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RESUMO: Introdução: A dissecção da artéria carótida (DAC) tem como uma das principais repercussões o Acidente Vascular Cerebral isquêmico (AVCi) em indivíduos jovens previamente saudáveis. É comum que nas DAC traumáticas, como em acidentes automobilísticos, o paciente seja submetido à investigação mais complexa com exames de imagem. Estes permitem um diagnóstico precoce e, portanto, um tratamento, reduzindo as chances de sequelas. Todavia, cau-sas não traumáticas com desfecho de DAC já foram relatadas, como as causas odontológicas. Objetivo: Contribuir para valorização diagnóstica da DAC não traumática e profilaxia de AVC subsequente. Métodos: Relatamos um caso de dissecção não aneurismática das artérias carótidas internas (ACI) pós-procedimento odontológico com desfecho de AVC bilateral em paciente de 52 anos. Resultados: Devido à forte associação de DAC a fatores traumáticos, em um primeiro contato com a paciente não foram levantadas suspeitas da ocorrência de DAC nem de AVC (ausência de déficit focal). Porém, tardiamente, foi identificada oclusão da ACI à esquerda e estenose na ACI direita, resultando em dois AVCs e incapacidade funcional severa na alta. Conclusão: O diagnóstico da DAC é um desafio, em especial por tratar-se de uma lesão de etiologia multifatorial. No entanto, seu reconhecimento precoce afetará diretamente o desfecho do paciente. Portanto, na presença de eventuais sintomas de alerta, faz-se necessário um meticuloso ques-tionamento sobre as últimas atividades do indivíduo. Este estudo alerta para a adoção de condutas profiláticas na ocorrência de fatores causais como hiperextensão cervical prolongada ou movimentos súbitos da cervical. (AU)
ABSTRACT: Introduction: Carotid artery dissection (CAD) has the ischemic stroke as one of the main repercussions in pre-viously healthy young individuals. It is common that in traumatic CAD, as in automobile accidents, the patient is subjected to a more complex investigation with imaging exams. These, allow an early diagnosis and, therefore, a treatment, reducing the chances of sequelae. However, non-traumatic causes with CAD outcomes have already been reported, such as dental causes. Objective: To contribute to the diagnostic valuation of non-traumatic CAD and subsequent stroke prophylaxis. Methods: We report a case of non-aneurysmatic dissection of the internal carotid arteries (ICA) after a dental procedure with evolution to bilateral stroke outcome in a 52-year-old patient. Results: Due to the strong association of CAD with traumatic factors, in a first contact with the patient there was no suspicion of CAD or stroke (absence of focal deficit). However, late, ICA occlusion on the left and stenosis on the right ICA were identified, resulting in two strokes and severe functional disability at discharge. Conclusion:The diagnosis of CAD is a challenge, especially since it is a multifactorial lesion. However, its early recognition will directly affect the patient's outcome. Therefore, in the presence of any warning symptoms, meticulous questioning about the individual's latest activities is necessary. This study alerts to the adoption of prophylactic conducts in the occurrence of causal factors such as prolonged cervical hyperextension or sudden movements of the cervical. (AU)
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Humanos , Feminino , Pessoa de Meia-Idade , Dissecação da Artéria Carótida Interna , Acidente Vascular Cerebral , Implantação Dentária , AVC IsquêmicoRESUMO
In this study, we evaluated the monitoring of tick fever (TF) in a Brazilian dairy farm in the Minas Gerais state, Brazil, from July 10 to August 4, 2018. We aimed to identify diagnostic and treatment flaws in the protocol adopted by the farm, and to establish a novel and accurate TF monitoring protocol based on precision dairy farming and rational use of antimicrobials and antiparasitic drugs, while evaluating the economic benefits of the proposed strategy. We monitored TF in 395 heifer calves aged between 3 and 14 mo. According to the farm's standard protocol, all calves with an increase of 0.5°C in rectal temperature compared with the previous week's measurement were treated for Anaplasma spp. and Babesia spp. Blood smears were collected from the tail tip of the treated calves. During the last week of the study, we prepared blood smears of all calves regardless of treatment indication. Economic analysis was performed. The results indicated that at least 56.86% (261/459) of the calves did not require treatment for TF, whereas only 23.09% (106/459) had treatment indications. Negative blood smears (45.97%; 211/459) indicated the possibility of calves being affected by another disease or a condition that was not being adequately treated or those not necessarily sick. These