RESUMO
Data emerged from the last 20 years of basic research on tumor antigens positioned the type I MAGE (Melanoma Antigen GEnes - I or MAGE-I) family as cancer driver factors. MAGE-I gene expression is mainly restricted to normal reproductive tissues. However, abnormal re-expression in cancer unbalances the cell status towards enhanced oncogenic activity or reduced tumor suppression. Anomalous MAGE-I gene re-expression in cancer is attributed to altered epigenetic-mediated chromatin silencing. Still, emerging data indicate that MAGE-I can be regulated at protein level. Results from different laboratories suggest that after its anomalous re-expression, specific MAGE-I proteins can be regulated by well-known signaling pathways or key cellular processes that finally potentiate the cancer cell phenotype. Thus, MAGE-I proteins both regulate and are regulated by cancer-related pathways. Here, we present an updated review highlighting the recent findings on the regulation of MAGE-I by oncogenic pathways and the potential consequences in the tumor cell behavior.
Assuntos
Melanoma , Proteínas de Neoplasias , Humanos , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Antígenos de Neoplasias/genéticaRESUMO
OBJETIVO: El objetivo del estudio fue describir las dinámicas comunitarias y gubernamentales en torno a la malaria, su control y prevención en el Municipio de San José del Guaviare, en el departamento de Guaviare (Colombia). MÉTODOS: Se realizó un estudio de métodos mixtos utilizando una encuesta que constaba de 45 preguntas sobre conocimientos, actitudes y prácticas y entrevistas semiestructuradas con personal médico y trabajadores del gobierno. RESULTADOS: Aceptaron realizar la encuesta 103 personas, el 72% fueron mujeres, edad promedio de 40,8 años, tiene acueducto el 60% y alcantarillado 53,4%. El 59% reportó haber tenido malaria, 76% reconoció un mosquito como el vector de la enfermedad, 6% reconoce al Anopheles como el vector. El 16,5% de los encuestados asegura que la malaria no es un problema de salud para ellos y sus familias. El uso de toldillos (74%) es considerado una eficaz protección. CONCLUSIONES: Se evidencia un buen conocimiento de la malaria después de los acuerdos de paz, sin embargo, los determinantes relacionados con suministro de agua, alcantarillado, difícil atención en zona rural y las escasas acciones interdisciplinarias son posibles elementos de riesgo para esta población.
The objective of the study was to describe the community and government dynamics around malaria, especially its control and prevention, in the Municipality of San José del Guaviare, in the sector of Guaviare (Colombia). METHODS: We conducted a mixed methods study using a survey consisting of 45 questions on knowledge, attitudes, and practices, and semi-structured interviews with medical personnel and government workers. RESULTS: 103 people responded to the survey. 72% were women, and the average age was 40.8 years. 60% had an aqueduct and 53.4% had sewers. 59% reported having had malaria, 76% recognized a mosquito as the vector of the disease, and 6% recognized Anopheles as the vector. 16.5% of those surveyed said that malaria was not a health problem for them and their families. 74% considered the use of mosquito nets an effective protection. CONCLUSIONS: While there is a good knowledge of malaria, difficulties related to water supply, sewage, care in rural areas and lack of interdisciplinary solutions pose possible risk factors for this population.
Assuntos
Humanos , Masculino , Feminino , Adulto , Conhecimento , Malária/prevenção & controle , Colômbia/epidemiologiaRESUMO
Introduction: In Colombia, malaria affects people in vulnerable conditions. In urban areas, disease control measures have decreased the incidence of malaria in the past 5 years. However, there is some government abandonment in areas with armed conflict. Objective: To determine the knowledges, attitudes and practice regarding malaria in Piñalito, a town located in the municipality of Vista Hermosa in the Meta department (Colombia), in the period 2016-2017. Methods: We conducted a mixed-methods study using a survey consisting of 45 questions on knowledge, attitudes, and practices and semistructured interviews with medical personnel and government workers. Results: Of the respondents, 89.13% said that malaria is a health problem, 63.04% said the disease is transmitted by a mosquito bite, main symptoms were reported to be high fever (78.26%) and headache (60.87%). A total of 73.91% of respondents affirmed using mosquito nets to avoid the disease. The community affirmed that they do not receive good medical attention (65.22%) and did not identify the active participation of the local health secretary to prevent malaria. Conclusions: The inhabitants of Piñalito demonstrated adequate knowledge about the disease and positive attitudes toward preventing malaria. However, they had negative perceptions about the actions of the secretary of health and the government for control and prevention of the disease.
