatomia & histologia , Sobrevivência de Tecidos/fisiologia , Coleta de Tecidos e Órgãos/métodos , Coleta de Tecidos e Órgãos/veterináriaRESUMO
A transvaginal Doppler sonographic examination of uterine artery blood flow was performed. Resistance and pulsatility indices were measured in 101 women, 74 of whom were using an intrauterine contraceptive device, and 27 controls, who were not using any contraceptive method. The intrauterine contraceptive device users were divided into three groups: those with normal bleeding (n = 34); those with abnormal uterine bleeding without medication (n = 16); and those with abnormal bleeding corrected with use of prostaglandin inhibitors (n = 24). The resistance and pulsatility values were significantly lower in the group of women using intrauterine contraceptive devices who had abnormal bleeding than in all other groups (P<0.001). All other comparisons were nonsignificant. A pulsatility index of less than 2 may be associated with a higher risk for development of intrauterine contraceptive device-induced bleeding.
Assuntos
Dispositivos Intrauterinos , Fluxo Pulsátil/fisiologia , Ultrassonografia Doppler em Cores , Útero/irrigação sanguínea , Resistência Vascular/fisiologia , Adulto , Artérias/diagnóstico por imagem , Diagnóstico Diferencial , Feminino , Humanos , Pessoa de Meia-Idade , Valores de Referência , Fatores de Risco , Hemorragia Uterina/diagnóstico por imagem , Hemorragia Uterina/etiologiaRESUMO
Resultado de 129 punçöes da vilosidade coriônica para diagnóstico pré-natal no primeiro trimestre de gestaçäo. Os métodos de rotina par preparaçäo direta, preparaçäo de 24 horas e cultura de longa duraçäo, bem como as indicaçöes säo descritos detalhadamente. Tendo em vista a precocidade desse exame e alto sucesso de técnica (97,4%), pode-se concluir pela sua aceitabilidade como método complementar de diagnóstico genético pré-natal