RESUMO
BACKGROUND: The aim of this work was to determine morphometrical changes in lingual, labial and submaxillar salivary glands from alcoholic chronics. METHODS: Five samples of each type of gland were obtained from autopsies of chronic alcoholics and equal number of samples from individuals whose death cause was accidental (controls). Serous acini in von Ebner and submaxillar glands and mucous acini in labial and Blandin-Nühn glands were analysed. In the ductal system, intra and interlobular striated ducts were studied. A digital-image analyser was used to measure different parameters in the acini and ducts. RESULTS: Statistical analysis revealed that lingual and submaxillar salivary glands presented a significant acinar hypertrophy and hyperplasy. These changes were not observed in labial salivary glands. All studied glands showed significant structural modifications in the striated ducts. CONCLUSION: These results indicate that the significant variations together with the histological qualitative pattern could be useful as confident indicators for the differential histopathological diagnosis with other sialosis from different aetiology.
Assuntos
Alcoolismo/patologia , Doenças das Glândulas Salivares/patologia , Glândulas Salivares Menores/patologia , Doenças da Glândula Submandibular/patologia , Adulto , Idoso , Estudos de Casos e Controles , Tamanho Celular , Epitélio/patologia , Feminino , Humanos , Hiperplasia , Hipertrofia , Processamento de Imagem Assistida por Computador , Masculino , Pessoa de Meia-Idade , Ductos Salivares/patologiaRESUMO
El presente trabajo se realizó con el objeto de corroborar y relacionar los aspectos estructurales de los molares primarios observados con MO y MEB y aplicar las técnicas de histoquímica microanalítica para determinar los patrones cuantitativos de mineralización del esmalte y dentina. Se utilizó un total de 30 molares (1º y 2º) clínicamente sanos, exfoliados. El estudio para microscopía óptica (MO) fue mediante la técnica por desgaste y cortes procesados y metalizados para la electrónica de barrido (MEB). En el esmalte se identificó la zona aprismática periférica, la línea neonatal, abundantes husos, conductillos dentinarios remanentes y microdefectos (laminillas o cracks de morfología y longitud variables). La dentina se caracterizó por la amplitud de los túbulos y escasa o nula dentina intertubular a nivel circumpulpar. El microanálisis con energía dispersiva de rayos X puso de relieve que el índice calcio/fósforo, tanto en esmalte como en dentina, alcanza el valor de 1.7, lo que sugiere que en la configuración de los cristales de apatita existen aniones carbonato. Los hallazgos de frecuentes microdefectos y la presencia de numerosas estructuras sin esmalte (husos y conductillos penetrantes) en la conexión amelodentinaria, especialmente en la porción coronaria oclusal nos advierte de los cuidados o precauciones a tomar en el momento de usar ácidos grabadores. Asimismo, la existencia de patrones de mineralización similares entre elementos primarios y permanentes, permite inferir que la terapéutica odontológica basada en la naturaleza de lamineralización podría aplicarse con idénticas pautas, pero respetando las variaciones estructurales respectivas (AU)
Assuntos
Pré-Escolar , Criança , Dente Decíduo/ultraestrutura , Dente Molar/ultraestrutura , Dentina/ultraestrutura , Esmalte Dentário/ultraestrutura , Microscopia de Polarização , Microscopia Eletrônica de Varredura , Microanálise por Sonda Eletrônica , Histocitoquímica/métodos , Estudos de Avaliação como Assunto , Interpretação Estatística de DadosRESUMO
El presente trabajo se realizó con el objeto de corroborar y relacionar los aspectos estructurales de los molares primarios observados con MO y MEB y aplicar las técnicas de histoquímica microanalítica para determinar los patrones cuantitativos de mineralización del esmalte y dentina. Se utilizó un total de 30 molares (1º y 2º) clínicamente sanos, exfoliados. El estudio para microscopía óptica (MO) fue mediante la técnica por desgaste y cortes procesados y metalizados para la electrónica de barrido (MEB). En el esmalte se identificó la zona aprismática periférica, la líne
Assuntos
Pré-Escolar , Criança , Dente Decíduo/ultraestrutura , Dente Molar/ultraestrutura , Dentina/ultraestrutura , Esmalte Dentário/ultraestrutura , Microscopia de Polarização , Microscopia Eletrônica de Varredura , Microanálise por Sonda Eletrônica , Histocitoquímica/métodos , Estudos de Avaliação como Assunto , Interpretação Estatística de DadosRESUMO