Introducción: En Colombia, la malaria afecta a personas en condiciones de vulnerabilidad. En áreas urbanas, las medidas de control de enfermedades han disminuido la incidencia de malaria en los últimos 5 años. Sin embargo, existe cierto abandono del gobierno en áreas con conflicto armado. Objetivo: Determinar los conocimientos, actitudes y prácticas sobre la malaria en la población del municipio de Piñalito (Meta, Colombia), en el periodo 2016-2017. Métodos: Realizamos un estudio de métodos mixtos utilizando una encuesta de 45 preguntas sobre conocimientos, actitudes y prácticas de la población y entrevistas semiestructuradas con personal médico y trabajadores del gobierno. Resultados: De los encuestados, el 89,13 % dijo que la malaria es un problema de salud, el 63,04 % considera que la enfermedad se transmite por la picadura de un mosquito. Los síntomas principales fueron fiebre alta (78,26 %) y dolor de cabeza (60,87 %). El 73,91 % de los encuestados afirmó utilizar mosquiteros para evitar la enfermedad. La comunidad manifestó que no recibe buena atención médica (65,22 %) y no identificó la participación activa de la secretaría de salud local para prevenir la malaria. Conclusiones: Los habitantes de Piñalito demostraron un adecuado conocimiento sobre la enfermedad y actitudes positivas hacia la prevención de la malaria. Sin embargo, tenían percepciones negativas sobre las acciones de la secretaría de salud y del gobierno para el control y prevención de la enfermedad.
Assuntos
Humanos , Estudos Retrospectivos , Estudos LongitudinaisRESUMO
Introduction: Paraquat is an agrochemical that constitutes the first cause of death by poisoning in different countries. Objective: To relate sociodemographic and clinical characteristics with the final condition of patients intoxicated by paraquat in a hospital in southwestern Colombia. Materials and methods: This was an observational, descriptive, analytical, crosssectional, and retrospective study. We reviewed the medical records of patients diagnosed with paraquat poisoning at a level III hospital in Pasto, Colombia, from 2013 to 2018. We collected the data regarding their final condition (alive or deceased) and their sociodemographic, clinical, and paraclinical information. We established group comparisons and designed a binary logistic regression model. Results: We reviewed 299 records and, after the exclusion, we analyzed 160 cases. The characteristics related to the final condition of the patients were length of stay (OR = 0.124; 95% CI: 0.03-0.6; p = 0.009), heart rate (OR = 35.65; 95% CI: 1.44-884.78; p = 0.029), and initial creatinine (OR = 1.73; 95% CI: 1.23-2.44; p = 0.002). Conclusion: The proportion of case fatality was higher in patients with short hospital stay times and elevated heart rates and admission creatinine levels. This report may be useful as a rationale for the creation of a severity scale for the early detection of patients with adverse outcomes and their timely treatment.
Introducción. El herbicida paraquat constituye la primera causa de decesos por intoxicaciones en distintos países. Objetivo. Relacionar las características sociodemográficas y clínicas con la condición final de pacientes intoxicados por paraquat atendidos en un hospital del suroccidente de Colombia. Materiales y métodos. En este estudio observacional, descriptivo, analítico, transversal y retrospectivo, se revisaron las historias clínicas de pacientes atendidos por intoxicación con paraquat en una institución de tercer nivel de complejidad en Pasto (Colombia) entre el 2013 y el 2018. Se recolectó la información sobre la condición final (vivo o fallecido) de cada paciente, así como los datos sociodemográficos, clínicos y de los exámenes paraclínicos. Se establecieron comparaciones por grupos y se diseñó un modelo de regresión logística binaria. Resultados. Se filtró la información de 299 registros y se analizaron finalmente 160 casos. Las características relacionadas con la condición final de los pacientes fueron el tiempo de estancia (OR=0,124; IC95% 0,03-0,6; p=0,009), la frecuencia cardiaca (OR=35,65; IC95% 1,44-884,78; p=0,029) y la creatinina inicial (OR=1,73; IC95% 1,23-2,44; p=0,002). Conclusiones. La proporción de la letalidad fue mayor entre los pacientes con estancia hospitalaria corta, taquicardia y elevación de la concentración sérica de creatinina al ingreso. Este reporte puede ser útil como fundamento de una escala de gravedad para detectar a los pacientes con resultados adversos en la fase temprana para que puedan recibir una intervención oportuna.
Assuntos
Herbicidas , Paraquat , Agroquímicos , Colômbia/epidemiologia , Creatinina , Hospitais , Humanos , Estudos Observacionais como Assunto , Estudos RetrospectivosRESUMO
Introducción. El herbicida paraquat constituye la primera causa de decesos por intoxicaciones en distintos países. Objetivo. Relacionar las características sociodemográficas y clínicas con la condición final de pacientes intoxicados por paraquat atendidos en un hospital del suroccidente de Colombia. Materiales y métodos. En este estudio observacional, descriptivo, analítico, transversal y retrospectivo, se revisaron las historias clínicas de pacientes atendidos por intoxicación con paraquat en una institución de tercer nivel de complejidad en Pasto (Colombia) entre el 2013 y el 2018. Se recolectó la información sobre la condición final (vivo o fallecido) de cada paciente, así como los datos sociodemográficos, clínicos y de los exámenes paraclínicos. Se establecieron comparaciones por grupos y se diseñó un modelo de regresión logística binaria. Resultados. Se filtró la información de 299 registros y se analizaron finalmente 160 casos. Las características relacionadas con la condición final de los pacientes fueron el tiempo de estancia (OR=0,124; IC95% 0,03-0,6; p=0,009), la frecuencia cardiaca (OR=35,65; IC95% 1,44-884,78; p=0,029) y la creatinina inicial (OR=1,73; IC95% 1,23-2,44; p=0,002). Conclusiones. La proporción de la letalidad fue mayor entre los pacientes con estancia hospitalaria corta, taquicardia y elevación de la concentración sérica de creatinina al ingreso. Este reporte puede ser útil como fundamento de una escala de gravedad para detectar a los pacientes con resultados adversos en la fase temprana para que puedan recibir una intervención oportuna.
