RESUMO
The influence of variations in carbon source concentration, cell inocula, pH, presence of other substrates, and other organisms on the biodegradation of 2-chlorophenol (2-CP) was studied for Alcaligenes sp. isolated from natural sources. Assays of biodegradation were performed in batch and continuous-flow fluidized-bed aerobic reactors. Evaluation of biodegradation was performed by determining total phenols, chemical oxygen demand (COD), and 2-CP by ultraviolet (UV) spectrophotometry. Measurement of microbial growth was carried out by the plate count method. Bioassays of acute toxicity were performed to evaluate detoxification by using Daphnia magna. Results obtained show that under batch conditions with initial inocula of 10(6) cells/mL the strain grew exponentially with 100, 200, and 300 mg/L of 2-CP within 48 hr. A lag period was observed with low cell density inocula (10(5) cells/mL). The strain showed marked delay in the biodegradation of 2-CP at pH 5. Removal of target substrate from mixtures containing other carbon sources demonstrated the possibility of concurrent growth. Mineralization of 2-CP was assessed by gas chromatography carried out at the end of the batch assays and at the exit of the continuous-flow reactor. The presence of other organisms (bacteria, rotifers, ciliate, and algae) that developed in the fluidized-bed reactor did not affect the efficacy of the biodegradation of 2-CP. The removal of 2-CP in the two assayed systems was over 97% in all cases. Toxicity was not detected at the exit of the continuous reactor.
Assuntos
Alcaligenes/fisiologia , Clorofenóis/metabolismo , Animais , Biodegradação Ambiental , Bioensaio , Reatores Biológicos , Daphnia , Concentração de Íons de Hidrogênio , Oxigênio/metabolismo , Fenóis/análise , Dinâmica Populacional , Testes de ToxicidadeRESUMO
Se describen los resultados obtenidos en ensayos de biodegradación de 2-CP, empleando reactores "batch" y de lecho fluidizado (LF), operados aerobiamente y con microorganismos autóctonos especificamente seleccionados y optimizados
Assuntos
Biodegradação Ambiental , Leito FluidificadoRESUMO
Se describen los resultados obtenidos en ensayos de biodegradación de 2-CP, empleando reactores "batch" y de lecho fluidizado (LF), operados aerobiamente y con microorganismos autóctonos especificamente seleccionados y optimizados
Assuntos
Biodegradação Ambiental , Leito FluidificadoRESUMO
Los objetivos del presente trabajo fueron: Seleccionar e identificar microorganismos capaces de biodegradar 2-clorofenol en distintos procesos aeróbios; Determinar la cinética y la velocidad de degradación a escala laboratorio empleando reactores batch; Establecer la efectividad de un reactor de lecho fluidizado aeróbico relleno con carbón activado granular para remover 2-CP como única fuente de carbono, suministrando como nutrientes un fertilizante foliar agrícola de uso comercial; Evaluar la remoción de toxicidad a través de bioensayos
Assuntos
Toxicidade , CongressoRESUMO
Ozone and chlorine are agents that disinfect by destroying, neutralizing or inhibiting the growth of pathogenic microorganisms. The treatment of drinking water with ozone has shown to be more efficient against spores of Bacillus subtilis. It was observed that the ozone already in dose of 0.35 mg/l produced the reduction of at least 5 log in populations of approximately 1 x 10(6) cells/ml of Escherichia coli, Vibrio cholerae, Salmonella typhi, Yersinia enterocolitica, Pseudomonas aeruginosa, Aeromonas hydrophila, Listeria monocytogenes and Staphylococcus aureus. With a dose of 0.50 mg/l of chlorine, the reduction was much smaller for the tested microorganisms (except Vibrio cholerae), while the effect of 2 mg/l of chlorine was similar to the ozone treatment. For spores of Bacillus subtilis, the reduction observed with ozone concentrations of 0.35 and 0.70 mg/l was of almost 3 log, while no considerable effect was obtained with chlorine in the tested conditions. Our results have shown that both disinfectans were consumed during the treatment period, probably because of the own water demand and the added bacterial mass.
Assuntos
Bactérias/efeitos dos fármacos , Desinfetantes/farmacologia , Ozônio/farmacologia , Hipoclorito de Sódio/farmacologia , Microbiologia da Água , Enterobacteriaceae/efeitos dos fármacos , Listeria monocytogenes/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Tiossulfatos/farmacologia , Vibrionaceae/efeitos dos fármacosRESUMO
Ozone and chlorine are agents that disinfect by destroying, neutralizing or inhibiting the growth of pathogenic microorganisms. The treatment of drinking water with ozone has shown to be more efficient against spores of Bacillus subtilis. It was observed that the ozone already in dose of 0.35 mg/l produced the reduction of at least 5 log in populations of approximately 1 x 10(6) cells/ml of Escherichia coli, Vibrio cholerae, Salmonella typhi, Yersinia enterocolitica, Pseudomonas aeruginosa, Aeromonas hydrophila, Listeria monocytogenes and Staphylococcus aureus. With a dose of 0.50 mg/l of chlorine, the reduction was much smaller for the tested microorganisms (except Vibrio cholerae), while the effect of 2 mg/l of chlorine was similar to the ozone treatment. For spores of Bacillus subtilis, the reduction observed with ozone concentrations of 0.35 and 0.70 mg/l was of almost 3 log, while no considerable effect was obtained with chlorine in the tested conditions. Our results have shown that both disinfectans were consumed during the treatment period, probably because of the own water demand and the added bacterial mass.
RESUMO
Ozone and chlorine are agents that disinfect by destroying, neutralizing or inhibiting the growth of pathogenic microorganisms. The treatment of drinking water with ozone has shown to be more efficient against spores of Bacillus subtilis. It was observed that the ozone already in dose of 0.35 mg/l produced the reduction of at least 5 log in populations of approximately 1 x 10(6) cells/ml of Escherichia coli, Vibrio cholerae, Salmonella typhi, Yersinia enterocolitica, Pseudomonas aeruginosa, Aeromonas hydrophila, Listeria monocytogenes and Staphylococcus aureus. With a dose of 0.50 mg/l of chlorine, the reduction was much smaller for the tested microorganisms (except Vibrio cholerae), while the effect of 2 mg/l of chlorine was similar to the ozone treatment. For spores of Bacillus subtilis, the reduction observed with ozone concentrations of 0.35 and 0.70 mg/l was of almost 3 log, while no considerable effect was obtained with chlorine in the tested conditions. Our results have shown that both disinfectans were consumed during the treatment period, probably because of the own water demand and the added bacterial mass.