RESUMO
OBJECTIVES: The purpose of the study is to investigate the roles of miR-448 in ovarian cancer. METHODS: miR-448 and CXCL12 mRNA expression were examined using qRT-PCR. CXCL12 promoter activity was detected by luciferase activity system. Cell proliferation was assayed by MTT or colony formation. Migration and invasion was assayed by transwell chamber. RESULTS: miR-448 expression was usually under-expressed in ovarian cancer tissues and cell lines compared with their normal ones. Ectopic expression of miR-448 inhibited cell proliferation, migration and invasion in ovarian cancer cells. Moreover, bioinformatic prediction suggested that CXCL12 was a target gene of miR-448. We also demonstrated that restored expression of CXCL12 dampened miR-448-mediated suppression of tumor progression, which suggests the important role of miR-448 in tumor progression. CONCLUSION: Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12.
Assuntos
Proliferação de Células , Quimiocina CXCL12/biossíntese , Regulação Neoplásica da Expressão Gênica/genética , MicroRNAs/genética , Invasividade Neoplásica/genética , Neoplasias Ovarianas/genética , Western Blotting , Proliferação de Células/genética , Feminino , Genes Supressores de Tumor , Humanos , Neoplasias Ovarianas/patologia , RNA Interferente Pequeno , Reação em Cadeia da Polimerase em Tempo Real , Transdução GenéticaRESUMO
Keshan disease (KSD), a potentially fatal cardiomyopathy, has very high incidence in some selenium-poor regions of China. KSD may be accompanied with a variety of arrhythmia, which is associated with mutations in the gene coding for cardiac voltage-gated sodium channel (SCN5A). The molecular mechanism of KSD is still largely obscure. We aimed to determine the association between the H558R polymorphism of SCN5A and KSD. We recruited 71 patients with KSD and 80 geographical region-matched control subjects in our study. Vital sign and electrocardiographic (ECG) measurements were performed for heart rate, systolic pressure, diastolic pressure, PR interval, QT interval, QRS duration, ST-T changes and complete right bundle branch block (CRBBB), and H558R polymorphism was genotyped using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method and sequencing. A significant association was found between the H558R polymorphism of exon 12 and KSD. Allele C carriers had a decreased risk for KSD with an odds ratio of 0.332 [95% confidence interval (CI), 0.160-0.692] as well as for QRS prolongation in KSD patients with an odds ratio of 0.089 (95%CI, 0.022-0.361). Our results provide support to the association between H558R polymorphism and the decreased risk for KSD. H558R polymorphism might increase susceptibility to KSD, and SCN5A containing the polymorphism might be a predisposing gene for QRS prolongation.
Assuntos
Cardiomiopatias/genética , Infecções por Enterovirus/genética , Predisposição Genética para Doença/genética , Canal de Sódio Disparado por Voltagem NAV1.5/genética , Polimorfismo Genético , Idoso , Pressão Sanguínea , Cardiomiopatias/fisiopatologia , China , Estudos de Coortes , Análise Mutacional de DNA/métodos , Eletrocardiografia , Infecções por Enterovirus/fisiopatologia , Éxons/genética , Feminino , Frequência do Gene , Genótipo , Frequência Cardíaca , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , Fatores de RiscoRESUMO
Headache can be attributed to cranial or cervical vascular disorders including ischemic stroke or transient ischemic attack, non-traumatic intracranial hemorrhage, unruptured vascular malformation, arteritis, carotid-vertebral artery pain, and cerebral venous thrombosis. Here, we present a case report of unruptured saccular aneurysm with migraine. The patient was a previously healthy 32-year-old man with repeated episodes of headache for 6 years. Findings for computed tomography and magnetic resonance imaging of the head were normal. Head magnetic resonance angiography revealed a small, nodule-like protuberance seen in the anterior communicating artery. Based on his clinical features and ancillary examinations, the patient was diagnosed with migraine without aura. The patient subsequently underwent digital subtraction angiography (DSA), which revealed a 2-mm cystic protuberance in the superoposterior anterior communicating artery. The patient underwent stent-assisted coil embolization of the aneurysm. Subsequent DSA results indicated no recurrence of aneurysm and no recurrence of headache was reported after surgical treatment.