Introduction: Paraquat is an agrochemical that constitutes the first cause of death by poisoning in different countries. Objective: To relate sociodemographic and clinical characteristics with the final condition of patients intoxicated by paraquat in a hospital in southwestern Colombia. Materials and methods: This was an observational, descriptive, analytical, crosssectional, and retrospective study. We reviewed the medical records of patients diagnosed with paraquat poisoning at a level III hospital in Pasto, Colombia, from 2013 to 2018. We collected the data regarding their final condition (alive or deceased) and their sociodemographic, clinical, and paraclinical information. We established group comparisons and designed a binary logistic regression model. Results: We reviewed 299 records and, after the exclusion, we analyzed 160 cases. The characteristics related to the final condition of the patients were length of stay (OR = 0.124; 95% CI: 0.03-0.6; p = 0.009), heart rate (OR = 35.65; 95% CI: 1.44-884.78; p = 0.029), and initial creatinine (OR = 1.73; 95% CI: 1.23-2.44; p = 0.002). Conclusion: The proportion of case fatality was higher in patients with short hospital stay times and elevated heart rates and admission creatinine levels. This report may be useful as a rationale for the creation of a severity scale for the early detection of patients with adverse outcomes and their timely treatment.
Assuntos
Paraquat , Intoxicação , Praguicidas , Mortalidade , Creatinina , HerbicidasRESUMO
Low red-LED irradiances are an attractive alternative for enhancing microalgae photobioreactors treating digestate due to their potential contribution in decreasing area footprints with low energy consumptions. However, more information is required regarding the influence of digestate load on treatment performance and biomass valorisation when low-intensity red-LEDs are applied. Thus, this study assessed microalgae-based photobioreactors treating food waste digestate under different concentrations (5%, 25%, 50%, and 75%, v/v) at low red-LED irradiance (15 µmol·m-2·s-1). The removal efficiencies of soluble chemical oxygen demand (sCOD) at the end of the experiment ranged from 45% to 75% when treating influent loads between 5.3 and 79.1â g sCOD·m-3·d-1 (5% and 75%-digestate), respectively. Total ammonia nitrogen (TAN) was applied in loading rates between 3.2 and 48.5â g TAN·m-3·d-1 (5% and 75%, respectively) and removed with maximum efficiencies of 90%-100% in all trials. Nitrification-denitrification was proportionally more relevant when treating 5%-digestate, whereas volatilisation was the primary process in 25%, 50% and 75% concentrations. Microalgae presented adequate yields in all treatments, except in 75%-digestate, likely due to the blocking of light by the high solids concentrations. The assessment of the microalgae community and chlorophyll-a and carotenoids suggested that chlorophytes, mainly Dictyosphaerium pulchellum and Scenedesmus sp. grew autotrophically, whereas cyanobacteria Pseudanabaena sp. grew mixotrophically. Moreover, the sustainability of red LED lighting applications can be increased by anaerobic digestion or agricultural valorisation of the biomass, enabled by its high N and P contents. Low-intensity red-LEDs may have promissory applications in the treatment of high-strength wastewaters.
Assuntos
Microalgas , Eliminação de Resíduos , Biomassa , Alimentos , Microalgas/química , Nitrogênio , Fotobiorreatores , Águas Residuárias/químicaRESUMO
RESUMEN Introducción: Existen más de 390 millones de personas infectadas y 20 000 muertes anualmente a causa del dengue en el mundo. El chikungunya ocasionó una larga epidemia en las Américas con más de 2 millones y medio de casos hasta el 2017. Objetivo: Identificar las características sociodemográficas y clínicas de los casos de dengue y chikungunya informados para el periodo 2014-2017 en Nariño, Colombia. Métodos: Se realizó un estudio observacional, descriptivo, de corte transversal. Se revisaron retrospectivamente las bases de datos del Instituto Departamental de Salud de Nariño y del Hospital Universitario Departamental de Nariño. Resultados: Para el periodo de estudio hubo 2 514 hospitalizaciones por dengue y 460 por chikungunya. Se identificaron 22 casos de dengue grave, con una muerte para el año 2017. La revisión de expedientes de 1 735 pacientes con información completa reveló que las manifestaciones clínicas más frecuentes para dengue fueron: fiebre (100 por ciento), cefalea (84,6 por ciento) y mialgias (83,7 por ciento), seguidos de artralgias, rash y dolor abdominal. El sexo masculino predominó en los casos de diagnóstico de dengue (56,8 por ciento) y el femenino en el diagnóstico de chikungunya (52,0 por ciento. Tanto dengue como chikungunya fueron más frecuentes en la población mayor de 40 años con el 24,5 por ciento y 27,2 por ciento, respectivamente. Conclusiones: La sintomatología similar y los casos complicados resaltan la necesidad de contar con diagnósticos oportunos y diferenciales y capacitación al personal de salud, apoyados por entidades gubernamentales. Se requiere generar programas de intervención enfocados a edades productivas y en regiones con condiciones medioambientales propicias para el desarrollo de vectores transmisores de enfermedades(AU)