El presente trabajo se realizó con el objeto de corroborar y relacionar los aspectos estructurales de los molares primarios observados con MO y MEB y aplicar las técnicas de histoquímica microanalítica para determinar los patrones cuantitativos de mineralización del esmalte y dentina. Se utilizó un total de 30 molares (1º y 2º) clínicamente sanos, exfoliados. El estudio para microscopía óptica (MO) fue mediante la técnica por desgaste y cortes procesados y metalizados para la electrónica de barrido (MEB). En el esmalte se identificó la zona aprismática periférica, la línea neonatal, abundantes husos, conductillos dentinarios remanentes y microdefectos (laminillas o cracks de morfología y longitud variables). La dentina se caracterizó por la amplitud de los túbulos y escasa o nula dentina intertubular a nivel circumpulpar. El microanálisis con energía dispersiva de rayos X puso de relieve que el índice calcio/fósforo, tanto en esmalte como en dentina, alcanza el valor de 1.7, lo que sugiere que en la configuración de los cristales de apatita existen aniones carbonato. Los hallazgos de frecuentes microdefectos y la presencia de numerosas estructuras sin esmalte (husos y conductillos penetrantes) en la conexión amelodentinaria, especialmente en la porción coronaria oclusal nos advierte de los cuidados o precauciones a tomar en el momento de usar ácidos grabadores. Asimismo, la existencia de patrones de mineralización similares entre elementos primarios y permanentes, permite inferir que la terapéutica odontológica basada en la naturaleza de lamineralización podría aplicarse con idénticas pautas, pero respetando las variaciones estructurales respectivas
Assuntos
Pré-Escolar , Criança , Esmalte Dentário , Dentina , Dente Molar , Dente Decíduo , Microanálise por Sonda Eletrônica , Histocitoquímica/métodos , Microscopia Eletrônica de Varredura , Microscopia de Polarização , Estudos de Avaliação como Assunto , Interpretação Estatística de DadosRESUMO
BACKGROUND: Chronic alcoholism has been associated with structural and physiological changes in salivary glands. Studies on a variety of pathologies have suggested that variation in number of nucleolar organizer regions (NORs) reveals conditions of cellular activity. The aim of this work was to examine, through the AgNOR technique, changes in number and size of NORs in lingual salivary glands of chronic alcoholics. METHODS: Samples of mucous and serous lingual salivary glands were obtained from tongues from autopsies of individuals whose cause of death was hepatic alcoholic cirrhosis. Lingual organs from individuals whose cause of death was accidental were used as controls. Number and size of the AgNORs and nuclear area, in ductal and acinar cells, were evaluated through a digital image analyzer. RESULTS: Statistical analysis revealed differences (P < or = 0.05) in number of AgNORs in mucous acini and ductal cells. Also, we observed changes in the area of the NORs. CONCLUSION: These results suggest that in alcoholics the activity of glandular cells, mainly in ductal epithelium, could be affected, modifying synthesis, transport and salivary secretions.
Assuntos
Alcoolismo/patologia , Região Organizadora do Nucléolo/patologia , Glândulas Salivares Menores/patologia , Idoso , Alcoolismo/complicações , Estudos de Casos e Controles , Humanos , Masculino , Pessoa de Meia-Idade , Região Organizadora do Nucléolo/genética , Saliva/metabolismo , Doenças das Glândulas Salivares/etiologia , Coloração pela Prata , Estatísticas não Paramétricas , Língua , Transcrição GênicaRESUMO
Se analizaron los cambios estructurales de los distintos componentes del CATM en fetos humanos de 16, 18, 24 y 37 semanas de gestación, con el propósito de identificar el grado de maduración tisular y asociarlo con su posible capacidad funcional para efectuar los movimientos mandibulares. Se emplearon técnicas histoquímicas de rutina y de inmunomarcación. Los anticuerpos utilizados fueron proteína de neurofilamentos (NE14), vimentina y mioglobina mediante el sistema avidina-biotina, DAB. Las variaciones observadas en las distintas edades gestacionales se encontraron principalmente, a nivel del disco articular y cóndilo mandibular. Entre las 16 y 18 semanas, el disco mostró fibrillas PAS positivas y abundantes fibras argirófilas dispuestas en plexos. Las fibras elásticas fueron escasas y muy delgadas en la zona bilaminar póstero-superior. A las 24 semanas, el CATM mostró un aumento notable en las dimensiones de todos sus componentes. El disco de mayor espesor exibió una proporción más elevada de fibras colágenas y elásticas, mientras que las fibrillas argirófilas organizadas en haces adquirieron una disposición específica. Se identificaron nervios inmunorreactivos con NE14 en la zona anterior y en el espacio laminar póstero-inferior próximo al cóndilo. En los especímenes de mayor edad, los cortes sagitales del disco aislado, mostraron diferentes regiones según el espesor y la disposición de las fibras colágenas. Algunos haces musculares del pterigoideo dispuestos en zig-zag y con apariencia de un estado de semicontracción, no expresaron la mioglobina. A las 24 semanas, los componentes del CATM presentaron, en general, características similares a las observadas en los fetos de término. La maduración de los músculos asociados y de los tejidos articulares, en especial del disco, permite sugerir que los componentes del CATM estarían capacitados para desarrollar actividades de apertura y cierre mandibular a partir de esta etapa prenatal.
Assuntos
Humanos , Articulação Temporomandibular/crescimento & desenvolvimento , Articulação Temporomandibular/fisiologia , Desenvolvimento Embrionário e Fetal , Articulação Temporomandibular/embriologia , DissecaçãoAssuntos
Humanos , Adulto , Pessoa de Meia-Idade , Idoso , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Neoplasias Encefálicas/secundário , Neoplasias Pulmonares/patologia , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Taxa de Sobrevida , Argentina , Cérebro , Resultado do TratamentoRESUMO
In the present study we investigated tissue changes in palatine (PG) and labial (LG) minor salivary glands from individuals who had died of alcoholic hepatic cirrhosis, to characterize histopathological parameters of alcoholic sialosis, that may be used for differential diagnosis. Samples obtained from autopsies were processed using cytochemical techniques for mucosubstances and immunocytochemical labelling for cytokeratines, PS 100 and T-cells. Both PG and LG showed dilated excretory ducts with atrophic epithelium, which contained PAS+ metachromatic material and detached cells. Intra and interlobular ductal hyperplasia was present in some areas, mainly in PG. CK expression was heterogeneous in ductal cells, and negative in acinar cells. Most of the acinar cell nuclei were normal, but some of them were atypical in shape and distribution. Myoepithelial cells, serous demilunes and the basal region of the cells of the striated ducts expressed PS 100. In PG, 85% of the mononuclear infiltrates expressed T-cell markers, whereas in LG only 40% of these cells were T-cells. These findings, in addition to other histopathological parameters seen in previous studies, may be used as indicators for differential diagnosis with other gland pathologies.
Assuntos
Alcoolismo/patologia , Ductos Salivares/patologia , Doenças das Glândulas Salivares/diagnóstico , Glândulas Salivares Menores/patologia , Idoso , Alcoolismo/complicações , Estudos de Casos e Controles , Diagnóstico Diferencial , Humanos , Técnicas Imunoenzimáticas , Queratinas/análise , Cirrose Hepática Alcoólica , Masculino , Pessoa de Meia-Idade , Proteínas S100/análise , Doenças das Glândulas Salivares/etiologia , Linfócitos TRESUMO
In the present study we investigated tissue changes in palatine (PG) and labial (LG) minor salivary glands from individuals who had died of alcoholic hepatic cirrhosis, to characterize histopathological parameters of alcoholic sialosis, that may be used for differential diagnosis. Samples obtained from autopsies were processed using cytochemical techniques for mucosubstances and immunocytochemical labelling for cytokeratines, PS 100 and T-cells. Both PG and LG showed dilated excretory ducts with atrophic epithelium, which contained PAS+ metachromatic material and detached cells. Intra and interlobular ductal hyperplasia was present in some areas, mainly in PG. CK expression was heterogeneous in ductal cells, and negative in acinar cells. Most of the acinar cell nuclei were normal, but some of them were atypical in shape and distribution. Myoepithelial cells, serous demilunes and the basal region of the cells of the striated ducts expressed PS 100. In PG, 85
of the mononuclear infiltrates expressed T-cell markers, whereas in LG only 40
of these cells were T-cells. These findings, in addition to other histopathological parameters seen in previous studies, may be used as indicators for differential diagnosis with other gland pathologies.
