Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 15 de 15
Filtrar
Mais filtros











Intervalo de ano de publicação
1.
Curr Issues Mol Biol ; 46(7): 6885-6902, 2024 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-39057053

RESUMO

Pereskia sacharosa Griseb. is a plant used in traditional herbal medicine to treat inflammation. We analyzed the phenolic content of P. sacharosa leaves (EEPs) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and investigated the anti-inflammatory properties of EEPs and its flavonoid fraction (F10) in animal models subjected to acute neuroinflammation induced by bacterial lipopolysaccharide (LPS). Coronal brain sections of C57BL/6JN male mice or Wistar male rats administered with EEPs or F10 before LPS were subjected to in situ hybridization to determine c-fos and CD14 mRNA levels in the hypothalamus or GABAA γ2 mRNA levels in the hippocampus. Theta oscillations were recorded every 6 h in the hippocampus of Wistar rats. In total, five flavonoids and eight phenolic acids were identified and quantified in P. sacharosa leaves. Either EEPs or F10 crossed the blood-brain barrier (BBB) into the brain and reduced the mRNA expression of c-fos, CD14, and GABAA γ2. A decrease in theta oscillation was observed in the hippocampus of the LPS group, while the F10 + LPS group overrode the LPS effect on theta activity. We conclude that the bioactive compounds of P. sacharosa reduce the central response to inflammation, allowing the early return of ambulatory activity and well-being of the animal.

2.
Acta toxicol. argent ; 31(2): 1-1, set. 2023.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1568765

RESUMO

Resumen La marihuana es la droga de abuso de mayor consumo en Chile y el mundo. La forma de consumo más utilizada es fumada como cigarrillos, sin embargo, en el último tiempo se ha masificado el uso de vaporizadores. Estos dispositivos utilizan calor para evaporar la resina que se encuentra al interior de los cartuchos, la cual está compuesta principalmente por delta-9-tetrahidrocannabinol en altas concentraciones y otros aditivos como propilenglicol y acetato de vitamina E. En Estados Unidos se ha descrito toxicidad letal asociado al uso de estos dispositivos. En Chile no se conocen intoxicaciones asociadas a estos productos, sin embargo, su uso ha aumentado en los últimos años por lo que es importante conocer cuál es su composición química. El objetivo de este trabajo fue determinar la concentración de los principales compuestos presentes en los cartuchos para vaporizar que son incautados en nuestro país y para esto se im-plementó una metodología analítica para cuantificar los cannabinoides delta-9-tetrahidrocannabinol (THC), cannabidiol (CBD), canna-binol (CBN), y acetato de vitamina E mediante la técnica cromatografía gaseosa con detector de espectrometría de masas (GC/MSD). Se analizaron 51 muestras de cartuchos con una concentración promedio de THC de 60,2% (p/p). Las concentraciones de CBD y CBN no superaron el 2% (p/p) y en el caso de acetato de vitamina E fue detectado en 9 muestras con una concentración promedio de 51,4% (p/p).


Abstract Marijuana is the most widely used drug of abuse in Chile and the world. The most common form of consumption is smoking cigarettes, but more recently the use of vaporizers has become widespread. These devices use heat to vaporize the resin in the cartridges, which consists mainly of THC in high concentrations and other additives such as propylene glycol and vitamin E ace-tate. Lethal toxicity associated with the use of these devices has been described in the United States. In Chile, there are no known poisonings associated with these products; however, their use has increased in recent years, so it is important to know their chem-ical composition. The aim of this study was to determine the concentration of the main compounds present in the vaporizer car-tridges seized in our country. To this end, an analytical methodology was implemented to quantify the cannabinoids delta-9-tetra-hydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN), and vitamin E acetate using the GC/MSD technique. Fifty-one cartridge samples were analyzed with an average THC concentration of 60.2% (w/w). The concentrations of CBD and CBN did not exceed 2% (w/w) and in the case of vitamin E acetate it was detected in 9 samples with an average concentration of 51.4% (w/w).

