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The aim of this study was to analyze the immunogenic response elicited in swine by two synthetic peptides derived from GP5 to understand the role of lineal B epitopes in the humoral and B-cell-mediated response against the porcine reproductive and respiratory syndrome virus (PRRSV). For inoculation, twenty-one-day-old pigs were allocated into six groups: control, vehicle, vaccinated (Ingelvac-PRRSV, MLV®), non-vaccinated and naturally infected, GP5-B and GP5-B3. At 2 days post-immunization (dpi), the GP5-B3 peptide increased the serum concentrations of cytokines associated with activate adaptive cellular immunity, IL-1ß (1.15 ± 1.15 to 10.17 ± 0.94 pg/mL) and IL-12 (323.8 ± 23.3 to 778.5 ± 58.11 pg/mL), compared to the control group. The concentration of IgGs anti-GP5-B increased in both cases at 21 and 42 dpi compared to that at 0 days (128.3 ± 8.34 ng/mL to 231.9 ± 17.82 and 331 ± 14.86 ng/mL), while IgGs anti-GP5-B3 increased at 21 dpi (105.1 ± 19.06 to 178 ± 15.09 ng/mL) and remained at the same level until 42 dpi. Also, antibody-forming/Plasma B cells (CD2+/CD21-) increased in both cases (9.85 ± 0.7% to 13.67 ± 0.44 for GP5-B and 15.72 ± 1.27% for GP5-B3). Furthermore, primed B cells (CD2-/CD21+) from immunized pigs showed an increase in both cases (9.62 ± 1.5% to 24.51 ± 1.3 for GP5-B and 34 ± 2.39% for GP5-B3) at 42 dpi. Conversely the naïve B cells from immunized pigs decreased compared with the control group (8.84 ± 0.63% to 6.25 ± 0.66 for GP5-B and 5.78 ± 0.48% for GP5-B3). Importantly, both GP5-B and GP5-B3 peptides exhibited immunoreactivity against serum antibodies from the vaccinated group, as well as the non-vaccinated and naturally infected group. In conclusion, GP5-B and GP5-B3 peptides elicited immunogenicity mediated by antigen-specific IgGs and B cell activation.
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This study aimed to investigate the immunomodulatory behavior of soluble immune checkpoints (sICPs) and other biomarkers in the pathophysiology of SARS-CoV-2 infection. The study included 59 adult participants, 43 of whom tested positive for SARS-CoV-2. Patients were divided into three cohorts: those with moderate disease (n = 16), recovered patients with severe disease (n = 13), and deceased patients with severe disease (n = 16). In addition, 16 participants were pre-pandemic subjects negative for SARS-CoV-2. The relative activity of neutralizing antibodies (rNAbs) against SARS-CoV-2 and the values of 14 sICPs in peripheral blood were compared between the four groups. Because the increase of markers values of inflammation [NLR > 12; CRP > 150 mg/L] and venous thromboembolism [D-dimer > 0.5 mg/L] has been associated with mortality from COVID-19, the total and differential leukocyte counts, the NLR, and CRP and D-dimer values were obtained in patients with severe disease. No differences in rNAbs were observed between the cohorts. Only the levels of five sICPs, sCD27, sHVEM sTIM-3, sPD-1, and sPDL-1, were significantly higher in patients with severe rather than moderate disease. The sPDL-2 level and NLR were higher in deceased patients than in recovered patients. However, there was no difference in CRP and D-dimer values between the two groups. Of the five soluble biomarkers compared among patients with severe disease, only sPDL-2 was higher in deceased patients than in recovered patients. This suggests that immuno-inhibitory sICPs might be used as indicators for severe COVID-19, with sPDL-2 used to assess individual risk for fatality.IMPORTANCECOVID-19, the disease caused by a SARS-CoV-2 infection, generates a broad spectrum of clinical symptoms, progressing to multiorgan failure in the most severe cases. As activation of the immune system is pivotal to eradicating the virus, future research should focus on identifying reliable biomarkers to efficiently predict the outcome in severe COVID-19 cases. Soluble immune checkpoints represent the function of the immune system and are easily determined in peripheral blood. This research could lead to implementing more effective severity biomarkers for COVID-19, which could increase patients' survival rate and quality of life.
