RESUMO
INTRODUCTION: The aim of this study was to evaluate the cell viability and tissue reaction of NeoMTA Plus (NMP; Avalon Biomed Inc, Houston, TX) compared with mineral trioxide aggregate (MTA; Angelus, Londrina, PR, Brazil) and Biodentine (BD; Septodont, Saint-Maur-de-Fossés, France). METHODS: Fibroblasts (3T3) were plated and exposed to 1% extract from the test material before and after setting. Cytotoxicity assessment was performed using the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide and sulforhodamine B assays. In vivo evaluation consisted of polyethylene tube implantation of the materials in rat subcutaneous tissue. Histologic analysis occurred at 7, 30, and 90 days, scoring inflammatory events and collagen fiber formation. Analysis of variance and the Tukey and t tests were used for cytocompatibility assays, and the Kruskal-Wallis test followed by the Dunn test were used for biocompatibility assays (P ≤ .05). RESULTS: The materials in the cytotoxicity assays presented greater viability after setting (P ≤ .05). NMP and MTA presented higher viability than the control (Dulbecco modified Eagle medium) on the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay before and after setting (P ≤ .05). The sulforhodamine B assay showed that MTA and BD presented less viability than NMP and the control, and NMP was similar to the control before setting. After setting, MTA and BD presented higher viability when compared with the control group (P ≤ .05), and NMP was similar to control. Inflammatory infiltrate reduction occurred throughout the test periods for all materials. At 7 days, neutrophils were present in BD (P ≤ .05), and granuloma and giant cells were present in BD and MTA. At 30 days, BD showed intense inflammatory infiltrates and a large number of macrophages when compared with NMP, MTA, and the control (P ≤ .05). At 90 days, BD presented a thick fiber layer compared with NMP (P ≤ .05). CONCLUSIONS: NMP showed similar biocompatible behavior to MTA and BD.
Assuntos
Sobrevivência Celular/efeitos dos fármacos , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Células 3T3 , Compostos de Alumínio/farmacologia , Animais , Compostos de Cálcio/farmacologia , Polpa Dentária/efeitos dos fármacos , Combinação de Medicamentos , Humanos , Técnicas In Vitro , Camundongos , Óxidos/farmacologia , Silicatos/farmacologiaRESUMO
Muitos equipamentos e métodos têm sido empregados para avaliação in vitro do preparo do canal radicular. No entanto, a maioria deles apresenta como limitação a destruição do espécime e a impossibilidade de avaliarsimultaneamente diferentes critérios do preparo do canal radicular. A microtomografia computadorizada (μCT), além ser uma tecnologia não invasiva, permite a visualização tridimensional do canal radicular, oferecendodados reprodutíveis que possibilitam comparações pré e pós- instrumentação. O objetivo do presentetrabalho foi realizar uma análise crítica da μCT, como método de avaliação do preparo do canal radicular.
A variety of devices and methods have been described for the in vitro evaluation of the root canal preparation.However, the majority of these studies has limitations as destruction of the specimens and impedes the simultaneousinvestigation of different parameters of root canal preparation. The microcomputed tomography,non-invasive technique, allows to visualize the morphologic characteristics of the root canal three-dimensionally.Also, the μCT offers reproductive data which possibilities the comparison of the shape of root canalbefore and after preparation. The aim of this study was to perform a critical analysis of the μCT as method toevaluate the root canal preparation by review of the literature.