RESUMO
Human purine nucleoside phosphorylase (HsPNP) catalyzes reversible phosphorolysis of nucleosides and deoxynucleosides in the purine cascade. HsPNP has been a target on behalf of the development of new leads for the treatment of a variety of T-cell mediated disorders. Several studies on the HsPNP are focused on the identification of effective, safe, and selective inhibitors. Therefore, this study describes the development of direct, simple, reliable, and inexpensive enzymatic assays to screen HsPNP inhibitors. Initially, HsPNP was covalently immobilized on the surface of magnetic particles (MPs). Due to the versatility of the MPs as solid support for enzyme immobilization, two different methods to monitor the enzyme activity are presented. Firstly, the activity of HsPNP-MPs was assessed offline by HPLC-DAD quantifying the formed hypoxanthine. Then, HsPNP-MPs were trapped in a peek tube, furnishing a microreactor which was inserted on-flow in an HPLC-DAD system to monitor the enzyme activity by the hypoxanthine quantification. Kinetic assays provided KMapp values for the inosine substrate of 488.2 ± 49.1 and 1084 ± 111 µM for the offline and on-flow assays, respectively. For the first time, kinetic studies for Pi as substrate using the HsPNP-MPs exhibits a Michaelis-Menten kinetic, yielding KMapp values for offline and on-flow of 521.2 ± 62.9 µM and 601 ± 66.5 µM, respectively. Inhibition studies conducted with a fourth generation immucillin derivative (DI4G) were employed as proof of concept to validate the use of the HsPNP-MPs assays for screening purposes. Additionally, a small library containing 11 compounds was used to assess the selectivity of the developed assays. The results showed that both presented assays can be applied to selectively recognizing and characterizing HsPNP inhibitors. Particularly, the on-flow method exhibited a high throughput and performance because of its automation and represents an easy and practical approach to reuse the HsPNP-MPs. Besides, this novel enzyme activity assay model can be further applied to other biological targets.
Assuntos
Fenômenos Magnéticos , Purina-Núcleosídeo Fosforilase , Inibidores Enzimáticos/farmacologia , Humanos , Cinética , Nucleosídeos de Purina , Purina-Núcleosídeo Fosforilase/metabolismoRESUMO
Our aim was to give a morphometric description of the interproximal bone between the anterior maxillary teeth of subjects with class III facial deformity, who were candidates for segmented Le Fort I osteotomy. We measured the width of the interproximal bone from the upper right canine to the upper left canine in cone-beam computed tomographic images of 35 subjects, and identified five interproximal areas of measurement. The lower and upper measurements were established 5mm and 10mm from the cervical crest of the interproximal bone. A paired samples t test and Pearson's correlation coefficient were applied and probabilities of less than 0.05 were accepted as significant. In all the scans of interproximal bone, the apical zone was significantly wider than the inferior zone (p<0.001). The area between the central incisors was the widest, with a mean (SD) of 2.42 (0.68) mm in the lower, and 4.27 (0.99) mm in the upper, region followed by the space between the canines and lateral incisors. The minimum interproximal spaces in the lowest area were between 1.1 and 1.5mm, which suggested the potential for damage to the teeth during segmental osteotomy. The interproximal spaces were at potential risk of dental and periodontal injuries, and the area between the central incisors seemed to be most suited to interproximal osteotomies in "surgery first".
Assuntos
Osteotomia Maxilar , Osteotomia de Le Fort , Processo Alveolar , Tomografia Computadorizada de Feixe Cônico , Humanos , MaxilaRESUMO
This study aimed to describe the condylar layer and cartilage island in subjects with unilateral condylar hyperplasia (UCH). Five individuals (15-18 years old) with a diagnosis of UCH, treated in a university hospital in Temuco, Chile, were included. The analysis examined the presence, extension, and thickness of the layers on the condylar surface, the number, depth, and area of the cartilage islands, and the argyrophilic proteins of the nucleolar organizer region (AgNOR) score. Statistical significance was set at P<0.05. The fibrocartilaginous layer was thickest (0.13±0.05mm) and the joint layer was thinnest (0.07±0.01mm) (P<0.05). With respect to the number, depth, and area of the islands, case 1 presented the highest values, followed by case 2; the cartilage island was related to the fibrocartilaginous layer (P<0.05). All cases had AgNOR proteins in the proliferative and fibrocartilaginous layers, as well as the islands with the greatest presence of chondrocytes (P=0.245). A relationship was observed between the histopathological alterations in the different layers on the condylar surface and the thickness of the fibrocartilaginous layer, as well as the thickness of the latter and the number, depth, and area of the cartilage islands in the trabecular bone.
