RESUMO
Assisted gene flow (AGF) is a conservation intervention to accelerate species adaptation to climate change by importing genetic diversity into at-risk populations. Corals exemplify both the need for AGF and its technical challenges; corals have declined in abundance, suffered pervasive reproductive failures, and struggled to adapt to climate change, yet mature corals cannot be easily moved for breeding, and coral gametes lose viability within hours. Here, we report the successful demonstration of AGF in corals using cryopreserved sperm that was frozen for 2 to 10 y. We fertilized Acropora palmata eggs from the western Caribbean (Curaçao) with cryopreserved sperm from genetically distinct populations in the eastern and central Caribbean (Florida and Puerto Rico, respectively). We then confirmed interpopulation parentage in the Curaçao-Florida offspring using 19,696 single-nucleotide polymorphism markers. Thus, we provide evidence of reproductive compatibility of a Caribbean coral across a recognized barrier to gene flow. The 6-mo survival of AGF offspring was 42%, the highest ever achieved in this species, yielding the largest wildlife population ever raised from cryopreserved material. By breeding a critically endangered coral across its range without moving adults, we show that AGF using cryopreservation is a viable conservation tool to increase genetic diversity in threatened marine populations.
Assuntos
Antozoários/genética , Fluxo Gênico/genética , Espermatozoides/fisiologia , Animais , Conservação dos Recursos Naturais/métodos , Recifes de Corais , Criopreservação/métodos , Espécies em Perigo de Extinção , Fertilização/genética , Florida , Genética Populacional/métodos , Células Germinativas/fisiologia , Masculino , Porto Rico , Reprodução/genéticaRESUMO
Objetivo: Demostrar el efecto protector del estriol y Glicina max L. (soya) frente al dolor nociceptivo, en ratas ovariectomizadas. Diseño: Estudio experimental. Lugar: Bioterio de la Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Material biológico: Ratas hembras albinas Holtzman de 2,5 a 3 meses de edad. Métodos: Las 29 ratas fueron agrupadas aleatoriamente: grupo 1 (n=8), no ovariectomizadas, tratadas con solución salina (0,1 mL/kg); grupo 2 (n=7), ovariectomizadas y tratadas con solución salina (0,1 mL/kg); grupo 3 (n=7), ovariectomizadas y tratadas con estriol (0,2 mg/kg); grupo 4 (n=7), ovariectomizadas y tratadas con Glycine max L. (60 mg/kg). Se realizó una prueba basal de dolor térmico en el hornillo a todos los animales, así como tres días después del tratamiento farmacológico. Principales medidas de resultados: Se usó el porcentaje de eficiencia antinociceptiva máxima (porcentaje EAM). Resultados: Se encontró que el porcentaje EAM fue mayor en el grupo de ratas no ovariectomizadas (1,6 por ciento) que en el grupo de las ovariectomizadas (0,8 por ciento). Por otro lado, el porcentaje EAM en ratas ovariectomizadas con solución salina (0,8 por ciento) fue menor al compararlo con el grupo de las tratadas con estriol (5,8 por ciento); así también, el porcentaje EAM del grupo de ovariectomizadas tratadas con Glicina max L. (4,2 por ciento) fue superior al grupo de ovariectomizadas tratadas con solución salina (0,8 por ciento), pero esta no fue superior a las del grupo tratadas con estriol. Conclusiones: El estriol y la Glicina max L. ejercieron un papel protector frente al dolor nociceptivo térmico en ratas ovariectomizadas.
Objective: To determine the protective effect of estriol and Glycine max L. (soybean) compared to nociceptive pain in ovariectomized rats. Design: Experimental study. Setting: Faculty of Medicine bioterium, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Holtzman albino female rats 2,5 to 3 months of age. Methods: The 29 rats were grouped randomly: group 1 (n = 8) non ovariectomized rats treated with saline (0.1 mL/kg); group 2 (n = 7) ovariectomized rats treated with saline (0.1 mL / kg); group 3 (n = 7) ovariectomized rats treated with estriol (0.2 mg/kg); group 4 (n = 7) ovariectomized rats treated with Glycine max L. (60 mg/kg). We determined baseline hotplate thermal pain as well as three days after treatment. Main outcome measures: Antinociceptive maximum efficacy (percentage AME). Results: We found that percentage EAM was higher in the non ovariectomized rats group (1.6 per cent) than in the ovariectomized group (0.8 per cent). Furthermore, the percentage of EAM in ovariectomized rats treated with saline (0.8 per cent) compared with the group treated with estriol (5.8 per cent) and also the percentage of EAM in the Glycine max L. group (4.2 per cent) was higher than in the ovariectomized group treated with saline (0.8 per cent) but not higher than the group treated with estriol. Conclusions: Estriol and Glycine max L. have protective role against thermal nociceptive pain in ovariectomized rats.
Assuntos
Animais , Feminino , Ratos , Glicinérgicos , Dor , Estriol , Ovariectomia , Ensaio ClínicoRESUMO
Se realiza la presentación de un caso, portador del síndrome de Reiter en la infancia, considerando el ingreso de un paciente masculino de 12 años de edad de raza blanca, con antecedentes de haber presentado infección gastrointestinal y urogenital. El mismo ingresa en el Hospital Pediátrico Provincial Mártires de las Tunas en el año 2009 en el servicio de miscelánea, por presentar oligoartritis localizada preferentemente en miembros inferiores con manifestaciones extraarticulares, uretritis, conjuntivitis bilateral, dolor abdominal, diarreas; además de manifestaciones cutáneas, que se localizaron en ambos pies, caracterizadas por lesiones de aspecto eritematoso , ulceradas y otras psoriasiformes . Estas manifestaciones se acompañaron de un síndrome febril intermitente. El diagnóstico se confirmó por el antecedente y las características clínicas antes citadas. La presencia del antígeno HLA-B27 es un dato complementario de valor, que no se realizó en el paciente, teniendo en cuenta que el diagnóstico fue seguro, con cultivó del germen responsable de la infección. El laboratorio fue de gran utilidad, y mostró en los diferentes exámenes complementarios eritrosedimentación acelerada, lámina periférica con leucocitosis y trombocitosis. Se ilustraron con fotos las manifestaciones típicas de la enfermedad(AU)
A revision is been carried out about Reiter Syndrome in childhood, considering a male patient aged 12, white, with a past history of having presented gastrointestinal and urogenital infection. He was admitted at the Provincial Pediatric Hospital of Las Tunas in the year 2009, in the miscellanea service, presenting oligoarthritis located basically in the lower limbs with extra- articular manifestations , urethritis, bilateral conjunctivitis, abdominal pain, diarrhea and also with cutaneous manifestations that were located in both feet, characterized by lesions with erythematous aspect, ulcerated and other psoriasis forms. These manifestations were accompanied by an intermittent febrile syndrome. The diagnosis was confirmed by the past history and the clinical characteristics mentioned. The presence of HLA-B27 is a lab test datum of value, that was not carried out in the patient, keeping in mid that the diagnosis was positive with a germ cultivation was responsible for the infection. The laboratory is of great value and the ancillary tests showed that the sedimentation rate was accelerated, peripheral blood studies showed leukocytosis and trombocytosis. The typical manifestation of the illness was illustrated with pictures(AU)