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1.
Rev Soc Bras Med Trop ; 52: e20190135, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31390442

RESUMO

INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.


Assuntos
Bacillus thuringiensis , Toxinas Bacterianas/farmacologia , Moscas Domésticas/efeitos dos fármacos , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Análise de Variância , Animais , Contagem de Colônia Microbiana , Exotoxinas , Microscopia Eletrônica de Transmissão , Controle Biológico de Vetores/métodos , Reprodutibilidade dos Testes
2.
Rev. Soc. Bras. Med. Trop ; Rev. Soc. Bras. Med. Trop;52: e20190135, 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1041502

RESUMO

Abstract INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.


Assuntos
Animais , Bacillus thuringiensis , Toxinas Bacterianas/farmacologia , Moscas Domésticas/efeitos dos fármacos , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Contagem de Colônia Microbiana , Controle Biológico de Vetores/métodos , Reprodutibilidade dos Testes , Análise de Variância , Microscopia Eletrônica de Transmissão , Exotoxinas
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