Assuntos
Cartilagem Articular/patologia , Má Oclusão Classe III de Angle/patologia , Côndilo Mandibular/patologia , Transtornos da Articulação Temporomandibular/patologia , Adolescente , Cartilagem Articular/diagnóstico por imagem , Cartilagem Articular/cirurgia , Tomografia Computadorizada de Feixe Cônico , Feminino , Humanos , Hiperplasia/diagnóstico por imagem , Hiperplasia/patologia , Hiperplasia/cirurgia , Masculino , Má Oclusão Classe III de Angle/diagnóstico por imagem , Má Oclusão Classe III de Angle/cirurgia , Côndilo Mandibular/diagnóstico por imagem , Côndilo Mandibular/cirurgia , Transtornos da Articulação Temporomandibular/diagnóstico por imagem , Transtornos da Articulação Temporomandibular/cirurgia , Tomografia Computadorizada de Emissão de Fóton Único , Resultado do TratamentoRESUMO
OBJECTIVE: Zygomatic implants may be used for dental rehabilitation in atrophic maxillae. The aim of this study was to establish experimentally the areas of stress distribution using 2 kinds of "All-On-Four" maxillary procedures. STUDY DESIGN: The best position to insert the implants was selected using polyurethane craniomaxillary models and surgical guides were made. Group 1 was designed with two posterior zygomatic implants and two conventional anterior implants, and group 2 with two posterior zygomatic implants and two anterior zygomatic implants. A titanium bar was built to link the 4 implants in both groups. Photoelastic replicas of these models were made and the implants were inserted using the surgical guides. An Instrom 4411 testing machine was used to perform a unilateral compressive loading at the level of the right first molar until 2mm of displacement was obtained. RESULTS: Group 1 showed a high strain concentration in the right lateral orbital region at the level of the apex of the zygomatic implant. Less strain was noticed at the apical levels of the conventional implants in the anterior sector and of the contralateral zygomatic implant. Group 2 showed high strains in the lateral inferior orbital area. The load was low in the alveolar bone sector. DISCUSSION: Zygomatic bone and paranasal structures are loaded at high levels when zygomatic implants are used to stabilize a full maxillary prosthodontic rehabilitation on 4 implants. The use of 4 zygomatic implants loads the alveolar bone to a lower extent and seems better suited from a mechanical point of view than the use of 2 zygomatic implants.
Assuntos
Implantação Dentária Endóssea/instrumentação , Implantação Dentária Endóssea/métodos , Prótese Dentária Fixada por Implante , Maxila/cirurgia , Estresse Mecânico , Zigoma/cirurgia , Fenômenos Biomecânicos , Planejamento de Prótese Dentária , Prótese Dentária Fixada por Implante/efeitos adversos , Humanos , Arcada Edêntula/cirurgia , Teste de Materiais/métodosRESUMO
The Nasal Obstruction Symptom Evaluation (NOSE) scale is a reliable and valid instrument used widely in otorhinolaryngology to evaluate nasal obstruction symptoms in patients with nasal disorders. The purpose of this study was to assess nasal obstruction symptoms prospectively in patients undergoing surgically assisted rapid maxillary expansion (SARME) using the NOSE scale. Sixteen patients were studied (mean age 31±7.7 years), 10 women and six men, all with a transverse maxillary deficiency and an indication for SARME. Hyrax type devices were placed preoperatively and SARME was performed using Kraut's technique. The NOSE scale was applied prospectively to assess nasal obstruction symptoms. The results were recorded for each score on a scale ranging from 0 to 4, and these scores were multiplied by 5, generating a balanced scale from 0 to 100. Data were stratified according to NOSE scores, and nasal obstruction was categorized as mild (0-25), moderate (26-50), or severe (>50). The questionnaire was administered twice, first preoperatively and then at 6 months after surgery, and the results compared. Data were analyzed statistically using SAS statistical package software and showed that patients experienced a subjective improvement or did not have a worsening of nasal obstruction symptoms after SARME.