Assuntos
Aneurisma/diagnóstico por imagem , Aneurisma Intracraniano/diagnóstico por imagem , Transtornos de Enxaqueca/diagnóstico por imagem , Stents , Adulto , Aneurisma/patologia , Aneurisma/terapia , Angiografia Digital , Humanos , Aneurisma Intracraniano/patologia , Imageamento por Ressonância Magnética , Masculino , Transtornos de Enxaqueca/patologia , Transtornos de Enxaqueca/terapia , Tomografia Computadorizada por Raios XRESUMO
We identified a disease-causing mutation of the RUNX2 gene in a four-generation Chinese family affected with cleidocranial dysplasia (CCD). For mutation analysis, the coding region of RUNX2 was sequenced with DNA from two patients and three unaffected family members. The RUNX2 mutation was investigated in 50 normal controls by denaturing high pressure liquid chromatography. A heterozygous single-base deletion (c.549delC) of RUNX2, which predicts a termination site at the 185th codon and leads to a stop in the runt domain of RUNX2 protein, was detected in both patients but not in the three unaffected members of the family. This mutation was also not found in 50 controls and has not been reported previously. We demonstrated that a novel mutation (c.549delC) of RUNX2 is associated with CCD in a Chinese family, adding to the repertoire of RUNX2 mutations related to CCD.
Assuntos
Povo Asiático/genética , Displasia Cleidocraniana/genética , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Deleção de Sequência/genética , Adolescente , Sequência de Aminoácidos , Sequência de Bases , China , Displasia Cleidocraniana/diagnóstico por imagem , Subunidade alfa 1 de Fator de Ligação ao Core/química , Análise Mutacional de DNA , Família , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Dados de Sequência Molecular , Linhagem , Fenótipo , RadiografiaRESUMO
The outcome of Leishmania infections is determined by both the parasite species and the host genetic makeup. While much has been learned regarding immune responses to this parasite, our knowledge on parasite-derived factors is limited. The recent completion of the L. major and L. infantum genome sequence projects and concurrent advancement in proteomics technology would greatly accelerate the search for novel Leishmania proteins. Using a proteomics-based approach to study species-specific Leishmania proteins, we developed high-resolution, broad pH (3-10) two-dimensional gel electrophoresis (2-DE) separations to determine protein-expression profiles between highly infectious forms of the parasitic species L. amazonensis (New World) and L. major (Old World). Approximately 1,650 and 1,530 distinct protein spots were detected in the L. amazonensis and L. major gels, respectively. While a vast majority of the spots had similar distribution and intensity, a few were computationally defined as preferentially expressed in L. amazonensis in comparison to L. major, or vice versa. These data attest to the feasibility of establishing a 2-DE-based protein array for inter-species profiling of Leishmania proteins and provide the framework for future design of proteome studies of Leishmania.
Assuntos
Eletroforese em Gel Bidimensional/métodos , Leishmania major/química , Leishmania mexicana/química , Proteoma/análise , Proteínas de Protozoários/análise , Animais , Estudos de Viabilidade , Regulação da Expressão Gênica , Leishmania major/genética , Leishmania mexicana/genética , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos BALB C , Proteômica/métodosRESUMO
The outcome of Leishmania infections is determined by both the parasite species and the host genetic makeup. While much has been learned regarding immune responses to this parasite, our knowledge on parasite-derived factors is limited. The recent completion of the L. major and L. infantum genome sequence projects and concurrent advancement in proteomics technology would greatly accelerate the search for novel Leishmania proteins. Using a proteomics-based approach to study species-specific Leishmania proteins, we developed high-resolution, broad pH (3-10) two-dimensional gel electrophoresis (2-DE) separations to determine protein-expression profiles between highly infectious forms of the parasitic species L. amazonensis (New World) and L. major (Old World). Approximately 1,650 and 1,530 distinct protein spots were detected in the L. amazonensis and L. major gels, respectively. While a vast majority of the spots had similar distribution and intensity, a few were computationally defined as preferentially expressed in L. amazonensis in comparison to L. major, or vice versa. These data attest to the feasibility of establishing a 2-DE-based protein array for inter-species profiling of Leishmania proteins and provide the framework for future design of proteome studies of Leishmania.
