RESUMO
OBJECTIVE: This comparative study was designed to analyze the potential effects of Yaji (suya meat sauce) and its composite spices on male fertility based on testicular histology, serum testosterone level, and semen analysis parameters. METHODS: The study included 70 adult male Sprague Dawley rats with an average weight of 120 g. They were divided into two experimental study groups, respectively analyzed for 28 and 56 days. Each group featured 35 rats, further subdivided into seven treatment groups (A - G; n=5 each). Group A - Control; Group B: 200 mg/kg of Yaji; Group C: 200 mg/kg of red pepper; Group D: 200 mg/kg of black pepper; Group E: 200 mg/kg of clove; Group F: 200 mg/kg of ginger; and Group G: 200 mg/kg of garlic given orally using an oral cannula. At the end of the experiment, the animals were euthanized. Blood samples collected via cardiac puncture and their testes were excised and weighed. The cauda epididymis was excised for semen analysis using a Neubauer Counting Chamber (hemocytometer) and the testes were fixed in Bouin solution, processed, and stained with Hematoxylin and Eosin. RESULTS: Significant increases (p<0.05) were seen in body weight, testicular weight, serum testosterone level, sperm count and motility in the Yaji treated groups, in addition to significant increases in serum testosterone level, sperm counts, and sperm motility, and enhanced spermatogenesis and proliferation of Leydig cells in vivo as compared to the groups given isolated component spices (groups C-G), which also showed significant changes in testosterone and semen analysis when compared with the control groups. CONCLUSIONS: Yaji or its spice components can boost male fertility parameters when consumed in moderated quantities without the known cytotoxic additives or condiments such as monosodium glutamate.
Assuntos
Motilidade dos Espermatozoides , Especiarias , Animais , Masculino , Carne , Ratos , Ratos Sprague-Dawley , Contagem de Espermatozoides , EspermatozoidesRESUMO
Ubiquitous consumption of Momordica charantia is hinged on credence of its potency in alleviating ailments. Almost no data exist on its quantitative relationship to the testes. The aim was to study the histomorphometric effect of Momordica charantia seed extract (MC), ascorbic acid (C), alpha-tocopherol (E) and testosterone (T) on the testes of rats. Ninety five mature 6-8 week-old male Sprague-Dawley (S-D) rats allotted randomly into six groups. Group I: fed MC for 16 weeks. Group II: administered MC for 8 weeks, later distilled water for another 8 weeks. Group III: administered C, E, T and a combination of all three test solutions (CET) for 8 weeks and later fed MC for another 8 weeks. Group IV: received MC initially for 8 weeks and later administered C, E, T and CET for another 8 weeks. Group V: fed concurrently the extract, C, E, T and CET for 8 weeks. Group VI (control): administered C, E, T, CET and distilled water for 16 weeks. The doses for MC, C, E and T were 50 mg/100 g b.w/oral/day, 0.01, 20.0 and 0.05 mg/kg. The animals were sacrificed; testes estimated for volume. Diameter, cross sectional area (AC), number of profiles per unit area (NA), length density (LV) and numerical density (NV) of the seminiferous tubules determined. The mean testicular volumes, tubular diameters and AC showed significant decline (p 0.05) in Groups I and III compared to controls. On cessation of MC values recovered to baseline control value. Groups IV and V, were not significantly (p 0.05) different from controls. There was a significant (p 0.05) increase in NA, LV and NV of seminiferous tubules in Groups I and III compared to controls. Histomorphometric data supports a reversible deleterious effect of MC on testes.
El consumo ubicuo de Momordica charantia ha girado en torno a su potencial participación en el alivio de dolencias. El objetivo fue estudiar los efectos del extracto de la semilla de Momordica charantia (MC), ácido ascórbico (C), alfa-tocoferol (E) y testosterona (T) sobre parámetros histomorfométricos en testículos de ratas. Noventa y cinco ratas machos Sprague-Dawley (S-D) maduras (6-8 semanas de edad) fueron asignadas al azar en seis grupos. Grupo I: alimentados con MC durante 16 semanas. Grupo II: MC administrado durante 8 semanas, luego agua destilada por otras 8 semanas. Grupo III: C, E, T administrados junto a una combinación de las tres soluciones de prueba (CET) durante 8 semanas y luego alimentados con MC por otras 8 semanas. Grupo IV: MC inicialmente durante 8 semanas y posteriormente C, E, T y CET por otras 8 semanas. Grupo V: alimentado al mismo tiempo con el extracto, C, E, T y CET durante 8 semanas. Grupo VI (control): administrado con C, E, T, CET y agua destilada durante 16 semanas. Las dosis de MC, C, E y T fueron de 50 mg/100 g de peso corporal/oral/día, 0,01, 20,0 y 0,05 mg/kg. Los animales fueron sacrificados y se estimó el volumen de los testículos. El diámetro, área de sección transversal (AC), el número de perfiles por unidad de área (NA), la densidad de longitud (LV) y la densidad numérica (NV) de los túbulos seminíferos fueron determinados. Los volúmenes medio testicular, diámetro de túbulos y AC mostraron una disminución significativa (p 0,05) en los grupos I y III en comparación con los controles. Al quitar MC, los valores se recuperaron al valor basal de control. Los grupos IV y V, no fueron significativamente diferentes de los controles (p 0,05). Hubo una diferencia significativa (p 0,05) con aumento de la NA, LV y NV de los túbulos seminíferos de los grupos I y III en comparación con los controles. Los datos histomorfométricos apoyan la reversibilidad de los efectos nocivos de la MC en los testículos.