results demonstrate the excessive use of medications, representing a direct economic loss, in addition to potentially favoring the occurrence of resistance to antimicrobials. In contrast, 9.42% (26/276) of calves had no treatment indication based on rectal temperature but had treatment indications based on blood smears. Only 5.73% (42/735) of blood smears had co-infection with hemopathogens, and none had triple co-infection. Therefore, we proposed the monitoring of TF using rectal temperature and microscopic analysis. If implemented, this strategy would result in a direct annual savings of approximately $22,638.96 (77.99%) related to medication for the treatment of TF. Therefore, implementing the proposed protocol would be cheaper than treatment based only on rectal temperatures. The currently implemented TF protocols overestimate the occurrence of TF, resulting in overtreatment. Thus, implementing a TF monitoring protocol based on a microscopy tool is justified, with benefits including rational use of medication, potential to generate savings, and reduced morbidity and mortality rates, in addition to enabling other diagnoses.
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Babesiose , Doenças dos Bovinos , Carrapatos , Animais , Brasil , Bovinos , Doenças dos Bovinos/tratamento farmacológico , Fazendas , FemininoRESUMO
With a view to reducing stress and discomfort and improving the practicality of making temperature measurements on cats, the aim of this study was to evaluate the use of a tympanic infrared thermometer (TIT), which is an easy-to-use device for measuring temperatures quickly, comfortably and more hygienically. For this, were selected 120 domestic cats living in Sousa, Paraíba, northeastern Brazil. Among them, 100 were normothermic, ten were hypothermic and ten were hyperthermic. None of them had any clinical signs of external or internal otitis. The measurements using the digital thermometer were higher than those using the TIT (P < 0.05), in the normothermic and hyperthermic cats. In the hypothermic cats, there was no difference (P ≥ 0.05) in mean values from the three types of thermometer. The values obtained using the mercury thermometer did not differ (P ≥ 0.05) from the values obtained using the other thermometers. There was a strong positive correlation in almost all the evaluations between the three thermometers, except for digital versus TIT for hyperthermic cats (r < 0.75). Because of the discrepancy between the values obtained by the TIT and the digital rectal thermometer, we suggest that further studies should be carried out in order to establish a temperature correction table for the TIT, so that it can be better used in veterinary medicine.(AU)
Com o objetivo de reduzir o estresse, desconforto e melhorar a praticidade da medição da temperatura em gatos, o objetivo deste estudo foi avaliar o uso de um termômetro timpânico infravermelho (TTI), que é um dispositivo de fácil utilização para medição de temperatura de forma rápida, confortável e higiênica. Para isso, foram selecionados 120 gatos domésticos criados no município de Sousa, Paraíba, Nordeste do Brasil. Dentre eles, 100 eram normotérmicos, dez hipotérmicos e dez hipertérmicos. Nenhum deles apresentava sinais clínicos de otite externa ou interna.(AU)
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Animais , Gatos , Gatos/anatomia & histologia , Gatos/fisiologia , Termômetros/veterinária , Raios InfravermelhosRESUMO
With a view to reducing stress and discomfort and improving the practicality of making temperature measurements on cats, the aim of this study was to evaluate the use of a tympanic infrared thermometer (TIT), which is an easy-to-use device for measuring temperatures quickly, comfortably and more hygienically. For this, were selected 120 domestic cats living in Sousa, Paraíba, northeastern Brazil. Among them, 100 were normothermic, ten were hypothermic and ten were hyperthermic. None of them had any clinical signs of external or internal otitis. The measurements using the digital thermometer were higher than those using the TIT (P < 0.05), in the normothermic and hyperthermic cats. In the hypothermic cats, there was no difference (P ≥ 0.05) in mean values from the three types of thermometer. The values obtained using the mercury thermometer did not differ (P ≥ 0.05) from the values obtained using the other thermometers. There was a strong positive correlation in almost all the evaluations between the three thermometers, except for digital versus TIT for hyperthermic cats (r < 0.75). Because of the discrepancy between the values obtained by the TIT and the digital rectal thermometer, we suggest that further studies should be carried out in order to establish a temperature correction table for the TIT, so that it can be better used in veterinary medicine.
