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J Am Heart Assoc ; 5(4): e003170, 2016 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-27091183

RESUMO

BACKGROUND: Unlike arteries, in which regionally distinct hemodynamics are associated with phenotypic heterogeneity, the relationships between endocardial endothelial cell phenotype and intraventricular flow remain largely unexplored. We investigated regional differences in left ventricular wall shear stress and their association with endocardial endothelial cell gene expression. METHODS AND RESULTS: Local wall shear stress was calculated from 4-dimensional flow magnetic resonance imaging in 3 distinct regions of human (n=8) and pig (n=5) left ventricle: base, adjacent to the outflow tract; midventricle; and apex. In both species, wall shear stress values were significantly lower in the apex and midventricle relative to the base; oscillatory shear index was elevated in the apex. RNA sequencing of the endocardial endothelial cell transcriptome in pig left ventricle (n=8) at a false discovery rate ≤10% identified 1051 genes differentially expressed between the base and the apex and 327 between the base and the midventricle; no differentially expressed genes were detected at this false discovery rate between the apex and the midventricle. Enrichment analyses identified apical upregulation of genes associated with translation initiation including mammalian target of rapamycin, and eukaryotic initiation factor 2 signaling. Genes of mitochondrial dysfunction and oxidative phosphorylation were also consistently upregulated in the left ventricular apex, as were tissue factor pathway inhibitor (mean 50-fold) and prostacyclin synthase (5-fold)-genes prominently associated with antithrombotic protection. CONCLUSIONS: We report the first spatiotemporal measurements of wall shear stress within the left ventricle and linked regional hemodynamics to heterogeneity in ventricular endothelial gene expression, most notably to translation initiation and anticoagulation properties in the left ventricular apex, in which oscillatory shear index is increased and wall shear stress is decreased.


Assuntos
Endocárdio/metabolismo , Ventrículos do Coração/metabolismo , RNA/genética , Resistência ao Cisalhamento/fisiologia , Animais , Técnicas de Imagem Cardíaca , Endocárdio/diagnóstico por imagem , Endocárdio/fisiologia , Feminino , Perfilação da Expressão Gênica , Biblioteca Genômica , Ventrículos do Coração/diagnóstico por imagem , Hemodinâmica , Humanos , Imageamento por Ressonância Magnética , Masculino , Suínos , Função Ventricular Esquerda/fisiologia , Adulto Jovem
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