3.
Front Med (Lausanne) ; 9: 978795, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36250085

RESUMO

Introduction: Knowing a disease is crucial for being able to fight it, especially in a region in which COVID-19 caused so many deaths, such as Latin America. Objective: To determine the association between basic knowledge of COVID-19 and education level according to country of residence in Latin America. Methodology: This is an analytical cross-sectional study. Basic level of knowledge was measured through nine close-ended questions (scale validated in Peru). The score obtained was analyzed through performing a crosstab vs. gender, age, education level, and country of residence. Results: Of a total of 9,222 respondents, almost all of them knew the common symptoms (99%), modes of transmission (93%), and knew how to recognize which was not a specific symptom (93%). Through the multivariate model, we found that there was no association with gender (p = 0.716) or age (p = 0.059), in comparison with those who had primary or a lower education level. All the other higher education levels had statistically significant scores (all p-values p < 0.001). When comparing knowledge according to countries, and using Peru as reference for comparison, Chile, Paraguay, Mexico, Bolivia, Panama, Ecuador, Costa Rica, and Colombia had a better level of knowledge (all p-values < 0.001); however, only El Salvador had a lower level (p < 0.001). Discussion: There was lack of knowledge of some topics, difference according to academic degree and country. As Peru was one of countries that obtained the lowest level of knowledge, it could have influenced the fact that it was the most affected country in the world.

4.
Ginecol. obstet. Méx ; Ginecol. obstet. Méx;90(10): 833-843, ene. 2022. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1430407

RESUMO

Resumen OBJETIVO: Describir la razón de mortalidad materna para 25 regiones peruanas entre 2015 y 2019, conocer su tendencia y algunos factores determinantes. MATERIALES Y MÉTODOS: Estudio retrospectivo y observacional efectuado con base en los casos de muerte materna registrados en el Repositorio Único Nacional de Información de Salud (REUNIS) del Perú de 2015 a 2019. Los datos se analizaron mediante la razón de mortalidad materna por 100,000 nacidos vivos. RESULTADOS: Se observó una tendencia de mortalidad materna descendente (415 casos en 2015 a 302, en 2019) y una razón de mortalidad materna de 76.34. La edad media fue 29.2 años (± 7.8). Las razones de mortalidad materna más altas se observaron en mujeres de la selva peruana (195.1), con parto domiciliario (1754.8) y atendidas en instituciones del primer nivel de atención (113.5). La región con más casos de muerte materna fue la Sierra peruana (37.61%). CONCLUSIÓN: En el periodo de estudio la razón de mortalidad materna en el Perú tuvo una ligera disminución, con una tendencia descendente. Solo 9 regiones lograron una razón de mortalidad materna menor de 70.


Abstract OBJECTIVE: Describe the trend and regional distribution of maternal mortality in Peru between 2015 and 2019. MATERIALS AND METHODS: A retrospective observational study was carried out based on the cases of maternal death (MM) registered in the National Repository of Health Information (REUNIS) of Peru between 2015 and 2019. The data were analyzed using the maternal mortality ratio (MMR) per 100,000 live births (NV). RESULTS: A descending maternal mortality trend was observed (415 cases in 2015 to 302 in 2019) and a MMR of 76.34. The mean age was 29.2 years (± 7.8). The highest MMRs were observed in women from the Peruvian jungle (195.1), with home birth (1754.8), and attended in institutions of the first level of care (113.5). The region with the most cases of MM was the Peruvian Sierra (37.61%). CONCLUSION: In the study period, the MMR in Peru had a slight decrease, with a downward trend. Only 9 regions achieved an MMR <70.

