Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
J Virol Methods ; 256: 24-31, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29496429

RESUMO

Group C Rotavirus (RVC) has been associated globally with sporadic outbreaks of gastroenteritis in children and adults. RVC also infects animals, and interspecies transmission has been reported as well as its zoonotic potential. Considering its genetic diversity and the absence of effective vaccines, it is important and necessary to develop new generation vaccines against RVC for both humans and animals. The aim of the present study was to develop and characterize an HSV-1-based amplicon vector expressing a human RVC-VP6 protein and evaluate the humoral immune response induced after immunizing BALB/c mice. Local fecal samples positive for RVC were used for isolation and sequencing of the vp6 gene, which phylogenetically belongs to the I2 genotype. We show here that cells infected with the HSV[VP6C] amplicon vector efficiently express the VP6 protein, and induced specific anti-RVC antibodies in mice immunized with HSV[VP6C], in a prime-boost schedule. This work highlights that amplicon vectors are an attractive platform for the generation of safe genetic immunogens against RVC, without the addition of external adjuvants.


Assuntos
Antígenos Virais/genética , Antígenos Virais/imunologia , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/imunologia , Expressão Gênica , Vetores Genéticos/genética , Herpesvirus Humano 1/genética , Rotavirus/genética , Rotavirus/imunologia , Animais , Anticorpos Antivirais/imunologia , Chlorocebus aethiops , Humanos , Imunidade Humoral , Masculino , Camundongos , Filogenia , Proteínas Recombinantes , Células Vero
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA