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Since their discovery in 2004, carbon dots (CDs) have attracted attention due to their intrinsic physicochemical properties and the easy synthesis from simple precursors. However, quantification of CDs in mixtures of nanoparticles with similar sizes and surface functionality is still a challenging issue for research applications or regulatory purposes. In this work, CDs and silver nanoparticles were first synthesized under alkaline conditions by using glucose as precursor and capping agent, respectively. Mixtures of these nanoparticles were made at micromolar range, without purification, and then analyzed by CE-DAD, using an electrolyte solution composed of 20 mM sodium borate and 20 mM SDS at pH 8.5, in a total time of <15 min. The three-way electrophoretic data were then decomposed by advanced chemometric models, parallel factor analysis and multivariate curve resolution-alternating least-squares. The explained variances for both models were 95.8% (parallel factor analysis) and 85.3% (multivariate curve resolution-alternating least-squares). In both cases, the quality of the results was verified by the root mean square standard deviation coefficient variation, which resulted lower than 5%, and no significant bias was observed at 95% of statistical confidence. Satisfactory prediction for CDs concentration was obtained with recovery values between 80.0% and 115%.
Assuntos
Carbono , Nanopartículas Metálicas , Carbono/química , Quimiometria , Eletroforese Capilar/métodos , PrataRESUMO
Melamine is a compound commonly used in the manufacturing of plastic and flame retardant products, but due to its solubility on water and high nitrogen content, it is also used to adulterate milk to mask adulteration by dilution in protein content tests. This work proposes a quick method using excitation-emission matrix (EEM) fluorescence spectroscopy and second-order calibration methods (PARAFAC and U-PLS/RBL) for the identification and quantification of melamine in milk. The proposed method uses a single clean-up step with acetic acid, resulting in a quick, low-cost, and environmentally friendly procedure, in agreement with green chemistry principles. Both PARAFAC and U-PLS/RBL were capable of detecting melamine in milk above 120.6 and 146.5 ppm respectively, adequate for adulterations above 2% in volume, with RMSEPs of 68.6 and 81.9 ppm, respectively.
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Leite , Triazinas , Animais , Calibragem , Contaminação de Alimentos/análise , Leite/química , Espectrometria de Fluorescência , Triazinas/análiseRESUMO
Between November 2019 and February 2020, 53 water samples were collected along 430 km of coastline in northeastern Brazil, which was the location of an oil spill that occurred in August 2019. Synchronous fluorescence matrices (SFMs) were acquired to avoid regions affected by Raman Stokes scatterings and second harmonic signals, and then, the SFMs were converted into excitation-emission matrices (EEM) by shear transformation. The matrix coupled with parallel factor analysis (PARAFAC) was used in the study of fluorescent components present in the collected waters. A sample collected before the oil spill and another from Florianópolis-SC, 2000 km from the incident, were used as references for nonimpacted waters. In the postspill samples, 4 components were determined, with component 1 (λexc = 225 nm, λem = 475 nm) being associated with humic-like organic matter (terrestrial), component 2 (λexc = 230 nm, λem = 390 nm) being associated with humic-like organic matter (marine), component 3 (λexc = 225/295 nm, λem = 345 nm) being associated with dibenzothiophene-like components also observed in tests with crude oil samples, and component 4 (λexc = 220/280 nm, λem = 340 nm) being associated with a naphthalene-like substance. Principal component analysis (PCA) was performed on the PARAFAC scores. The distribution of samples along the 4 components was observed and compared with the reference samples.
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Poluição por Petróleo , Poluentes Químicos da Água , Brasil , Análise Fatorial , Substâncias Húmicas/análise , Poluição por Petróleo/análise , Espectrometria de Fluorescência , Poluentes Químicos da Água/análiseRESUMO
This work investigated the application of partial least-squares regression of complex numbers on multivariate data obtained by electrochemical impedance spectroscopy (EIS). The use of complex numbers-PLS was evaluated in the individual determination of two well-known redox probes: ferrocyanide and hydroquinone. The predictive ability of complex numbers-PLS was evaluated for EIS spectra obtained at different applied potentials and perturbation amplitudes, and was also compared to that obtained with PLS applied to EIS data presented as real numbers - only the real or imaginary part of the complex impedance, or the absolute impedance or the phase angle. It is shown that complex numbers-PLS is more efficient (better prediction models) when more complex electrochemical systems (hydroquinone) are probed. Excellent predictions were obtained for the determination of hydroquinone and catechol in the direct analysis of spiked tap water samples with EIS and complex numbers-PLS.
