RESUMO
This study evaluated the ability of three materials--a resinous root canal sealer (Sealer 26) prepared in a thick consistence, a reinforced zinc oxide-eugenol cement (IRM), and a glass-ionomer cement (Fuji IX)--in preventing bacterial leakage. Retrofilled teeth were mounted in an apparatus and then challenged by human saliva. The number of days required for the bacteria from saliva to penetrate the root-end filling materials was determined. Evaluation was conducted for 60 days. Leakage was observed in all teeth of the Fuji IX group, and in 95% (19 of 20 specimens) of the teeth retrofilled with IRM. Sixty-five percent (13 of 20 teeth) of the teeth retrofilled with Sealer 26 showed leakage. No difference was detected between Fuji IX and IRM (p > 0.05). However Sealer 26 was significantly more effective in preventing bacterial leakage when compared with other materials tested (p < 0.05).
Assuntos
Bactérias/efeitos dos fármacos , Cimentos Dentários/uso terapêutico , Infiltração Dentária/prevenção & controle , Obturação Retrógrada/métodos , Materiais Restauradores do Canal Radicular/uso terapêutico , Bismuto/química , Bismuto/uso terapêutico , Hidróxido de Cálcio/química , Hidróxido de Cálcio/uso terapêutico , Cimentos Dentários/química , Infiltração Dentária/microbiologia , Cavidade Pulpar/anatomia & histologia , Cavidade Pulpar/diagnóstico por imagem , Cimentos de Ionômeros de Vidro/química , Cimentos de Ionômeros de Vidro/uso terapêutico , Humanos , Metilmetacrilatos/química , Metilmetacrilatos/uso terapêutico , Radiografia , Materiais Restauradores do Canal Radicular/química , Preparo de Canal Radicular/métodos , Saliva/microbiologia , Propriedades de Superfície , Fatores de Tempo , Cimento de Óxido de Zinco e Eugenol/química , Cimento de Óxido de Zinco e Eugenol/uso terapêuticoRESUMO
AIM: A 16S rDNA-based polymerase chain reaction (PCR) method was used to detect the occurrence of Treponema denticola in root canals. METHODOLOGY: Samples were collected from 54 single-rooted teeth having carious lesions and necrotic pulps. DNA extracted from the samples was amplified using the PCR assay, which yielded a specific fragment of T. denticola 16S rDNA. RESULTS: T. denticola was detected in 10 of 29 asymptomatic cases (34.5%), eight of 15 root canals that were tender to percussion (53.3%), and five of 10 teeth with acute periradicular abscess (50%). In general, T. denticola was found in 23 of 54 cases (42.6%). CONCLUSIONS: Findings suggest that T. denticola can be involved in the pathogenesis of periradicular lesions of endodontic origin.
Assuntos
Cavidade Pulpar/microbiologia , Necrose da Polpa Dentária/microbiologia , Treponema/classificação , Infecções por Treponema/diagnóstico , Adolescente , Adulto , Perda do Osso Alveolar/microbiologia , Primers do DNA , DNA Bacteriano , Cárie Dentária/microbiologia , Eletroforese em Gel de Ágar , Amplificação de Genes , Humanos , Pessoa de Meia-Idade , Abscesso Periapical/microbiologia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S , Treponema/genéticaRESUMO
OBJECTIVE: The purpose of this in vitro study was to evaluate the coronal leakage of microorganisms from saliva into root canals filled by 3 obturation techniques. STUDY DESIGN: Obturated canals were mounted in an apparatus and then exposed to human saliva. The number of days required for the entire contamination of the root canal was recorded. Evaluation was carried out for 60 days. RESULTS: Regardless of the technique used, a significant number of specimens were contaminated after 30 and 60 days. No significant differences were detected among the techniques (P >.05). CONCLUSIONS: Results indicated that none of the techniques tested could predictably produce a coronal bacteria-tight seal of the root canal after direct saliva challenge.
