RESUMO
This paper reports the birth of a healthy baby resulting from transfer of blastocysts that were cryopreserved using propanediol after spontaneous hatching. A young infertile couple underwent IVF treatment in the clinic. After several IVF attempts, two births resulted; the first one with fresh embryos in 1996 after three IVF cycles, and the second one in 1999 (after a new IVF cycle in 1998) with frozen blastocysts that had remained cryopreserved in 1.5 mol/l propanediol and 0.1 mol/l sucrose after spontaneous hatching. This report of a healthy baby following transfer of hatched blastocysts frozen in propanediol supports further exploration of this approach.
Assuntos
Blastocisto/metabolismo , Criopreservação , Transferência Embrionária , Propilenoglicóis/metabolismo , Adulto , Feminino , Humanos , GravidezAssuntos
Humanos , Feminino , Gravidez , Masculino , Adulto , Fertilização in vitro/métodos , Criopreservação/métodos , Criopreservação/tendências , Zona PelúcidaAssuntos
Humanos , Feminino , Gravidez , Masculino , Adulto , Criopreservação/métodos , Fertilização in vitro/métodos , Criopreservação/tendências , Zona PelúcidaRESUMO
We report five cases in which no oocytes were retrieved after standard ovarian stimulation for in-vitro fertilization (IVF), and in which it was found that mistakes had been made at the time of human chorionic gonadotrophin (HCG) administration. In all five cases, oocyte retrieval was achieved after injecting HCG, when necessary, and reprogramming aspiration 24-36 h later. A mean of 7+/-3.2 MII oocytes were recovered per patient and 3.2+/-0.8 embryos were transferred. Three clinical pregnancies were obtained, and four healthy infants were born. In our programme, these were the only cases of empty follicle syndrome (EFS) that appeared over a total of 1118 cycles, and were all explained by human error in the administration of HCG. Our experience shows that human error could be considered a significant factor in the aetiology of empty follicle syndrome, and that EFS may be in part avoided by taking simple preventive measures.
Assuntos
Fertilização in vitro/efeitos adversos , Folículo Ovariano/citologia , Adulto , Gonadotropina Coriônica/administração & dosagem , Gonadotropina Coriônica/efeitos adversos , Transferência Embrionária , Feminino , Fertilização in vitro/métodos , Humanos , Recém-Nascido , Inalação , Masculino , Oócitos/citologia , Indução da Ovulação/efeitos adversos , Indução da Ovulação/métodos , Gravidez , Resultado da Gravidez , Síndrome , Fatores de TempoRESUMO
We have obtained a clinical pregnancy in a 26 year old patient by means of in vitro oocyte fertilization by intracytoplasmic injection of spermatozoa obtained from a cryopreserved testicular biopsy. In a first attempt performed with fresh biopsy material, the woman became pregnant, but the pregnancy ended in a spontaneous abortion. In a second cycle, the spermatozoa were retrieved from a cryopreserved sample saved from the first attempt. Twelve metaphase II oocytes were collected and injected; from these, nine became fertilized, three preembryos were transferred at the eighth-cell stage and the other six were cryopreserved. An ongoing clinical pregnancy was obtained with two gestational sacs.