RESUMO
La realización de un nuevo tratamiento luego de un fracaso con implantes dentarios representa un gran desafío para el profesional especialistas en implan-tología, así como para el paciente que debe someter-se a un nuevo procedimiento para rehabilitarse. En estos casos, el diagnóstico y la planificación exhaus-tiva previa nos permiten optimizar el tiempo y los re-cursos, arribando al resultado esperado de forma eficiente. En este reporte de caso se presenta la re-habilitación de una paciente que acude a la consulta con un fracaso implantario previo y con alta deman-da estética. Se diseñó una guía quirúrgica de preci-sión para la colocación de un implante Straumann®Bone Level Tapered 3,3 NC Narrow CrossFit® ø 3,3 mm 8 mm junto a la regeneración ósea guiada para compensar la deficiencia de los tejidos circundantes. La rehabilitación protésica consistió en una corona cemento atornillada en disilicato de litio. En el control al año pudo valorarse la estabilidad de la rehabilita-ción protésica, así como de los tejidos circundantes y el contorno estético junto al perfil de emergencia mimético logrado al final del tratamiento (AU)
Carrying out a new treatment after a failure with dental implants represents a great challenge for the implantology specialist professional, as well as for the patient who must undergo a new procedure to rehabilitate. In these cases, the diagnosis and prior exhaustive planning allow us to optimize time and resources, arriving at the expected result efficiently. This case report presents the rehabilitation of a patient who comes to the clinic with a previous implant failure and with high aesthetic demand. A precision surgical guide was designed for the placement of a Straumann® Bone Level Tapered 3.3 NC Narrow CrossFit® ø 3.3 mm 8 mm implant together with guided bone regeneration to compensate for the deficiency of the surrounding tissues. The prosthetic rehabilitation consisted of a screw-retained cement crown in lithium disilicate. In the one-year control, the stability of the prosthetic rehabilitation, as well as the surrounding tissues and the aesthetic contour, together with the mimetic emergence profile achieved at the end of the treatment, could be assessed (AU)
Assuntos
Humanos , Feminino , Adulto , Planejamento de Assistência ao Paciente , Regeneração Óssea , Regeneração Tecidual Guiada Periodontal/métodos , Falha de Restauração Dentária , Cirurgia Assistida por Computador/métodos , Implantação Dentária Endóssea/efeitos adversos , Seguimentos , Silicatos , Coroas , Projeto do Implante Dentário-Pivô , Membranas ArtificiaisRESUMO
AIMS: To assess the inhibitory activity on Gram-positive and Gram-negative bacteria of several species of enterococci recovered from a natural corn silage. METHODS AND RESULTS: The inhibitory activity of strains of Enterococcus faecalis (58), Enterococcus faecium (35), Enterococcus gallinarum (3) and Enterococcus casseliflavus (4) were studied employing indicator strains from various sources (clinical, food and ATCC). Enterococcus faecalis MR99, the only strain with inhibitory activity, inhibited other enterococci, Listeria spp., Staphylococcus aureus, Clostridium spp., Bacillus spp., Escherichia coli, Shigella sonnei and Shigella flexneri. The bacterium contained only one conjugative pheromone-responsive plasmid. The partially chromatography-purified MR99 enterocin (PPE) had a molecular weight of approx. 5000 Da and a pI of 6.2, was sensitive to proteolytic enzymes and could be extracted in benzene and butanol. It appeared stable to adjustment of pH 4.0, 5.0, 6.0, 7.0 and 8.0 and was resistant to heat. Inactivation was at 15 min at 121 degrees C. Enterocin MR99 was bactericidal on strains of Listeria monocytogenes, Staph. aureus, and bovine mastitis agents, it was bacteriostatic on E. coli. Although enterocins MR99 and AS48 have inhibitory activity on Gram-negative bacilli, PCR studies demonstrated a lack of relationship between them. CONCLUSIONS: The active component had a protein nature, was resistant to heat and presented a wide inhibitory spectrum. SIGNIFICANCE AND IMPACT OF THE STUDY: The biological properties of Ent. faecalis MR99 suggest that this strain merits further investigations so it can be applied in human and veterinary health programmes.