Assuntos
Animais , Camundongos , Eletroforese em Gel Bidimensional/métodos , Leishmania major/química , Leishmania mexicana/química , Proteoma/análise , Proteínas de Protozoários/análise , Estudos de Viabilidade , Regulação da Expressão Gênica , Leishmania major/genética , Leishmania mexicana/genética , Espectrometria de Massas , Camundongos Endogâmicos BALB C , Proteômica/métodosRESUMO
The healing of colorectal anastomoses after irradiation therapy continues to be a major concern. The authors evaluated the healing of rectal anastomoses in a rat model after a preoperative 500-cGy dose of cobalt60 irradiation. Thirty-six male Wistar rats were divided into two equal groups: control (group A), and irradiation group (group B). Group B received a single 500-cGy dose of irradiation, and a rectal resection and end-to-end anastomosis was performed in both groups on the 7th day after irradiation. Parameters of the healing process included bursting pressure and collagen content on the 5th, 7th, and 14th days after surgery. In the irradiation group, the mean bursting pressure on the 5th, 7th, and 14th days was 116, 218, and 273 mmHg, respectively. The collagen content assessed by histomorphometry was 9.0, 20.8, and 32%, respectively. In contrast, the control group had a mean bursting pressure of 175, 225 and 263 mmHg, and a collagen content of 17.8, 28.1, and 32.1%, respectively. The adverse effect of irradiation on healing was detectable only on the 5th postoperative day, as demonstrated by lower bursting pressure (P < 0.013) and collagen content (P < 0.008). However, there was no failure of anastomotic healing such as leakage or dehiscence due to irradiation. We conclude that a single preoperative 500-cGy dose of irradiation delays the healing of rectal anastomosis in rats.
Assuntos
Isótopos do Cobalto/efeitos adversos , Colo/cirurgia , Reto/cirurgia , Cicatrização/efeitos da radiação , Anastomose Cirúrgica , Animais , Isótopos do Cobalto/uso terapêutico , Masculino , Cuidados Pré-Operatórios , Doses de Radiação , Distribuição Aleatória , Ratos , Ratos Wistar , Fatores de TempoRESUMO
The healing of colorectal anastomoses after irradiation therapy continues to be a major concern. The authors evaluated the healing of rectal anastomoses in a rat model after a preoperative 500-cGy dose of cobalt60 irradiation. Thirty-six male Wistar rats were divided into two equal groups: control (group A), and irradiation group (group B). Group B received a single 500-cGy dose of irradiation, and a rectal resection and end-to-end anastomosis was performed in both groups on the 7th day after irradiation. Parameters of the healing process included bursting pressure and collagen content on the 5th, 7th, and 14th days after surgery. In the irradiation group, the mean bursting pressure on the 5th, 7th, and 14th days was 116, 218, and 273 mmHg, respectively. The collagen content assessed by histomorphometry was 9.0, 20.8, and 32 percent, respectively. In contrast, the control group had a mean bursting pressure of 175, 225 and 263 mmHg, and a collagen content of 17.8, 28.1, and 32.1 percent, respectively. The adverse effect of irradiation on healing was detectable only on the 5th postoperative day, as demonstrated by lower bursting pressure (P < 0.013) and collagen content (P < 0.008). However, there was no failure of anastomotic healing such as leakage or dehiscence due to irradiation. We conclude that a single preoperative 500-cGy dose of irradiation delays the healing of rectal anastomosis in rats.
Assuntos
Animais , Masculino , Ratos , Isótopos do Cobalto/efeitos adversos , Colo/cirurgia , Reto/cirurgia , Cicatrização/efeitos da radiação , Anastomose Cirúrgica , Isótopos do Cobalto/uso terapêutico , Cuidados Pré-Operatórios , Doses de Radiação , Distribuição Aleatória , Ratos Wistar , Fatores de TempoRESUMO
BACKGROUND: Currently, human T-lymphotropic virus type II (HTLV-II) is the most prevalent human retrovirus, detected in persons presenting to donate blood in the United States. Only scant information is available with which to counsel HTLV-II-seropositive deferred donors or other persons about the ways in which they may spread HTLV-II to others. STUDY DESIGN AND METHODS: To increase understanding of the modes of transmission of HTLV-II, a seroepidemiologic study was conducted among Panamanian Guaymi Indians, a recently identified focus of endemic HTLV-II infection. Subjects were tested for serologic evidence of infection by HTLV-II, HTLV type I, hepatitis B virus, and nine other infectious agents by enzyme immunoassays and specific confirmatory tests. RESULTS: Nine (8.3%) of 109 persons tested HTLV-II-seropositive. HTLV-II seropositivity was more likely in persons with serologic evidence of prior hepatitis B virus infection. Sexual contact with HTLV-II-seropositive partners, but neither parenteral exposure nor breast-feeding, was identified as a risk factor for HTLV-II. CONCLUSION: In Guaymi Indians, HTLV-II appears to be spread primarily through sexual transmission.