Introduction: More than 390 million dengue cases and 20 000 deaths due to this condition are reported worldwide every year. Chikungunya caused a large epidemic in the Americas with more than two and a half million cases until the year 2017. Objective: Identify the clinical and socio-demographic characteristics of the dengue and chikungunya cases reported for the period 2014-2017 in Nariño, Colombia. Methods: A cross-sectional observational descriptive study was conducted. A retrospective review was carried out of the databases at Nariño Departmental Health Institute and Nariño Departmental University Hospital. Results: During the study period there were 2 514 hospital admissions with dengue and 460 with chikungunya. A total 22 severe dengue cases and one death were identified for the year 2017. Review of the medical records of 1 735 patients with complete information revealed that the most common clinical manifestations of dengue were fever (100 percent), headache (84.6 percent) and myalgia (83.7 percent), followed by arthralgia, rash and abdominal pain. A predominance was found of the male sex in dengue cases (56.8 percent) and of the female sex in chikungunya cases (52.0 percent). Both conditions were more frequent in the population aged over 40 years, with 24.5 percent and 27.2 percent, respectively. Conclusions: The similar symptoms and the presence of complicated cases highlight the need for timely differential diagnosis and the training of the health personnel, with the support of government agencies. It is required to develop intervention programs aimed at working ages and regions with environmental conditions favorable for the spread of disease(AU)
Assuntos
Humanos , Masculino , Feminino , Dengue/diagnóstico , Febre de Chikungunya/diagnóstico , Colômbia/epidemiologiaRESUMO
COVID-19 patients can excrete viable SARS-CoV-2 virus via urine and faeces, which has raised concerns over the possibility of COVID-19 transmission via aerosolized contaminated water or via the faecal-oral route. These concerns are especially exacerbated in many low- and middle-income countries, where untreated sewage is frequently discharged to surface waters. SARS-CoV-2 RNA has been detected in river water (RW) and raw wastewater (WW) samples. However, little is known about SARS-CoV-2 viability in these environmental matrices. Determining the persistence of SARS-CoV-2 in water under different environmental conditions is of great importance for basic assumptions in quantitative microbial risk assessment (QMRA). In this study, the persistence of SARS-CoV-2 was assessed using plaque assays following spiking of RW and WW samples with infectious SARS-CoV-2 that was previously isolated from a COVID-19 patient. These assays were carried out on autoclaved RW and WW samples, filtered (0.22 µm) and unfiltered, at 4 °C and 24 °C. Linear and nonlinear regression models were adjusted to the data. The Weibull regression model achieved the lowest root mean square error (RMSE) and was hence chosen to estimate T90 and T99 (time required for 1 log and 2 log reductions, respectively). SARS-CoV-2 remained viable longer in filtered compared with unfiltered samples. RW and WW showed T90 values of 1.9 and 1.2 day and T99 values of 6.4 and 4.0 days, respectively. When samples were filtered through 0.22 µm pore size membranes, T90 values increased to 3.3 and 1.5 days, and T99 increased to 8.5 and 4.5 days, for RW and WW samples, respectively. Remarkable increases in SARS-CoV-2 persistence were observed in assays at 4 °C, which showed T90 values of 7.7 and 5.5 days, and T99 values of 18.7 and 17.5 days for RW and WW, respectively. These results highlight the variability of SARS-CoV-2 persistence in water and wastewater matrices and can be highly relevant to efforts aimed at quantifying water-related risks, which could be valuable for understanding and controlling the pandemic.
Assuntos
COVID-19 , Águas Residuárias , Humanos , RNA Viral , Rios , SARS-CoV-2 , Temperatura , ÁguaRESUMO
An essential requirement for cells to sustain a high proliferating rate is to be paired with enhanced protein synthesis through the production of ribosomes. For this reason, part of the growth-factor signaling pathways, are devoted to activate ribosome biogenesis. Enhanced production of ribosomes is a hallmark in cancer cells, which is boosted by different mechanisms. Here we report that the nucleolar tumor-protein MageB2, whose expression is associated with cell proliferation, also participates in ribosome biogenesis. Studies carried out in both siRNA-mediated MageB2 silenced cells and CRISPR/CAS9-mediated MageB2 knockout (KO) cells showed that its expression is linked to rRNA transcription increase independently of the cell proliferation status. Mechanistically, MageB2 interacts with phospho-UBF, a protein which causes the recruitment of RNA Pol I pre-initiation complex required for rRNA transcription. In addition, cells expressing MageB2 displays enhanced phospho-UBF occupancy at the rDNA gene promoter. Proteomic studies performed in MageB2 KO cells revealed impairment in ribosomal protein (RPs) content. Functionally, enhancement in rRNA production in MageB2 expressing cells, was directly associated with an increased dynamic in protein synthesis. Altogether our results unveil a novel function for a tumor-expressed protein from the MAGE-I family. Findings reported here suggest that nucleolar MageB2 might play a role in enhancing ribosome biogenesis as part of its repertoire to support cancer cell proliferation.