RESUMO
In the present study we investigated tissue changes in palatine (PG) and labial (LG) minor salivary glands from individuals who had died of alcoholic hepatic cirrhosis, to characterize histopathological parameters of alcoholic sialosis, that may be used for differential diagnosis. Samples obtained from autopsies were processed using cytochemical techniques for mucosubstances and immunocytochemical labelling for cytokeratines, PS 100 and T-cells. Both PG and LG showed dilated excretory ducts with atrophic epithelium, which contained PAS+ metachromatic material and detached cells. Intra and interlobular ductal hyperplasia was present in some areas, mainly in PG. CK expression was heterogeneous in ductal cells, and negative in acinar cells. Most of the acinar cell nuclei were normal, but some of them were atypical in shape and distribution. Myoepithelial cells, serous demilunes and the basal region of the cells of the striated ducts expressed PS 100. In PG, 85
of the mononuclear infiltrates expressed T-cell markers, whereas in LG only 40
of these cells were T-cells. These findings, in addition to other histopathological parameters seen in previous studies, may be used as indicators for differential diagnosis with other gland pathologies.
RESUMO
Samples from parotid, submaxillary, and von Ebner salivary glands of six chronic alcoholic individuals who had died of alcoholic hepatic cirrhosis were analyzed by topographic and histochemical routine stains and marked for cytokeratins; two normal adult individuals were used as control. Modifications in the acinar cells were found, but the most evident changes were observed in the ductal system: enlargement of major ducts, heterogeneous expression of cytokeratins and athrophy in epithelial cells, desquamated cells and stasis of content, and ductal hyperplasia in von Ebner glands. The lymphoplasmocytic infiltration does not represent the typical lymphocytic focus on Sjögren's syndrome or other connective tissue pathologies. Our findings indicate that functional and structural variations are produced both in serous acini and ducts parotid, submaxilar and von Ebner glands affected by alcoholic sialosis.
Assuntos
Alcoolismo/patologia , Glândula Parótida/patologia , Glândulas Salivares Menores/patologia , Glândula Submandibular/patologia , Adulto , Idoso , Cadáver , Células Epiteliais/patologia , Humanos , Queratinas/análise , Linfócitos/patologia , Pessoa de Meia-Idade , Ductos Salivares/patologiaRESUMO
Samples from parotid, submaxillary, and von Ebner salivary glands of six chronic alcoholic individuals who had died of alcoholic hepatic cirrhosis were analyzed by topographic and histochemical routine stains and marked for cytokeratins; two normal adult individuals were used as control. Modifications in the acinar cells were found, but the most evident changes were observed in the ductal system: enlargement of major ducts, heterogeneous expression of cytokeratins and athrophy in epithelial cells, desquamated cells and stasis of content, and ductal hyperplasia in von Ebner glands. The lymphoplasmocytic infiltration does not represent the typical lymphocytic focus on Sj÷grens syndrome or other connective tissue pathologies. Our findings indicate that functional and structural variations are produced both in serous acini and ducts parotid, submaxilar and von Ebner glands affected by alcoholic sialosis.
RESUMO
Samples from parotid, submaxillary, and von Ebner salivary glands of six chronic alcoholic individuals who had died of alcoholic hepatic cirrhosis were analyzed by topographic and histochemical routine stains and marked for cytokeratins; two normal adult individuals were used as control. Modifications in the acinar cells were found, but the most evident changes were observed in the ductal system: enlargement of major ducts, heterogeneous expression of cytokeratins and athrophy in epithelial cells, desquamated cells and stasis of content, and ductal hyperplasia in von Ebner glands. The lymphoplasmocytic infiltration does not represent the typical lymphocytic focus on Sj÷grens syndrome or other connective tissue pathologies. Our findings indicate that functional and structural variations are produced both in serous acini and ducts parotid, submaxilar and von Ebner glands affected by alcoholic sialosis.
RESUMO
We here in determined the histochemical and immunological changes that salivary glands experience as a result of the ageing process. Samples from the inferior lip of children, youngsters and young adults were analyzed by histochemical techniques, Periodic-acid-Schiff, (PAS) Alcian blue (AB) (pHs 1.0 and 2.5) and Toluidine blue for mucosubstances and immunohistochemical staining of S 100 protein and cytokeratin 20, avidin-biotin system (DAB). In children, the techniques used evidenced various reaction degrees in the acinar cells, even within a single acinous. They displayed a slight metachromasia and were alcianophilic at pH 2.5. In youngsters, and especially in adults, glands showed a notable PAS positivity and alcianophilia at both pH levels, and an intense metachromasia. PS 100 was positive in the basal area of the acini and serous demilunes of all groups, the reactivity being higher in adults. Cytokeratin 20 was better observed in ductal cells from children glands. These findings suggest modifications at cytological level and in the chemical composition of the secretory granules, indicating possible functional variations in lip salivary glands related to the ageing process.
