RESUMO
The estuary of Río de la Plata, in the eastern coast of South America, is a highly anthropized area that brings a high load of contaminants to the surrounding waters, which may have detrimental effects on the local marine fauna. The franciscana dolphin (Pontoporia blainvillei) is a small cetacean species endemic of the southwestern Atlantic Ocean listed as Vulnerable in the IUCN red list. In this study, we assessed the concentrations of 13 trace elements in bone samples from 100 franciscana dolphins that were found stranded dead or incidentally bycaught in the Río de la Plata and adjacent coast between 1953 and 2015. Elements were, in decreasing order of mean concentrations: Zn > Sr > Fe > Al > Mn > Cu > Pb > Cr > Ni > As > Hg > Cd > Se. The concentrations of Al, Cr and Fe were slightly higher in females than in males. The concentrations of As, Ni, and Pb significantly decreased with body length. Throughout the study period, the concentrations of Al, Cr, Cu, Fe, Mn and Ni significantly increased, while the concentrations of As, Pb and Sr significantly decreased. The increasing trends may be due to increased inputs from river discharges, the leather industry and petroleum refineries, while the decrease in Pb may be due to the ban in the use of this element as an additive in gasoline and as component of car batteries. This investigation supports the validity of analysing trace element in bone, a tissue available in scientific collections and museums, to retrospectively examine variation over long temporal scales and thus assess long-term trends in pollution.
Assuntos
Golfinhos , Oligoelementos , Poluentes Químicos da Água , Animais , Oceano Atlântico , Monitoramento Ambiental , Estuários , Feminino , Masculino , Estudos Retrospectivos , América do Sul , Oligoelementos/análise , Poluentes Químicos da Água/análiseRESUMO
Stable isotope analyses have become an important tool in reconstructing diets, analysing resource use patterns, elucidating trophic relations among predators and understanding the structure of food webs. Here, we use stable carbon and nitrogen isotope ratios in bone collagen to reconstruct and compare the isotopic niches of adult South American fur seals (Arctocephalus australis; n = 86) and sea lions (Otaria flavescens; n = 49) - two otariid species with marked morphological differences - in the Río de la Plata estuary (Argentina - Uruguay) and the adjacent Atlantic Ocean during the second half of the 20th century and the beginning of the 21st century. Samples from the middle Holocene (n = 7 fur seals and n = 5 sea lions) are also included in order to provide a reference point for characterizing resource partitioning before major anthropogenic modifications of the environment. We found that the South American fur seals and South American sea lions had distinct isotopic niches during the middle Holocene. Isotopic niche segregation was similar at the beginning of the second half of the 20th century, but has diminished over time. The progressive convergence of the isotopic niches of these two otariids during the second half of the 20th century and the beginning of the 21st century is most likely due to the increased reliance of South American fur seals on demersal prey. This recent dietary change in South American fur seals can be explained by at least two non-mutually exclusive mechanisms: (i) the decrease in the abundance of sympatric South American sea lions as a consequence of small colony size and high pup mortality resulting from commercial sealing; and (ii) the decrease in the average size of demersal fishes due to intense fishing of the larger class sizes, which may have increased their accessibility to those eared seals with a smaller mouth gape, that is, South American fur seals of both sexes and female South American sea lions.
Assuntos
Isótopos de Carbono/farmacocinética , Otárias , Isótopos de Nitrogênio/farmacocinética , Leões-Marinhos , Animais , Argentina , Oceano Atlântico , Osso e Ossos/química , Feminino , MasculinoRESUMO
Most otariids have colony-specific foraging areas during the breeding season, when they behave as central place foragers. However, they may disperse over broad areas after the breeding season and individuals from different colonies may share foraging grounds at that time. Here, stable isotope ratios in the skull bone of adult Galapagos sea lions (Zalophus wollebaeki) were used to assess the long-term fidelity of both sexes to foraging grounds across the different regions of the Galapagos archipelago. Results indicated that the stable isotope ratios (δ(13)C and δ(15)N) of sea lion bone significantly differed among regions of the archipelago, without any significant difference between sexes and with a non significant interaction between sex and region. Moreover, standard ellipses, estimated by Bayesian inference and used as a measure of the isotopic resource use area at the population level, overlapped widely for the sea lions from the southern and central regions, whereas the overlap of the ellipses for sea lions from the central and western regions was small and non-existing for those from the western and southern regions. These results suggest that males and females from the same region within the archipelago use similar foraging grounds and have similar diets. Furthermore, they indicate that the exchange of adults between regions is limited, thus revealing a certain degree of foraging philopatry at a regional scale within the archipelago. The constraints imposed on males by an expanded reproductive season (~ 6 months), resulting from the weak reproductive synchrony among females, and those imposed on females by a very long lactation period (at least one year but up to three years), may explain the limited mobility of adult Galapagos sea lions of both sexes across the archipelago.