5.
Ginecol. obstet. Méx ; Ginecol. obstet. Méx;90(8): 629-638, ene. 2022. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1404954

RESUMO

Resumen: OBJETIVO: Determinar la frecuencia de embarazos mediante la tasa de nacidos vivos en el grupo etario de 15 a 19 años y su asociación con el Índice de Desarrollo Humano (2016 a 2021). MATERIALES Y MÉTODOS: Estudio observacional, ecológico, exploratorio y correlacional efectuado con base en la información del Sistema de Registro del Certificado de Nacido Vivo en Línea y el Índice de Desarrollo Humano, que son datos por departamento, provincias y municipios. Se incluyeron todas las mujeres residentes en Perú con un recién nacido entre 2016 y 2021. Los datos registrados fueron: total de recién nacidos por departamento, región, provincia y distrito, divididos por grupos etarios de 15 a 19 años. Para el análisis de los datos se utilizaron regresión lineal y correlación de Pearson. RESULTADOS: En el periodo de estudio se registraron 2,843,903 nacimientos de los que 324,654 (11.41%) correspondieron a mujeres menores de 20 años. En el 2017 se registró la más alta cantidad de nacimientos en este grupo etario (n = 58,841). En 2016 el porcentaje más alto de nacimientos de hijos de adolescentes fue de 12.36% y el más bajo (10.51%) se registró en el 2020. CONCLUSIÓN: El porcentaje de adolescentes embarazadas disminuyó levemente en los últimos años, aunque hubo un ligero aumento en el 2021, sobre todo en la región de la selva, que es la de mayor proporción en comparación con las otras. El índice de desarrollo humano provincial y municipal está inversamente relacionado con la proporción de embarazos en adolescentes.


Abstract OBJECTIVE: To determine the frequency of pregnancies through the live birth rate in the 15-19 age group and its association with the Human Development Index (2016 to 2021). MATERIALS AND METHODS: Observational, ecological, exploratory, and correlational study carried out based on information from the Online Live Birth Certificate Registration System and the Human Development Index, which are data by department, provinces and municipalities. All women residing in Peru with a newborn between 2016 and 2021 were included. The data recorded were total newborns by department, region, province and district, divided by age groups from 15 to 19 years. Linear regression and Pearson correlation were used for data analysis. RESULTS: In the study period, 2,843,903 births were registered, of which 324,654 (11.41%) corresponded to women under 20 years of age. In 2017, the highest number of births was recorded in this age group (n = 58,841). In 2016, the highest percentage of births to teenagers was 12.36% and the lowest (10.51%) was recorded in 2020. CONCLUSION: The percentage of pregnant adolescents decreased slightly in recent years, although there was a slight increase in 2021, especially in the jungle region, which has the highest proportion compared to the others. The provincial and municipal human development index is inversely related to the proportion of teenage pregnancy.

6.
Rev Saude Publica ; 54: 52, 2020.
Artigo em Português, Inglês | MEDLINE | ID: mdl-32491113

RESUMO

OBJECTIVE To describe the process of elaboration and validation of the Scale of Perceptions about Alcohol Consumption in Higher Education Students in a Portuguese sample, considering the relationship between alcohol use rates and students' perceptions about the effects of this consumption. METHODS The validation study included 531 Portuguese college freshmen who answered the instrument, which is composed of five items that express positive perceptions and five items that express negative perceptions about the effects of alcohol consumption. RESULTS Evidence of content validity, internal structure and external variables were obtained. The results of the factor analysis confirm the distribution of positive and negative perceptions by two different factors according to the theoretical model. Adequate internal consistency indexes were obtained for each dimension. The data obtained showed expected correlations between the perceptions and consumption behaviors of the students, indicating evidence of criterion validity of the scale. Moreover, the study showed that different consumption patterns between men and women, with higher alcohol consumption in the students' households and restaurants or cafés by male students, in addition to the similarity in the consumption pattern between the two genders in parties and bars or nightclubs. CONCLUSION The data obtained show the validity of the instrument. In the discussion, the article presents considerations about the responsibility of higher education institutions in the prevention and reduction in consumption rates among their students.


