RESUMO
Los avances en la tecnología del DNA recombinante han aumentado la utilidad potencial de las líneas celulares continuas como sustratos para la propagación y producción de antígenos utilizados como vacunas. Sin embargo, han surgido dudas sobre la inocuidad de los productos biológicos producidos con estos métodos. Un Grupo de Estudio de la OMS concluyó que, en general, las líneas celulares continuas de mamífero son sustratos aceptables para la fabricación de productos biológicos y que su uso debe considerarse individualmente y estar sometido a vigilancia continua
Disponible en inglés en Bull. WHO 62(2), 1991
Assuntos
Vacinas Atenuadas , Vacinas Virais , Qualidade de Produtos para o Consumidor , DNA Recombinante , Cultura de Vírus , Produtos BiológicosRESUMO
To determine the membrane properties in T-lymphocyte population from Saguinus primates or lymphoblastoid cell lines of the same origin, an allotypic antiserum was prepared inoculating two Saguinus illigeri with 3.5 x 10(10) viable 70N2 cells obtained in-vitro, from a lymphoma induced by inoculation of Herpesvirus Saimiri in a Saguinus oedipus. The 70N2 cells carried the virus genome in an episomal way; it did not produce infectious virus and it formed spontaneous rosettes (99%) with sheep erythrocytes. The resulting allotypic antiserum inhibited the formation of E rosettes and specifically reacted with lymphoblastic T-cell lines and peripheral lymphocytes of Saguinus oedipus while it did not cross react with these cells or peripheral lymphocytes of S. illigeri or nigrifons, S. jacchus, Saimiri sciureus , Papio Sp. and human primates. These results show that two antigenic populations exist within Saguinus T-lymphocytes; one is common to all members of the species and the other is specific for subspecies related to the spontaneous rosette receptors and it is present in lymphoblastic cell lines-T of the same origin.
Assuntos
Antígenos de Superfície/imunologia , Callitrichinae/imunologia , Saguinus/imunologia , Linfócitos T/classificação , Animais , Epitopos/imunologia , Isoanticorpos/imunologia , Masculino , Formação de Roseta , Especificidade da Espécie , Linfócitos T/imunologiaRESUMO
To determine the membrane properties in T-lymphocyte population from Saguinus primates or lymphoblastoid cell lines of the same origin, an allotypic antiserum was prepared inoculating two Saguinus illigeri with 3.5 x 10(10) viable 70N2 cells obtained in-vitro, from a lymphoma induced by inoculation of Herpesvirus Saimiri in a Saguinus oedipus. The 70N2 cells carried the virus genome in an episomal way; it did not produce infectious virus and it formed spontaneous rosettes (99
) with sheep erythrocytes. The resulting allotypic antiserum inhibited the formation of E rosettes and specifically reacted with lymphoblastic T-cell lines and peripheral lymphocytes of Saguinus oedipus while it did not cross react with these cells or peripheral lymphocytes of S. illigeri or nigrifons, S. jacchus, Saimiri sciureus , Papio Sp. and human primates. These results show that two antigenic populations exist within Saguinus T-lymphocytes; one is common to all members of the species and the other is specific for subspecies related to the spontaneous rosette receptors and it is present in lymphoblastic cell lines-T of the same origin.