Com o objetivo de reduzir o estresse, desconforto e melhorar a praticidade da medição da temperatura em gatos, o objetivo deste estudo foi avaliar o uso de um termômetro timpânico infravermelho (TTI), que é um dispositivo de fácil utilização para medição de temperatura de forma rápida, confortável e higiênica. Para isso, foram selecionados 120 gatos domésticos criados no município de Sousa, Paraíba, Nordeste do Brasil. Dentre eles, 100 eram normotérmicos, dez hipotérmicos e dez hipertérmicos. Nenhum deles apresentava sinais clínicos de otite externa ou interna.
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Animais , Gatos , Gatos/anatomia & histologia , Gatos/fisiologia , Raios Infravermelhos , Termômetros/veterináriaRESUMO
The COVID-19 pandemic exposes both conflict and cooperation in artisanal and small-scale gold mining in the Brazilian Amazon. Reporting on the experiences of artisanal and small-scale gold miners (garimpeiros, in Brazilian Portuguese), we show how, on the one hand, the pandemic challenges an already precarious working system that could lead garimpeiros, often invisible to public policies, to positions of further vulnerability; and, on the other hand, highlights the capacity of garimpeiros to self-organize and navigate the difficulties by finding alternative solutions to cope with the crisis. This leads us to argue that emerging strategies of cooperation, related to self-organization and communication channels have the potential to provide experiences useful for processes of conflict transformation in the post-crisis. We acknowledge that much depends on the severity of the crisis and its manifestations in the region; nevertheless, the potential for constructive outcomes from the crisis should not be disregarded.
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PURPOSE: To analyze pain scores after surgery in a group of patients submitted to inguinal hernia repair under peripheral nerve block with local or spinal anesthesia. METHODS: Fifty patients were divided into two groups (both with 25 patients each). In the first group the patients were submitted to herniorrhaphy under peripheral block and local anesthesia (LG) and in the other group the patients were submitted to the same procedure under spinal anesthesia (RG). The pain was assessed using the international visual analog pain scale at four different moments. The analysis cost of the procedure was performed using the hospital's average final cost, without including medical expenses. RESULTS: The groups were homogeneous in relation to the epidemiological and clinical features. There was no significant difference between the pain in the intraoperative period and in the return visit for both groups (p = 0.17 and p = 0.18). In the immediate postoperative period, both groups reported no pain at all. In general, the RG reported a greater pain score (16% for RG and 12% for LG). Complications were more frequent in patients submitted to spinal anesthesia (40% versus 8%) (p = 0.008). The surgical time was higher in the LG (39.3 ± 9.2 min) versus (28.7 ± 7.5 min) (p = 0.01). The average final cost of the procedure was US$ 100.98 for the LG and US$ 166.19 for the RG (p = 0.00). CONCLUSION: The inguinal hernioplastia under local anesthesia plus sedation is a safe method, with a low incidence of complications, great acceptance by patients and less expensive.
