RESUMO

Nitrogen (N) and silicon (Si) are mineral elements that have shown a reduction in the damage caused by tan spot (Pyrenophora tritici-repentis (Ptr)) in wheat. However, the effects of these elements were studied separately, and the N and Si interaction effect on wheat resistance to tan spot remains elusive. Histocytological and biochemical defense responses against Ptr in wheat leaves treated with Si (+Si) at low (LN) and high N (HN) inputs were investigated. Soil amendment with Si reduced the tan spot severity in 18% due to the increase in the leaf Si concentration (around 30%), but it was affected by the N level used. The superoxide dismutase (SOD) activity was higher in +Si plants and inoculated with Ptr, leading to early and higher H2O2 and callose accumulation in wheat leaf. Interestedly, phenylalanine ammonia-lyase (PAL) activity was induced by the Si supplying, being negatively affected by the HN rate. Meanwhile, catalase (CAT), and peroxidase (POX) activities showed differential response patterns according to the Si and N rates used. Tan spot severity was reduced by both elements, but their interaction does not evidence synergic effects in this disease's control. Wheat plants from -Si and HN and +Si and LN treatments recorded lower tan spot severity.

RESUMO

The rice water weevil, Oryzophagus oryzae (Coleoptera: Curculionidae), is an economically important pest of flooded rice paddies throughout South America, and species with similar life histories are present in many rice-producing regions globally (collectively referred to here as RWWs). Plant resistance is a key strategy for management of RWWs; however, the mechanisms responsible for rice resistance to RWWs are poorly understood. We investigated morphoanatomical and biochemical plant traits potentially involved in rice resistance to O. oryzae. Resistance-associated traits were characterized in two cultivars, 'Dawn' (resistant) and 'BRS Pampa CL' ('Pamp' = susceptible), which were selected from among six cultivars on 2-year field screenings. Anatomical and morphological traits of leaf tissues from 'Pamp' and 'Dawn' were similar, which perhaps explains the lack of antixenosis during host plant selection. However, significant antibiosis effects were found. The activities of antioxidant enzymes involved in plant defense, as well the content of hydroxycinnamic and hydroxybenzoic acids derivatives and lignin, were higher in roots of 'Dawn' than in 'Pamp', over the period of larval infestation in the field. Additionally, 'Dawn' exhibited a root sclerenchyma arranged in three layers of lignified cells, which differed from the arrangement of cells in 'Pamp', regardless of larval infestation. Our results provide the first evidence for specific resistance-related traits associated with mortality and malnutrition of RWWs in rice.

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RESUMO

Fusarium head blight (FHB), caused mainly by Fusarium graminearum, is best controlled with demethylation inhibitor (DMI) fungicides during flowering. However, the use of premixes of DMI and quinone outside inhibitor (QoI) fungicides to control FHB has increased in Brazil. Data on FHB severity and wheat yields measured in field experiments conducted in Brazil were gathered from both peer- and nonpeer-reviewed sources published from 2000 to 2018. After selection criteria were applied, 73 field trials from 35 bibliographic sources were identified, among which 50% of the data were obtained from cooperative network trials conducted after 2011. To be included in the analysis, DMI plus QoI premixes or tebuconazole were tested in at least 14 trials and 3 years. Four premixes met the criteria. Estimates of percent control (and respective 95% confidence intervals) by a network model fitted to the log of the treatment means ranged from 44.1% (pyraclostrobin plus metconazole applied once; 32.4 to 53.7) to 64.3% (pyraclostrobin plus metconazole; 58.4 to 69.3); the latter did not differ from tebuconazole (59.9%; 53.6 to 65.3). Yield response was statistically similar for pyraclostrobin plus metconazole (532.1 kg/ha; 441 to 623) and trifloxystrobin plus prothioconazole (494.9 kg/ha; 385 to 551), and both differed statistically from a group composed of tebuconazole (448.2 kg/ha; 342 to 554), trifloxystrobin plus tebuconazole (468.2 kg/ha; 385 to 551), azoxystrobin plus tebuconazole (462.4 kg/ha; 366 to 558), and pyraclostrobin plus metconazole applied once (413.7 kg/ha; 308 to 518). The two categories of FHB index (7% cutoff) and yield (3,000 kg/ha cutoff), both in the nontreated check, did not explain the heterogeneity in the estimates. Considering only the fungicide effects on yield, two sequential sprays of tebuconazole or one spray of pyraclostrobin plus metconazole as management choices are likely more profitable than DMI plus QoI premixes sprayed twice during flowering.