Assuntos
Comportamento Alimentar , Isótopos/metabolismo , Leões-Marinhos/fisiologia , Animais , Equador , Feminino , MasculinoRESUMO
RATIONALE: The use of accurate, species-specific diet-tissue discrimination factors is a critical requirement when applying stable isotope mixing models to predict consumer diet composition. Thus, diet-to-female and female-to-pup isotopic discrimination factors in several tissues for both captive and wild South American sea lions were estimated to provide appropriate values for quantifying feeding preferences at different timescales in the wild populations of this species. METHODS: Stable carbon and nitrogen isotope ratios in the blood components of two female-pup pairs and females' prey muscle from captive individuals were determined by elemental analyzer/isotope ratio mass spectrometry (EA/IRMS) to calculate the respective isotopic discrimination factors. The same analysis was carried out in both blood components, and skin and hair tissues for eight female-pup pairs from wild individuals. RESULTS: Mean diet-to-female Δ(13) C and Δ(15) N values were higher than the female-to-pup ones. Pup tissues were more (15) N-enriched than their mothers but (13) C-depleted in serum and plasma tissues. In most of the tissue comparisons, we found differences in both Δ(15) N and Δ(13) C values, supporting tissue-specific discrimination. We found no differences between captive and wild female-to-pup discrimination factors either in Δ(13) C or Δ(15) N values of blood components. CONCLUSIONS: Only the stable isotope ratios in pup blood are good proxies of the individual lactating females. Thus, we suggest that blood components are more appropriate to quantify the feeding habits of wild individuals of this species. Furthermore, because female-to-pup discrimination factors for blood components did not differ between captive and wild individuals, we suggest that results for captive experiments can be extrapolated to wild South American sea lion populations.
Assuntos
Animais Lactentes/fisiologia , Isótopos de Carbono/análise , Comportamento Alimentar/fisiologia , Isótopos de Nitrogênio/análise , Leões-Marinhos/fisiologia , Animais , Isótopos de Carbono/sangue , Isótopos de Carbono/metabolismo , Dieta/veterinária , Feminino , Lactação , Isótopos de Nitrogênio/sangue , Isótopos de Nitrogênio/metabolismo , Alimentos Marinhos/análise , América do Sul , Distribuição TecidualRESUMO
There are not "universal methods" to determine diet composition of predators. Most traditional methods are biased because of their reliance on differential digestibility and the recovery of hard items. By relying on assimilated food, stable isotope and Bayesian mixing models (SIMMs) resolve many biases of traditional methods. SIMMs can incorporate prior information (i.e. proportional diet composition) that may improve the precision in the estimated dietary composition. However few studies have assessed the performance of traditional methods and SIMMs with and without informative priors to study the predators' diets. Here we compare the diet compositions of the South American fur seal and sea lions obtained by scats analysis and by SIMMs-UP (uninformative priors) and assess whether informative priors (SIMMs-IP) from the scat analysis improved the estimated diet composition compared to SIMMs-UP. According to the SIMM-UP, while pelagic species dominated the fur seal's diet the sea lion's did not have a clear dominance of any prey. In contrast, SIMM-IP's diets compositions were dominated by the same preys as in scat analyses. When prior information influenced SIMMs' estimates, incorporating informative priors improved the precision in the estimated diet composition at the risk of inducing biases in the estimates. If preys isotopic data allow discriminating preys' contributions to diets, informative priors should lead to more precise but unbiased estimated diet composition. Just as estimates of diet composition obtained from traditional methods are critically interpreted because of their biases, care must be exercised when interpreting diet composition obtained by SIMMs-IP. The best approach to obtain a near-complete view of predators' diet composition should involve the simultaneous consideration of different sources of partial evidence (traditional methods, SIMM-UP and SIMM-IP) in the light of natural history of the predator species so as to reliably ascertain and weight the information yielded by each method.