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1.
Rev. bras. ciênc. avic ; 21(1): [eRBCA-2019-0637], abr. 2019. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1490619

RESUMO

The present work was carried out to study the effect of in-ovo injection of ochratoxin A (OTA) as an oxidative stress and its consequences on hepatic and kidney functions, thyroid activity, and histological examination of brain and liver in chicken embryos and subsequently in the hatching chicks. On the 10th day of incubation, one hundred and sixty-two fertile eggs were randomly divided into two equal treatments. Control treatment, (injected by 50 µl sodium carbonate) and OTA treatment (injected by 12.5 ng OTA dissolved in 50 µl sodium carbonate). OTA treatement group significantly reduced glutathione (GSH) and significantly increased thiobarbituric acid reactive substances production (TBARS) in embryonic and hatched chicks regarding livers, spleen, bursa of Fabricius, heart, and brain as an indicator of oxidative stress. OTA injection increased TBARS and decreased GSH levels in both allantoic and amniotic fluids. On the 14th and 16th days of incubation and at the hatch, a significant lower concentration in cholesterol and higher concentrations of alanine amino transferase, aspartate amino transferase, alkaline phosphatase, gamma glutamyl transferase, acid phosphatase enzymes activities and triglycerides in the hepatic tissues of the OTA group were observed. Histological examination of OTA group of brain and liver tissues showed some degenerative changes through the experimental period. In conclusion, in-ovo OTA treated had teratogenic and embryotoxic effects.


Assuntos
Animais , Gravidez , Embrião de Galinha/citologia , Embrião de Galinha/química , Estresse Oxidativo , Ocratoxinas/análise
2.
R. bras. Ci. avíc. ; 21(1): [eRBCA-2019-0637], mai. 2019. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-21179

RESUMO

The present work was carried out to study the effect of in-ovo injection of ochratoxin A (OTA) as an oxidative stress and its consequences on hepatic and kidney functions, thyroid activity, and histological examination of brain and liver in chicken embryos and subsequently in the hatching chicks. On the 10th day of incubation, one hundred and sixty-two fertile eggs were randomly divided into two equal treatments. Control treatment, (injected by 50 µl sodium carbonate) and OTA treatment (injected by 12.5 ng OTA dissolved in 50 µl sodium carbonate). OTA treatement group significantly reduced glutathione (GSH) and significantly increased thiobarbituric acid reactive substances production (TBARS) in embryonic and hatched chicks regarding livers, spleen, bursa of Fabricius, heart, and brain as an indicator of oxidative stress. OTA injection increased TBARS and decreased GSH levels in both allantoic and amniotic fluids. On the 14th and 16th days of incubation and at the hatch, a significant lower concentration in cholesterol and higher concentrations of alanine amino transferase, aspartate amino transferase, alkaline phosphatase, gamma glutamyl transferase, acid phosphatase enzymes activities and triglycerides in the hepatic tissues of the OTA group were observed. Histological examination of OTA group of brain and liver tissues showed some degenerative changes through the experimental period. In conclusion, in-ovo OTA treated had teratogenic and embryotoxic effects.(AU)


Assuntos
Animais , Gravidez , Embrião de Galinha/química , Embrião de Galinha/citologia , Estresse Oxidativo , Ocratoxinas/análise
3.
Rev. bras. ciênc. avic ; 19(3): 371-376, July-Sept. 2017. tab
Artigo em Inglês | VETINDEX | ID: biblio-1490440

RESUMO

ABSTRACT The present study was carried out to study the effect of in-ovo ochratoxin A (OTA) injection in Highline layer eggs on calcium metabolism and blood biochemical parameters of embryos and after the hatch. At day 10 of embryonic development, one hundred and sixty-two fertile eggs were individually weighed and divided into two equal treatments. The first treatment (control) consisted of the individual injection of fertile eggs with 50 µL sodium carbonate. In the second treatment (OTA), fertile eggs were individually injected with 12.5 ng OTA dissolved in 50 µL sodium carbonate. On days 12, 14, and 16 of incubation and at the hatch, serum calcium and inorganic phosphorus concentrations were lower (p 0.05), while sodium, alkaline phosphatase and triiodothyronine concentrations were higher (p 0.05) in the OTA-injected eggs compared with the controls. Serum potassium concentration was not affected (p 0.05) by OTA treatment. Lower calcium and phosphorus levels were determined (p 0.05) in the allantoic fluid of OTA-injected eggs compared with the controls. On days 12, 14, and 16 of incubation and at the hatch, lower whole body and yolk calcium and phosphorus, but not sodium levels, were measured (p 0.05) in the OTA treatment compared with the controls. In conclusion, the injection of eggs with OTA reduced blood calcium and phosphorus levels, which were associated with reduced whole body and yolk content from these electrolytes. Therefore, ochratoxin A had a negative effect on calcium metabolism.


Assuntos
Animais , Aves Domésticas/fisiologia , Aves Domésticas/genética , Aves Domésticas/sangue , Eletrólitos/sangue , Teratogênicos , Fenômenos Bioquímicos
4.
R. bras. Ci. avíc. ; 19(3): 371-376, July-Sept. 2017. tab
Artigo em Inglês | VETINDEX | ID: vti-13868

RESUMO

ABSTRACT The present study was carried out to study the effect of in-ovo ochratoxin A (OTA) injection in Highline layer eggs on calcium metabolism and blood biochemical parameters of embryos and after the hatch. At day 10 of embryonic development, one hundred and sixty-two fertile eggs were individually weighed and divided into two equal treatments. The first treatment (control) consisted of the individual injection of fertile eggs with 50 µL sodium carbonate. In the second treatment (OTA), fertile eggs were individually injected with 12.5 ng OTA dissolved in 50 µL sodium carbonate. On days 12, 14, and 16 of incubation and at the hatch, serum calcium and inorganic phosphorus concentrations were lower (p 0.05), while sodium, alkaline phosphatase and triiodothyronine concentrations were higher (p 0.05) in the OTA-injected eggs compared with the controls. Serum potassium concentration was not affected (p 0.05) by OTA treatment. Lower calcium and phosphorus levels were determined (p 0.05) in the allantoic fluid of OTA-injected eggs compared with the controls. On days 12, 14, and 16 of incubation and at the hatch, lower whole body and yolk calcium and phosphorus, but not sodium levels, were measured (p 0.05) in the OTA treatment compared with the controls. In conclusion, the injection of eggs with OTA reduced blood calcium and phosphorus levels, which were associated with reduced whole body and yolk content from these electrolytes. Therefore, ochratoxin A had a negative effect on calcium metabolism.(AU)


Assuntos
Animais , Aves Domésticas/genética , Aves Domésticas/fisiologia , Aves Domésticas/sangue , Eletrólitos/sangue , Teratogênicos , Fenômenos Bioquímicos
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