RESUMO
The changes in the T cell subsets of the Peyer's patches and the thymus were analyzed in BALB/c mice infected with Trypanosoma cruzi, Tulahuén strain. During the acute stage of the infection both lymphoid organs drastically reduced their cellularity. This was mainly due to the decrease in the immature CD4+CD8+ T cell population in the thymus and in both T and B cells in the Peyer's patches. In the acute infection, few Peyer's patches were found and the histological studies revealed a depletion of the thymic-dependent areas, paralleling the decreased number of cells expressing CD4 and alpha beta T cell receptor. After 14 weeks, in the late stage of the infection, the cellularity and the levels of the T cell subsets studied returned to values similar to those of noninfected mice.
Assuntos
Doença de Chagas/imunologia , Nódulos Linfáticos Agregados/imunologia , Subpopulações de Linfócitos T/patologia , Doença Aguda , Animais , Relação CD4-CD8 , Doença de Chagas/patologia , Citometria de Fluxo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Parasitemia/imunologia , Parasitemia/patologia , Nódulos Linfáticos Agregados/patologia , Timo/imunologia , Timo/patologiaRESUMO
A peroxidase-antiperoxidase (PAP) technique was used to diagnose bacillary haemoglobinuria in formalin-fixed, paraffin-embedded liver tissues of cattle. The PAP method revealed Clostridium haemolyticum in the zone of liver necrosis characteristic of the disease and also in culture smears of this microorganism, but C. novyi type B, C. chauvoei, C. septicum and C. perfringens types B and C remained unstained by the PAP reaction. The PAP technique performed provides a specific, simple and rapid method to diagnose bacillary haemoglobinuria.