Assuntos
Obstrução Nasal/diagnóstico , Obstrução Nasal/prevenção & controle , Técnica de Expansão Palatina , Adulto , Feminino , Humanos , Masculino , Estudos Prospectivos , Índice de Gravidade de Doença , Inquéritos e Questionários , Resultado do TratamentoRESUMO
The aim of this research was to compare the condylar morphology of patients with unilateral condylar hyperplasia (UCH) and patients with a class III skeletal relationship using cone beam computed tomography (CBCT). A prospective study was conducted on patients with facial asymmetry attending the division of oral and maxillofacial surgery of the study university in Chile. Fifteen patients with UCH and 15 with a class III skeletal relationship were selected. Linear measurements of the condylar processes were obtained at a scale of 1:1 using the software Ez3D Viewer Plus. Analysis of variance (ANOVA) and the paired t-test were used, considering P<0.05. Patients with UCH presented statistical differences between the hyperplastic condyle and non-hyperplastic condyle for anteroposterior and mediolateral diameters, condylar neck length, and ramus height. Patients with a class III skeletal relationship showed no differences between the right and left sides; the morphology of their condyles was similar to the condyles with hyperplasia and presented statistical differences when compared with the non-hyperplastic condyles (one-way ANOVA, P<0.05). The condylar morphology of UCH patients could be related to the development of a class III skeletal relationship. These findings provide an insight into the possibility of some class III patients presenting bilateral condylar hyperplasia.
Assuntos
Tomografia Computadorizada de Feixe Cônico , Assimetria Facial/diagnóstico por imagem , Má Oclusão Classe III de Angle/diagnóstico por imagem , Côndilo Mandibular/diagnóstico por imagem , Adolescente , Adulto , Assimetria Facial/patologia , Feminino , Humanos , Hiperplasia/diagnóstico por imagem , Hiperplasia/patologia , Masculino , Má Oclusão Classe III de Angle/patologia , Côndilo Mandibular/patologia , Estudos Prospectivos , Interpretação de Imagem Radiográfica Assistida por ComputadorRESUMO
Biflorin is an o-naphthoquinone isolated from the roots of the plant Capraria biflora L. (Scrophulariaceae). In this study, the cytotoxic effects of biflorin were verified, and late apoptosis was detected in various cancer cell lines by in situ analysis. The cytotoxicity was further evaluated exclusively for 48 h of treatment in different tumor and non-tumor cell lines (Hep-2, HeLa, HT-29, A-375, and A-549, and HEK-293, respectively). The results indicated that biflorin induced selective cytotoxicity in tumor cells. HeLa cells were more susceptible to biflorin, followed by HT-29, A-549, A-375, and Hep-2 at all concentrations (range 5-50 µg/mL), and the highest half-maximal inhibitory concentration IC50 (56.01 ± 1.17 µg/mL) was observed in HEK-293 cells. Late apoptotic/necrotic events, observed by in situ immunostaining with Annexin V, varied with each cell line; an increase in late apoptotic events was observed corresponding to the increase in biflorin dosage. Hep-2 cells showed a greater percentage of late apoptotic events among the tumor cell lines when treated with higher concentrations of biflorin (69.63 ± 2.28%). The non-tumor HEK-293 line showed greater resistance to late apoptotic events, as well as a lower level of cytotoxicity (77.69 ± 6.68%) than the tested tumor lines. The data presented indicate that biflorin showed an important, possibly selective, cytotoxicity against tumor cell lines, thereby revealing a promising novel substance with potential anticancer activity for tumor therapy.
Assuntos
Antineoplásicos Fitogênicos/administração & dosagem , Naftoquinonas/administração & dosagem , Neoplasias/tratamento farmacológico , Antineoplásicos Fitogênicos/química , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Células HEK293 , Humanos , Naftoquinonas/química , Neoplasias/patologia , Scrophulariaceae/químicaRESUMO
Assessments of chromosomal integrity and structure enable the prevention of diseases associated with the work environment, with the frequencies of chromosomal aberrations and micronuclei often being used as markers in biomonitoring. Owing to their routine manipulation of potentially toxic chemicals, tannery workers as a group merit a more thorough evaluation and discussion. This study investigated chromosomal damage in 30 workers from a tannery in the city of Teresina, the state capital of Piauí, northeast Brazil, and a control group consisting of 30 employees from a nearby accounting firm. The frequencies of chromosomal aberrations (CAs) and binucleated cell micronuclei (MN) were assessed as a measure of damage. Means were compared using the Student t-test and ANOVA-Dunnett test. Our results indicated a higher number of CAs in exposed individuals compared to the control group, including dicentric (P < 0.0001) and tricentric chromosomes (P < 0.001), and those in ring (P < 0.0001) and acentric ring forms (P < 0.001). Assessment of MN frequency demonstrated a similar trend (exposed vs control, P < 0.0001). It was concluded that the tannery workers in this study exhibited a higher incidence of genetic damage than comparable unexposed individuals. However, further research on this subject is needed, particularly in regard to potentially clastogenic agents used in the tanning process.
