RESUMO
The South American fruit fly Anastrepha fraterculus (Wiedemann) is a complex of cryptic species, the so-called "Anastrepha fraterculus complex", for which eight morphotypes are currently recognized. A previous analysis of ITS1 in samples of the Anastrepha fraterculus complex, while revealing high distinctiveness among samples from different localities of South America, Central America, and Mexico, no direct association was made between sequence type and morphotype. In the present report, a correlated analysis of morphometry and ITS1 data involved individuals belonging to the same population samples. Although showing a low level of intra-populational nucleotide variability, the ITS1 analysis indicated numerous inter-population sequence type variants. Morphotypes identified by morphometric analysis based on female wing shape were highly concordant with ITS1 genetic data. The correlation of genetic divergence and morphological differences among the tested samples gives strong evidence of a robust dataset, thereby indicating the existence of various taxonomic species within the A. fraterculus complex. However, the data revealed genetic and morphological variations in some regions, suggesting that further analysis is still required for some geographic regions.
RESUMO
Rachiptera limbata Bigot develops on Baccharis linearis (R. et Pav.) in the areas around Santiago, Chile. The larvae feed on stem tissues and secrete a liquid that hardens to form a protective feeding and pupation chamber. The immature stages of Neotropical species of Tephritinae are poorly known. In this paper, the morphology of the immature stages of R. limbata are described and compared, in a phylogenetic context, with other Tephritinae species. Antennomaxillary complex, pads, oral ridge, cephalopharyngeal skeleton, anterior and posterior spiracles and anal lobes of first-, second-, third-instar larvae and pupae were studied with optical and scanning electron microscopy. The cephalopharyngeal skeleton is darkly sclerotized and shows an opening or window in the ventral cornua. This trait seems to be plesiomorphic in R. limbata and in other Neotropical species. First-instar larvae anterior spiracles are absent; whereas in second and third instars spiracles are developed as a row of five short tubules. In first- and second-instar larvae, the posterior spiracular slit has only a single hair per bundle; whereas third-instar larvae lack hairs. This last trait seems to be consequence of a larval development delay and an apomorphic trait in R. limbata, compared to other Neotropical and Neartical species.
Assuntos
Asteraceae/parasitologia , Tephritidae/crescimento & desenvolvimento , Tephritidae/fisiologia , Animais , Larva , Microscopia Eletrônica de Varredura , Tephritidae/anatomia & histologiaRESUMO
Rachiptera limbata Bigot desenvolve-se em Baccharis linearis (R. et Pav) na região de Santiago, Chile. As larvas alimentam-se dos caules e secretam um líquido, que em contato com o ar, forma uma câmara larval, onde ocorre a passagem para o estádio de pupa. Os estádios larvais das espécies neotropicais de Tephritinae são pouco conhecidos. O presente estudo descreve a morfologia dos estádios larvais de R. limbata, comparando-os com outras espécies de Tephritinae, em uma abordagem filogenética. Foram analisados, em microsopia de luz e de varredura, o complexo antenomaxilar, as dobras da cutícula, as margens da abertura oral, o esqueleto cefalofaríngeo, os espiráculos anterior e posterior e os lóbulos anais das larvas de primeiro, segundo, terceiro estádios e da pupa. O esqueleto cefalofaríngeo é altamente esclerotizado e apresenta um orifício ou abertura na cornua ventral, característica essa que parece ser plesiomórfica em R. limbata e outras espécies neotropicais. Os espiráculos anteriores estão ausentes nas larvas de primeiro estádio. Nas larvas de segundo e terceiro estádios são desenvolvida formando cinco túbulos curtos dispostos em fileira única. Nas larvas de primeiro e segundo estádios, as aberturas dos espiráculos têm cerdas únicas; nas larvas de terceiro estádio não há cerdas. Essa última característica parece decorrer de uma atraso no desenvolvimento (neotenia) da larva de R. limbata, quando comparada a outras espécies neotropicais e neárticas.
Rachiptera limbata Bigot develops on Baccharis linearis (R. et Pav.) in the areas around Santiago, Chile. The larvae feed on stem tissues and secrete a liquid that hardens to form a protective feeding and pupation chamber. The immature stages of Neotropical species of Tephritinae are poorly known. In this paper, the morphology of the immature stages of R. limbata are described and compared, in a phylogenetic context, with other Tephritinae species. Antennomaxillary complex, pads, oral ridge, cephalopharyngeal skeleton, anterior and posterior spiracles and anal lobes of first-, second-, third-instar larvae and pupae were studied with optical and scanning electron microscopy. The cephalopharyngeal skeleton is darkly sclerotized and shows an opening or window in the ventral cornua. This trait seems to be plesiomorphic in R. limbata and in other Neotropical species. First-instar larvae anterior spiracles are absent; whereas in second and third instars spiracles are developed as a row of five short tubules. In first- and second-instar larvae, the posterior spiracular slit has only a single hair per bundle; whereas third-instar larvae lack hairs. This last trait seems to be consequence of a larval development delay and an apomorphic trait in R. limbata, compared to other Neotropical and Neartical species.