Assuntos
Envelhecimento/fisiologia , Glândulas Salivares Menores/química , Glândulas Salivares Menores/citologia , Adolescente , Adulto , Anticorpos Monoclonais , Criança , Histocitoquímica , Humanos , Queratinas/análise , Lábio , Proteínas S100/análise , Vesículas SecretóriasRESUMO
The histogenesis of the salivary glands was structurally and cytochemically studied in human embryos and fetuses during the period of 8 to 32 weeks of intrauterine life. Glandular buds appeared at about 12 weeks of embryonic development. The rounded distal ends of the epithelial cords and neighbouring mesenchyma showed small and abundant PAS positive and alcianophilic granules. At age 14 weeks the secretory end pieces and the duct system were seen at different morphologic and structural stages of a differentiation. Mucous acini with scanty mixed acini predominated and serous acini appeared occasionally. From 20 to 24 weeks the mucous acini stained with toluidine blue featured different degrees of metachromasia even in the case of cells of the same acinus. In the ducts it was also possible to identify metachromatic cells intermingled with basophilic cells in the epithelial coat. These findings suggest that the palatine glands present typical histophysiological material from 14 to 20 weeks. The presence of PAS positive, alcianophilic and metachromatic secretory substance in the acinar lumen and the luminal content of ducts suggests that mucin secretion begins during intrauterine life.
Assuntos
Glândulas Exócrinas/embriologia , Glândulas Salivares Menores/embriologia , Desenvolvimento Embrionário e Fetal , Humanos , Mucinas/metabolismo , PalatoRESUMO
La histogénesis de las glándulas palatinas fue estudiada desde el punto de vista estructural y citoquímico en embriones y fetos humanos de 8 a 32 semanas de desarrollo. Los primordios glandulares aparecían a las 12 semanas de vida intrauterina. Los extremos distales redondeados de los cordones epiteliales y el mesénquima circundante mostraban abundantes gránulos PAS positivos y alcianofílicos. A las 14 semanas las piezas secretorias terminales y el sistema ductal presentaban diferentes estadios de diferenciación estructural. Predominaban los acinos mucosos con escasos acinos mixtos, en tanto que los acinos serosos aparecían ocasionalmente. Entre las 20 y 24 semanas los acinos mucosos coloreados con azul de toluidina presentaban diferentes grados de metacromasia aún dentro de células de un mismo acino. En los conductos se identificaban células metacromáticas intercaladas con células basófilas en la cubierta epitelial. Estos hallazgos sugieren que las glándulas palatinas presentan un material histofisiológico típico entre las 14 y 20 semanas. La presencia de sustancias secretorias PAS positivas, alcianofílicas y metacromáticas en la luz acinar y contenido luminal de los conductos nos sugiere que la secreción de mucinas se inicia en la vida intrauterina
Assuntos
Humanos , Desenvolvimento Fetal/fisiologia , Feto/embriologia , Glândulas Salivares/citologia , Glândulas Salivares/embriologia , Palato , Histocitoquímica/métodosRESUMO
The histogenesis of the salivary glands was structurally and cytochemically studied in human embryos and fetuses during the period of 8 to 32 weeks of intrauterine life. Glandular buds appeared at about 12 weeks of embryonic development. The rounded distal ends of the epithelial cords and neighbouring mesenchyma showed small and abundant PAS positive and alcianophilic granules. At age 14 weeks the secretory end pieces and the duct system were seen at different morphologic and structural stages of a differentiation. Mucous acini with scanty mixed acini predominated and serous acini appeared occasionally. From 20 to 24 weeks the mucous acini stained with toluidine blue featured different degrees of metachromasia even in the case of cells of the same acinus. In the ducts it was also possible to identify metachromatic cells intermingled with basophilic cells in the epithelial coat. These findings suggest that the palatine glands present typical histophysiological material from 14 to 20 weeks. The presence of PAS positive, alcianophilic and metachromatic secretory substance in the acinar lumen and the luminal content of ducts suggests that mucin secretion begins during intrauterine life.