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RESUMO

The objective of the research was to evaluate the residual effect of the seeds treatment with fungicide and silicate fertilizer in the control of brown spot in the vegetative stage of the barley plant. The experiment was a two-way factorial with four seed treatments (ST): control (distilled water), fungicide (triadimenol), silicate fertilizer and the fungicide + silicate fertilizer and three periods of 30, 45 and 60 days after emergence (DAE). Barley plants were inoculated with a spore suspension of Bipolaris sorokiniana. The disease severity assessment over time was used to calculate the area under the brown spot progress curve (AUCPBS). At 30 DAE, fungicide treatment was the most efficient with reduction of 64% in the AUCPBS, followed by silicate fertilizer with 56%. The mixture also reduced AUCPBS, but with lower efficiency than isolated products.(AU)

O objetivo da pesquisa foi avaliar o efeito residual do tratamento das sementes de cevada com fungicida e fertilizante silicatado no controle da mancha marrom no estágio vegetativo da planta. O delineamento experimental utilizado foi inteiramente casualizado em esquema bifatorial 4 × 3, constituído por quatro tratamento de sementes (TS): testemunha (água destilada), fungicida (triadimenol), fertilizante silicatado e a mistura fungicida + fertilizante silicatado e plantas com 30, 45 e 60 dias após a emergência (DAE). Para avaliar o efeito residual (TS), as plantas foram inoculadas com uma suspensão de esporos de Bipolaris sorokiniana. A avaliação da severidade ao longo do tempo foi utilizada para calcular a área abaixo da curva de progresso da mancha marrom (AACPMM). Aos 30 DAE, o tratamento com fungicida foi o mais eficiente com a redução de 64% na AACPMM, seguido do fertilizante silicatado com 56%. A mistura também reduziu a AACPMM, mas com eficiência inferior aos produtos isolados.(AU)

Assuntos

RESUMO

O objetivo do trabalho foi avaliar o efeito dos produtos acibenzolar-s-metil, fosfito de potássio, silicato de potássio e trifloxistrobina + protioconazole no controle curativo do oídio (Microsphaera diffusa) na soja. O delineamento experimental foi inteiramente casualizado, em esquema unifatorial, contendo cinco tratamentos com quatro repetições. A aplicação dos produtos foi realizada no estádio fenológico V4, no qual as plantas possuíam 5% de severidade da doença. Aos 7, 14 e 21 dias após aplicação dos tratamentos (DAA), foi avaliada a incidência e severidade da doença. O trifloxistrobina + protioconazole reduziu a incidência em 30% e a severidade em 80%, quando comparado ao controle. Também foi avaliado o efeito inibitório dos produtos na produção de conídios in vivo e na sua germinação in vitro. O trifloxistrobina + protioconazole proporcionou a maior redução na produção de conídios e na inibição da germinação. O acibenzolar-s-metil, fosfito de potássio e silicato de potássio também reduziram a germinação de conídios, mas não afetaram a produção. Com base nisso, conclui-se que, o fungicida trifloxistrobina + protioconazole reduziu a produção de conídios e inibiu sua germinação, apresentando efeito curativo e protetor contra oídio em soja e que os indutores de resistência testados, interferem na germinação do patógeno, mas sem efeito significativo na doença quando aplicados...(AU)