Assuntos
Aberrações Cromossômicas/efeitos dos fármacos , Mutagênicos/toxicidade , Exposição Ocupacional , Curtume , Adolescente , Adulto , Idoso , Brasil , Estudos de Casos e Controles , Análise Citogenética , Feminino , Humanos , Masculino , Micronúcleos com Defeito Cromossômico , Testes para Micronúcleos , Pessoa de Meia-IdadeRESUMO
OBJECTIVE: To assess the effect of dimeticone and pepsin on the bioavailability of metoclopramide (CAS 7232-21-5) in healthy volunteers. METHODS: The study was conducted using a randomized, open, 2-period crossover design. The volunteers received single administration of 7-mg conventional metoclopramide capsule and a formulation containing metoclopramide (7 mg) plus dimeticone (40 mg) and pepsin (50 mg), with a 7-day interval between treatments. Serial blood samples were collected before dosing and during 24 h post-treatment. Plasma metoclopramide concentrations were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The pharmacokinetics parameters AUC(last) and C(max) were obtained from the metoclopramide plasma concentration vs. time curves. RESULTS: Metoclopramide's association was bioequivalent to conventional capsule; 90% CIs for geometric mean treatment ratios of C(max) [108.0% (90% CI, 100.4-116.3%)], AUC(last) [103.3% (90% CI, 99.5-107.4%)] were within the predefined range. The metoclopramide formulations were well tolerated at the administered doses and no significant adverse reactions were observed. Thus, these results confirm the good bioavailability of metoclopramide in the new formulation and rule out any impaired absorption when the drugs are formulated in combination.
Assuntos
Dimetilpolisiloxanos/administração & dosagem , Metoclopramida/farmacocinética , Pepsina A/administração & dosagem , Administração Oral , Adolescente , Adulto , Área Sob a Curva , Disponibilidade Biológica , Brasil , Cromatografia Líquida de Alta Pressão , Estudos Cross-Over , Combinação de Medicamentos , Feminino , Voluntários Saudáveis , Humanos , Masculino , Taxa de Depuração Metabólica , Metoclopramida/administração & dosagem , Metoclopramida/sangue , Pessoa de Meia-Idade , Comprimidos , Espectrometria de Massas em Tandem , Adulto JovemRESUMO
This study was a comparative evaluation of the bending resistance of metallic and resorbable plates and screws in a mandibular body fracture model. Forty polyurethane synthetic hemimandibles were used; a vertical linear cut was made between the second and first premolars. These 40 hemimandibles were divided into four groups of 10 and were fixed with titanium plates and screws or resorbable plates and screws, with monocortical screws in the upper sector and bicortical screws in the lower sector. Bending resistance tests were done on a universal testing machine with a linear displacement speed of 1mm/min, a cell load of 500 N, and a load cell on the lower central incisor or on the lower second premolar. Results were analyzed using the Student's t-test, with the significance level set at 5%. No statistically significant differences were observed between the groups studied, either in the analysis of the osteosynthesis materials or related to the load-bearing points. The variables of displacement and peak load did not present any significant differences. In this in vitro model of a mandibular body fracture, the mechanical behaviour of a resorbable osteosynthesis was similar to that of a titanium osteosynthesis.
Assuntos
Fixação Interna de Fraturas/instrumentação , Fraturas Mandibulares/cirurgia , Implantes Absorvíveis , Placas Ósseas , Parafusos Ósseos , Humanos , Técnicas In Vitro , Ácido Láctico , Teste de Materiais , Modelos Anatômicos , Osseointegração , Poliésteres , Polímeros , Estresse Mecânico , TitânioRESUMO
Partially edentulous patients may be rehabilitated by the placement of removable dental prostheses, implant-supported removable dental prostheses, or partial implant fixed dental prostheses. However, it is unclear the impact of each prosthesis type over the masticatory aspects, which represents the objective of this paired clinical trial. Twelve patients sequentially received and used each of these 3 prosthesis types for 2 months, after which maximum bite force was assessed by a strain sensor and food comminution index was determined with the sieving method. Masseter and temporal muscle thicknesses during rest and maximal clenching were also evaluated by ultrasonography. Each maxillary arch received a new complete denture that was used throughout the study. Data were analyzed by analysis of variance for repeated measures, followed by the Tukey test (p < .05). Maximum bite force and food comminution index increased (p < .0001) after implant-supported dental prosthesis and implant fixed dental prosthesis use, with the higher improvement found after the latter's use. Regardless of implant-retained prosthesis type, masseter muscle thickness during maximal clenching also increased (p < .05) after implant insertion. Partial implant-supported prostheses significantly improved masseter muscle thickness and mastication, and the magnitude of this effect was related to prosthesis type.