Assuntos
Bacteriocinas/farmacologia , Enterococcus/fisiologia , Microbiologia de Alimentos , Silagem/microbiologia , Zea mays/microbiologia , Bacillus/efeitos dos fármacos , Hidrocarbonetos Aromáticos com Pontes/análise , Hidrocarbonetos Aromáticos com Pontes/farmacologia , Clostridium/efeitos dos fármacos , Meios de Cultura , Eletroforese em Gel de Poliacrilamida/métodos , Enterococcus faecalis/fisiologia , Enterococcus faecium/fisiologia , Escherichia coli/efeitos dos fármacos , Focalização Isoelétrica/métodos , Listeria/efeitos dos fármacos , Listeria monocytogenes/efeitos dos fármacos , Listeria monocytogenes/ultraestrutura , Microscopia Eletrônica/métodos , Plasmídeos/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Shigella/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/ultraestruturaRESUMO
The aims of this study were to characterize human American tegumentary leishmaniasis, which includes cutaneous, mucocutaneous and mucosal leishmaniasis, in Northwest Argentina, to determine the prevalence of double infection with Trypanosoma cruzi and to identify the species of Leishmania in this area. Most of the 330 leishmaniasis patients presented cutaneous ulcers (96.1%), 2.4% mucocutaneous and 1.5% the mucosal form ('espundia'). The aetiological agents, determined by isoenzyme electrophoresis, were identified as Leishmania (Viannia) braziliensis in 16 out of 20 isolates and in the remaining 4 as Leishmania (Leishimania) amazonensis, the first ever-documented in Argentina. Sera analysed by ELISA and IFA using complex antigen from both T. cruzi and L. braziliensis showed a very high percentage of positives (66.3-78.2%). When antigens for specific diagnosis of Chagas' disease were used, 40.9% of the leishmaniasis patients were also found to be infected by T. cruzi. These results indicate that the strong immune response against T. cruzi gave no protection to Leishmania, in spite of the serological cross-reaction between these parasites. In addition, we showed that more than 40% of the patients would be misdiagnosed as chagasic if complex antigens, as epimastigotes or soluble fraction from epimastigotes, were used in IFA or ELISA. This is of paramount importance not only because patients' treatment would be associated to misdiagnosis but the fact that in many countries in Central and South America, a positive test for Chagas' disease means a rejection for those seeking employment.
Assuntos
Doença de Chagas/complicações , Doença de Chagas/imunologia , Leishmania/classificação , Leishmania/isolamento & purificação , Leishmaniose Cutânea/complicações , Leishmaniose Cutânea/imunologia , Trypanosoma cruzi/isolamento & purificação , Animais , Argentina/epidemiologia , Doença de Chagas/diagnóstico , Doença de Chagas/parasitologia , Reações Cruzadas , Feminino , Genes de Protozoários/genética , Humanos , Leishmania/enzimologia , Leishmania/genética , Leishmaniose Cutânea/diagnóstico , Leishmaniose Cutânea/parasitologia , Masculino , FilogeniaRESUMO
In a study, carried out in 2000, of the clinical and parasitological status of a Wichi Aboriginal community living in the suburbs of Tartagal, northern Salta, Argentina, 154 individuals were screened for parasitic infections. Ninety-five faecal samples were also obtained from the same population. Ninety-three percent of the subjects were positive for 1 or more of the parasites investigated by direct test and 70.5% of them had parasitic superinfection. The most frequent helminths were Strongyloides stercoralis (50.5%) and hookworm (47.4%). We found low reinfection rates and a long reinfection period after treatment and provision of safe water and sanitation. Serum reactivity of these patients was analysed by enzyme-linked immunosorbent assay and indirect immunofluorescent assay and 22.1% of them had anti-Toxocara antibodies, 16.2% were positive for a complex antigen of Leishmania braziliensis, 29.9% were positive for a complex Trypanosoma cruzi antigen, and 17.5% were positive for a specific Trypanosoma cruzi antigen, Ag 163B6/cruzipain.
