RESUMO

A genomic clone encoding the Paracoccidioides brasiliensis orotidine monophosphate decarboxylase gene (PbrURA3) was isolated by screening a subgenomic plasmid DNA library of this fungus, using a PCR amplification product of the gene as a probe. Sequence analysis revealed that the gene contains an open reading frame of 855 bp with a single intron (162 bp), and encodes a putative 285 amino acids polypeptide of estimated molecular weight 31.1 kDa and isoelectric point 6.5. The deduced amino acid sequence predicted a 73.4% identity with orotidine monophosphate decarboxylase of Aspergillus nidulans. Functionality of the gene was demonstrated by transformation into a Saccharomyces cerevisiae ura3 null mutant.

Assuntos

Orotidina-5'-Fosfato Descarboxilase/genética , Paracoccidioides/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Biblioteca Gênica , Teste de Complementação Genética , Ponto Isoelétrico , Dados de Sequência Molecular , Peso Molecular , Fases de Leitura Aberta , Paracoccidioides/genética , RNA Fúngico/química , RNA Fúngico/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Transformação Genética