Assuntos
Prótese Dentária Fixada por Implante , Prótese Parcial , Mastigação/fisiologia , Idoso , Idoso de 80 Anos ou mais , Força de Mordida , Ligas de Cromo/química , Grampos Dentários , Implantação Dentária Endóssea , Implantes Dentários , Oclusão Dentária Balanceada , Planejamento de Dentadura , Retenção de Dentadura , Prótese Total Superior , Prótese Parcial Fixa , Prótese Parcial Removível , Feminino , Alimentos , Humanos , Arcada Parcialmente Edêntula/reabilitação , Masculino , Músculo Masseter/diagnóstico por imagem , Músculo Masseter/patologia , Pessoa de Meia-Idade , Contração Muscular/fisiologia , Osseointegração/fisiologia , Músculo Temporal/diagnóstico por imagem , Músculo Temporal/patologia , UltrassonografiaRESUMO
BACKGROUND: Lipids are required for mice sensitization to Ber e 1, Brazil nut major allergen. Here, we characterized different lipid fractions extracted from Brazil nuts and the lipid-binding ability of Ber e 1. Further, we determined their in vivo ability to induce Ber-specific anaphylactic antibodies and the role of invariant natural killer T (iNKT) cells in this process. METHODS: Wild-type (WT) and iNKT cell-deficient mice were sensitized with Ber e 1 and specific lipid fractions, and anaphylactic antibodies were measured by enzyme-linked immunosorbent assay (ELISA) and passive cutaneous anaphylaxis (PCA). The lipid-binding characteristic of Ber e 1 (Ber) was established by using fluorescent probes and (15) N-labeled NMR. In vitro production of IL-4 was determined in Ber/lipid C-stimulated mouse iNKT cells and human T-cell lines containing NKTs primed with CD1d+C1R transfectants by flow cytometry and ELISA, respectively. RESULTS: Only one specific lipid fraction (lipid C), containing neutral and common phospholipids, induced Ber anaphylactic antibodies in mice. Ber e 1 has a lipid-binding site, and our results indicated an interaction between Ber e 1 and lipid C. iNKT-deficient mice produced lower levels of anaphylactic antibodies than WT mice. In vitro, Ber/lipid C-stimulated murine iNKT cells produced IL-4 but not IFN-gamma. Human T-cell lines derived from nut-allergic patients produced IL-4 to Ber/lipid C in a CD1d- and dose-dependent manner. CONCLUSION: Lipid fraction C from Brazil nut presents an essential adjuvant activity to Ber e 1 sensitization, and iNKT cells play a critical role in the development of Brazil nut-allergic response.
Assuntos
Lipídeos/imunologia , Células T Matadoras Naturais/imunologia , Hipersensibilidade a Noz/imunologia , Albuminas 2S de Plantas/química , Albuminas 2S de Plantas/imunologia , Adolescente , Adulto , Alérgenos/imunologia , Animais , Anticorpos/sangue , Anticorpos/imunologia , Antígenos de Plantas/química , Antígenos de Plantas/imunologia , Sítios de Ligação , Feminino , Humanos , Interações Hidrofóbicas e Hidrofílicas , Ativação Linfocitária/imunologia , Masculino , Camundongos , Hipersensibilidade a Noz/metabolismo , Ligação Proteica , Células Th2/imunologia , Adulto JovemRESUMO
The aim of this study was to compare the mechanical stress over hemimandible substrate and hardware after sagittal split ramus osteotomy (SSRO) fixed with five different techniques using three-dimensional (3D) finite element analysis. A 3D finite element model of a hemimandible was created and a 5mm advancement SSRO was simulated on a computer model. The model was fixed with five different techniques: 3 linear 60° screw arrangement; 3 linear 90° screw arrangement; 3 inverted L screw arrangement; 1 conventional miniplate; and 1 locking miniplate with four monocortical screws. Load was applied until 3mm displacement was reached and the results were compared with previous mechanical and photoelastic tests, thus analysing the mechanical stresses developed in the proximity of miniplates and screws and within the fixation system itself. The maximum principal stress values demonstrate a lower mechanical stress rate in bone and in the fixation system with the inverted L arrangement, followed by the linear 90° and linear 60° arrangements. The locking miniplate/screw system presented lower maximum principal stress and better stress distribution compared with the conventional system. Under the conditions tested, the reversed L arrangement provided the most favourable stress dissipation behaviour.