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Biomarcadores , COVID-19 , SARS-CoV-2 , Humanos , COVID-19/imunologia , COVID-19/mortalidade , COVID-19/sangue , Masculino , Feminino , Pessoa de Meia-Idade , Biomarcadores/sangue , SARS-CoV-2/imunologia , Idoso , Adulto , Índice de Gravidade de Doença , Anticorpos Neutralizantes/sangue , Anticorpos Neutralizantes/imunologia , Proteínas de Checkpoint Imunológico/sangue , Produtos de Degradação da Fibrina e do Fibrinogênio/análise , Idoso de 80 Anos ou maisRESUMO
We analyzed the T-cell responses induced by lineal epitopes of glycoprotein 5 (GP5) from PRRSV to explore the role of this protein in the immunological protection mediated by T-cells. The GP5 peptides were conjugated with a carrier protein for primary immunization and booster doses. Twenty-one-day-old pigs were allocated into four groups (seven pigs per group): control (PBS), vehicle (carrier), PTC1, and PTC2. Cytokine levels were measured at 2 days post-immunization (DPI) from serum samples. Cytotoxic T-lymphocytes (CTLs, CD8+) from peripheral blood were quantified via flow cytometry at 42 DPI. The cytotoxicity was evaluated by co-culturing primed lymphocytes with PRRSV derived from an infectious clone. The PTC2 peptide increased the serum concentrations of pro-inflammatory cytokines (i.e., TNF-α, IL-1ß, IL-8) and cytokines that activate the adaptive cellular immunity associated with T-lymphocytes (i.e., IL-4, IL-6, IL-10, and IL-12). The concentration of CTLs (CD8+) was significantly higher in groups immunized with the peptides, which suggests a proliferative response in this cell population. Primed CTLs from immunized pigs showed cytolytic activity in PRRSV-infected cells in vitro. PTC1 and PTC2 peptides induced a protective T-cell-mediated response in pigs immunized against PRRSV, due to the presence of T epitopes in their sequences.
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Síndrome Respiratória e Reprodutiva Suína , Vírus da Síndrome Respiratória e Reprodutiva Suína , Vacinas Virais , Suínos , Animais , Vírus da Síndrome Respiratória e Reprodutiva Suína/metabolismo , Síndrome Respiratória e Reprodutiva Suína/prevenção & controle , Anticorpos Antivirais , Citocinas/metabolismo , Fator de Necrose Tumoral alfa , EpitoposRESUMO
Resumen Objetivo. Reconstruir las teorías subjetivas de profesionales en trabajo social y psicología sobre la disciplina parental. Método. Es una investigación cualitativa con diseño descriptivo. Se realizaron entrevistas individuales a siete profesionales en trabajo social y siete en psicología de la cuarta región de Chile. El análisis de datos utilizó codificaciones mediante el software ATLAS.ti. Resultados. Se demostró que los consejos de disciplina parental se conforman por la formación profesional de pre y posgrado, la experiencia laboral en programas con familias y elementos personales relacionados con historia de vida y parentalidad. Es así que los consejos se basan principalmente en teorías subjetivas y profesionales que se relacionan con teorías formales. Se observan diferencias en los consejos que entregan ambas profesiones.
Abstract Objective. To reconstruct the subjective theories of social workers and psychologists regarding parental discipline. Method. The following research is qualitative, and it was carried out through a descriptive type of design. Individual interviews were conducted to seven social workers and seven psychologists from the fourth region of Chile. The analysis of the collected data was performed through the grounded theory using encodings from the ATLAS.ti. software. Results. It is shown that the pieces of advice regarding parental discipline are strongly influenced by the professional training, the working experience, and some personal elements and conceptions of the interviewed professionals. Some differences are observed regarding the pieces of advice provided from both professional perspectives.