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Anestesia Local , Raquianestesia , Hérnia Inguinal/cirurgia , Herniorrafia , Bloqueio Nervoso , Dor Pós-Operatória/prevenção & controle , Adulto , Idoso , Sedação Consciente , Feminino , Virilha/cirurgia , Humanos , Período Intraoperatório , Masculino , Pessoa de Meia-Idade , Medição da Dor , Dor Pós-Operatória/diagnóstico , Dor Pós-Operatória/etiologia , Período Pós-OperatórioRESUMO
The effect of HLA-G 14 bp Ins/Del polymorphism (rs371194629) on the risk of preeclampsia has been assessed in several populations, yet the results are still conflicting. Lack of power due to small sample sizes is a common cause of inconsistencies in genetic association studies. We aimed to test whether the maternal polymorphism is associated with preeclampsia, eclampsia or HELLP syndrome (acronym for Hemolysis, Elevation of Liver enzymes, Low Platelets). To achieve a statistical power greater than 0.90, a total of 741 women (332 controls, 246 preeclampsia, 57 eclampsia and 106 HELLP) were genotyped for the 14-bp Ins/Del polymorphism. The genetic association with disease status was assessed by Fisher's exact test and odds ratio (OR) estimates using logistic regression model adjusted for maternal age and parity status. Allele and genotype distributions were the same between control and case groups (p > .05). The polymorphism was not associated with the risk of developing preeclampsia [OR = 0.93 (0.72-1.19); p = .541], or eclampsia [OR = 0.90 (0.60-1.38); p = .628] nor HELLP syndrome [OR = 0.92 (0.66-1.28); p = .628]. This well-powered study clearly demonstrates that the maternal HLA-G 14-bp Ins/Del polymorphism is not associated with preeclampsia risk. However, as the offspring genotypes were not evaluated here, we could not rule out the effect of the foetal genotype on the preeclampsia pathogenesis.
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Pareamento de Bases/genética , Estudos de Associação Genética , Predisposição Genética para Doença , Antígenos HLA-G/genética , Mutação INDEL/genética , Polimorfismo Genético , Pré-Eclâmpsia/genética , Deleção de Sequência/genética , Adulto , Alelos , Feminino , Humanos , Modelos Logísticos , Idade Materna , Fenótipo , Gravidez , Fatores de Risco , Adulto JovemRESUMO
Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167) for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364) for cytopathological examination and bad (κ = -0.041) for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497). The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator.(AU)
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Animais , Cães , Confiabilidade dos Dados , Imuno-Histoquímica/veterinária , Leishmaniose Visceral/patologia , Leishmaniose Visceral/veterinária , Exame de Medula Óssea/veterinária , Reprodutibilidade dos TestesRESUMO
Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167) for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364) for cytopathological examination and bad (κ = -0.041) for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497). The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator.(AU)
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Animais , Cães , Leishmaniose Visceral/patologia , Leishmaniose Visceral/veterinária , Imuno-Histoquímica/veterinária , Confiabilidade dos Dados , Exame de Medula Óssea/veterinária , Reprodutibilidade dos TestesRESUMO
Bacterial ATP-binding cassette (ABC) transporters play a crucial role in the physiology and pathogenicity of different bacterial species. Components of ABC transporters have also been tested as target antigens for the development of vaccines against different bacterial species, such as those belonging to the Streptococcus genus. Streptococcus mutans is the etiological agent of dental caries, and previous studies have demonstrated that deletion of the gene encoding PstS, the substrate-binding component of the phosphate uptake system (Pst), reduced the adherence of the bacteria to abiotic surfaces. In the current study, we generated a recombinant form of the S. mutans PstS protein (rPstS) with preserved structural features, and we evaluated the induction of antibody responses in mice after sublingual mucosal immunization with a formulation containing the recombinant protein and an adjuvant derived from the heat-labile toxin from enterotoxigenic Escherichia coli strains. Mice immunized with rPstS exhibited systemic and secreted antibody responses, measured by the number of immunoglobulin A-secreting cells in draining lymph nodes. Serum antibodies raised in mice immunized with rPstS interfered with the adhesion of bacteria to the oral cavity of naive mice challenged with S. mutans. Similarly, mice actively immunized with rPstS were partially protected from oral colonization after challenge with the S. mutans NG8 strain. Therefore, our results indicate that S. mutans PstS is a potential target antigen capable of inducing specific and protective antibody responses after sublingual administration. Overall, these observations raise interesting perspectives for the development of vaccines to prevent dental caries.
