RESUMO
Mercury emissions from artisanal and small-scale gold mining throughout the Global South exceed coal combustion as the largest global source of mercury. We examined mercury deposition and storage in an area of the Peruvian Amazon heavily impacted by artisanal gold mining. Intact forests in the Peruvian Amazon near gold mining receive extremely high inputs of mercury and experience elevated total mercury and methylmercury in the atmosphere, canopy foliage, and soils. Here we show for the first time that an intact forest canopy near artisanal gold mining intercepts large amounts of particulate and gaseous mercury, at a rate proportional with total leaf area. We document substantial mercury accumulation in soils, biomass, and resident songbirds in some of the Amazon's most protected and biodiverse areas, raising important questions about how mercury pollution may constrain modern and future conservation efforts in these tropical ecosystems.
Assuntos
Atmosfera/análise , Poluentes Ambientais/análise , Ouro , Mercúrio/análise , Mineração , Floresta Úmida , Animais , Atmosfera/química , Aves/classificação , Aves/metabolismo , Monitoramento Ambiental/métodos , Poluição Ambiental/análise , Plumas/química , Gases/análise , Geografia , Peru , Folhas de Planta/química , Folhas de Planta/metabolismo , Solo/química , Poluentes do Solo/análise , Poluentes do Solo/metabolismoRESUMO
Leptospirosis is a bacterial zoonotic disease with multiple serogroups that infects wildlife and humans alike. Several studies have detected the presence of leptospiral antibodies in captive Neotropical primates, suggesting that they are asymptomatic carriers. However, the presence of antibodies in free-ranging primates and their potential role as reservoirs for this pathogen is not known. In this study, we used the Microscopic Agglutination Test (MAT) to screen two species of free-ranging tamarins for the presence of Leptospira antibodies using a panel of 21 serogroups. A total of 56 primates (26 Leontocebus weddelli and 30 Saguinus imperator) were screened at the Estación Biológica Río Los Amigos in Madre de Dios, Peru. MAT results with titres of ≥100 in single samples were considered seropositive. Prevalence ratios (PR) were estimated for age group, sex and host species identity. Overall, 51.8% (29/56) of animals were reactive for at least one Leptospira serogroup. Serogroups were distributed as follows: Iquitos (41.1%), Icterohaemorrhagiae (14.3%), Sejroe (3.6%), Autumnalis (1.8%) and Bataviae (1.8%). No significant differences (p > .05) were found for seropositivity frequencies among categories within assessed variables. These results suggest that free-ranging L. weddelli and S. imperator might serve as reservoirs for pathogenic Leptospira serogroups. Given increasing human-wildlife contact, future studies that test for active infection are required to determine potential transmission risks to humans.
Assuntos
Leptospira , Leptospirose , Testes de Aglutinação/veterinária , Animais , Animais Selvagens , Anticorpos Antibacterianos , Leptospirose/epidemiologia , Leptospirose/microbiologia , Leptospirose/veterinária , Peru/epidemiologia , Primatas , Floresta Úmida , SorogrupoRESUMO
As biodiversity loss continues to accelerate, there is a critical need for education and biomonitoring across the globe. Portable technologies allow for in situ molecular biodiversity monitoring that has been historically out of reach for many researchers in habitat nations. In the realm of education, portable tools such as DNA sequencers facilitate in situ hands-on training in real-time sequencing and interpretation techniques. Here, we provide step-by-step protocols as a blueprint for a terrestrial conservation genetics field training program that uses low-cost, portable devices to conduct genomics-based training directly in biodiverse habitat countries.
Assuntos
Conservação dos Recursos Naturais/métodos , Genética/educação , Genética/instrumentação , Biodiversidade , Código de Barras de DNA Taxonômico/instrumentação , Código de Barras de DNA Taxonômico/métodos , Ecossistema , Feminino , Genética/organização & administração , Humanos , Masculino , Peru , Reação em Cadeia da Polimerase/instrumentação , Reação em Cadeia da Polimerase/métodosRESUMO
The establishment of baseline data on parasites from wild primates is essential to understand how changes in habitat or climatic disturbances will impact parasite-host relationships. In nature, multiparasitic infections of primates usually fluctuate temporally and seasonally, implying that the acquisition of reliable data must occur over time. Individual parasite infection data from two wild populations of New World primates, the saddleback (Leontocebus weddelli) and emperor (Saguinus imperator) tamarin, were collected over 3 years to establish baseline levels of helminth prevalence and parasite species richness (PSR). Secondarily, we explored variation in parasite prevalence across age and sex classes, test nonrandom associations of parasite co-occurrence, and assess the relationship between group size and PSR. From 288 fecal samples across 105 individuals (71 saddleback and 34 emperor tamarins), 10 parasite taxa were identified by light microscopy following centrifugation and ethyl-acetate sedimentation. Of these taxa, none were host-specific, Dicrocoeliidae and Cestoda prevalences differed between host species, Prosthenorchis and Strongylida were the most prevalent. Host age was positively associated with Prosthenorchis ova and filariform larva, but negatively with cestode and the Rhabditoidea ova. We detected no differences between expected and observed levels of co-infection, nor between group size and parasite species richness over 30 group-years. Logistic models of individual infection status did not identify a sex bias; however, age and species predicted the presence of four and three parasite taxa, respectively, with saddleback tamarins exhibiting higher PSR. Now that we have reliable baseline data for future monitoring of these populations, next steps involve the molecular characterization of these parasites, and exploration of linkages with health parameters.
Assuntos
Biodiversidade , Callitrichinae , Helmintíase Animal/epidemiologia , Helmintos/isolamento & purificação , Doenças dos Macacos/epidemiologia , Saguinus , Animais , Feminino , Helmintíase Animal/parasitologia , Masculino , Doenças dos Macacos/parasitologia , Peru/epidemiologia , PrevalênciaRESUMO
There is an increased interest in potential zoonotic malarias. To date, Plasmodium malariae that infects humans remains indistinguishable from Plasmodium brasilianum, which is widespread among New World primates. Distributed throughout tropical Central and South America, the Callitrichidae are small arboreal primates in which detection of natural Plasmodium infection has been extremely rare. Most prior screening efforts have been limited to small samples, the use of low-probability detection methods, or both. Rarely have screening efforts implemented a longitudinal sampling design. Through an annual mark-recapture program of two sympatric callitrichids, the emperor (Saguinus imperator) and saddleback (Saguinus fuscicollis) tamarins, whole blood samples were screened for Plasmodium by microscopy and nested PCR of the cytochrome b gene across four consecutive years (2012-2015). Following the first field season, approximately 50% of the samples collected each subsequent year were from recaptured individuals. In particular, out of 245 samples from 129 individuals, 11 samples from 6 individuals were positive for Plasmodium, and all but one of these infections was found in S. imperator. Importantly, the cytochrome b sequences were 100% identical to former isolates of P. malariae from humans and P. brasilianum from Saimiri sp. Chronic infections were detected as evidenced by repeated infections (7) from two individuals across the 4-year study period. Furthermore, 4 of the 5 infected emperor tamarins were part of a single group spanning the entire study period. Overall, the low prevalence reported here is consistent with previous findings. This study identifies two new natural hosts for P. brasilianum and provides evidence in support of chronic infections in wildlife populations. Given that callitrichids are often found in mixed-species associations with other primates and can be resilient to human-disturbed environments, they could contribute to the maintenance of P. malariae populations if future work provides entomological and epidemiological evidence indicating human zoonotic infections.