Assuntos
Consumo de Álcool na Faculdade/psicologia , Percepção , Estudantes/psicologia , Inquéritos e Questionários/normas , Adolescente , Análise Fatorial , Feminino , Comportamentos de Risco à Saúde/efeitos dos fármacos , Humanos , Masculino , Portugal , Valores de Referência , Reprodutibilidade dos Testes , Fatores Sexuais , Universidades , Adulto Jovem
7.
Rev. saúde pública (Online) ; 54: 52, 2020. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1101867

RESUMO

ABSTRACT OBJECTIVE To describe the process of elaboration and validation of the Scale of Perceptions about Alcohol Consumption in Higher Education Students in a Portuguese sample, considering the relationship between alcohol use rates and students' perceptions about the effects of this consumption. METHODS The validation study included 531 Portuguese college freshmen who answered the instrument, which is composed of five items that express positive perceptions and five items that express negative perceptions about the effects of alcohol consumption. RESULTS Evidence of content validity, internal structure and external variables were obtained. The results of the factor analysis confirm the distribution of positive and negative perceptions by two different factors according to the theoretical model. Adequate internal consistency indexes were obtained for each dimension. The data obtained showed expected correlations between the perceptions and consumption behaviors of the students, indicating evidence of criterion validity of the scale. Moreover, the study showed that different consumption patterns between men and women, with higher alcohol consumption in the students' households and restaurants or cafés by male students, in addition to the similarity in the consumption pattern between the two genders in parties and bars or nightclubs. CONCLUSION The data obtained show the validity of the instrument. In the discussion, the article presents considerations about the responsibility of higher education institutions in the prevention and reduction in consumption rates among their students.


RESUMO OBJETIVO Considerando a relação entre as taxas de uso de álcool e as percepções dos estudantes sobre os efeitos desse consumo, descrever o processo de elaboração e validação da Escala de Percepções sobre o Consumo de Álcool em Estudantes do Ensino Superior em uma amostra portuguesa. MÉTODOS Participaram do estudo de validade 531 estudantes portugueses do primeiro ano do ensino superior que responderam ao instrumento, composto por cinco itens que expressam percepções positivas e cinco itens que expressam percepções negativas sobre os efeitos do consumo de álcool. RESULTADOS Evidências de validade de conteúdo, de estrutura interna e baseadas em variáveis externas foram obtidas. Os resultados da análise fatorial confirmam a distribuição das percepções positivas e negativas por dois fatores diferenciados em acordo com o modelo teórico de partida. Índices adequados de consistência interna foram obtidos para cada dimensão. Os dados obtidos mostraram correlações esperadas entre as percepções e os comportamentos de consumo dos estudantes, indicando evidências de validade de critério da escala. Em acréscimo, o estudo indicou haver padrões de consumo diferenciados entre os sexos, registando-se maior consumo de álcool nas residências e nos restaurantes ou cafés por parte dos homens, além de similaridade entre os dois sexos no padrão de consumo em festas e bares ou discotecas. CONCLUSÃO Os dados obtidos evidenciam a validade do instrumento. Na discussão dos dados, o artigo apresenta considerações sobre a responsabilidade das instituições de ensino superior na prevenção e redução das taxas de consumo entre a sua população estudantil.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Percepção , Estudantes/psicologia , Inquéritos e Questionários/normas , Consumo de Álcool na Faculdade/psicologia , Portugal , Valores de Referência , Universidades , Fatores Sexuais , Reprodutibilidade dos Testes , Análise Fatorial , Comportamentos de Risco à Saúde/efeitos dos fármacos
8.
Probiotics Antimicrob Proteins ; 10(1): 56-63, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-28432676