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The quantification of zinc in over-the-counter drugs as commercial propolis extracts by molecular fluorescence technique using meso-tetrakis(4-carboxyphenyl)porphyrin (H2 TCPP4 ) was developed for the first time. The calibration curve is linear from 6.60 to 100 nmol L-1 of Zn2+ . The detection and quantification limits were 6.22 nmol L-1 and 19.0 nmol L-1 , respectively. The reproducibility and repeatability calculated as the percentage variation of slopes of seven calibration curves were 6.75% and 4.61%, respectively. Commercial propolis extract samples from four Brazilian states were analyzed and the results (0.329-0.797 mg/100 mL) obtained with this method are in good agreement with that obtained with the Atomic Absorption Spectroscopy (AAS) technique. The method is simple, fast, of low cost and allows the analysis of the samples without pretreatment. Moreover the major advantage is that Zn-porphyrin complex presents fluorescent characteristic promoting the selectivity and sensitivity of the method.
Assuntos
Própole/análise , Espectrometria de Fluorescência/métodos , Zinco/análise , Brasil , Calibragem , Corantes Fluorescentes/química , Mesoporfirinas/química , Porfirinas/química , Própole/química , Reprodutibilidade dos Testes , Espectrofotometria Atômica/métodos , Espectrofotometria UltravioletaRESUMO
Generalized analytical sensitivity (γ) is proposed as a new figure of merit, which can be estimated from a multivariate calibration data set. It can be confidently applied to compare different calibration methodologies, and helps to solve literature inconsistencies on the relationship between classical sensitivity and prediction error. In contrast to the classical plain sensitivity, γ incorporates the noise properties in its definition, and its inverse is well correlated with root mean square errors of prediction in the presence of general noise structures. The proposal is supported by studying simulated and experimental first-order multivariate calibration systems with various models, namely multiple linear regression, principal component regression (PCR) and maximum likelihood PCR (MLPCR). The simulations included instrumental noise of different types: independently and identically distributed (iid), correlated (pink) and proportional noise, while the experimental data carried noise which is clearly non-iid.
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In this work, we tested the PM6, PM6-DH+, PM6-D3, and PM7 enthalpies of formation in aqueous solution as scoring functions across 33 decoy sets to discriminate native structures or good models in a decoy set. In each set these semiempirical quantum chemistry methods were compared according to enthalpic and geometric criteria. Enthalpically, we compared the methods according to how much lower was the enthalpy of each native, when compared with the mean enthalpy of its set. Geometrically, we compared the methods according to the fraction of native contacts (Q), which is a measure of geometric closeness between an arbitrary structure and the native. For each set and method, the Q of the best decoy was compared with the Q0 , which is the Q of the decoy closest to the native in the set. It was shown that the PM7 method is able to assign larger energy differences between the native structure and the decoys in a set, arguably because of a better description of dispersion interactions, however PM6-DH+ was slightly better than the rest at selecting geometrically good models in the absence of a native structure in the set. © 2016 Wiley Periodicals, Inc.
Assuntos
Proteínas/química , Teoria Quântica , Termodinâmica , Conformação Proteica , SoluçõesRESUMO
The obi (Cola acuminate) is a native fruit from Africa, which has been mainly used in the production of soft drinks and also in rituals of African religions. In this paper, the mineral composition of obi collected in seven different cities from Bahia State, Brazil was determined and evaluated using multivariate analysis. The samples were digested using nitric acid and hydrogen peroxide and were analyzed using inductively coupled plasma optical emission spectrometry. The accuracy of the method was confirmed by analysis of a certified reference material of apple leaves, furnished by National Institute of Standard and Technology. The study involved 46 samples, being 18 of the red specie and 28 for the white specie. The results expressed as milligrams of element per 100 g(-1) of sample demonstrated that the concentration ranges varied of 21.28-548.77 for potassium, 15.73-129.85 for phosphorous, 27.95-286.92 for calcium, 7.67-134.45 for magnesium, 0.05-1.41 for manganese, 0.21-0.94 for iron, 0.11-0.39 for copper, 0.27-1.35 for zinc, and 0.025-0.517 for strontium. The principal component analysis and hierarchical cluster analysis evidenced that the mineral composition of the red specie is different of the white specie. The red obi has mineral content higher than white obi.
