RESUMO
Objetivo: Determinar elementos traço na saliva de indivíduos com fibrose cística.Métodos: Trinta e cinco indivíduos com fibrose cística e trinta e nove indivíduos saudáveis participaram deste estudo. A distribuição de 24 elementos traço na saliva total estimulada foi avaliada por espectrometria de massa com plasma de argônio acoplado indutivamente (ICP-MS), espectrometria de emissão óptica com plasma de argônio acoplado indutivamente (ICP-OES) e espectrometria de absorção atômica (AAS).Resultados: Verificou-se uma diferença significativa na distribuição de elementos traço na saliva de indivíduos com fibrose cística quando comparado a indivíduos sem a doença. Sódio; Potássio; Vanádio; Cromo; Arsênio e Selênio apresentaram valores estatisticamente diferentes entre os grupos, enquanto Alumínio, Boro, Bário, Cadmo, Cobre, Ferro, Mercúrio, Magnésio, Manganês, Níquel, Titânio, Estrôncio e Zinco não apresentaram diferenças significativas entre os grupos. As análises estatísticas indicaram que os elementos Sódio e Potássio apresentaram maiores teores, enquanto que os elementos traço Vanádio, Cromo, Selênio e Arsênio apresentaram menores teores nos indivíduos com fibrose cística.Conclusões: A espectroscopia atômica mostrou um potencial considerável no desenvolvimento de metodologia destinada a detectar a fibrose cística neonatal. A saliva deve ser considerada como material biológico para análise de diagnóstico
Objective: To determine trace elements in the saliva of individuals with cystic fibrosis.Method: Thirty-five individuals with cystic fibrosis and thirty-nine healthy individuals participated in this study. The distribution of 24 trace elements in stimulated whole saliva was evaluated by inductivelycoupled plasma mass spectrometry (ICP-MS), inductively coupled plasma optical emission spectrometry (ICP-OES) and atomic absorption spectrometry (AAS).Results: There was a statistically significant difference in the distribution of trace elements in the saliva of individuals with cystic fibrosis compared with individuals without the disease. Sodium, potassium, vanadium, chromium, arsenic and selenium presented significantly different values between the groups, while no significant differences was found between the groups for aluminum, boron, barium, cadmium, copper, iron, mercury, magnesium, manganese, nickel, titanium, strontium and zinc. Statistical analyses indicated higher contents of the trace elements sodium and potassium and lower contents of the trace elements vanadium, chromium, selenium and arsenic in the individuals with cystic fibrosis.Conclusion: Atomic spectroscopy showed a considerable potential in the development of a methodology to detect a neonatal cystic fibrosis. The saliva should be considered as biological material for diagnostic analysis
Assuntos
Humanos , Masculino , Feminino , Espectrometria de Massas/métodos , Fibrose Cística/diagnóstico , Saliva/química , Análise de VariânciaRESUMO
PURPOSES: Cystic fibrosis (CF) is an autosomal recessive hereditary disease and is the frequently common lethal genetic pathology. The purposes of this study were to: (1) determine the presence of 3 different types of enamel defects: (a) demarcated opacities; (b) diffuse opacities; and (c) hypoplasia in the deciduous and permanent dentition of CF patients; and (2) compare with a control group. METHODS: The case group was defined as 13 patients who were diagnosed with CF and enrolled in a multiprofessional project of the Catholic University of Brasília (CUB), Brasilia, Brazil. All CF subjects were compared with control subjects selected from patients at the CUB. Each CF subject was individually paired with a control subject of similar sex and age. A full-mouth examination was carried out for the developmental defects of enamel (DDE) index. RESULTS: The most prevalent enamel defect in deciduous teeth was demarcated opacities present in 16% of the case group and in 7% of the control group. Although the defects were more prevalent in the case group, the difference was not statistically significant (P=0.57). The frequency of demarcated opacities was more prevalent in permanent teeth of the case group: 39% compared to 11% in the control group. For the control group, diffuse opacities were the more prevalent defects: 17% compared to 15% in the case group. The case group had more enamel defects in permanent teeth compared to the control (P=0.0003). CONCLUSIONS: In this study, enamel defects were frequently found in the permanent teeth of CF patients. Therefore, professionals who treat children should be alerted to promoting oral health among these patients.
Assuntos
Fibrose Cística/complicações , Esmalte Dentário/anormalidades , Estudos de Casos e Controles , Criança , Esmalte Dentário/patologia , Hipoplasia do Esmalte Dentário/complicações , Hipoplasia do Esmalte Dentário/patologia , Feminino , Humanos , Masculino , Dente/patologia , Dente Decíduo/anormalidades , Dente Decíduo/patologiaRESUMO
Insetos hematófagos transmitem doenças ao contaminar a presa durante o repasto. Acredita-se que um longo processo de evolução teria feito com que tais insetos acumulassem na secreção salivar uma gama de compostos que facilitariam a obtenção do sangue...