Assuntos
Infecções por HTLV-II/diagnóstico , Indígenas Centro-Americanos , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Anticorpos Anti-HTLV-II/sangue , Infecções por HTLV-II/etnologia , Infecções por HTLV-II/transmissão , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Panamá , Linhagem , Fatores de Risco , Parceiros SexuaisRESUMO
A total of 1,915 sera collected in 1979 from asymptomatic hepatitis B surface antigen (HBsAg) carriers were tested for delta antigen, antibody to delta antigen (anti-delta), hepatitis B e antigen (HBeAg) and antibody to hepatitis B e antigen (anti-HBe) in addition to HBsAg and its subtypes. These sera represented blood donated by volunteers to 49 of 57 regions of the American Red Cross located in nine geographic regions of the United States and Puerto Rico. A total of 72 (3.8%) sera had anti-delta activity while none had a detectable level of delta antigen. A significantly higher (p less than 0.01) prevalence of anti-delta (12.1%) was found in San Jose, California (Pacific Region); on the other hand, the East South Central region covering Alabama, Kentucky, Mississippi and Tennessee had a significantly lower (p less than 0.05) prevalence (1.4%) of anti-delta when compared with all other regions combined. Anti-delta was, however, detected in all regions of the United States and in Puerto Rico. The cause of significant differences in the prevalence of anti-delta was not clear. The distribution of anti-delta was not associated with age, sex or blood type of the donor. Sixty-nine of 70 samples with anti-delta were found among the 1,527 samples that had either HBeAg or anti-HBe. And among 149 that lacked both HBeAg and anti-HBe, only one sample had anti-delta. The difference is statistically significant (p less than 0.05). The presence of anti-delta was not associated with HBsAg/ad (2.7%) or HBsAg/ay (4.6%).
Assuntos
Doadores de Sangue , Antígenos da Hepatite B/imunologia , Antígenos de Superfície da Hepatite B/imunologia , Antígenos E da Hepatite B/imunologia , Adolescente , Adulto , Métodos Epidemiológicos , Feminino , Antígenos de Superfície da Hepatite B/classificação , Humanos , Masculino , Pessoa de Meia-Idade , Porto Rico , Radioimunoensaio , Estados UnidosRESUMO
Report presenting the results of a study made of Hepatitis B viral markers. A total of 7.487 donor blood samples from 13 Western Hemisphere countries (Argentina, Barbados, Brazil, Chile, Colombia, Costa Rica, the Dominican Republic, Ecuador, Mexico, Peru, Puerto Rico, Suriname, and Venezuela) were tested for various markers of hepatitis B virus (HBV) infection with several different techniques. HBsAg was detected in 1.6 per cent of the samples, anti-HBs in 20.2 per cent, and anti-HBs in 21.3 per cent. The incidence of HBsAg varied from 0.2 per cent (in Puerto Rican samples) to 4.1 per cent (in the samples from the Dominican Republic). Overall, 5.8 per cent of the samples found to contain HBsAg also contained HBeAg, while 24.8 per cent had detectable anti-HBe. Sixty-six (54.5 per cent) of the 121 HBsAg-positive samples were found to contain subtype HBsAg/ad, and 5(4.1 per cent) were found to contain subtype HBsAg/ay. Subtypes of the remaining samples could not be determined because of insufficient antigen. In a comparitive study, reverse passive hemagglutination (RPHA), counterelectrophoresis (CEP), and immunodiffusion (ID) detected by HBsAg in 90.9. 67.2, and 62.0 per cent of the samples previously found positive by radioimmunoassay (RIA) screening. Considerable variation was observed in the prevalence of HBV markers in samples from different countries, the highest prevalence being 82.8 per cent in samples from the Dominican Republic. Over-all, the findings suggest that exposure to HBV is quite extensive in some Latin American populations......(AU)