RESUMO
The epithelium-melanin unit is formed by the melanocytes and keratinocytes. There is little information available about the behavior of melanocytes after surgical injury. Five white patients with comparable gingival pigmentation underwent gingivectomy to remove bandlike melanin pigmentations for cosmetic reasons. Biopsy specimens were taken from gingivectomy sites and healing areas 2, 3, 6, 7, 15, 50, and 180 days and 1.5, 3, and 5 years after the procedure. Transmission electron microscopic study revealed melanocytes in the process of migration and undergoing mitosis 6 and 7 days postoperatively. These cells exhibited, in the 15-day specimens, renewal of their dendritic processes and the four different stages of melanosome development. Keratinocytes were devoid of pigmented material until 50 days postoperatively. Clinically, the intensity of the pigmentation varied among the patients. Two reached baseline coloration 1.5 years postsurgery, while three returned to baseline coloration by 3 years postsurgery. Thus, gingival resective procedures, if performed solely for cosmetic reasons, offer no permanent results. (Int J Periodont Rest Dent 1993; 13:85-92.)
Assuntos
Doenças da Gengiva/fisiopatologia , Gengivectomia , Melaninas/fisiologia , Melanócitos/ultraestrutura , Melanose/fisiopatologia , Adulto , Divisão Celular , Movimento Celular , Doenças da Gengiva/cirurgia , Humanos , Queratinócitos/fisiologia , Queratinócitos/ultraestrutura , Estudos Longitudinais , Melanócitos/fisiologia , Melanose/cirurgia , Microscopia Eletrônica , Recidiva , CicatrizaçãoRESUMO
Analysis of lysosomes through acid phosphatase cytochemistry at the electron microscopy level has been performed in spleen and foot lesions from Leishmania-infected hamsters. The results showed that there is lysosomal depletion in macrophages from Leishmania donovani chagasi-infected hamster spleen and similar findings were obtained from foot lesions of Leishmania mexicana amazonensis-infected hamsters. The distribution of acid phosphatase and thiamine pyrophosphatase was also examined in the Golgi apparatus. It was possible to demonstrate that the activity of ACP is absent in infected macrophages from spleen and foot lesions of Leishmania-infected hamster while the distribution of TPP was very similar in control and infected macrophages from both systems. These results provide evidence that the lysosomal depletion can occur at the ACP synthesis and/or glycosylation level.
Assuntos
Leishmaniose Visceral/enzimologia , Leishmaniose/enzimologia , Lisossomos/enzimologia , Macrófagos/enzimologia , Fosfatase Ácida/análise , Animais , Células Cultivadas , Cricetinae , Complexo de Golgi/enzimologia , Histocitoquímica , Leishmania donovani/enzimologia , Leishmania donovani/ultraestrutura , Leishmania mexicana/enzimologia , Leishmania mexicana/ultraestrutura , Lisossomos/ultraestrutura , Macrófagos/ultraestrutura , Mesocricetus , Microscopia Eletrônica , Baço/enzimologia , Baço/parasitologia , Baço/ultraestrutura , Tiamina Pirofosfatase/análiseAssuntos
Intestinos/cirurgia , Derivação Jejunoileal , Pâncreas , Amilases/metabolismo , Análise de Variância , Animais , Peso Corporal , Lipase/metabolismo , Masculino , Tamanho do Órgão , Pâncreas/metabolismo , Pâncreas/patologia , Pâncreas/ultraestrutura , Proteínas/metabolismo , Ratos , Ratos EndogâmicosRESUMO
Female pregnant rats of 2BAW strain were divided in 2 groups: the 1st, received 50 mg/kg corporal weight of sodium 1-phenyl-2,3-dimethyl-5-pyrazolon-4-methylamino-methane sulfonate (Dipyrone), single dose daily, by i.p. injections, from 16th to 20th day of pregnancy; the 2nd, received 0,5 ml of distilled water, single dose daily, by i.p. injections, during the same period. All the animals were sacrificed 2 hours after the last injection. The biochemical results of nucleic acids in the placentas and fetal livers, and the caryometric data of trophoblastic giant cells and fetal hepatocytes, demonstrated that: 1. When compared the 2 groups, as much the nucleic acids levels (RNA and DNA) of placentas as the nuclear size of trophoblastic giant cells, do not presented statistical differences; 2. The biochemical levels of nucleic acids (RNA and DNA) of fetal livers decreased, while the nuclear size of hepatocytes increased in the experimental group, with reference to control group.