Assuntos
Indígenas Sul-Americanos/etnologia , Doenças Parasitárias/etnologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anemia/etnologia , Anemia/parasitologia , Argentina/epidemiologia , Criança , Pré-Escolar , Eosinofilia/etnologia , Eosinofilia/parasitologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Doenças Parasitárias/diagnósticoRESUMO
Some Leishmania species affect humans in two principal forms: visceral and cutaneous leishmaniosis (CL). Several studies have identified dogs as the main reservoirs of the visceral leishmaniosis (VL) caused by Leishmania infantum. The purpose of this work was to carry out a survey of the canine population associated with human cases of American tegumentary leishmaniosis (ATL), in order to establish the clinical, parasitological, serological and immunological characteristics of the canine disease, in an endemic region for both ATL and Chagas' disease in the province of Salta, in northwestern Argentina. Two hundred and eight dogs from the endemic area were examined and 41 (19.7%) of them presented lesions compatible with leishmaniosis. In order to investigate the presence of antibodies against Leishmania spp. and Trypanosoma cruzi, sera were screened by ELISA using two complex antigens from these parasites and, because of cross-reactions between them, a specific antigen for diagnosis of T. cruzi infection. Sixty-two (29.8%) of 208 dogs were positive for the complex antigen F45 from Leishmania and 50 (24%) were positive for the complex antigen F105 from T. cruzi. Nine dogs (4.3%) were positive for the specific Ag163B6-cruzipain suggesting that these dogs were truly infected with T. cruzi. Furthermore, three of these nine dogs presented Leishmania sp. in their skin lesions and therefore were considered as infected by both, T. cruzi and Leishmania parasites. The prevalence of Leishmania infection detected by lesions and/or positive serology was 27.4% (57/208). On the basis of previous observations regarding the clustered appearance of human ATL, the dog population was divided into two groups: zone A, dogs living within a 100 m radius from houses with human cases, and zone B, dogs living beyond this limit. The prevalence of ATL in dogs was significantly higher in zone A (34.6%) than in zone B (7.3%), suggesting a strong correlation between canine and human cases. The average time required for a parasitological diagnosis by microscopy was six times longer for dog samples than human ones, and the average number of parasites per 100 microscopic fields was 14-fold lower in canine samples. The high prevalence of Leishmania infection and the close association with human cases, demonstrated that dogs are a very susceptible host for Leishmania infection, but the scarcity of parasites in their lesions suggests that they may not be the main reservoir of the parasite in this endemic area.
Assuntos
Reservatórios de Doenças/veterinária , Doenças do Cão/parasitologia , Doenças Endêmicas , Leishmania infantum/isolamento & purificação , Leishmaniose Cutânea/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Argentina/epidemiologia , Biópsia/veterinária , Doenças do Cão/epidemiologia , Doenças do Cão/transmissão , Cães , Ensaio de Imunoadsorção Enzimática , Humanos , Hipersensibilidade Tardia/parasitologia , Hipersensibilidade Tardia/veterinária , Leishmaniose Cutânea/sangue , Leishmaniose Cutânea/epidemiologia , Leishmaniose Cutânea/parasitologia , Leishmaniose Cutânea/transmissão , Estudos Soroepidemiológicos , Pele/parasitologia , Trypanosoma cruzi/parasitologiaRESUMO
A clinical-serological follow-up was carried out in a canine population in endemic foci of Leishmania braziliensis spread in northwestern Argentina. Each dog was studied in at least two visits, 309+/-15 days (X+/-SE) apart. Some initially healthy dogs (n=52) developed seroconversion or lesions. The clinical evolution of the disease in dogs resembles in many aspects the human disease. Similarities include the long duration of most ulcers with occasional healing or appearance of new ones and the late appearance of erosive snout lesions in some animals. Yearly incidence rates of 22.7% for seroconversion and of 13.5% for disease were calculated as indicators of the force of infection by this parasite upon the canine population.
Assuntos
Doenças do Cão/parasitologia , Leishmania braziliensis/patogenicidade , Leishmaniose Cutânea/veterinária , Animais , Argentina , Reservatórios de Doenças/veterinária , Doenças do Cão/patologia , Cães , Seguimentos , Leishmaniose Cutânea/patologia , Estudos LongitudinaisRESUMO
Cruzipain, the major proteinase of Trypanosoma cruzi, plays an important role in the biology of this parasite. This study reports the development of a recombinant Fab antibody, using RNA isolated from the anti-Ag163B6 hybridoma against cruzipain. This procedure involves the use of cDNAs obtained with the aid of a specific set of primers complementary to the complete light kappa chain (L kappa) and the first two domains of the IgG1 heavy chain (VH/CH1). These products were subsequently cloned in the pComb3 system, from which the gIII gene had been removed, and expressed in Escherichia coli cells. The recombinant Fab molecule recognized cruzipain by ELISA, in a fashion similar to the original mAb anti-Ag163B6. Nucleotide sequence analysis of the recombinant molecule, together with its immunological recognition by specific anti-mouse IgG (Fab)2, indicated the immunoglobulin nature of the recombinant product. Moreover, both the mAb anti-Ag163B6 and the soluble Fab fragment described here react similarly with the intact parasite surface, as observed in an indirect immunofluorescence assay. In conclusion, our recombinant Fab anti-Ag 163B6 allows the possible use of this molecule for diagnosis, antigen purification, and eventually treatment of Chagas-afflicted individuals.