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Humanos , Masculino , Feminino , Adulto , Psicologia , Serviço Social , Relações Familiares/psicologia , Satisfação Pessoal , ChileRESUMO
BACKGROUND: Our purpose was to provide a detailed clinical description, of symptoms and laboratory abnormalities, and temporality in patients with confirmed Zika and dengue infections, and other acute illnesses of unidentified origin (AIUO). METHODS/ PRINCIPAL FINDINGS: This was a two-year, multicenter, observational, prospective, cohort study. We collected data from patients meeting the Pan American Health Organization's modified case-definition criteria for probable Zika infection. We identified Zika, dengue chikungunya by RT-PCR in serum and urine. We compared characteristics between patients with confirmed Zika and dengue infections, Zika and AIUO, and Dengue and AIUO at baseline, Days 3,7,28 and 180 of follow-up. Most episodes (67%) consistent with the PAHO definition of probable Zika could not be confirmed as due to any flavivirus and classified as Acute Illnesses of Unidentified Origin (AIUO). Infections by Zika and dengue accounted for 8.4% and 16% of episodes. Dengue patients presented with fever, generalized non-macular rash, arthralgia, and petechiae more frequently than patients with Zika during the first 10 days of symptoms. Dengue patients presented with more laboratory abnormalities (lower neutrophils, lymphocytosis, thrombocytopenia and abnormal liver function tests), with thrombocytopenia lasting for 28 days. Zika patients had conjunctivitis, photophobia and localized macular rash more frequently than others. Few differences persisted longer than 10 days after symptoms initiation: conjunctivitis in Zika infections, and self-reported rash and petechia in dengue infections. CONCLUSIONS: Our study helps characterize the variety and duration of clinical features in patients with Zika, dengue and AIUO. The lack of diagnosis in most patients points to need for better diagnostics to assist clinicians in making specific etiologic diagnoses.
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Dengue/diagnóstico , Febre de Causa Desconhecida/diagnóstico , Infecção por Zika virus/diagnóstico , Adolescente , Adulto , Idoso , Febre de Chikungunya/diagnóstico , Febre de Chikungunya/epidemiologia , Vírus Chikungunya/isolamento & purificação , Criança , Estudos de Coortes , Dengue/epidemiologia , Vírus da Dengue/isolamento & purificação , Feminino , Febre de Causa Desconhecida/epidemiologia , Humanos , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Estudos Prospectivos , Zika virus/isolamento & purificação , Infecção por Zika virus/epidemiologiaRESUMO
La osteonecrosis de maxilar asociada a aminobisfosfonatos (BRONJ) constituye un efecto secundario del tratamiento crónico con los más potentes. Un modelo experimental permitiría determinar la patogenia de dicha alteración. La oveja presenta características orales y del metabolismo óseo similar al humano y permite realizar manipulaciones bucales. Se evaluaron cambios clínicos, remodelación ósea y masa ósea maxilar en ovejas hembras adultas tratadas con zolendronato (ZOL), durante 22 meses y utilizando dosis equivalente al tratamiento de neoplasias. Seis ovariectomizadas (OVX) recibieron ZOL; 5 OVX y 4 SHAM (control) recibieron solución fisiológica. Al inicio, 4 y 22 meses se evaluó calcemia, fosfatemia, crosslaps (CTX) y fosfatasa alcalina ósea. Al final, se evaluó contenido mineral óseo de la hemimandíbula superior (CMO: mg/cm2). Al final del estudio, CTX disminuyó significativamente en ZOL (p<0,05) sin diferencias entre SHAM y OVX. En maxilar, los contenidos de Ca y P (g/g tejido) y CMO (g/cm2 ) disminuyeron en OVX vs. SHAM (p<0,05) y solo Ca y CMO respecto de ZOL (p<0,05). ZOL incrementó el contenido de Ca y CMO, mientras que el de P permaneció significativamente disminuido respecto de SHAM. La sobrevida en SHAM y OVX fue del 100% y en ZOL 77% (2 muertes); 2 ovejas del grupo ZOL presentaron necrosis de maxilar. Conclusiones: fue posible obtener desarrollo de BRONJ por tratamiento crónico con ZOL, el cual redujo notablemente la resorción y, según la relación Ca/P, posiblemente haya afectado la mineralización ósea. (AU)
Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a complication of chronic treatment with the most powerful aminobisphosphonates (BPs). An experimental animal model would allow to determine the pathogenesis of this complication. Ewes exhibit similar oral cavity characteristics and bone metabolism as humans, and they are suitable for oral cavity interventions. We examined herein the clinical manifestations, bone remodeling status, and maxillary bone mass in adult female ewes treated with zoledronate (ZOL) for 22 months. Six ovariectomized (OVX) ewes received ZOL; and 5 OVX and 4 SHAM animals received saline solution. At the start of the experiment, and at the 4 and 22 month-time points serum Ca, P, crosslaps (CTX), and bone alkaline phosphatase were measured. Bone mineral content (BMC) of the superior hemimandible was measured at the end of the experiment. At this time point, CTX was significantly decreased only in the ZOL group (p<0.05). Ca and P content (g/g tissue) and BMC in the mandible were significantly decreased in the OVX group compared to SHAM animals (p<0.05) and only Ca content and BMC were decreased when compared to ZOL (p<0.05). ZOL treatment increased the Ca content and BMC, whereas the P content remained low compared to the SHAM group (p<0.05). All ewes from the SHAM and OVX groups and 77% of the animals from the ZOL group survived until the end of the experiment, whereas two ewes of ZOL group exhibited BRONJ. Conclusion: under our experimental conditions, it was possible to induce BRONJ by the chronic ZOL administration, which in turn induced a high reduction in bone resorption as well as possibly impaired bone mineralization, based on the Ca/P ratio in the mandible. (AU)
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Animais , Difosfonatos/efeitos adversos , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/patologia , Ácido Zoledrônico/efeitos adversos , Extração Dentária , Doenças Ósseas Metabólicas/induzido quimicamente , Ovinos/metabolismo , Ovinos/sangue , Biomarcadores/sangue , Densidade Óssea/efeitos dos fármacos , Remodelação Óssea/efeitos dos fármacos , Densitometria , Desenvolvimento Experimental , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/etiologia , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/imunologia , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/prevenção & controle , Ácido Zoledrônico/administração & dosagem , Glucocorticoides/uso terapêutico , Analgésicos/uso terapêutico , Ílio/citologia , Anestésicos Dissociativos/uso terapêutico , Lidocaína/uso terapêutico , Maxila/citologia , Maxila/efeitos dos fármacos , Maxila/metabolismo , Maxila/diagnóstico por imagem , Antibacterianos/uso terapêuticoRESUMO
RESUMEN La crisis económica iniciada en 2008 ha conllevado que el saldo migratorio español, tras más de dos décadas siendo positivo, volviera a partir de 2011 a ser negativo, como lo fue durante gran parte del siglo XX. Pero, a diferencia de los emigrados de antaño, los actuales son profesionistas entre 25 y 35 años y sin cargas familiares. El objetivo del presente artículo fue establecer cómo los jóvenes españoles reconstruyen sus identidades a lo largo de sus procesos migratorios y qué factores influyen en ello. Se realizaron una serie de entrevistas en profundidad, encaminadas a obtener los relatos de vida de 15 jóvenes con experiencias migratorias en el periodo de 2008 a 2014. El análisis de la información con seguida, siguiendo la Grounded Theory, posibilita la determinación de tres categorías dis cursivas en la evolución de las identidades de esta población migrante, las cuales se tipifican aquí como la españofobia (rechazo de la sociedad española), la españofilia (añoranza de la sociedad española) y la mutación a una nueva identidad híbrida, en la que los patrones culturales de origen se entremezclan con los de la nueva sociedad donde residen. Las sucesivas transformaciones en las identidades de los jóvenes migrantes, que se tratan de conceptuar a través de dichas categorías, están influenciadas por una serie de factores, tales como su nivel de conocimiento del idioma del país receptor, su grado de habituación al clima de dicho país, el estatus social logrado tras la emigración y las interacciones con la población autóctona, entre otras. Por lo tanto, dichas identidades no son esenciales o estáticas, sino construcciones sociales que se van definiendo y redefiniendo (construyendo y reconstruyendo), según las situaciones de interacción (sociocultural y/o psicológica) que el proceso migratorio propicia.
