RESUMO
Multilocus genotyping of microbial pathogens has revealed a range of population structures, with some bacteria showing extensive recombination and others showing almost complete clonality. The population structure of the protozoan parasite Plasmodium falciparum has been harder to evaluate, since most studies have used a limited number of antigen-encoding loci that are known to be under strong selection. We describe length variation at 12 microsatellite loci in 465 infections collected from 9 locations worldwide. These data reveal dramatic differences in parasite population structure in different locations. Strong linkage disequilibrium (LD) was observed in six of nine populations. Significant LD occurred in all locations with prevalence <1% and in only two of five of the populations from regions with higher transmission intensities. Where present, LD results largely from the presence of identical multilocus genotypes within populations, suggesting high levels of self-fertilization in populations with low levels of transmission. We also observed dramatic variation in diversity and geographical differentiation in different regions. Mean heterozygosities in South American countries (0.3-0.4) were less than half those observed in African locations (0. 76-0.8), with intermediate heterozygosities in the Southeast Asia/Pacific samples (0.51-0.65). Furthermore, variation was distributed among locations in South America (F:(ST) = 0.364) and within locations in Africa (F:(ST) = 0.007). The intraspecific patterns of diversity and genetic differentiation observed in P. falciparum are strikingly similar to those seen in interspecific comparisons of plants and animals with differing levels of outcrossing, suggesting that similar processes may be involved. The differences observed may also reflect the recent colonization of non-African populations from an African source, and the relative influences of epidemiology and population history are difficult to disentangle. These data reveal a range of population structures within a single pathogen species and suggest intimate links between patterns of epidemiology and genetic structure in this organism.
Assuntos
Evolução Molecular , Frequência do Gene , Malária Falciparum/epidemiologia , Repetições de Microssatélites , Plasmodium falciparum/genética , África/epidemiologia , Animais , Evolução Biológica , Variação Genética , Genótipo , Geografia , Humanos , Desequilíbrio de Ligação , Papua Nova Guiné/epidemiologia , Plasmodium falciparum/classificação , Probabilidade , América do SulRESUMO
Allelic diversity at the Plasmodium vivax merozoite surface protein-3alpha (PvMsp-3alpha) locus was investigated using a combined polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) protocol. Symptomatic patient isolates from global geographic origins showed a high level of polymorphism at the nucleotide level. These samples were used to validate the sensitivity, specificity, and reproducibility of the PCR/RFLP method. It was then used to investigate PvMsp3alpha diversity in field samples from children living in a single village in a malaria-endemic region of Papua New Guinea, with the aim of assessing the usefulness of this locus as an epidemiologic marker of P. vivax infections. Eleven PvMsp-3alpha alleles were distinguishable in 16 samples with single infections, revealing extensive parasite polymorphism within this restricted area. Multiple infections were easily detected and accounted for 5 (23%) of 22 positive samples. Pairs of samples from individual children provided preliminary evidence for high turnover of P. vivax populations.
Assuntos
Antígenos de Protozoários/genética , Variação Genética/genética , Malária Vivax/parasitologia , Plasmodium vivax/genética , Proteínas de Protozoários/genética , Adolescente , Animais , Antígenos de Protozoários/análise , Antígenos de Superfície/análise , Antígenos de Superfície/genética , Biomarcadores/sangue , Brasil/epidemiologia , Criança , Pré-Escolar , Primers do DNA/química , DNA de Protozoário/química , DNA de Protozoário/isolamento & purificação , El Salvador/epidemiologia , Eletroforese em Gel de Ágar , Humanos , Índia/epidemiologia , Malária Vivax/epidemiologia , Papua Nova Guiné/epidemiologia , Parasitemia/epidemiologia , Parasitemia/parasitologia , Plasmodium vivax/isolamento & purificação , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Proteínas de Protozoários/análise , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Sri Lanka/epidemiologia , Sudão/epidemiologiaRESUMO
Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting that PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.
Assuntos
Proteínas Sanguíneas/genética , Deleção Cromossômica , Plasmodium falciparum/genética , Proteínas de Protozoários , Animais , Proteínas Sanguíneas/fisiologia , Adesão Celular/genética , Cromossomos Artificiais de Levedura , Eletroforese em Gel de Campo Pulsado , Genes de Protozoários , Parasitologia/métodos , Peptídeos/genética , Peptídeos/fisiologia , Plasmodium falciparum/fisiologia , Reprodução , Seleção Genética , Células Tumorais CultivadasRESUMO
Many lines of Plasmodium falciparum undrgo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail