RESUMO

A comparison was made between the conjugate of national production made by "Carlos J. Finlay" Enterprise of Biological Products for diagnosing rabies by direct immunofluorescence and the viral antinucleocapsid conjugate manufactured by BIORAD that is commercialized at the international level. 150 samples of brain from different animal species were studied at the Rabies Reference Laboratory of "Pedro Kouri" Institute of Tropical Medicine from 2000 to 2002. On comparing both conjugates, there were obtained sensibility, specificity and concordance values of 100%, 94.3% and 98%, respectively. The discordant results were analyzed by the polymerase chain reaction and the biological test in mice.

Assuntos

Anticorpos Antivirais/análise , Encéfalo/virologia , Técnica Direta de Fluorescência para Anticorpo/veterinária , Vírus da Raiva/isolamento & purificação , Raiva/veterinária , Animais , Anticorpos Monoclonais/imunologia , Gatos , Bovinos , Quirópteros , Cuba , Cães , Ensaio de Imunoadsorção Enzimática/veterinária , Herpestidae , Camundongos , Proteínas do Nucleocapsídeo/imunologia , Reação em Cadeia da Polimerase/veterinária , Raiva/diagnóstico , Vírus da Raiva/imunologia , Roedores , Sensibilidade e Especificidade , Ovinos , Suínos