RESUMO
The Curtobacterium genus is a member of the family Microbacteriaceae, and Curtobacterium species are recognized as plant pathogens. The aim of this study was to investigate a dubious result of species identification for an infection located on a catheter tip of a patient with Covid-19. A strain isolated from a catheter tip sample, identified by VITEK® 2 as Cronobacter spp., was submitted to polyphasic analysis: Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) using VITEK® MS, real-time polymerase chain reaction targeting dnaG gene, and 16S rRNA full gene Sanger sequencing analysis for confirmation. The strain presented negative result using qPCR and could not identified by MALDI-TOF MS. 16S rRNA full gene Sanger sequencing analysis identified the strain as Curtobacterium spp. The Gram-variable characteristic (Gram-negative instead of Gram-positive) of the isolated strain was the responsible for the misidentification by VITEK® 2 and VITEK® MS did not identify the strain. 16S rRNA full gene sequencing analysis identified the strain as Curtobacterium genus, but other complementary techniques are necessary to identify at species level.
Assuntos
Actinomycetales , COVID-19 , Cronobacter , Actinomycetales/genética , Técnicas de Tipagem Bacteriana/métodos , Catéteres , Humanos , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodosRESUMO
The objectives of this study were (1) to determine the sensitivity (Se) and specificity (Sp) of somatic cell count (SCC) thresholds to identify subclinical mastitis in Gyr cows caused by major and minor pathogens; (2) to study the effects of month of sampling, rear or front mammary quarters, herd, intramammary infection (IMI), and bacterial species on SCC at quarter level; and (3) to describe the prevalence of IMI in Gyr cows in commercial dairy herds. In total, 221 lactating Gyr cows from 3 commercial dairy farms were selected. Milk samples were collected from individual quarters once a month for 1 yr from all lactating cows for SCC and bacteriological analysis. Mammary quarters were considered the experimental units and the SCC results were log(10)-transformed. Four SCC thresholds (100, 200, 300 and 400 × 10(3) cells/mL) were used to determine Se and Sp to identify infected mammary quarters. The overall prevalence of IMI in quarter milk samples of Gyr cows was 49.8%, and the prevalence of minor pathogens was higher (31.9%) than that of major pathogens (17.8%). Quarter samples with microbial isolation presented higher SCC compared with negative samples. Sensitivity and Sp of selected SCC thresholds varied according to the group of pathogen (major and minor) involved in the IMI definition. Sensitivity increased and Sp decreased when mammary quarters with only major pathogens isolation were considered positive. The use of a single SCC analysis to classify quarters as uninfected or infected in Gyr cows may not be a useful test for this breed because Se and Sp of SCC at the studied thresholds were low. The occurrence of IMI and the bacterial species are the main factors responsible for SCC variation in mammary quarters of Gyr cows. Milk samples with major pathogens isolation elicited higher SCC than those with minor pathogens.
Assuntos
Mastite Bovina/diagnóstico , Leite/citologia , Animais , Infecções Assintomáticas , Bovinos , Contagem de Células/veterinária , Indústria de Laticínios/métodos , Feminino , Mastite Bovina/epidemiologia , Mastite Bovina/microbiologia , Leite/microbiologia , Prevalência , Sensibilidade e EspecificidadeRESUMO
Fructose-1,6-bisphosphate (FBP) has been reported to have a protective effect on liver injury following ischemic/reperfusion periods. FBP maintains ATP levels and thereby cellular energy metabolism, which is important to the liver during cold preservation. In the present study, we evaluated the effects of FBP on the composition of storage solutions for cold liver preservation. Adult male Wistar rats were randomly divided into three experimental groups. Hepatic perfusion and preservation were performed with UW, UW plus 10 mmol/L FBP (UWM), and FBP 10 mmol/L (FBPS) alone solutions. Biochemical measurements of AST, ALT, and TBARS were performed on samples of the cold storage solution at 0, 12, 18, and 24 hours preservation. FBPS and UW solutions showed similar preservation grades during 18 hours. Addition of 10 mmol/L of FBP to UW solution induced liver injury and a poor preservation grade. FBP appears to protect the liver from injury caused by free radicals when the preservation time is less than 18 hours. Therefore, FBP may exert a protective effect for the preservation of livers during cold storage, and could represent an important component of new cold storage solutions.
Assuntos
Frutosedifosfatos , Transplante de Fígado/fisiologia , Fígado , Soluções para Preservação de Órgãos , Adenosina , Alanina Transaminase/análise , Alopurinol , Animais , Glutationa , Insulina , Fígado/fisiologia , Masculino , Rafinose , Ratos , Ratos Wistar , Substâncias Reativas com Ácido Tiobarbitúrico/análiseAssuntos
Alcalose/etiologia , Procedimentos Cirúrgicos Cardíacos , Soluções Isotônicas/efeitos adversos , Reação Transfusional , Dióxido de Carbono/sangue , Citratos , Ácido Cítrico , Eletrólitos/sangue , Circulação Extracorpórea , Feminino , Humanos , Masculino , Oxigênio/sangue , Período Pós-Operatório , Lactato de RingerAssuntos
Procedimentos Cirúrgicos Cardíacos , Digoxina/sangue , Circulação Extracorpórea , Adolescente , Adulto , Arritmias Cardíacas/etiologia , Digoxina/uso terapêutico , Circulação Extracorpórea/efeitos adversos , Feminino , Hematócrito , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Pré-MedicaçãoRESUMO
Evalúa la incidencia de las emisiones de plomo, como contaminante específico, sobre el medio ambiente y, en particular, sobre la salud de la población. Con datos suministrados por empresas nacionales que cubren el 33 por ciento de la producción total del país, se alimenta un modelo matemático-ecuación de Goldsmith y Hexter para relacionar la concentración de plomo en el aire y sus efectos en la salud. Se concluye que, comparando los valores de plomo estimados con las normas de
Assuntos
Argentina , Poluição do Ar , Poluentes Químicos , ChumboRESUMO
Evalúa la incidencia de las emisiones de plomo, como contaminante específico, sobre el medio ambiente y, en particular, sobre la salud de la población. Con datos suministrados por empresas nacionales que cubren el 33 por ciento de la producción total del país, se alimenta un modelo matemático-ecuación de Goldsmith y Hexter para relacionar la concentración de plomo en el aire y sus efectos en la salud. Se concluye que, comparando los valores de plomo estimados con las normas de
Assuntos
Argentina , Poluentes Químicos , Chumbo , Poluição do ArRESUMO
Evalúa la incidencia de las emisiones de plomo, como contaminante específico, sobre el medio ambiente y, en particular, sobre la salud de la población. Con datos suministrados por empresas nacionales que cubren el 33 por ciento de la producción total del país, se alimenta un modelo matemático-ecuación de Goldsmith y Hexter para relacionar la concentración de plomo en el aire y sus efectos en la salud. Se concluye que, comparando los valores de plomo estimados con las normas de