RESUMO
The aim of this work is focused on the extraction and characterization of the Brazilian seaweed Sargassum filipendula alginate. Alginates obtained at different seasons were characterized by liquid state nuclear magnetic resonance spectroscopy and scanning electron microscopy. The alginate extraction efficiency was about 20%. Different seasons of the year and different stages in the life cycle of Sargassum sp. in southeastern Brazil influenced the M/G and, consequently, the technological properties of extracted alginates.
Assuntos
Alginatos/química , Sargassum/química , Alga Marinha/química , Alginatos/isolamento & purificação , Ácido Glucurônico/química , Ácido Glucurônico/isolamento & purificação , Ácidos Hexurônicos/química , Ácidos Hexurônicos/isolamento & purificação , Ressonância Magnética Nuclear Biomolecular , Estações do AnoRESUMO
The isolation, detection and quantification of betulinic acid in Doliocarpus schottianus are described. The isolation from the plant extract was made by column chromatography and centrifugal TLC, and betulinic acid was characterized by spectrometric methods. The detection and quantification were made by HPLC using a C18 column eluted with acetonitrile: water and detected at 210 nm. The results showed that the metabolite accumulates in the bark of the plant, but very small concentrations were also found in the leaves and wood.