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Anticorpos Antibacterianos/sangue , Cárie Dentária/prevenção & controle , Imunização/métodos , Boca/microbiologia , Proteínas de Ligação a Fosfato/imunologia , Streptococcus mutans/crescimento & desenvolvimento , Streptococcus mutans/imunologia , Adjuvantes Imunológicos , Administração Sublingual , Animais , Antígenos de Bactérias/imunologia , Aderência Bacteriana , Vacinas Bacterianas/imunologia , Cárie Dentária/microbiologia , Escherichia coli Enterotoxigênica/química , Feminino , Imunidade nas Mucosas , Imunoglobulina A/análise , Camundongos , Proteínas de Ligação a Fosfato/administração & dosagem , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/imunologia , Saliva/imunologiaRESUMO
Leptospirosis is a global zoonotic disease caused by different Leptospira species, such as Leptospira interrogans, that colonize the renal tubules of wild and domestic animals. Thus far, attempts to develop effective leptospirosis vaccines, both for humans and animals, have failed to induce immune responses capable of conferring protection and simultaneously preventing renal colonization. In this study, we evaluated the protective immunity induced by subunit vaccines containing seven different recombinant Leptospira interrogans outer membrane proteins, including the carboxy-terminal portion of the immunoglobulinlike protein A (LigA(C)) and six novel antigens, combined with aluminum hydroxide (alum) or Salmonella flagellin (FliC) as adjuvants. Hamsters vaccinated with the different formulations elicited high antigen-specific antibody titers. Immunization with LigA(C), either with alum or flagellin, conferred protective immunity but did not prevent renal colonization. Similarly, animals immunized with LigA(C) or LigA(C) coadministered with six leptospiral proteins with alum adjuvant conferred protection but did not reduce renal colonization. In contrast, immunizing animals with the pool of seven antigens in combination with flagellin conferred protection and significantly reduced renal colonization by the pathogen. The present study emphasizes the relevance of antigen composition and added adjuvant in the efficacy of antileptospirosis subunit vaccines and shows the complex relationship between immune responses and renal colonization by the pathogen.
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Adjuvantes Imunológicos/administração & dosagem , Proteínas da Membrana Bacteriana Externa/imunologia , Vacinas Bacterianas/imunologia , Flagelina/administração & dosagem , Leptospira interrogans/imunologia , Leptospirose/prevenção & controle , Hidróxido de Alumínio/administração & dosagem , Animais , Anticorpos Antibacterianos/sangue , Proteínas da Membrana Bacteriana Externa/genética , Vacinas Bacterianas/administração & dosagem , Vacinas Bacterianas/genética , Imunoglobulina G/sangue , Rim/microbiologia , Leptospira interrogans/genética , Leptospirose/imunologia , Masculino , Mesocricetus , Análise de Sobrevida , Vacinas de Subunidades Antigênicas/administração & dosagem , Vacinas de Subunidades Antigênicas/genética , Vacinas de Subunidades Antigênicas/imunologia , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologiaRESUMO
INTRODUCTION: Preeclampsia is a complex and heterogeneous disease with increased risk of maternal mortality, especially for earlier gestational onset. There is a great inconsistency regarding the genetics of preeclampsia across the literature. The gene Activin A receptor, type IIA (ACVR2A), was reported as associated to preeclampsia in Australian/New Zealand and Norwegian populations. The goal of this study was to validate this genetic association in a Brazilian population. METHODS: We performed a case-control study using 693 controls and 613 cases (443 preeclampsia, 64 eclampsia and 106 HELLP syndrome), from a Northeastern Brazilian population. Five single nucleotide polymorphisms (SNPs) in ACVR2A were tested for association through multiple logistic regression models. RESULTS: There was no statistical association with preeclampsia (per se), eclampsia or HELLP. However, by grouping preeclampsia in accordance to the gestational age at delivery, SNPs rs1424954 (OR = 1.86; 95% CI, 1.25-2.78; p = 0.002) and rs1014064 (OR = 1.77; 95% CI, 1.21-2.60; p = 0.004) were significantly associated with early onset preeclampsia (gestational age ≤ 34 weeks). The risk haplotype had a frequency of 0.468 in early preeclampsia compared to 0.316 in controls (p = 0.0008 and permuted p = 0.002). DISCUSSION: Activin A receptors are important in decidualization, trophoblast invasion and placentation processes during pregnancy. The gene ACVR2A was associated with the more severe early onset preeclampsia. This finding supports the hypothesis of different pathogenic mechanisms contributing to the early- and late-onset preeclampsia.
