RESUMO
The antiviral activity of butanol- and acetic acid-soluble fractions, prepared from the leaves and stems of Phyllanthus orbicularis H.B.K., has been investigated against acyclovir-sensitive or -resistant herpes simplex virus type 1 (HSV-1) strains, using human foreskin fibroblast (HFF) and green ape kidney (Vero) cell lines. Both fractions showed antiviral selectivity indexes (SI) from 10.3 to 22.8, while their extracellular virucidal activities reached SI values ranging from 371 to 1,040. Time-addition experiments suggested that the active compounds present in the studied fractions acted on early steps of the virus replication cycle.
Assuntos
Antivirais/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Phyllanthus , Fitoterapia , Extratos Vegetais/farmacologia , Aciclovir/farmacologia , Animais , Antivirais/administração & dosagem , Antivirais/uso terapêutico , Chlorocebus aethiops , Fibroblastos/efeitos dos fármacos , Humanos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Células Vero/efeitos dos fármacosRESUMO
Three species from the Phyllanthus genus coming from the Cuban eastern zone were studied to determine the inactivation capacity of the surface antigen (Ags HB) of in vitro hepatitis B virus. Alcoholic extracts were prepared from each species and from different parts of such plants, and sera from patients positive to Ags HB were treated with them. Results demonstrate that the analysed species own the capacity of inactivating that antigen between the 93 and the 97% of the sera assayed. The inactivation capacity analysis of the three parts of Phyllanthus chamaecristoides revealed a greater activity in extracts from the stems (97%) with a behavior resembling the two incubation temperatures used. The presence of flavonoids in the extract of this species is observed.