RESUMO
At present, more than 75% of routine nuclear medicine diagnostic procedures use technetium-99m (99mTc). The binding between 99mTc and the drug to obtain the radiopharmaceutical needs a reducing agent, with stannous chloride (SnCl2) being one of the most used. There are controversies about the cytotoxic, genotoxic and mutagenic effects of SnCl2 in the literature. Thus, the approaches below were used to better understand the biological effects of this salt and its association in nuclear medicine kits [methylenediphosphonate (MDP) bone scintigraphy and diethylenetriaminepentaacetic acid (DTPA) kidney and brain scintigraphy]: (i) bacterial inactivation experiments; (ii) agarose gel electrophoresis of supercoiled and linear plasmid DNA and (iii) bacterial transformation assay. The Escherichia coli strains used here were AB1157 (wild type) and BW9091 (xthA mutant). Data obtained showed that both MDP and SnCl2 presented a high toxicity, but this was not observed when they were assayed together in the kit, thereby displaying a mutual protect effect. DTPA salt showed a moderate toxicity, and once more, the DTPA kit provided protection, compared to the SnCl2 effect alone. The results suggest a possible complex formation, either MDP-SnCl2 or DTPA-SnCl2, originating an atoxic compound. On the other hand, SnCl2-induced cell inactivation and the decrease in bacterial transformation generated by DTPA found in XthA mutant strain suggest that the lack of this enzyme could be responsible for the effects observed, being necessary to induce DNA damage repair.
Assuntos
DNA Bacteriano/efeitos dos fármacos , Escherichia coli/citologia , Escherichia coli/efeitos dos fármacos , Medicina Nuclear/instrumentação , Kit de Reagentes para Diagnóstico , Compostos de Estanho/administração & dosagem , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Escherichia coli/genética , Testes de Mutagenicidade , MutaçãoRESUMO
Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS); therefore, studies have been carried out in order to better understand its damaging action in biological systems. In this work, calf thymus DNA, triphosphate nucleotides and isolated bases were incubated with SnCl2 and the results were analyzed through UV spectrophotometry. The presence of stannous ions altered the absorption spectra of all three isolates. The amount of stannous ions associated to DNA was measured by atomic absorption spectrophotometry. Data showed that more than 40% of the initial SnCl2 concentration was present in the samples. Our results are in accordance with the damaging potential of this salt and present evidence that stannous ions can complex with DNA, inducing ROS in its vicinity, which may be responsible for the observed lesions.
Assuntos
Dano ao DNA/efeitos dos fármacos , DNA/química , DNA/efeitos dos fármacos , Nucleotídeos/metabolismo , Compostos de Estanho/farmacologia , Animais , Bovinos , DNA/metabolismo , Espectrofotometria Atômica , Espectrofotometria Ultravioleta , Raios UltravioletaRESUMO
Aloe vera is a tropical plant, known in Brazil as babosa and several reputable suppliers produce a stabilized aloe gel for topic use. Since people use Aloe vera topically, they could be exposed to solar ultraviolet light in addition and it might cause a cross damage effect between these agents. The aim of this work was to investigate the biological effects of Aloe vera pulp extract, associated or not to UVA radiation, on Escherichia coli-deficient repair mutants and plasmid DNA, in order to test its genotoxic potential. Data obtained from analysis of survival fractions, bacterial transformation and agarose gel electrophoresis suggest that Aloe vera has genotoxic properties, but it seems not to be able to damage the cell membrane.