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Cola/química , Minerais/análise , Cálcio/análise , Cobre/análise , Magnésio/análise , Manganês/análise , Fósforo/análise , Potássio/análise , Zinco/análiseRESUMO
In this study, a novel, simple, and efficient spectrofluorimetric method to determine directly and simultaneously five phenolic compounds (hydroquinone, resorcinol, phenol, m-cresol and p-cresol) in air samples is presented. For this purpose, variable selection by the successive projections algorithm (SPA) is used in order to obtain simple multiple linear regression (MLR) models based on a small subset of wavelengths. For comparison, partial least square (PLS) regression is also employed in full-spectrum. The concentrations of the calibration matrix ranged from 0.02 to 0.2 mg L(-1) for hydroquinone, from 0.05 to 0.6 mg L(-1) for resorcinol, and from 0.05 to 0.4 mg L(-1) for phenol, m-cresol and p-cresol; incidentally, such ranges are in accordance with the Argentinean environmental legislation. To verify the accuracy of the proposed method a recovery study on real air samples of smoking environment was carried out with satisfactory results (94-104%). The advantage of the proposed method is that it requires only spectrofluorimetric measurements of samples and chemometric modeling for simultaneous determination of five phenols. With it, air is simply sampled and no pre-treatment sample is needed (i.e., separation steps and derivatization reagents are avoided) that means a great saving of time.
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Cresóis/análise , Hidroquinonas/análise , Fenol/análise , Resorcinóis/análise , Ar , Poluentes Atmosféricos , Calibragem , Humanos , Análise dos Mínimos Quadrados , Análise de Regressão , Fumaça/análise , Fumar , Espectrometria de Fluorescência/métodosRESUMO
An enzymatic flow-batch system with spectrophotometric detection was developed for simultaneous determination of levodopa [(S)-2 amino-3-(3,4-dihydroxyphenyl)propionic acid] and carbidopa [(S)-3-(3,4-dihydroxyphenyl)-2-hydrazino-2-methylpropionic acid] in pharmaceutical preparations. The data were analysed by univariate method, partial least squares (PLS) and a novel variable selection for multiple lineal regression (MLR), the successive projections algorithm (SPA). The enzyme polyphenol oxidase (PPO; EC 1.14.18.1) obtained from Ipomoea batatas (L.) Lam. was used to oxidize both analytes to their respective dopaquinones, which presented a strong absorption between 295 and 540 nm. The statistical parameters (RMSE and correlation coefficient) calculated after the PLS in the spectral region between 295 and 540 nm and MLR-SPA application were appropriate for levodopa and carbidopa. A comparative study of univariate, PLS, in different ranges, and MLR-SPA chemometrics models, was carried out by applying the elliptical joint confidence region (EJCR) test. The results were satisfactory for PLS in the spectral region between 295 and 540 nm and for MLR-SPA. Tablets of commercial samples were analysed and the results obtained are in close agreement with both, spectrophotometric and HPLC pharmacopeia methods. The sample throughput was 18 h(-1).
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Algoritmos , Carbidopa/análise , Catecol Oxidase/química , Ipomoea batatas/enzimologia , Levodopa/análise , Preparações Farmacêuticas/química , Espectrofotometria/métodos , Soluções Tampão , Carbidopa/química , Catecol Oxidase/metabolismo , Simulação por Computador , Análise dos Mínimos Quadrados , Levodopa/química , Modelos Lineares , Oxirredução , Fosfatos/química , Fatores de TempoRESUMO
This paper proposes a method for determination of chemical oxygen demand (COD) in domestic wastewater. The proposed method is based on near-infrared reflectance (NIRR) measurements of seston collected from wastewater samples by filtration. The analysis does not require any special reagent, catalyst or solvent. Inherent baseline and noise features present in NIRR spectra are removed by a Savitzky-Golay derivative procedure followed by wavelet denoising. The resulting wavelet approximation coefficients are used for partial-least-squares modelling and subsequent prediction of COD values in new samples. The model is calibrated by using COD values obtained according to the American Public Health Association (APHA) reference method. The proposed method is applied to effluent samples from the anaerobic ponds of the Mangabeira municipal wastewater treatment plant in the city of João Pessoa (Paraíba, Brazil). By comparing the NIRR prediction results with the APHA reference values, a root-mean-square error of prediction (RMSEP) of 19 mg O2 L(-1) and a correlation of 0.97 were obtained. Such results are deemed adequate in view of the joint estimate of the standard error of the reference method, which was calculated as 21 mg O2 L(-1).