RESUMO

BACKGROUND Bone marrow stem cells have been shown to be a promising therapeutic strategy for autoimmune diseases. This study aimed to assess the safety and efficacy of autologous hematopoietic stem cell (ABMSC) transplantation without immunoablation used to suppress the autoimmune reaction in 6 children with newly diagnosed autoimmune diabetes mellitus. We monitored the levels of islet cell antibodies (ICA), antibodies against islet antigen-related tyrosine phosphatase 2 (IA2), glutamic acid-decarboxylase (GAD) antibodies, and anti-insulin antibodies (AIA). MATERIAL AND METHODS Between 2018 and 2022, 6 children (age 6-10 years, average 8 years) recently diagnosed with type 1 diabetes mellitus with the presence of ICA, IA2, GAD, AIA and ketoacidosis, were treated with an ABMSC stimulated with Filgrastim, granulocyte colony-stimulating factor (G-CSF), 10 ug/kg/day for 4 days. Bone marrow was harvested on day 5, collected by puncture and identified as mononuclear cells >180×106/kg, CD34+ >0.22%, and transplanted by intravenous (i.v.) infusion. Patients were monitored with ICA, IA2, GAD, AIA, C-peptide, blood glucose, and glycosylated hemoglobin A1c (HbA1C) 6 months after the procedure. RESULTS At 6-month follow-up, we observed a negative value of the ICA, which was previously positive (P<0.001). The IA2 (p=0.037) and GAD (P=0.377) antibodies decreased slowly but were significantly lower. AIA remained high. A decrease in blood glucose and HbA1C levels was observed (P<0.001). No complications occurred during follow-up. CONCLUSIONS Autologous hematopoietic stem cell transplantation without immunoablation was safe and effective in significantly decreasing the production and effect of autoantibodies against ICA, GAD, and IA2, as well as decreasing blood sugar levels and HbA1c.

Assuntos

RESUMO

BACKGROUND: Scarless/single-incision laparoscopic cholecystectomy (SILC) is a new procedure. It affords a superior cosmetic outcome when compared to conventional laparoscopic cholecystectomy. We examine the application of this technique using LigaSure via a clipless method. The present study looks at the experience of a single surgeon using this method with initial evaluation of the safety, feasibility, affordability, and benefits of this procedure. METHODS: Twenty-eight patients underwent transumbilical SILC at Doctors Hospital from January to December, 2014. The cohort included both emergency and elective patients. There was no difference in the preoperative work-up as indicated. To perform the operation, a 2-2.5-cm linear incision was made through the umbilicus and the single port platform utilized. A 10mm 30-degree laparoscope, a 5mm LigaSure and straight instruments were used to perform the laparoscopic cholecystectomy procedure. RESULTS: All patients except two were operated on successfully. Conversion was considered the placement of an additional epigastric/Right upper quadrant (RUQ) port. The conversion rate to standard LC was 7%. No patient was converted to open cholecystectomy. In the 28 successfully completed patients, the median duration of the operation was 38.5min and estimated operative blood loss was 24ml. Patients were commenced on liquid diet immediately on being fully conscious and after return to the ward with an estimated time of 6h. The mean postoperative hospital stay was 1.4 days. Follow-up visits were conducted for all patients at 2-weeks intervals and continued for 6 weeks after surgery where possible. Two patients developed wound infections. All patients were satisfied with the good cosmetic effect of the surgery. The total satisfaction rate was 100%. CONCLUSIONS: SILC is a safe and feasible technique for operating with scarless outcomes and reducing perioperative discomfort at the same time. The GelPOINTTM is a safe and feasible platform to be used. The procedure can be accomplished using regular instruments and laparoscope. Curved instruments and a bariatric length laparoscope may make the procedure easier and result in greater time saving. The addition of LigaSure™ decreases the complexity of the operation, decreases operative time and blood loss. The technique is economical in a resource-limited environment.

RESUMO

Antibiotics do have an important role to play in the prophylaxis of infections in the surgical patient. However this frequently result in their inapproaptiate use since little/no criteria are adhered to in their application. Few surgeons avoid the temptation of abusing antibiotics as "cleansing agents" in surgical situations where they would be hard pressed to support the necessity of such application by their, or any other study. Yet the controversy ranges; antibiotics continue to be abused; resistants continue to be selected out and nosocomial sepsis continues to be more difficult and more costly to treat. The antibiotics regimen outline in this article is one of many available to the surgeon. The purpose of this article is not to present a protocol, but to stimulate cooperation between the microbiologist, infectious disease control presonnel and the surgeon. Then, changing bacterial sensitivity patterns may be recognized, and antibiotics institued appropriately based on strict, feasible criteria for established and /or anticipated nosocomial sepsis (AU)

Assuntos

RESUMO

Antibotics do have an important role to play in the prophylaxis of infections in the surgical patient. However this frequently results in their inappropriate use since little/no criteria are adhered to in their application. Few surgeons avoid the temptation of abusing antiobiotics as "cleansing agents" in surgical situations where they would be hard pressed support the necessity of such application by their, or any study. Yet the controversy ranges; antibiotics continue to be abused; resistant bacterial strains continue to be selected and nosocomial sepsis continues to be more difficult and more costly to treat. The antibiotics regimen outlined in this article is one of many available to the surgeon. The purpose of this article is not to present a protocol, but to stimulate cooperation between the microbiologist, infectious disease control personnel and the surgeon. Then, handling bacterial sensitivity patterns may recognized, and antibiotics instituted appropriately based on strict, feasible criteria for established and/or anticipated nosocomial sepsis(AU)

Assuntos