RESUMO

Fungi are cosmopolitan organisms that grow in and adapt to a vast number of substrates and environments, and that can cause diseases in humans and animals, as well as in crops. The vast area and diverse geographical characteristics of Argentina, with the consequent climatic diversity make the country an important source of biological resources suitable for the search of new compounds. The aim of the present study was to describe the antifungal activity of extracts of Parastrephia quadrangularis, a species from northern Argentina, against Fusarium verticillioides M7075. Bio-guided fractionation and MS/MS studies were conducted to elucidate the chemical structure of active compounds. The extracts exhibited a minimum inhibitory concentration among 118·74 and 250 µg ml-1 and the differences between the treatments and the inoculum control was 12·5-16·5 mm, respectively, in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue, showed alterations in permeability of plasma membranes. HLPC-MS analysis of active fractions revealed the presence of p-coumaroyloxitremetone, and a derivate structure for another compound is proposed. SIGNIFICANCE AND IMPACT OF THE STUDY: In Argentina, Fusarium verticillioides causes 'ear rot', a disease that produces important yield and nutritional quality losses in the maize producing region. This study suggests that Parastrephia quadrangularis extracts have potential for the growth inhibition against F. verticillioides M7075, and the bioactivity is reported for the first time. The results obtained will provide a starting point for discover new antimicotic candidate in natural products.

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RESUMO

Hematologic abnormalities are common in association with collagen diseases, specially Systemic Lupus Erythematosus and include anemia, neutropenia, thrombocytopenia with alterations in lymphocyte subpopulations. On the other hand, patients with unexplained fibrosis of the bone marrow (the syndrome of idiopathic myelofibrosis or primary myelofibrosis) have clinical and laboratory evidence of immunologic dysfunction. Clinical findings include the presence of arthritis, vasculitis and erythema nodosum. Laboratory abnormalities include the presence of circulating immune complexes, antinuclear antibodies, positive direct Coombs test, elevated latex fixation and a circulating lupus type anticoagulant. Total hemolytic complement markedly depressed has also been reported. These data suggest that immunologic mechanisms associated with activation of the complement system play an important role in the disease process of some patients with agnogenic myeloid metaplasia with myelofibrosis. A review of the literature revealed that myelofibrosis occurring in the setting of collagen diseases is rare. However, a role for immunologic factors in the pathogenesis of myelofibrosis is also supported by the patients with coincident well defined collagen disease and myelofibrosis. In this report, we present two patients with such an association. Case 1 was a 58-year-old male with a two year duration history of rheumatic arthritis. He had bone erosions on hands, splenomegaly and myelofibrosis. Rheumatoid factor (latex) was positive: 1:2560. He had positive LE cells and hypocomplementemia: 37 CH50/ml (NV 70-150). The patient did not meet criteria for SLE. Case 2 was a 36-year-old female admitted because of dyspnea and fever. Diagnosis of myeloid metaplasia with myelofibrosis and progressive systemic sclerosis had been made four years before hand.(ABSTRACT TRUNCATED AT 250 WORDS)

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RESUMO

Se revisaron 212 protocolos de pacientes con neoplasias y se hallaron 15 casos de síndromes mieloproliferativos. De éstos, se describen dos pacientes que presentaron una colangenopatía mieloproliferativos. De éstos, se describen dos pacients que ptresentaron una colangenopatía asociada, hecho inusual y poco descripto en la literatura. El caso 1 corresponde a un hombre que presenta una artitis reumatoide clásica de 2 años de evolución con esplenomegalia, factor reumatoide, células LE y FAN positivos e hipocomplementemia. Diagnóstico por biopsia: polictemia vera y mielofibrosis. El caso 2 es una mujer de 36 años, que desde hace 4 años presenta severo fenómeno de Raynaud, esplenomegalia y diagnóstico por biopsia de mielofibrosis. Durante la evolución se fueron constatando elementos de diagnsotico que llegaron a configurar una esclerosis sistémica progresiva e hipertensión pulmonar. Además, presentó factor reumatoide y anti-ADN positivos e hipocomplementemia. Las anormalidades hematológicas son comunes en las colagenopatías y pacientes que presentan mielofibrosis tienen una mayor incidencia de autoanticuerpos, complejos inmunes circulantes e hipocomplementemia. Sin embargo, no es frecuente la asociación de mielofibrosis con colagenopatías

Assuntos

RESUMO

Hematologic abnormalities are common in association with collagen diseases, specially Systemic Lupus Erythematosus and include anemia, neutropenia, thrombocytopenia with alterations in lymphocyte subpopulations. On the other hand, patients with unexplained fibrosis of the bone marrow (the syndrome of idiopathic myelofibrosis or primary myelofibrosis) have clinical and laboratory evidence of immunologic dysfunction. Clinical findings include the presence of arthritis, vasculitis and erythema nodosum. Laboratory abnormalities include the presence of circulating immune complexes, antinuclear antibodies, positive direct Coombs test, elevated latex fixation and a circulating lupus type anticoagulant. Total hemolytic complement markedly depressed has also been reported. These data suggest that immunologic mechanisms associated with activation of the complement system play an important role in the disease process of some patients with agnogenic myeloid metaplasia with myelofibrosis. A review of the literature revealed that myelofibrosis occurring in the setting of collagen diseases is rare. However, a role for immunologic factors in the pathogenesis of myelofibrosis is also supported by the patients with coincident well defined collagen disease and myelofibrosis. In this report, we present two patients with such an association. Case 1 was a 58-year-old male with a two year duration history of rheumatic arthritis. He had bone erosions on hands, splenomegaly and myelofibrosis. Rheumatoid factor (latex) was positive: 1:2560. He had positive LE cells and hypocomplementemia: 37 CH50/ml (NV 70-150). The patient did not meet criteria for SLE. Case 2 was a 36-year-old female admitted because of dyspnea and fever. Diagnosis of myeloid metaplasia with myelofibrosis and progressive systemic sclerosis had been made four years before hand.(ABSTRACT TRUNCATED AT 250 WORDS)

RESUMO

Se revisaron 212 protocolos de pacientes con neoplasias y se hallaron 15 casos de síndromes mieloproliferativos. De éstos, se describen dos pacientes que presentaron una colangenopatía mieloproliferativos. De éstos, se describen dos pacients que ptresentaron una colangenopatía asociada, hecho inusual y poco descripto en la literatura. El caso 1 corresponde a un hombre que presenta una artitis reumatoide clásica de 2 años de evolución con esplenomegalia, factor reumatoide, células LE y FAN positivos e hipocomplementemia. Diagnóstico por biopsia: polictemia vera y mielofibrosis. El caso 2 es una mujer de 36 años, que desde hace 4 años presenta severo fenómeno de Raynaud, esplenomegalia y diagnóstico por biopsia de mielofibrosis. Durante la evolución se fueron constatando elementos de diagnsotico que llegaron a configurar una esclerosis sistémica progresiva e hipertensión pulmonar. Además, presentó factor reumatoide y anti-ADN positivos e hipocomplementemia. Las anormalidades hematológicas son comunes en las colagenopatías y pacientes que presentan mielofibrosis tienen una mayor incidencia de autoanticuerpos, complejos inmunes circulantes e hipocomplementemia. Sin embargo, no es frecuente la asociación de mielofibrosis con colagenopatías (AU)

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