RESUMO
We describe the dynamics of Zika virus (ZIKV) infection in a man in his early 40s who developed fever and rash after returning from Haiti to Italy, in January 2016. Follow-up laboratory testing demonstrated detectable ZIKV RNA in plasma up to day 9 after symptom onset and in urine and saliva up to days 15 and 47, respectively. Notably, persistent shedding of ZIKV RNA was demonstrated in semen, still detectable at 181 days after onset.
Assuntos
Febre/virologia , RNA Viral/sangue , Saliva/virologia , Sêmen/química , Eliminação de Partículas Virais , Infecção por Zika virus/diagnóstico , Zika virus/isolamento & purificação , Adulto , Anticorpos Antivirais/sangue , Exantema/virologia , Haiti , Humanos , Imunoglobulina M/sangue , Itália , RNA Viral/genética , RNA Viral/urina , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sêmen/virologia , Análise do Sêmen , Análise de Sequência de RNA , Viagem , Urina/virologia , Carga Viral , Zika virus/genética , Infecção por Zika virus/sangue , Infecção por Zika virus/virologiaRESUMO
We report the isolation of infectious Zika virus (ZIKV) in cell culture from the saliva of a patient who developed a febrile illness after returning from the Dominican Republic to Italy, in January 2016. The patient had prolonged shedding of viral RNA in saliva and urine, at higher load than in blood, for up to 29 days after symptom onset. Sequencing of ZIKV genome showed relatedness with strains from Latin America.