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1.
Bio Protoc ; 11(22): e4222, 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34909443

RESUMO

This protocol describes a method for high-resolution confocal imaging of pericytes with the far-red fluorophore TO-PROTM-3 Iodide 642/661 in cerebral slices of murine. Identification of pericytes with TO-PRO-3 is a short time-consuming, high cost-effective and robust technique to label pericytes with no need for immunostaining or generation of reporter mice. Since the TO-PRO-3 stain resists immunofluorescence, and lacks spectral overlap, the probe is well suited for multiple labelling. Our procedures also combine TO-PRO-3-staining of pericytes with fluorescent markers for astrocytes and vessels in brain slices. These approaches should enable the assessment of pericyte biology in gliovascular unit.

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In. Universidad de la República. Facultad de Medicina. Hospital de Clínicas Dr. Manuel Quintela. Proyectos de investigación. Montevideo, Udelar, sept.2011. p.16-16.
Monografia em Espanhol | BVSNACUY | ID: bnu-15963
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