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Receptores de Activinas Tipo II/genética , Polimorfismo de Nucleotídeo Único , Pré-Eclâmpsia/genética , Adolescente , Adulto , Idade de Início , Brasil/epidemiologia , Estudos de Casos e Controles , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Genótipo , Idade Gestacional , Síndrome HELLP/epidemiologia , Síndrome HELLP/genética , Humanos , Pré-Eclâmpsia/epidemiologia , Gravidez , Adulto JovemRESUMO
Since free-range chickens are important for the epidemiology of toxoplasmosis, this study evaluated the sensitivity and specificity of different laboratory techniques for the diagnosis of Toxoplasma gondii in these animals. Serum samples from 135 adult domestic chickens were tested for anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), modified agglutination test (MAT), and indirect hemagglutination test (IHAT). Tissue samples from all animals were analyzed by histopathology, immunohistochemistry and mouse bioassay (gold standard). Fifty-four chickens were positive for T. gondii in the bioassay. The sensitivity and specificity of the different tests were, respectively, 85% and 56% for ELISA; 80% and 52% for IFAT; 76% and 68% for MAT; 61% and 80% for IHAT; 7% and 98% for immunohistochemistry, and 6% and 98% for histopathology. The MAT was the most effective method for the diagnosis of T. gondii infection in chickens, followed by ELISA. Histopathology and immunohistochemistry are useful tools for the diagnosis of T. gondii infection in chickens due to their specificity.
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Anticorpos Antiprotozoários/sangue , Galinhas/parasitologia , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Testes de Aglutinação/veterinária , Animais , Bioensaio/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Testes de Hemaglutinação/veterinária , Imuno-Histoquímica/veterinária , Camundongos , Sensibilidade e Especificidade , Organismos Livres de Patógenos Específicos , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/parasitologiaRESUMO
The cause of Alzheimer's disease is still unknown, but the disease is distinctively characterized by the accumulation of ß-amyloid plaques and neurofibrillary tangles in the brain. These features have become the primary focus of much of the research looking for new treatments for the disease, including immunotherapy and vaccines targeting ß-amyloid in the brain. Adverse effects observed in a clinical trial based on the ß-amyloid protein were attributed to the presence of the target antigen and emphasized the relevance of finding safer antigen candidates for active immunization. For this kind of approach, different vaccine formulations using DNA, peptide, and heterologous prime-boost immunization regimens have been proposed. Promising results are expected from different vaccine candidates encompassing B-cell epitopes of the ß-amyloid protein. In addition, recent results indicate that targeting another protein involved in the etiology of the disease has opened new perspectives for the effective prevention of the illness. Collectively, the evidence indicates that the idea of finding an effective vaccine for the control of Alzheimer's disease, although not without challenges, is a possibility.