Assuntos
Placas Ósseas , Parafusos Ósseos , Análise de Elementos Finitos , Imageamento Tridimensional/métodos , Mandíbula/cirurgia , Procedimentos Cirúrgicos Ortognáticos/instrumentação , Osteotomia Sagital do Ramo Mandibular/instrumentação , Ligas/química , Materiais Biocompatíveis/química , Fenômenos Biomecânicos , Simulação por Computador , Módulo de Elasticidade , Humanos , Avanço Mandibular/instrumentação , Miniaturização , Modelos Anatômicos , Modelos Biológicos , Poliuretanos/química , Estresse Mecânico , Titânio/químicaRESUMO
In this study, the cytotoxicity, genotoxicity and early ROS generation of 2,2-dimethyl-(3H)-3-(N-3'-nitrophenylamino)naphtho[1,2-b]furan-4,5-dione (QPhNO(2)) were investigated and compared with those of its precursor, nor-beta-lapachone (nor-beta), with the main goal of proposing a mechanism of antitumor action. The results were correlated with those obtained from electrochemical experiments held in protic (acetate buffer pH 4.5) and aprotic (DMF/TBABF(4)) media in the presence and absence of oxygen and with those from dsDNA biosensors and ssDNA in solution, which provided evidence of a positive interaction with DNA in the case of QPhNO(2). QPhNO(2) caused DNA fragmentation and mitochondrial depolarization and induced apoptosis/necrosis in HL-60 cells. Pre-treatment with N-acetyl-l-cysteine partially abolished the observed effects related to the QPhNO(2) treatment, including those involving apoptosis induction, indicating a partially redox-dependent mechanism. These findings point to the potential use of the combination of pharmacology and electrochemistry in medicinal chemistry.
Assuntos
Antineoplásicos/farmacologia , Benzofuranos/farmacologia , Naftoquinonas/farmacologia , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Dano ao DNA , Células HL-60 , Humanos , Oxirredução , Espécies Reativas de Oxigênio/metabolismoRESUMO
Although several alleles of susceptibility to Alzheimer’s disease (AD) have been studied in the last decades, few polymorphisms have been considered as risk factors for the disease. Among them, the APOE-e4 allele appears to be the major genetic risk factor for the onset of the disease. However, it is important to confirm the potential susceptibility of these genetic variants in different populations in order to establish a genetic profile for the disease in specific communities. This study analyzed the APOE polymorphisms regarding susceptibility to AD in a sample of 264 individuals (primarily Caucasians; 82 cases and 182 controls) in the population from Vitória, ES, Brazil, by PCR restriction fragment length polymorphism (PCR-RFLP) methods. The patients were selected according to clinical criteria for probable AD. Whereas the e4 allele showed statistically significant positive association with susceptibility to AD (OR = 3.01, 95 percentCI = 1.96-4.61; P < 0.0001), the e2 allele did not. The results of the e4 allele confirm the role of this polymorphism as a risk factor for AD in the sample studied as observed in other populations. Although the e3 allele has been considered neutral in several studies, our results suggest that it acts as a protective factor against AD in the population studied (OR = 0.46, 95 percentCI = 0.30-0.67; P < 0.0001). This study may provide a new insight into the role of the APOE-e3 allele in the etiology of AD and might help to estabilish a profile of risk for AD in the population from Vitória, ES.
Assuntos
Idoso de 80 Anos ou mais , Feminino , Humanos , Doença de Alzheimer/genética , /genética , Frequência do Gene , Predisposição Genética para Doença , Estudos de Casos e Controles , Genótipo , Marcadores Genéticos/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de RiscoRESUMO
True vertical maxillary deficiency is a characteristic of short face syndrome. In these patients, inferior repositioning of the maxilla (IRM) is indicated to improve facial aesthetics and function, but this procedure has been described as the most unstable. The aim of this study was to evaluate the long term, post surgical stability of IRM, fixed with four 2.0mm L-shaped miniplates, without any type of graft. A cephalometric study was performed, analysing linear measurements (anterior nasal spine, the A point, top of the incisor, top of the buccal-mesial cusp of the first molar, and posterior nasal spine on an X-Y coordinate system) traced immediately preoperatively, immediately postoperatively and at least 6 months post operatively. Eight young adult patients who underwent IRM were studied. The average results of this study were: surgical movement of 4.65 mm at I point, 5.32 mm at anterior nasal spine (ANS) point, and 4.70 mm at A point and relapses of 1.60 mm (35%), 2.23 mm (43%) and 2.10 mm (46%), respectively. It was concluded, that IRM using this type of internal rigid fixation without graft is unstable.