RESUMO

Rotavirus is the leading worldwide cause of gastroenteritis in children under five years of age. Even though there are some available vaccines to prevent the disease, there are limited strategies for challenging diarrhea induced by rotavirus infection. For this reason, researchers are constantly searching for other approaches to control diarrhea by means of probiotics. In order to demonstrate the ability of some probiotic bacteria to interfere with the in vitro rotavirus infection in MA104 cells, strains of Lactobacillus sp. and Bifidobacterium sp. were tested in MA104 cells before the viral infection. As a preliminary assay, a blocking effect treatment was performed with viable bacteria. In this screening assay, four of initial ten bacteria showed a slight reduction of the viral infection (measured by percentage of infection). L. casei (Lafti L26-DSL), L. fermentum(ATCC 9338), B. adolescentis (DSM 20083), and B. bifidum (ATCC 11863) were used in further experiments. Three different treatments were tested in order to evaluate protein-based metabolites obtained from mentioned bacteria: (i) cell exposure to the protein-based metabolites before viral infection, (ii) exposure to protein-based metabolites after viral infection, and (iii) co-incubation of the virus and protein-based metabolites before viral infection to the cell culture. The best effect performed by protein-based metabolites was observed during the co-incubation assay of the virus and protein-based metabolites before adding them into the cell culture. The results showed 25 and 37% of infection in the presence of L. casei and B. adolescentis respectively. These results suggest that the antiviral effect may be occurring directly with the viral particle instead of making a blocking effect of the cellular receptors that are needed for the viral entrance.


Assuntos
Antivirais/farmacologia , Bifidobacterium adolescentis/fisiologia , Lacticaseibacillus casei/fisiologia , Probióticos/farmacologia , Infecções por Rotavirus/virologia , Rotavirus/efeitos dos fármacos , Ligação Viral/efeitos dos fármacos , Linhagem Celular , Gastroenterite/prevenção & controle , Gastroenterite/virologia , Humanos , Lacticaseibacillus casei/química , Rotavirus/fisiologia , Infecções por Rotavirus/prevenção & controle
9.
Rev. latinoam. psicol ; Rev. latinoam. psicol;46(2): 102-110, mayo-ago. 2014. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-735146

RESUMO

El apoyo social percibido es un constructo multifacético conceptualizado como la valoración cognitiva de que existe una relación de confianza con los otros, con los que se puede contar en caso de necesidad. El objetivo de este trabajo es examinar las propiedades psicométricas de las escalas de apoyo social percibido Social Support Questionnaire-Short Form (SSQ6) y Social Provisions Scale (SPS) en una muestra de 855 universitarios, 575 mujeres y 280 hombres, matriculados en primer año de grado de 16 titulaciones de la Universidad de Santiago de Compostela (España). El SSQ6 mide las dimensiones de disponibilidad y satisfacción del apoyo percibido, mientras que el SPS evalúa sus funciones. Los análisis factoriales realizados confirman el modelo de dos dimensiones del SSQ6 y la estructura de seis factores del SPS. La consistencia interna de ambas escalas es satisfactoria, al igual que las evidencias de validez obtenidas de las correlaciones entre las dos escalas y sus respectivas subescalas. Los resultados revelan que las versiones españolas del SSQ6 y el SPS son medidas fiables y válidas para la evaluación del apoyo social percibido en población universitaria.


Perceived social support is a multifaceted construct conceptualized as one's cognitive appraisal as to the existence of a connection to others, based on trust, on whom one can rely on when necessary. The aim of this paper is to examine the psychometric properties of perceived social support, using the following scales: Social Support Questionnaire-Short Form (SSQ6) and Social Provisions Scale (SPS), on a sample of 855 first year university students - 575 females and 280 males - enrolled in 16 professional degrees at the University of Santiago de Compostela (Spain). The SSQ6 Scale assesses the dimensions of perceived support availability and satisfaction, whilst the SPS Scale assesses its social support functions. The factor analysis conducted confirms the SSQ6 Scale's twodimensional model and the SPS Scale's six-factor structure. Internal consistency of both scales is satisfactory, as is the evidence of validity obtained from the correlations between the two scales and their subscales. The results reveal that the Spanish version of the SSQ6 and SPS scales are reliable and valid measurements for assessing perceived social support amongst university students.