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Doença de Alzheimer/prevenção & controle , Vacinas contra Alzheimer/classificação , Peptídeos beta-Amiloides/metabolismo , Placa Amiloide/imunologia , Doença de Alzheimer/patologia , Anticorpos Monoclonais/uso terapêutico , Encéfalo/patologia , Ensaios Clínicos como Assunto/tendências , Humanos , Emaranhados Neurofibrilares , Vacinação/métodosRESUMO
Os indicadores e medidas de saúde demonstram o nível de saúde da população, medem a frequência ou a probabilidade da ocorrência de novos casos de doença na população. Assim, este trabalho buscou analisar medidas de saúde do município de Cupira, Pernambuco, visando elaborar um prévio diagnóstico da situação saúde da população referente às doenças de notificação compulsória. As informações foram obtidas no Sistema de Informação de Agravos de Notificação (SINAN) do município de Cupira, nos anos de 2007 a 2011, no período de janeiro a dezembro. As enfermidades registradas como confirmadas no período de estudo foram: esquistossomose (38,9/10.000 hab.), dengue (33,3 casos/10.000 hab.), hanseníase (12,3 casos/10.000 hab.), tuberculose (9,4 casos/10.000 hab.), doença de chagas guda (2,56/10.000 hab.), leishmaniose tegumentar americana (1,28 casos/10.000 hab.), malária (1,6/10.000 hab.), meningite (0,85/10.000 hab.) e leishmaniose visceral (0,5 casos/10.000 hab.). Em relação &a
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Os indicadores e medidas de saúde demonstram o nível de saúde da população, medem a frequência ou a probabilidade da ocorrência de novos casos de doença na população. Assim, este trabalho buscou analisar medidas de saúde do município de Cupira, Pernambuco, visando elaborar um prévio diagnóstico da situação saúde da população referente às doenças de notificação compulsória. As informações foram obtidas no Sistema de Informação de Agravos de Notificação (SINAN) do município de Cupira, nos anos de 2007 a 2011, no período de janeiro a dezembro. As enfermidades registradas como confirmadas no período de estudo foram: esquistossomose (38,9/10.000 hab.), dengue (33,3 casos/10.000 hab.), hanseníase (12,3 casos/10.000 hab.), tuberculose (9,4 casos/10.000 hab.), doença de chagas guda (2,56/10.000 hab.), leishmaniose tegumentar americana (1,28 casos/10.000 hab.), malária (1,6/10.000 hab.), meningite (0,85/10.000 hab.) e leishmaniose visceral (0,5 casos/10.000 hab.). Em relação &a
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The prevalence of T. gondii infection was investigated in free-range chickens for human consumption in the county of Rio Bonito, Rio de Janeiro, Brazil. Thus, a search for antibodies to T. gondii by indirect fluorescent antibody test was done in the serum of 220 chickens from twenty two farms. Titres equal or greater than 16 were considered positive. The prevalence of toxoplasmic infection found in chickens was 27.6% (61/220). There were positive cases in eighteen (77.2%) farms. The more frequent titre was 16 (12.5%) and the highest was 4096 (0.4%). The high prevalence of T. gondii in chickens and the high percentage of farms with positive cases indicate environmental contamination in the studied area showing the possibility of human and animal infection.
Assuntos
Animais , Toxoplasma/isolamento & purificação , Galinhas/parasitologia , Toxoplasmose Animal/epidemiologia , Brasil , Técnica Indireta de Fluorescência para Anticorpo/veterináriaRESUMO
The prevalence of T. gondii infection was investigated in free-range chickens for human consumption in the county of Rio Bonito, Rio de Janeiro, Brazil. Thus, a search for antibodies to T. gondii by indirect fluorescent antibody test was done in the serum of 220 chickens from twenty two farms. Titres equal or greater than 16 were considered positive. The prevalence of toxoplasmic infection found in chickens was 27.6% (61/220). There were positive cases in eighteen (77.2%) farms. The more frequent titre was 16 (12.5%) and the highest was 4096 (0.4%). The high prevalence of T. gondii in chickens and the high percentage of farms with positive cases indicate environmental contamination in the studied area showing the possibility of human and animal infection.
RESUMO
Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 100,000 subunits of 50 kDa flagellin that can have diversebiotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy.The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up.