Assuntos
Placas Ósseas , Ossos Faciais/anormalidades , Técnicas de Fixação da Arcada Osseodentária/instrumentação , Maxila/cirurgia , Osteotomia de Le Fort/instrumentação , Adolescente , Adulto , Transplante Ósseo , Cefalometria , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteotomia de Le Fort/métodos , Recidiva , Estudos Retrospectivos , Síndrome , Dimensão Vertical , Adulto JovemRESUMO
The impact of biogeographical ancestry, self-reported 'race/color' and geographical origin on the frequency distribution of 10 CYP2C functional polymorphisms (CYP2C8*2, *3, *4, CYP2C9*2, *3, *5, *11, CYP2C19*2, *3 and *17) and their haplotypes was assessed in a representative cohort of the Brazilian population (n=1034). TaqMan assays were used for allele discrimination at each CYP2C locus investigated. Individual proportions of European, African and Amerindian biogeographical ancestry were estimated using a panel of insertion-deletion polymorphisms. Multinomial log-linear models were applied to infer the statistical association between the CYP2C alleles and haplotypes (response variables), and biogeographical ancestry, self-reported Color and geographical origin (explanatory variables). The results showed that CYP2C19*3, CYP2C9*5 and CYP2C9*11 were rare alleles (<1%), the frequency of other variants ranged from 3.4% (CYP2C8*4) to 17.3% (CYP2C19*17). Two distinct haplotype blocks were identified: block 1 consists of three single nucleotide polymorphisms (SNPs) (CYP2C19*17, CYP2C19*2 and CYP2C9*2) and block 2 of six SNPs (CYP2C9*11, CYP2C9*3, CYP2C9*5, CYP2C8*2, CYP2C8*4 and CYP2C8*3). Diplotype analysis generated 41 haplotypes, of which eight had frequencies greater than 1% and together accounted for 96.4% of the overall genetic diversity. The distribution of CYP2C8 and CYP2C9 (but not CYP2C19) alleles, and of CYP2C haplotypes was significantly associated with self-reported Color and with the individual proportions of European and African genetic ancestry, irrespective of Color self-identification. The individual odds of having alleles CYP2C8*2, CYP2C8*3, CYP2C9*2 and CYP2C9*3, and haplotypes including these alleles, varied continuously as the proportion of European ancestry increased. Collectively, these data strongly suggest that the intrinsic heterogeneity of the Brazilian population must be acknowledged in the design and interpretation of pharmacogenomic studies of the CYP2C cluster in order to avoid spurious conclusions based on improper matching of study cohorts. This conclusion extends to other polymorphic pharmacogenes among Brazilians, and most likely to other admixed populations of the Americas.
Assuntos
Hidrocarboneto de Aril Hidroxilases/genética , População Negra/genética , Sistema Enzimático do Citocromo P-450/genética , Indígenas Sul-Americanos/genética , Polimorfismo de Nucleotídeo Único , População Branca/genética , Brasil/epidemiologia , Análise por Conglomerados , Citocromo P-450 CYP2C19 , Citocromo P-450 CYP2C8 , Citocromo P-450 CYP2C9 , Frequência do Gene , Haplótipos , Humanos , Razão de ChancesRESUMO
Although several alleles of susceptibility to Alzheimer's disease (AD) have been studied in the last decades, few polymorphisms have been considered as risk factors for the disease. Among them, the APOE-e4 allele appears to be the major genetic risk factor for the onset of the disease. However, it is important to confirm the potential susceptibility of these genetic variants in different populations in order to establish a genetic profile for the disease in specific communities. This study analyzed the APOE polymorphisms regarding susceptibility to AD in a sample of 264 individuals (primarily Caucasians; 82 cases and 182 controls) in the population from Vitória, ES, Brazil, by PCR restriction fragment length polymorphism (PCR-RFLP) methods. The patients were selected according to clinical criteria for probable AD. Whereas the e4 allele showed statistically significant positive association with susceptibility to AD (OR = 3.01, 95%CI = 1.96-4.61; P < 0.0001), the e2 allele did not. The results of the e4 allele confirm the role of this polymorphism as a risk factor for AD in the sample studied as observed in other populations. Although the e3 allele has been considered neutral in several studies, our results suggest that it acts as a protective factor against AD in the population studied (OR = 0.46, 95%CI = 0.30-0.67; P < 0.0001). This study may provide a new insight into the role of the APOE-e3 allele in the etiology of AD and might help to establish a profile of risk for AD in the population from Vitória, ES.