10.
PLoS One ; 6(4): e18888, 2011 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-21559488

RESUMO

BACKGROUND: Viral hepatitis B, C and delta still remain a serious problem worldwide. In Colombia, data from 1980s described that HBV and HDV infection are important causes of hepatitis, but little is known about HCV infection. The aim of this study was to determine the currently frequency of HBV, HCV and HDV in four different Colombian regions. METHODOLOGY/PRINCIPAL FINDINGS: This study was conducted in 697 habitants from 4 Colombian departments: Amazonas, Chocó, Magdalena and San Andres Islands. Epidemiological data were obtained from an interview applied to each individual aiming to evaluate risk factors related to HBV, HCV or HDV infections. All samples were tested for HBsAg, anti-HBc, anti-HBs and anti-HCV markers. Samples that were positive to HBsAg and/or anti-HBc were tested to anti-HDV. Concerning the geographical origin of the samples, the three HBV markers showed a statistically significant difference: HBsAg (p = 0.033) and anti-HBc (p<0.001) were more frequent in Amazonas and Magdalena departments. Isolated anti-HBs (a marker of previous vaccination) frequencies were: Chocó (53.26%), Amazonas (32.88%), Magdalena (17.0%) and San Andrés (15.33%)--p<0.001. Prevalence of anti-HBc increased with age; HBsAg varied from 1.97 to 8.39% (p = 0.033). Amazonas department showed the highest frequency for anti-HCV marker (5.68%), while the lowest frequency was found in San Andrés Island (0.66%). Anti-HDV was found in 9 (5.20%) out of 173 anti-HBc and/or HBsAg positive samples, 8 of them from the Amazonas region and 1 from them Magdalena department. CONCLUSIONS/SIGNIFICANCE: In conclusion, HBV, HCV and HDV infections are detected throughout Colombia in frequency levels that would place some areas as hyperendemic for HBV, especially those found in Amazonas and Magdalena departments. Novel strategies to increase HBV immunization in the rural population and to strengthen HCV surveillance are reinforced by these results.


Assuntos
Hepatite B/epidemiologia , Hepatite B/virologia , Hepatite C/epidemiologia , Hepatite C/virologia , Hepatite D/epidemiologia , Hepatite D/virologia , Adolescente , Adulto , Idoso , Criança , Colômbia/epidemiologia , Feminino , Marcadores Genéticos , Hepacivirus/genética , Vírus da Hepatite B/genética , Vírus Delta da Hepatite/genética , Humanos , Masculino , Pessoa de Meia-Idade , RNA Viral/sangue , Estudos Soroepidemiológicos
11.
Univ. sci ; 16(2): 168-172, 2011. ilus
Artigo em Inglês | LILACS | ID: lil-619186

RESUMO

Identificación de Norovirus Humano (HNoV) en muestras de estiércol de cerdos domésticos. Objetivo: determinar la presencia de NoVs como posible agente zoonótico causal de diarrea aguda entre cerdos y humanos. Materiales y métodos: se recolectaron un total de 77 muestras diarreicas provenientes de niños menores de cinco años y de cerdos menores de dos meses de la población La Chamba en el Tolima, Colombia. Estas muestras fueron transportadas al Laboratorio de Virología de la Pontificia Universidad Javeriana en Bogotá, donde inicialmente se les realizó extracción con Trizol-reagent, siguiendo las instrucciones del fabricante. Una vez obtenido el RNA, el siguiente paso fue hacer la RT-PCR para obtener el producto de amplificacion esperado para NoVs de 213 pb. Finalmente, las muestras positivas obtenidas en la RT-PCR fueron secuenciadas y analizadas mediante métodos bioinformáticos. Resultados: se obtuvieron seis muestras positivas de diarrea de niños y una muestra positiva de diarrea de cerdos, las cuales se evidenciaron en una banda de 231 pb. Cinco de las seis muestras positivas en niños y la muestra positiva en cerdos fueron secuenciadas y analizadas. Conclusiones: dada la estrecha relación genética que se evidencia entre las secuencias del cerdo y el humano, este podría ser un indicio de que exista la posibilidad de un animal en común como reservorio para cepas de humano u otras cepas de animales...