Assuntos
Infiltração Dentária , Obturação do Canal Radicular/métodos , Infiltração Dentária/prevenção & controle , Humanos , Técnicas In Vitro , Incisivo , Saliva/microbiologia , Fatores de TempoRESUMO
Endodontic sealers that possess both optimum flow ability and antimicrobial properties may theoretically assist in the elimination of microorganisms located in confined areas of the root canal system. The antimicrobial effects and the flow rate of the following sealers were investigated and compared: Kerr Pulp Canal Sealer EWT, Grossman's Sealer, ThermaSeal, Sealer 26, AH Plus, and Sealer Plus. The agar diffusion test was used to assess the antimicrobial activity of the sealers. In the flow assay, the sealers were placed between two glass slabs and a weight of 500 g was placed on the top of the glass. The diameters of the formed discs were recorded. All root canal sealers tested showed some antimicrobial activity against most of the microorganisms. There were no significant differences between the materials tested (p > 0.05). All root canal sealers also flowed under the conditions of this study. Statistical analysis of the results revealed that AH Plus and Kerr Pulp Canal Sealer EWT had flow values significantly superior to the other sealers tested (p > 0.05). Taken together, these findings suggest that these sealers have the potential to help in the microbial control in the root canal system.
Assuntos
Anti-Infecciosos/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Ágar , Análise de Variância , Antibacterianos , Anti-Infecciosos/química , Bactérias/efeitos dos fármacos , Bismuto/química , Bismuto/farmacologia , Hidróxido de Cálcio/química , Hidróxido de Cálcio/farmacologia , Candida albicans/efeitos dos fármacos , Cavidade Pulpar/microbiologia , Enterococcus faecalis/efeitos dos fármacos , Resinas Epóxi/química , Resinas Epóxi/farmacologia , Escherichia coli/efeitos dos fármacos , Humanos , Imunodifusão , Lacticaseibacillus casei/efeitos dos fármacos , Teste de Materiais , Porphyromonas gingivalis/efeitos dos fármacos , Prevotella/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Reologia , Materiais Restauradores do Canal Radicular/química , Estatísticas não Paramétricas , Streptococcus/efeitos dos fármacos , Cimento de Óxido de Zinco e Eugenol/química , Cimento de Óxido de Zinco e Eugenol/farmacologiaRESUMO
A 16S rDNA-based polymerase chain reaction (PCR) method was used to detect the occurrence of Treponema denticola in root canal infections. Samples were collected from 21 single-root teeth having carious lesions, necrotic pulps and radiographic evidences of periradicular bone loss. DNA extracted from the samples was amplified using the PCR assay, which yielded specific fragment of T. denticola 16S rDNA. T. denticola was detected in 11 of 21 cases (52.4%), regardless of the presence or absence of symptoms. Since this spirochete was found in a relatively high percentage of the endodontic infections examined and because it is a pathogenic microorganism involved in periodontal diseases, there are reasons to believe that T. denticola can also participate in the pathogenesis of periradicular lesions of endodontic origin.
Assuntos
Doenças da Polpa Dentária/microbiologia , Treponema/classificação , Infecções por Treponema/diagnóstico , Adulto , Perda do Osso Alveolar/microbiologia , DNA Bacteriano/análise , DNA Bacteriano/genética , Cárie Dentária/microbiologia , Necrose da Polpa Dentária/microbiologia , Genes Bacterianos/genética , Humanos , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/análise , RNA Ribossômico 16S/genética , Treponema/crescimento & desenvolvimentoRESUMO
Given the importance of bacteria in the development of periradicular lesions, the eradication of the root canal infection is paramount in endodontic treatment. This study evaluated the in vitro intracanal bacterial reduction produced by instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite (NaOCl) or saline solution. Root canals inoculated with Enterococcus faecalis were instrumented and irrigated with the solutions tested. Canals were sampled before and after preparation. After serial dilution, samples were plated onto Mitis salivarius agar, and the colony-forming units grown were counted. Inhibitory effects of the three NaOCl solutions on E. faecalis were also evaluated by means of the agar diffusion test. All test solutions significantly reduced the number of bacterial cells in the root canal (p < 0.05). There was no significant difference between the three NaOCl solutions tested (p > 0.05). Nonetheless, all NaOCl solutions were significantly more effective than saline solution in reducing the number of bacterial cells within the root canal (p < 0.05). The three NaOCl concentrations showed large zones of inhibition against E. faecalis. The results of this study suggest that regular exchange and the use of large amounts of irrigant should maintain the antibacterial effectiveness of the NaOCl solution, compensating for the effects of concentration.