Assuntos
Doença de Alzheimer/genética , Apolipoproteína E3/genética , Frequência do Gene , Predisposição Genética para Doença , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Feminino , Marcadores Genéticos/genética , Genótipo , Humanos , Masculino , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de RiscoRESUMO
OBJECTIVES: The purpose of this study was to cephalometrically evaluate the pharyngeal airway space and frontal and sphenoid sinus changes after maxillomandibular advancement counterclockwise rotation for class II anterior open bite malocclusion. METHODS: The study included 49 patients (98 lateral teleradiographs; 36 females and 13 males) who were analysed in the pre-operative (1 week before surgery) and post-operative (6 months after surgery) periods. In each lateral teleradiography, the dimensions of the inferior and superior pharyngeal airway space, TB-PhW1 [the point between the posterior aspect of the tongue to the dorsal pharyngeal wall (oropharynx) (TB) and the point on the dorsal pharyngeal wall closest to TB (PhW1)] and UP-PhW2 [and the point between the posterior aspect of the soft palate to the dorsal pharyngeal wall (nasopharynx) (UP) (PhW2)] measurements were evaluated, as well as the dimensions of the frontal and sphenoid sinuses. The differences between the two operative times were evaluated by Student's t-test. RESULTS: All measurements showed excellent reproducibility for the intraclass correlation coefficient (ICC > 0.9; p < 0.0001). There was an increase in the measurements TB-PhW1 and UP-PhW2 and a decrease in the dimensions of the frontal and sphenoid sinuses after orthognathic surgery. CONCLUSIONS: The morphology of the superior and inferior pharyngeal airway space and frontal and sphenoid sinuses changes after 6 months of maxillomandibular advancement counterclockwise rotation for class II anterior open bite malocclusion.
Assuntos
Seio Frontal/anatomia & histologia , Mordida Aberta/cirurgia , Faringe/anatomia & histologia , Seio Esfenoidal/anatomia & histologia , Adulto , Cefalometria , Feminino , Humanos , Masculino , Avanço Mandibular , Maxila/cirurgia , Obstrução Nasal/cirurgia , Nariz/anatomia & histologia , Ventilação Pulmonar , Adulto JovemRESUMO
El objetivo de este estudio fue realizar un análisis retrospectivo de la etiología, incidencia, uso de dispositivos de seguridad, tipo y severidad de las fracturas, tratamiento y complicaciones de los traumatismos del tercio medio facial en una población pediátrica e adolescente del Estado de São Paulo. La muestra fue seleccionada a partir del análisis de historias clínicas de 2986 pacientes víctimas de traumatismos faciales atendidos entre 1999 y 2008 por el Área de Cirugía Buco-Maxilo-Facial de la Facultad de Odontología de Piracicaba, Universidad Estatal de Campinas, Estado de São Paulo-Brasil. Durante este periodo, 757 pacientes menores de 18 años fueron víctimas de traumatismos maxilofaciales. De éstos, 138 pacientes presentaron 148 fracturas nasales y de tercio medio. Los adolescentes fueron el grupo etario más afectado, mientras que el género masculino fue el de mayor prevalencia. Los accidentes ciclísticos se constituyeron en la principal etiología (21.7%). El tratamiento conservador fue utilizado en la gran mayoría de los casos (118 pacientes, 85.5%). Veinte pacientes (14.5%) recibieron tratamiento quirúrgico y solo un caso de complicación pos-operatoria fue reportado. La incidencia de traumatismos y fracturas del tercio medio fue alta en la región de estudio. Los accidentes ciclísticos y las agresiones físicas fueron los principales factores etiológicos, siendo el género masculino predominante en todos los grupos etarios evaluados. Las fracturas nasales y del complejo zigomático orbitario fueron las más prevalentes, mientras que el tratamiento conservador fue instituido en la gran mayoría de los casos
This study showed a retrospective analysis of the etiology, incidence, use of safety devices, type and severity of fractures, treatment and complications of midface trauma in a pediatric and adolescent population of São Paulo - Brazil. We analyzed 2986 medical records of facial trauma patients treated by the Division of Oral and Maxillofacial Surgery Piracicaba Dental School, State University of Campinas, São Paulo - Brazil between 1999 and 2008. During this period, 757 patients under 18 were identified and 138 had 148 nasal and midface fractures. The adolescents were the most affected age group, male gender being the most prevalent. The most common injury causes were bicycle accident (21.74%) and violence (19.6%). Conservative treatment was used in the majority of cases (118 patients, 85.5%). Twenty patients (14.5%) received surgical treatment and only one case of postoperative complication was reported. The incidence of trauma and middle third fractures in pediatric and adolescents patients was high in the study area, being the main etiological factors the bicycle accidents and violence. The male were most affected in all age groups tested. Nasal fractures and zygomatic complex were most prevalent, while conservative treatment was instituted in most cases