Objective. To determine the presence of NoVs as a possible causal zoonotic agent of acute diarrhea in pigs and humans. Materials and methods. We collected a total of 77 samples from diarrheal children under 5 years and pigs under 2 months from La Chamba town in Tolima, Colombia. These samples were transported to the Laboratory of Virology of the Pontificia Universidad Javeriana in Bogotá, and extraction with Trizol-reagent was done following the manufacturer’s instructions. After obtaining the RNA, the next step was to perform RT-PCR for obtaining the expected amplification product of 213- bp NoVs. Finally, the positive samples obtained in the RT-PCR were sequenced and analyzed by bioinformatics methods. Results. Six positive diarrheic samples from children and a positive diarrheic sample from pigs were detected by a band of 231 bp. Five of the six positive samples in children and the positive pig sample were sequenced and analyzed. Conclusion. Given the close genetic relationship between pig and human sequences, this could be an indication of the potential existence of a common animal acting as a reservoir for human or other animal strains...


Identificação de Norovírus Humano (HNoV) em amostras de suínos domésticos. Objetivo. Determinar a presença de NoVs como possível agente zoonótico causal de diarréia aguda entre porcos e seres humanos. Materiais e métodos. Foram coletadas um total de 77 amostras de crianças diarréicas menores de cinco anos e porcos com menos de dois meses da população “La Chamba” Tolima-Colômbia. Estas amostras foram transportadas ao laboratório de virologia da Pontifícia Universidade Javeriana - Bogotá, onde foram inicialmente submetidas à extração com Trizol reagment e seguindo as instruções do fabricante, após a obtenção do RNA o próximo passo foi realizar a RT-PCR para obter o produto de amplificação esperado para NoVs de 213 bp. Finalmente as amostras positivas obtidas no RT-PCR foram seqüenciadas e analisadas por métodos de bioinformática. Resultados. Foram obtidas seis amostras positivas de diarréia nas crianças e uma amostra positiva de diarréia em suínos, as que foram representadas em uma banda de 231 pb. Cinco das seis amostras positivas em crianças e a amostra positiva em suínos foram seqüenciadas e analisadas. Conclusões. Dada a estreita relação genética que se manifesta entre as seqüências de suínos e humanos, isso poderia ser uma indicação de que existe a possibilidade de um animal comum como reservatório para o humano ou outras cepas de animais...


Assuntos
Diarreia Infantil/virologia , Suínos/virologia , Vírus da Diarreia Epidêmica Suína , Zoonoses/transmissão , Colômbia
12.
Acta amaz ; Acta amaz;38(4): 811-814, dez. 2008. ilus
Artigo em Inglês | LILACS | ID: lil-504717

RESUMO

Paxillus is a neotropical genus belonging to the subfamily Passalinae widespread from Mexico to Argentina. Brazil is known to harbor five species belonging to this genus with most of them presenting at head a flattened central tubercle lacking a free apex. However, one of these species, Paxillus macrocerus Reyes-Castillo & Fonseca, differs from that pattern by having a long central tubercle with a completely free apex. The present study describes and illustrates another new species, which has been found in the Brazilian Amazon, showing this same characteristic.


Paxillus é um gênero neotropical pertencente à subfamília Passalinae, distribuído do México até a Argentina. No Brasil são conhecidas cinco espécies desse gênero, a maioria delas apresentando um tubérculo central achatado sem ápice livre. Entretanto, uma dessas espécies, Paxillus macrocerus Reyes-Castillo & Fonseca, apresenta um padrão diferente, tendo em vista que possui um tubérculo central longo com o ápice completamente livre. O presente estudo descreve e ilustra outra espécie nova, a qual foi encontrada na Amazônia brasileira, que apresenta essa mesma característica.