Assuntos
Antígenos de Protozoários/imunologia , Cisteína Endopeptidases/imunologia , Fragmentos Fab das Imunoglobulinas/biossíntese , Fragmentos Fab das Imunoglobulinas/genética , Proteínas Recombinantes/biossíntese , Trypanosoma cruzi/imunologia , Animais , Reações Antígeno-Anticorpo , Western Blotting , Clonagem Molecular , Ensaio de Imunoadsorção Enzimática , Técnica Indireta de Fluorescência para Anticorpo , Vetores Genéticos/síntese química , Fragmentos Fab das Imunoglobulinas/isolamento & purificação , Imunoglobulina G/biossíntese , Imunoglobulina G/genética , Cadeias Pesadas de Imunoglobulinas/biossíntese , Cadeias Pesadas de Imunoglobulinas/genética , Cadeias Leves de Imunoglobulina/biossíntese , Cadeias Leves de Imunoglobulina/genética , Dados de Sequência Molecular , Proteínas de Protozoários , Proteínas Recombinantes/isolamento & purificaçãoRESUMO
An epidemic neuropathy in Cuba has caused bilateral optic neuropathies in more than 26,000 people during the past three years. Various pathogenetic factors have been proposed, including toxins, nutritional deficiencies, and an underlying genetic predisposition involving mitochondrial DNA. As part of a case-control collaborative investigation, 135 Cuban blood samples were analyzed for the most common mitochondrial DNA mutations associated with Leber's hereditary optic neuropathy. None of the participants tested were found to have the mitochondrial DNA mutations at nucleotide positions 11778, 3460, 14484, 7444, or 9804. Of 57 definite case subjects and 69 normal control subjects, three case and three control subjects had the mutation at nucleotide position 9438, three different case and three different control subjects had the mutation at position 13708, and one case and one control subject had the mutation at position 15257 in association with the mutation at position 13708. The most common mitochondrial DNA mutations associated with Leber's hereditary optic neuropathy do not appear to be contributing factors in the epidemic neuropathy in Cuba. We also identified a large Cuban family with maternally related members who experienced visual loss consistent with the diagnosis of Leber's hereditary optic neuropathy. Maternal family members harbored the highly pathogenetic mutation at nucleotide position 11778.
Assuntos
Surtos de Doenças , Atrofias Ópticas Hereditárias/genética , Doenças do Nervo Óptico/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Cuba/epidemiologia , DNA Mitocondrial , Eletroforese em Gel de Ágar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mutação , Atrofias Ópticas Hereditárias/epidemiologia , Atrofias Ópticas Hereditárias/etiologia , Doenças do Nervo Óptico/etiologia , Doenças do Nervo Óptico/genética , Linhagem , Reação em Cadeia da PolimeraseRESUMO
An inhibition ELISA technic to detect antibodies aimed at Coxsackie B viruses was standardized. The method was type-specific since it could detect antibodies to 4 Coxsackie B serotypes (it wasn't tried on serotypes B2 and B4 because the strains were not available). The comparison with the neutralization technic showed 85% coincidence, 91% sensitivity and 82% specificity. All the reactives used in the assay were produced in our laboratory.
Assuntos
Infecções por Coxsackievirus/diagnóstico , Enterovirus Humano B , Ensaio de Imunoadsorção Enzimática/normas , Ensaio de Imunoadsorção Enzimática/métodos , Humanos , Sensibilidade e Especificidade , Testes SorológicosRESUMO
The surveillance of the circulation of the wild poliovirus is an essential element for the eradication of poliomyelitis. The Pan-American Health Organization emphasises on the etiological study of acute flaccid paralysis in order to achieve this aim. The characteristics of our program against this entity allowed to use the presence of antibodies in a sample obtained in February from the infant population born on July during the previous year as criteria to know the circulation of wild poliovirus. In 727 infants studies in the country (5% of the total number of births during July), 99.1% showed no antibodies with a titre equal or greater than 1:10. Low titres found in 7 infants were considered as a remnant from maternal transmission. Results obtained represent a strong evidence of the fact that there is no environmental circulation of poliovirus during the period which had the greatest incidence of the disease.
Assuntos
Anticorpos Antivirais/sangue , Poliomielite/sangue , Poliovirus/imunologia , Animais , Biomarcadores/sangue , Pré-Escolar , Chlorocebus aethiops , Cuba/epidemiologia , Humanos , Imunização Passiva , Poliomielite/epidemiologia , Células VeroRESUMO
The causative agent of an hemorrhagic conjunctivitis outbreak was assessed through the study of 18 conjunctival exudates using the indirect immunofluorescence technique and isolation in cell culture of human embryo fibroblasts. In addition, 395 paired serum samples were studied and Enterovirus 70 was found to be responsible for the outbreak.