ABSTRACT The economic crisis begun in 2008 caused that the migratory balance of Spain, after more than two decades of being positive, in 2011 became again negative, as it was for much of the twentieth century. However, unlike the emigrants of yesteryear, today's university emigrants are between 25 and 35 years old and without family dependents. This article examines how these new Spanish emigrants rebuild their identities during their migration processes and the factors that influence them. For this purpose, in-depth interviews were carried out with the aim of knowing the life stories of 15 young people with migration experiences during the period from 2008 to 2014. The information obtained is analyzed following the Grounded Theory method, which enables the differentiation of three discursive categories in the evolution of the identities of the young university emigrants. So, these identities are named here as Spanish-phobia (Rejection of Spanish society), Spanish-philia (longing for the Spanish society), and the mutation to a new hybrid identity, in which cultural patterns of origin are intermingled with those of the new society where the migrants live. By means of the said three categories the successive transformations in the identities of young migrants are conceptualized. In turn, these transformations depend upon a series of factors, such as the degree of knowledge that migrants have about the host country language, their degree of habituation to the climate of that country, the social status they achieve after migration, their interactions with the local population, among others. Therefore, the above mentioned three identities are not essential or static, but social constructions that are defined and/or redefined (built and/or rebuilt) according to the situations of interaction (socio-cultural and/or psychological) entailed by the migratory process.
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This case study presents the fate of selected organic, priority and emerging pollutants along a 3.6km sector of a vegetated, agricultural ditch situated in Sinaloa (Mexico). The ditch receives runoff of agriculture and domestic wastewater from an adjacent community. During 2013, the occurrence of 38 organic pollutants (pesticides, polycyclic aromatic hydrocarbons (PAHs), artificial sweeteners and pharmaceutical residues) was monitored monthly at five selected points in the ditch water. Additionally, sediment and Typha domingensis (cattail) plants were collected in March, June, and September 2013 and investigated concerning their ability to absorb and accumulate pollutants. The concentrations of the selected pollutants in the ditch water ranged from sub ngL-1 (metolachlor, atrazine) to µgL-1 (metalaxyl, acesulfame). The metabolites endosulfan sulfate and endosulfan lactone exceeded mostly the concentration of the precursor insecticide endosulfan. Sorption on sediments was of minor relevance for accumulation of pollutants in the ditch system. Concentrations in the sediments varied seasonally and ranged from 0.2 to 12,432µgkg-1 dry weight (d.w.). T. domingensis accumulated ten of the studied pollutants mainly in roots (5-1065µgkg-1 d.w.). Overall, the monitoring results of the ditch compartments indicated that downstream the concentrations of the target pollutants decreased. Under no-flow conditions in the hot season, the ditch revealed a noticeable potential to mitigate pollutants. Among the high microbial activity in the water and the subtropical climate conditions, the ditch vegetation contributed to natural attenuation of the selected pollutants.
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Las lesiones penetrantes por arma blanca a nivel espinal son relativamente raras. Estas lesiones podrían ser fácilmente diagnosticadas al examen inicial o podrían tener complicaciones tardías que son incapacitantes y potencialmente letales. Algunas de estas lesiones requieren tratamiento neuroquirúrgico. Se presenta un cso en el cual un paciente con lesión penetrante por arma blanca se complicó tardíamente con una fístula de líquido cefalorraquídeo y meningitis bacteriana debido a cuerpo extraño retenido no diagnosticado inicialmente, fragmento de cuchillo.
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Humanos , Masculino , Adulto , Líquido Cefalorraquidiano , Meningite , Infecção dos Ferimentos , Ferimentos e Lesões , Ferimentos Penetrantes , Costa RicaRESUMO
Profound changes are under way in Cuban medical education. Some aspects of this transformation represent radical shifts, others a deepening of processes already in motion. Together, these changes reflect a progressive sense of urgency over the last four decades to: 1) scale up physician training to meet the needs of the whole population; 2) recruit and train scientifically prepared and socially committed students; and 3) match competencies, knowledge base, and scope of responsibilities to the concrete health needs of people in Cuba and other countries where these future physicians may serve. These three goals have guided successive innovations in Cuban medical education since the early 1960s, when the University of Havana Medical School was left with only 23 of its 161 professors - the rest either emigrating or in disagreement with academic and health care reforms designed to guarantee the right to health care. From a fee-for-service model catering mainly to individual patients, health care was being transformed into a universal public health system. This required decentralization of medical services - first to the rural areas of the country, which had essentially gone without - followed by development of a nationwide primary health care network. Thus, from the beginning, there was an urgent need to train many more physicians and to train physicians prepared for, and committed to, this new vision.
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Se hace una revisión de los estudios de cistografía nuclear practicados en el servicio en un período de 23 meses considerando tanto la parte técnica como los resultados, para determinar indicaciones, ventajas y desventajas de dicho método en el diagnóstico y seguimiento de pacientes con reflujo vesicoureteral