Assuntos
Besouros , Brasil , Classificação
13.
Artigo em Espanhol | LILACS | ID: lil-758682

RESUMO

Este artículo presenta y fundamenta un Cuestionario realizado dentro de un proyecto inserto en un programa de investigaciones sobre la adolescencia tardía que se desarrolla en la Cátedra I de Adolescencia de Facultad de Psicología de la Universidad de Buenos Aires. El objetivo general de este proyecto es investigar cuáles son los rasgos que caracterizan a los adolescentes tardíos no migrados en la primera subfase de la adolescencia tardía (18 a 21 años) en su tránsito de la escuela secundaria a la universidad. Con el fin de recabar datos relevantes para la investigación se construyó un cuestionario. Para su construcción se han utilizado tres fuentes: Dos Cuestionarios construidos y aplicados en períodos anteriores a este programa de investigación ( Cuestionario sobre Adolescencia Tardía y Cuestionario para Migrados); Datos surgidos de la revisión bibliográfica sobre el ingreso al ámbito universitario de adolescentes; y un análisis de las narrativas sobre el ingreso a la Universidad realizadas en grupos focales con adolescentes de 18 a 21 años...


Assuntos
Humanos , Adolescente , Estudantes/psicologia , Inquéritos e Questionários , Universidades , Universidades
14.
Artigo em Espanhol | LILACS | ID: lil-758767

RESUMO

Este proyecto se incluye en un programa de investigación que desarrolla la Cátedra I de Adolescencia de la Facultad de Psicología de la Universidad de Buenos Aires desde 1992. Durante el período 1992/94, estudiando la adolescencia tardía y sus desenlaces psíquicos, se identificó un subgrupo de características propias: los estudiantes universitarios migrados, provenientes del interior del país. Los individuos de género femenino de este subgrupo fueron objeto de la segunda etapa de este programa de investigación, mediante un proyecto UBACyT 1995/97 (Ps042), y los masculinos lo son del proyecto en curso UBACyT 1998/2000 (TP40).El objetivo del presente proyecto es investigar cuáles son las características particulares en los adolescentes tardíos de la primera subfase (18 a 21 años) con características demográficas de no migrados en el ingreso al ámbito universitario. Además, se busca poder efectuar una comparación entre esta población y la población de estudiantes migrados ya investigada...


Assuntos
Humanos , Estudantes/psicologia , Universidades , Argentina , Psicologia do Adolescente
15.
Artigo em Espanhol | LILACS | ID: biblio-947374

RESUMO

Este proyecto se incluye en un programa de investigación que desarrolla la Cátedra I de Adolescencia de la Facultad de Psicología de la Universidad de Buenos Aires desde 1992. Durante el período 1992/94, estudiando la adolescencia tardía y sus desenlaces psíquicos, se identificó un subgrupo de características propias: los estudiantes universitarios migrados, provenientes del interior del país. Los individuos de género femenino de este subgrupo fueron objeto de la segunda etapa de este programa de investigación, mediante un proyecto UBACyT 1995/97 (Ps042), y los masculinos lo son del proyecto en curso UBACyT 1998/2000 (TP40). El objetivo del presente proyecto es investigar cuáles son las características particulares en los adolescentes tardíos de la primera subfase (18 a 21 años) con características demográficas de no migrados en el ingreso al ámbito universitario. Además, se busca poder efectuar una comparación entre esta población y la población de estudiantes migrados ya investigada.


The current project is included as a part of a research program undertaken since 1992 by the Department number 1 of Adolescence of the School of Psychology at the University of Buenos Aires. During the period 1992/94, studying late adolescence and its psychical outcomes, a subgroup of special characteristics was identified: university students migrating to the capital city from other parts of Argentina. The female subjects of this subgroup were the object of the second part of this research program during 1995/97 under the aid of the UBACyT project Ps042, and the male subjects are the object of the third part,1998/2000, under the aid of the UBACyT project TP40. The purpose of the current project is to research the particular characteristics of late adolescents of the first period (18 to 21 years old) that enter university, who are demographically non migrants. The study intends to make a comparison between this population and that of the migrant students already studied.


Assuntos
Adulto Jovem , Universidades , Adolescente
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA