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Nat Prod Res ; 33(12): 1769-1772, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29390916

RESUMO

Astrocytic tumour cells derived from human (GL-15) and rat (C6) gliomas, as well as non-tumoural astrocytic cells, were exposed to the saponin-rich fraction (SF) from Agave sisalana waste and the cytotoxic effects were evaluated. Cytotoxicity assays revealed a reduction of cell viability that was more intensive in glioma than in non-tumoural cells. The SF induced morphological changes in C6 cells. They were characterised by cytoplasmic vacuole formation associated with increase in the formation of acidic lysosomes. The SF was subjected to purification on Sephadex LH-20, which characterised three probable steroidal saponins (sisalins) by electrospray ionisation mass spectrometry multistage (ESI-MSn). Sisalins from sisal may be responsible for the cytotoxicity, which involves cytoplasmatic vacuole formation and selective action for glioma cells.


Assuntos
Agave/química , Antineoplásicos Fitogênicos/farmacologia , Astrócitos/efeitos dos fármacos , Saponinas/química , Saponinas/farmacologia , Animais , Antineoplásicos Fitogênicos/química , Astrócitos/patologia , Linhagem Celular Tumoral , Chlorocebus aethiops , Citoplasma/efeitos dos fármacos , Citoplasma/patologia , Glioma/patologia , Humanos , Estrutura Molecular , Extratos Vegetais/química , Ratos , Saponinas/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem , Vacúolos/efeitos dos fármacos , Vacúolos/patologia , Células Vero
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