RESUMO

Biofilms are a bacterial resistance strategy through which microorganisms organize themselves in the form of a colony fixed to a surface that is protected by a polymer matrix. Infectious diseases that result in biofilm formation have been considered a relevant public health problem due to the potential to increase patient morbidity and mortality, in addition to increasing the burden on health services. Such pathologies are treated with the use of antimicrobial drugs, the indiscriminate use of which has contributed to the process of bacterial resistance, demanding the need to invest in new alternatives to combat them. Based on this, the present work aimed to evaluate the anti-biofilm formation and eradication capacity of Hecogenin Acetate, a steroidal sapogenin of natural origin with important antibacterial properties. For this, we used strains of Streptococcus mutans INCQS 00,446 (ATCC 25,175), Enterococcus faecalis INCQS 00,018 (ATCC 14,506), Staphylococcus epidermidis INCQS 00,016 (ATCC 12,228), Staphylococcus aureus ATCC 25,923, Pseudomonas aeruginosa ATCC 9027 and Escherichia coli ATCC 259,223. The formation, formation inhibition and treatment assays were carried out in microdilution plates and revealed using the crystal violet method. Readings were carried out using absorbance at wavelengths of 492 nm. All tests were performed in triplicate and statistical analyzes were performed using Graphpad Prism v.5.0 software. It was observed that the bacterial strains used have a relevant capacity for biofilm formation, with the Gram positive ones identified in the present study as the best former. In the results of the analyzes with bacterial biofilm, it was identified that Hecogenin Acetate had a relevant antibiofilm capacity, and could therefore serve as a basis for further research into the development of new antimicrobial drugs.

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RESUMO

A descoberta de um elo entre a composição da microbiota intestinal, tem levado a avanços significativos na compreensão de condições como a doença de Parkinson. Objetivo: Analisar a correlação entre as bactérias intestinais e a doença de Parkinson. Métodos: Trata-se de uma revisão integrativa da literatura. Para direcionar a pesquisa, adotou-se como pergunta norteadora: "Qual a relação existente entre as bactérias intestinais com a doença de Parkinson?" Para construção da pesquisa, a coleta e análise de dados foi realizada através do Portal da Biblioteca Virtual em Saúde e das bases de dados Medical Literature Analysis and Retrievel System Online via PubMed e Google Acadêmico através dos seguintes Descritores em Ciências da Saúde (DeCS): "Doença de Parkinson", "Microbioma Gastrointestinal" e "Inflamação" combinados entre si pelo operador booleano AND com seus respectivos correspondentes no Mesh Terms. Resultados e Discussão: A relação entre a microbiota intestinal e a doença de Parkinson tem sido objeto de crescente interesse na comunidade científica. Descobertas indicam que a composição da microbiota intestinal pode desempenhar um papel importante no desenvolvimento e progressão desta doença neurodegenerativa. Considerações Finais: A presença de certas bactérias intestinais, como da família Enterobacteriaceae, tem sido associada ao aumento do risco de desenvolver a doença de Parkinson. Essas bactérias podem produzir substâncias tóxicas capazes de desencadear uma resposta inflamatória no cérebro e contribuir para a morte de neurônios dopaminérgicos

The discovery of a link between the composition of the intestinal microbiota has led to significant advances in the understanding of conditions such as Parkinson's disease. Objective: To analyze the correlation between intestinal bacteria and Parkinson's disease. Methods: This is an integrative literature review. To guide the research, the following guiding question was adopted: "What is the relationship between intestinal bacteria and Parkinson's disease?" Data were collected from the Medical Literature Analysis and Retrievel System Online database via PubMed and Google Scholar through the following HealthSciences Descriptors (DeCS): "Parkinson's Disease", "Gastrointestinal Microbiome" and "Inflammation" combined by the Boolean operator AND with their respective counterparts in Mesh Terms. Results and Discussion: The relationship between the gut microbiota and Parkinson's disease has been the subject of increasing interest in the scientific community. Research indicates that the composition of the intestinal microbiota may play an important role in the development and progression of neurodegenerative disease. Final Thoughts: The presence of certain intestinal bacteria, such as the Enterobacteriaceae, has been associated with increased risk of developing Parkinson's disease. These bacteria can produce toxic substances capable of triggering an inflammatory response in the brain and contributing to the death of dopaminergic neurons

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