RESUMO
With the advent of antibiotic resistance, pathogenic bacteria have become a major threat in cases of neonatal sepsis; however, guidelines for treatment have not yet been standardized. In this study, 15 cases of neonatal Streptococcus agalactiae sepsis from our hospital were retrospectively analyzed. Of these, nine cases showed early-onset and six cases showed late-onset sepsis. Pathogens were characterized by genotyping and antibiotic sensitivity tests on blood cultures. Results demonstrated that in cases with early-onset sepsis, clinical manifestations affected mainly the respiratory tract, while late-onset sepsis was accompanied by intracranial infection. Therefore, we suggest including a cerebrospinal fluid examination when diagnosing neonatal sepsis. Bacterial genotyping indicated the bacteria were mainly type Ib, Ia, and III S. agalactiae. We recommend treatment with penicillin or ampicillin, since bacteria were resistant to clindamycin and tetracycline. In conclusion, our results provide valuable information for the clinical treatment of S. agalactiae sepsis in neonatal infants.
Assuntos
Bacteriemia/diagnóstico , Doenças do Recém-Nascido/diagnóstico , Infecções Estreptocócicas/diagnóstico , Streptococcus agalactiae , Antibacterianos/uso terapêutico , Bacteriemia/tratamento farmacológico , Bacteriemia/microbiologia , Bacteriemia/fisiopatologia , Feminino , Genótipo , Humanos , Recém-Nascido , Doenças do Recém-Nascido/tratamento farmacológico , Doenças do Recém-Nascido/microbiologia , Masculino , Testes de Sensibilidade Microbiana , Prognóstico , Estudos Retrospectivos , Infecções Estreptocócicas/tratamento farmacológico , Infecções Estreptocócicas/microbiologia , Infecções Estreptocócicas/fisiopatologia , Streptococcus agalactiae/genéticaRESUMO
The aim of this study was to investigate the expression of CD44 and its clinical significance in children suffering from hepatoblastoma (HB). CD44 expression was detected with immunohistochemistry staining in 30 samples from hepatoblastoma children and 10 normal liver tissue samples from normal children. The data obtained was statistically analyzed using the chi-square test, using the SPSS (v.11.0) software. The rate of CD44 expression was significantly higher (66.7%) in hepatoblastoma tissues than in normal liver tissues (χ(2) = 4.848, P < 0.05). The rate of CD44 expression was significantly higher in children with stage III or IV hepatoblastoma (83.3%) than that in children with stage I and II hepatoblastoma (χ(2) ï¼ 5.625, P < 0.05) (41.7%). Therefore, CD44 expression might play an important role in the pathogenesis, progression, and prognosis of HB in children.
Assuntos
Hepatoblastoma/metabolismo , Hepatoblastoma/patologia , Receptores de Hialuronatos/metabolismo , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Criança , Progressão da Doença , Feminino , Expressão Gênica , Hepatoblastoma/genética , Humanos , Receptores de Hialuronatos/genética , Imuno-Histoquímica , Neoplasias Hepáticas/genética , Masculino , Estadiamento de Neoplasias , PrognósticoRESUMO
There are conflicting reports associating the TGF-ß1 gene -C509T polymorphism with susceptibility to IgA nephropathy. We investigated this association through a meta-analysis. Case-control studies were searched up to January 2012; the genotype frequencies in the control group were found to be consistent with Hardy-Weinberg equilibrium. Publication bias was tested by funnel plot and the Egger regression test. Eight studies, comprising 1364 cases and 1483 controls, were included. Significant heterogeneity was observed (χ² = 18.29, P = 0.01). Under the random-effects model, the overall odds ratio (OR) was 1.01 [95% confidence interval (95%CI) = 0.74-1.38; P = 0.94]. In the subgroup analysis based on ethnicities, no significant effect was observed in the Asian descent groups (five comparisons, OR = 0.78; 95%CI = 0.53-1.15; moderate heterogeneity between studies). However, an association was observed in the European descent groups (OR = 1.5; 95%CI = 1.15-1.96; P = 0.003; no significant heterogeneity between studies). There was no evidence of publication bias according to funnel plot and the Egger regression test (a = -2.16, P = 0.23). There was heterogeneity between studies and no clear evidence of an association between the TGF-ß1 gene -C509T polymorphism and susceptibility to IgA nephropathy in the worldwide population. Subgroup analysis suggests that the TGF-ß1 gene -C509T polymorphism would not be a risk factor for IgA nephropathy in Asians but might play a role in Europeans. More studies are required for definitive conclusions.