Assuntos
Antígenos de Neoplasias/metabolismo , Proteínas de Neoplasias/metabolismo , Ribossomos/metabolismo , Antígenos de Neoplasias/fisiologia , Linhagem Celular Tumoral , Nucléolo Celular/metabolismo , Proliferação de Células/genética , DNA Ribossômico/genética , DNA Ribossômico/metabolismo , Células HCT116 , Células HEK293 , Humanos , Proteínas de Neoplasias/fisiologia , Neoplasias/genética , Neoplasias/metabolismo , Proteínas Nucleares/metabolismo , Proteínas Pol1 do Complexo de Iniciação de Transcrição/metabolismo , Regiões Promotoras Genéticas/genética , Biossíntese de Proteínas , Processamento de Proteína Pós-Traducional , Proteômica , RNA Polimerase I/metabolismo , RNA Ribossômico/biossíntese , Ribossomos/genética , Transcrição Gênica/genéticaRESUMO
Introducción: En el campo de la salud pública la tendencia es priorizar el tema de la vigilancia nutricional en la población, a través del monitoreo del comportamiento del estado nutricional. Objetivo: Evaluar la situación nutricional en menores de 18 años del municipio Pasto en el periodo 2014-2016. Métodos: Estudio observacional-descriptivo de la situación nutricional de la población de estudio, reportada en las bases de datos de la Secretaría de Salud Municipal de Pasto-Colombia. Resultados: Se analizaron 158 614 registros, de los cuales 40,82 por ciento fueron de menores de 5 años y 9,18 por ciento en edades entre 5-18 años. Respecto a la desnutrición global se encontró que 18,9 por ciento de los menores fueron diagnosticados en riesgo: 7,2 por ciento con desnutrición global aguda y 0,7 por ciento con desnutrición global severa. Para la desnutrición aguda 10,4 por ciento tuvo diagnóstico de riesgo, 4,8 por ciento desnutrición aguda y 0,8 por ciento desnutrición aguda severa. En la estimación de desnutrición crónica 30,9 por ciento de los niños presentó riesgo de retardo en el crecimiento y 13,05 por ciento retardo en el crecimiento. El 16,7 por ciento de la población tuvo sobrepeso, 4,2 por ciento obesidad, 10,6 por ciento riesgo de delgadez y 2,7 por ciento delgadez. Conclusiones: De acuerdo con la Encuesta Nacional de la Situación Nutricional en Colombia, la desnutrición disminuyó en el país entre los años 2010 a 2015. Sin embargo, aún existe desnutrición en el municipio de Pasto que, junto al aumento de la tasa de sobrepeso y obesidad en niños y adolescentes, representa una situación de malnutrición, que podría verse reflejada en importantes problemas para la salud por la generación de enfermedades crónicas a la que conlleva(AU)
Introduction: In the field of public health, the tendency is to prioritize the issue of nutritional surveillance in the population, through the monitoring of the nutritional state´s behavior. Objective: Assess the nutritional state of children under 18 years old in Pasto municipality in the period 2014-2016. Methods: Observational-descriptive study of the studied population´s nutritional state reported in the databases of the Municipal Health Secretariat of Pasto-Colombia. Results: 158 614 records were analyzed, of which 40.82 percent were children under 5 years and 9.18 percent in ages between 5 and 18 years. Regarding global malnutrition, 18.9 percent of children were diagnosed at risk: 7.2 percent with acute global malnutrition and 0.7 percent with severe global malnutrition. For acute malnutrition, 10.4 percent had a risk diagnosis, 4.8 percent acute malnutrition and 0.8 percent severe acute malnutrition. In the estimate of chronic malnutrition, 30.9 percent of the children presented risk of growth retardation and 13.05 percent growth retardation. 16.7 percent of the population were overweight, 4.2 percent obese, 10.6 percent risk of thinness and 2.7 percent thinness. Conclusions: According to the National Survey of the Nutritional Situation in Colombia, malnutrition decreased in the country between 2010 and 2015. However, there is still malnutrition in Pasto municipality which, together with the increase in the rate of overweight and obesity in children and adolescents, represents a situation of malnutrition, which could be reflected in major health problems from the generation of chronic diseases to which it leads(AU)
Assuntos
Humanos , Masculino , Feminino , Criança , Vigilância Alimentar e Nutricional/métodos , Estado Nutricional/genética , Sobrepeso/epidemiologia , Epidemiologia Descritiva , Colômbia , Estudo Observacional , Obesidade/epidemiologiaRESUMO
Normal-to-tumor cell transition is accompanied by changes in gene expression and signal transduction that turns the balance toward cancer-cell phenotype, eluding by different mechanisms, the response of tumor-suppressor genes. Here, we observed that MageC2, a MAGE-I protein able to regulate the p53 tumor-suppressor, is accumulated upon MEK/ERK MAPK activation. Overexpression of H-RasV12 oncogene causes an increase in MageC2 protein that is prevented by pharmacologic inhibition of MEK. Similarly, decrease in MageC2 protein levels is shown in A375 melanoma cells (which harbor B-RafV600E oncogenic mutation) treated with MEK inhibitors. MageC2 protein levels decrease when p14ARF is expressed, causing an Mdm2-independent upregulation of p53 transactivation. However, MageC2 is refractory to p14ARF-driven downregulation when H-RasV12 is co-expressed. Using MageC2 knockout A375 cells generated by CRISPR/CAS9 technology, we demonstrated the relevance of MageC2 protein in reducing p53 transcriptional activity in cells containing hyperactive MEK/ERK signaling. Furthermore, gene expression analysis performed in cancer-genomic databases, supports the correlation of reduced p53 transcriptional activity and high MageC2 expression, in melanoma cells containing Ras or B-Raf driver mutations. Data presented here suggest that MageC2 can be a functional target of the oncogenic MEK/ERK pathway to regulate p53.
Assuntos
Antígenos de Neoplasias/genética , Antígenos de Neoplasias/metabolismo , Melanoma/metabolismo , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Animais , Antígenos de Neoplasias/química , Linhagem Celular Tumoral , Inibidor p16 de Quinase Dependente de Ciclina/genética , Fibroblastos/citologia , Fibroblastos/metabolismo , Regulação Neoplásica da Expressão Gênica , Técnicas de Inativação de Genes , Células HEK293 , Humanos , Sistema de Sinalização das MAP Quinases , Melanoma/genética , Camundongos , Proteínas de Neoplasias/química , Estabilidade Proteica , Proteínas Proto-Oncogênicas B-raf/genética , Proteínas Proto-Oncogênicas p21(ras)/genética , Proteínas Proto-Oncogênicas p21(ras)/metabolismo , Ativação TranscricionalRESUMO
BACKGROUND: Malaria primarily affects populations living in poor socioeconomic conditions, with limited access to basic services, deteriorating environmental conditions, and barriers to accessing health services. Control programmes are designed without participation from the communities involved, ignoring local knowledge and sociopolitical and cultural dynamics surrounding their main health problems, which implies imposing decontextualized control measures that reduce coverage and the impact of interventions. The objective of this study was to determine the community perception of malaria in the municipality of Olaya Herrera in the Colombian Pacific. METHODS: A 41-question survey on knowledge, attitudes, and practices (KAP) related to malaria, the perception of actions by the Department of Health, and access to the health services network was conducted. RESULTS: A total of 134 adults were surveyed, in whose households a total of 671 people lived. According to the survey data, about 80% of the household members included teenagers and children, out of which 61% had malaria at one time, and for 75.3%, this disease is a persistent problem. In spite of this, 57.2% of people who fell ill due to malaria were never visited by health personnel for a follow up. This population claimed that responsibility for who should prevent the disease is shared between each person and the Department of Health. However, personal actions were focused on using mosquito nets, ignoring other important practices to prevent bites. Despite campaigns by the Department of Health, 11.9% of respondents did not know how malaria was transmitted, and 8.96% thought it was transmitted through water. Also, 43.5% said that the Department of Health did not do any work to control malaria and 16% did not know if any action was taken. CONCLUSIONS: In spite of the knowledge about malaria and the efforts of the Department of Health to prevent it, the community actions do not seem to be consistent with this knowledge, as the number of cases of malaria is still high in the area.
Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Malária/psicologia , Percepção , Adulto , Idoso , Idoso de 80 Anos ou mais , Colômbia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
Resumen Antecedentes: En los últimos cinco años se han registrado en el mundo diversos casos de infecciones por zika y chikungunya simultáneos al incremento de los casos de Síndrome de Guillain-Barré, que han mostrado una relación causal potencial que aún no es del todo clara. Objetivo: Describir las manifestaciones clínicas del Síndrome de Guillain-Barré (SGB) y su relación con las infecciones por zika y chikungunya, de acuerdo a la literatura científica. Metodología: Se utilizaron las bases de datos PubMed y ScienceDirect para llevar a cabo la búsqueda para el periodo 2014-2016 con las palabras clave: zika, chikungunya y Guillain-Barre Syndrome; se incluyeron artículos en inglés y español. Resultados: Se encontraron 35 artículos: uno del 2014, dos del 2015 y 32 del 2016. Conclusión: Las variaciones en las características clínicas y el incremento en la incidencia de SGB ante la presencia de zika y chikungunya, resaltan la necesidad de realizar vigilancia a estas infecciones y de realizar estudios analíticos para determinar la asociación entre los arbovirus con diferentes alteraciones neurológicas.
Abstract Background: In the last five years, there have been several cases of zika and chikungunya infections in the world, simultaneous with the increase in cases of Guillain-Barre syndrome, which have shown a potential causal relationship that is still not entirely clear. Objective: To describe the main clinical manifestations of Guillain-Barre syndrome in relation to chikungunya and zika, according to scientific literature. Methods: The databases PubMed and ScienceDirect were used to perform the search for the period 2014-2016 using with the keywords: zika, chikungunya and Guillain-Barre Syndrome; articles in English and Spanish were included. Results: 35 articles were found, one for the year 2014, two for 2015 and 32 for 2016. Conclusion: Variations in clinical characteristics and the increased incidence of GBS in the presence of zika and chikungunya, highlight the need to monitor these infections and perform analytic studies to determine the association between arboviruses and different neurological alterations.
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OBJECTIVE: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia. METHODS: This was a descriptive study using the database of the Mycobacteriology Laboratory of the Octávio Mangabeira Specialized Hospital, in the city of Salvador, and georeferencing software. We evaluated 3,877 sputum samples collected from symptomatic respiratory patients, under routine conditions, between June of 2014 and March of 2015. All of the samples were submitted to sputum smear microscopy and the Xpert MTB/RIF test. Patients were stratified by gender, age, and geolocation. RESULTS: Among the 3,877 sputum samples evaluated, the Xpert MTB/RIF test detected M. tuberculosis in 678 (17.5%), of which 60 (8.8%) showed resistance to rifampin. The Xpert MTB/RIF test detected M. tuberculosis in 254 patients who tested negative for sputum smear microscopy, thus increasing the diagnostic power by 59.9%. CONCLUSIONS: The use of the Xpert MTB/RIF test, under routine conditions, significantly increased the detection of cases of tuberculosis among sputum smear-negative patients.
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Testes Diagnósticos de Rotina/métodos , Técnicas de Diagnóstico Molecular/métodos , Mycobacterium tuberculosis/isolamento & purificação , Escarro/microbiologia , Tuberculose/diagnóstico , Adulto , Antibióticos Antituberculose/uso terapêutico , Brasil , Farmacorresistência Bacteriana/efeitos dos fármacos , Feminino , Humanos , Masculino , Microscopia/métodos , Pessoa de Meia-Idade , Mycobacterium tuberculosis/efeitos dos fármacos , Valores de Referência , Reprodutibilidade dos Testes , Estudos Retrospectivos , Rifampina/uso terapêutico , Sensibilidade e Especificidade , Centros de Atenção Terciária , Resultado do Tratamento , Tuberculose/tratamento farmacológico , Tuberculose/microbiologiaRESUMO
ABSTRACT Objective: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia. Methods: This was a descriptive study using the database of the Mycobacteriology Laboratory of the Octávio Mangabeira Specialized Hospital, in the city of Salvador, and georeferencing software. We evaluated 3,877 sputum samples collected from symptomatic respiratory patients, under routine conditions, between June of 2014 and March of 2015. All of the samples were submitted to sputum smear microscopy and the Xpert MTB/RIF test. Patients were stratified by gender, age, and geolocation. Results: Among the 3,877 sputum samples evaluated, the Xpert MTB/RIF test detected M. tuberculosis in 678 (17.5%), of which 60 (8.8%) showed resistance to rifampin. The Xpert MTB/RIF test detected M. tuberculosis in 254 patients who tested negative for sputum smear microscopy, thus increasing the diagnostic power by 59.9%. Conclusions: The use of the Xpert MTB/RIF test, under routine conditions, significantly increased the detection of cases of tuberculosis among sputum smear-negative patients.
RESUMO Objetivo: Avaliar o impacto do teste rápido molecular automatizado Xpert MTB/RIF, utilizado para a detecção de Mycobacterium tuberculosis e sua resistência à rifampicina, em condições de rotina, em um hospital de referência no estado da Bahia. Métodos: Estudo descritivo retrospectivo utilizando o banco de dados do Laboratório de Micobacteriologia do Hospital Especializado Octávio Mangabeira, localizado na cidade de Salvador, e um programa de georreferenciamento. Entre junho de 2014 e março de 2015, foram incluídas no estudo 3.877 amostras de escarro coletadas de pacientes sintomáticos respiratórios em condições de rotina. Todas as amostras coletadas foram submetidas tanto à baciloscopia quanto a Xpert MTB/RIF. Os pacientes foram estratificados por sexo, idade e georreferenciamento. Resultados: Das 3.877 amostras de escarro analisadas, Xpert MTB/RIF detectou a presença de M. tuberculosis em 678 pacientes (17,5%). Desses, 60 (8,8%) apresentaram resistência à rifampicina. O Xpert MTB/RIF detectou 254 pacientes com baciloscopia negativa, representando um acréscimo diagnóstico de 59,9%. Conclusões: A implantação do Xpert MTB/RIF, sob condições de rotina, teve um impacto significativo no aumento da detecção de casos de tuberculose em pacientes com baciloscopia negativa.
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Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Escarro/microbiologia , Tuberculose/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Testes Diagnósticos de Rotina/métodos , Mycobacterium tuberculosis/isolamento & purificação , Valores de Referência , Rifampina/uso terapêutico , Tuberculose/microbiologia , Tuberculose/tratamento farmacológico , Brasil , Reprodutibilidade dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade , Resultado do Tratamento , Farmacorresistência Bacteriana/efeitos dos fármacos , Centros de Atenção Terciária , Microscopia/métodos , Antibióticos Antituberculose/uso terapêutico , Mycobacterium tuberculosis/efeitos dos fármacosRESUMO
OBJECTIVE: To determine the diagnostic accuracy of Thwaites Index (TI) in a Colombian population to distinguish meningeal tuberculosis (MTB) from bacterial meningitis (BM) and from non-tuberculous meningitis. Exploratory analyses were conducted to assess the TI's validity for patients with human immunodeficiency virus (HIV) and children above six-years-old. METHODS: The study included 527 patients, the TI was calculated and results compared with those of a reference standard established by expert neurologists. Sensitivity, specificity, area under the curve of receiver-operator characteristics (AUC-ROC) and likelihood ratios were calculated. RESULTS: The AUC-ROC to distinguish MTB from non-tuberculous meningitis was 0.72 (95% CI: 0.67-0.77) for HIV negative adults. AUC-ROC was 0.62 (95% CI: 0.50-0.74) for HIV positive adults and 0.83 (95% CI: 0.68-0.97) for children. For distinguishing MTB from BM the AUC-ROC was 0.78 (95% CI: 0.73-0.83); furthermore, the AUC-ROC was 0.57 (95% CI: 0.31-0.83) for HIV positive adults and 0.86 (95% CI: 0.73-0.99) for children. CONCLUSION: The TI was sensitive but not specific when used to distinguish MTB from BM in HIV negative adults. In HIV positive adults the index had low diagnostic accuracy. Moreover, the TI showed discrimination capability for children over 6years; however, research with larger samples is required in these.
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Tuberculose Meníngea/líquido cefalorraquidiano , Tuberculose Meníngea/diagnóstico , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Área Sob a Curva , Biomarcadores/líquido cefalorraquidiano , Criança , Colômbia , Estudos Transversais , Diagnóstico Diferencial , Feminino , Infecções por HIV/líquido cefalorraquidiano , Infecções por HIV/complicações , Humanos , Funções Verossimilhança , Masculino , Pessoa de Meia-Idade , Curva ROC , Tuberculose Meníngea/complicações , Adulto JovemRESUMO
Introducción: Los insectos flebotomíneos del grupo Verrucarum, son vectores de interés en salud pública dada su participación en la transmisión de las enfermedades tropicales: leishmaniasis y bartonelosis. El empleo de marcadores moleculares como herramienta de identificación y análisis genético de especies que se comportan como vectores potenciales y confirmados, muestran un uso ampliamente difundido, que genera un conjunto de información que se actualiza constantemente, más aun cuando la identificación de estas especies, es prioritaria en los países donde estas enfermedades ocurren de forma recurrente. Objetivo: Se presenta una revisión sistemática con el objetivo de recopilar y presentar una actualización sobre el uso de marcadores moleculares empleados en especies del grupo Verrucarum. Materiales y métodos: Esta búsqueda se efectuó en un rango de literatura relevante publicada en un periodo de 24 años que incluyó 23 artículos en la temática de genética y biología molecular de flebótomos y 39 artículos de otras áreas como sistemática, ecología de flebótomos y microbiología de agentes patógenos asociados. Resultados: Los resultados muestran el empleo de los genes que codifica para el ARN ribosomal 18S y para el citocromo oxidasa subunidad I como los más efectivos para observar las relaciones filogenéticas entre especies del grupo Verrucarum; el uso del gen citocromo b como carácter taxonómico alternativo para identificar correctamente los flebótomos y, el uso de secuencias espaciadoras de la transcripción interna del ARN ribosomal y el gen citocromo b para determinar la variación genética intra e interespecífica de las poblaciones. Conclusión: Esta revisión contribuirá a estudios filogenéticos de vectores de importancia en salud pública.
Introduction: The phlebotomine flies of the Verrucarum group are vectors of interest in public health because of their participation in the transmission of the tropical diseases: leishmaniasis and bartonellosis. The use of molecular markers, as a tool for the identification and genetic analysis of species that behave as potential and confirmed vectors, show a widely disseminated use that generates a set of information that is constantly updated, even more when the identification of these species is a priority in the countries where these diseases occur on a recurring basis. Objective: The paper presents a systematic review that aims at compiling and presenting an update on the use of molecular markers employed in species of the Verrucarum group. Materials and methods: This search was conducted in a range of relevant literature published in a period of 24 years which included 23 articles on the subject of genetics and molecular biology of sand flies, and 39 articles from other areas such as systematic, ecology of sand flies and microbiology of related pathogenic agents. Results: The results show the use of genes coding for ribosomal 18S RNA and cytochrome oxidase subunit I as the most effective to observe the phylogenetic relationships among species of the Verrucarum group; the use of the cytochrome b gene as alternative taxonomic character to correctly identify the sand flies, and the use of spacer sequences of the internal transcript of the ribosomal RNA and the Cytochrome b gene to determine the genetic variation of intra- and inter-specific populations. Conclusions: This review will contribute to phylogenetic studies of vectors of public health importance.
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Psychodidae , Vetores de Doenças , Filogeografia , Genética Populacional , Marcadores GenéticosRESUMO
Febrile seizures and epilepsy are believed to be linked and some forms of epilepsy are associated with a history of febrile seizures (FS). Linkage analysis to seven known loci for FS and/or genetic epilepsy with febrile seizures plus (GEFS plus) was performed in a small Colombian family. Short tandem repeat (STR) markers were genotyped and two-point linkage analysis and haplotype reconstruction were conducted. A maximum LOD score of 0.75 at marker D8S533 for FEB1 at a recombination fraction (θ) of 0 and a segregating haplotype were identified. FEB1 was the first locus to be associated with FS and this is the second report to describe this association. Two genes in this region, CRH and DEPDC2, are good putative candidate genes that may play a role in FS and/or GEFS plus.