RESUMO
Most Brazilian Yersinia pestis isolates display a typical plasmid profile composed of the three classical plasmids: pYV, pPst and pFra. However, some cultures lack at least one of these plasmids, while a few of them harbour atypical DNA bands of molecular weight ranging from 147 to 11.5 kb. To investigate whether Y. pestis displaying atypical plasmid content could be propagated among rodents in nature through flea bites, we carried out studies with fleas ( Xenopsylla cheopis) and rodents ( Calomys callosus) reared in the laboratory and five Y. pestis cultures differing in plasmid content. The results suggest that: (1) the single presence of pYV is not sufficient for the transmission of Y. pestis by fleas, (2) pPst is not essential for transmission, (3) two atypical DNA bands of molecular weight of 30 kb and >90 kb have no biological role, and (4) pFra is required for the transmission of Y. pestis by flea bites. Other studies are needed to determine whether this plasmid alone is sufficient for transmission.
Assuntos
Muridae/microbiologia , Peste/transmissão , Plasmídeos/genética , Sifonápteros/microbiologia , Yersinia pestis/genética , Animais , Meios de Cultura , Interações Hospedeiro-Parasita , Camundongos , Plasmídeos/fisiologia , Yersinia pestis/crescimento & desenvolvimentoRESUMO
Os autores tecem comentarios a respeito da necessidade de uma sistematizacao no atendimento extra-hospitalar do politraumatizado e, em particular, do traumatizado de torax e do abdome. Enfantizam a necessidade de um melhor treinamento para todos aqueles que se propoem a esse tipo de atendimento, alem das melhorias das ambulancias no tocante a seus recursos terapeuticos disponiveis.Chamam tambem atencao quanto a importancia da comunicacao, em tadas as fases do atendimento, do medico com o setor de emergencia