RESUMO
The potential use of anodised zirconium as permanent implant has been investigated. Zirconium was anodised at constant potential between 3 and 30 V in H(3)PO(4). Electrochemical assays were conducted in simulated body fluid solution (SBF) in order to evaluate the effect of the surface oxide on the corrosion resistance in vitro after 30 days of immersion. The rupture potential increases when increasing thickness of the anodic surface film. The increase in the barrier effect when increasing anodising potential is also verified by EIS. Anodisation in H(3)PO(4) proved to increase the apatite formation capability of zirconium in a single step. In vivo bone formation was also analysed by implanting the modified materials in Wistar rats. Anodised Zr presents higher corrosion resistance in SBF in all the studied immersion times when compared with non anodised Zr. Additionally, in vivo experiments evidence bone generation and growth in contact with zirconium implants both in the as-received and anodised condition.
Assuntos
Eletrodos , Próteses e Implantes , Zircônio/química , Animais , Técnicas Eletroquímicas , Técnicas In Vitro , Ratos , Ratos Wistar , Propriedades de SuperfícieRESUMO
The aim of this study was to evaluate histologically the biological effect of pitting corrosion and to contribute clinically relevant data on the permanence of titanium metal structures used in osteosynthesis in the body. Commercially pure titanium laminar implants (control) and commercially pure titanium laminar implants with pitting corrosion (experimental) were implanted in the tibiae of rats. At 14 days post-implantation the animals were killed. The tibiae were resected, fixed, radiographed and processed for embedding in methyl methacrylate. Percentage of bone-implant contact and peri-implant bone volume were evaluated. The histological study of the titanium implants submitted to pitting corrosion showed scarce bone-implant contact, it was only present in the areas with no pitting and/or surface alterations. There was a statistically significant lower percentage of bone-implant contact in the experimental group (6%+/-4) than in the control group (26%+/-6) (p<0.001). Products of corrosion in the peri-implant bed, especially around the blood vessels and areas of bone marrow in the metal-tissue interface, were observed. The microchemical analysis of corrosion products revealed the presence of titanium. The adverse local effects caused by pitting corrosion suggest that titanium plates and grids should be used with caution as permanent fixation structures.
Assuntos
Implantes Dentários/efeitos adversos , Materiais Dentários/efeitos adversos , Osseointegração/efeitos dos fármacos , Tíbia/ultraestrutura , Titânio/efeitos adversos , Animais , Regeneração Óssea/efeitos dos fármacos , Corrosão , Materiais Dentários/química , Masculino , Ratos , Ratos Wistar , Propriedades de Superfície , Tíbia/cirurgia , Titânio/químicaRESUMO
Corrosion phenomena would appear to play a decisive role in metallic implant long term behaviour. This study presents a method to correlate results obtained in "in vivo" and "in vitro" studies on materials used for metallic implants. Samples of titanium and copper immersed in saline solution (pH 7.4 and 5.2) were used for the "in vitro" study. Implants of these same materials were placed in rat tibiae following the method previously described by Cabrini et al. The animals were killed 14 days post-implantation, the tibiae were resected, radiographed and embedded in acrylic resin. Polarization curves revealed high corrosion in copper implants and low corrosion in titanium implants. It is important to point out that the titanium samples suffered slightly higher corrosion when immersed in a lower pH medium (pH 5.2), as in chronic inflammatory processes. A passive film was found on titanium samples as opposed to the strong corrosion observed in the copper implants. Microscopy revealed osseointegration around titanium implants and a severe inflammatory reaction with abscesses surrounding the copper implants. The method presented herein would allow to correlate "in vivo" and "in vitro" studies on corrosion in different implant materials and establish their relation with cell response.
Assuntos
Implantes Dentários , Materiais Dentários/química , Abscesso/patologia , Animais , Materiais Biocompatíveis/química , Cobre/química , Corrosão , Eletroquímica , Concentração de Íons de Hidrogênio , Teste de Materiais , Modelos Animais , Osseointegração , Ratos , Cloreto de Sódio/química , Tíbia/patologia , Tíbia/cirurgia , Titânio/químicaRESUMO
Corrosion phenomena would appear to play a decisive role in metallic implant long term behaviour. This study presents a method to correlate results obtained in [quot ]in vivo[quot ] and [quot ]in vitro[quot ] studies on materials used for metallic implants. Samples of titanium and copper immersed in saline solution (pH 7.4 and 5.2) were used for the [quot ]in vitro[quot ] study. Implants of these same materials were placed in rat tibiae following the method previously described by Cabrini et al. The animals were killed 14 days post-implantation, the tibiae were resected, radiographed and embedded in acrylic resin. Polarization curves revealed high corrosion in copper implants and low corrosion in titanium implants. It is important to point out that the titanium samples suffered slightly higher corrosion when immersed in a lower pH medium (pH 5.2), as in chronic inflammatory processes. A passive film was found on titanium samples as opposed to the strong corrosion observed in the copper implants. Microscopy revealed osseointegration around titanium implants and a severe inflammatory reaction with abscesses surrounding the copper implants. The method presented herein would allow to correlate [quot ]in vivo[quot ] and [quot ]in vitro[quot ] studies on corrosion in different implant materials and establish their relation with cell response.
RESUMO
Corrosion phenomena would appear to play a decisive role in metallic implant long term behaviour. This study presents a method to correlate results obtained in [quot ]in vivo[quot ] and [quot ]in vitro[quot ] studies on materials used for metallic implants. Samples of titanium and copper immersed in saline solution (pH 7.4 and 5.2) were used for the [quot ]in vitro[quot ] study. Implants of these same materials were placed in rat tibiae following the method previously described by Cabrini et al. The animals were killed 14 days post-implantation, the tibiae were resected, radiographed and embedded in acrylic resin. Polarization curves revealed high corrosion in copper implants and low corrosion in titanium implants. It is important to point out that the titanium samples suffered slightly higher corrosion when immersed in a lower pH medium (pH 5.2), as in chronic inflammatory processes. A passive film was found on titanium samples as opposed to the strong corrosion observed in the copper implants. Microscopy revealed osseointegration around titanium implants and a severe inflammatory reaction with abscesses surrounding the copper implants. The method presented herein would allow to correlate [quot ]in vivo[quot ] and [quot ]in vitro[quot ] studies on corrosion in different implant materials and establish their relation with cell response.