The objective of this work was to assess the effect of acibenzolar-s-methyl, potassium phosphite, potassium silicate, and trifloxystrobin + prothioconazole products on curative control of powdery mildew (Microsphaera diffusa) in soybean. The experimental design was completely randomized, in a single factor scheme, containing five treatments with four replications. The products were applied on leaves at the V4 phenological stage, with disease severity of 5%. At 7, 14, and 21 days after the application of the products (DAA), the incidence and severity of the disease were assessed. The trifloxystrobin + prothioconazole reduced by 30% the disease incidence and 80% disease severity compared to the control. The inhibitory effect of the products on conidia production in vivo and its germination in vitro were also assessed. Trifloxystrobin + prothioconazole was more effective in the reduction of both conidia production and germination. Acibenzolar-s-methyl, potassium phosphite, and potassium silicate reduced conidia germination, but neither affected the disease severity nor the conidia production. Based on this, it can be concluded that the fungicide trifloxystrobin + prothioconazole reduced the production of conidia and inhibited its germination, showing curative and protective effect against soybean powdery mildew and that the resistance inducers tested interfered with pathogen...(AU)

Assuntos

RESUMO

O objetivo do trabalho foi avaliar o efeito dos produtos acibenzolar-s-metil, fosfito de potássio, silicato de potássio e trifloxistrobina + protioconazole no controle curativo do oídio (Microsphaera diffusa) na soja. O delineamento experimental foi inteiramente casualizado, em esquema unifatorial, contendo cinco tratamentos com quatro repetições. A aplicação dos produtos foi realizada no estádio fenológico V4, no qual as plantas possuíam 5% de severidade da doença. Aos 7, 14 e 21 dias após aplicação dos tratamentos (DAA), foi avaliada a incidência e severidade da doença. O trifloxistrobina + protioconazole reduziu a incidência em 30% e a severidade em 80%, quando comparado ao controle. Também foi avaliado o efeito inibitório dos produtos na produção de conídios in vivo e na sua germinação in vitro. O trifloxistrobina + protioconazole proporcionou a maior redução na produção de conídios e na inibição da germinação. O acibenzolar-s-metil, fosfito de potássio e silicato de potássio também reduziram a germinação de conídios, mas não afetaram a produção. Com base nisso, conclui-se que, o fungicida trifloxistrobina + protioconazole reduziu a produção de conídios e inibiu sua germinação, apresentando efeito curativo e protetor contra oídio em soja e que os indutores de resistência testados, interferem na germinação do patógeno, mas sem efeito significativo na doença quando aplicados...

The objective of this work was to assess the effect of acibenzolar-s-methyl, potassium phosphite, potassium silicate, and trifloxystrobin + prothioconazole products on curative control of powdery mildew (Microsphaera diffusa) in soybean. The experimental design was completely randomized, in a single factor scheme, containing five treatments with four replications. The products were applied on leaves at the V4 phenological stage, with disease severity of 5%. At 7, 14, and 21 days after the application of the products (DAA), the incidence and severity of the disease were assessed. The trifloxystrobin + prothioconazole reduced by 30% the disease incidence and 80% disease severity compared to the control. The inhibitory effect of the products on conidia production in vivo and its germination in vitro were also assessed. Trifloxystrobin + prothioconazole was more effective in the reduction of both conidia production and germination. Acibenzolar-s-methyl, potassium phosphite, and potassium silicate reduced conidia germination, but neither affected the disease severity nor the conidia production. Based on this, it can be concluded that the fungicide trifloxystrobin + prothioconazole reduced the production of conidia and inhibited its germination, showing curative and protective effect against soybean powdery mildew and that the resistance inducers tested interfered with pathogen...

Assuntos

RESUMO

The objective of the research was to evaluate the residual effect of the seeds treatment with fungicide and silicate fertilizer in the control of brown spot in the vegetative stage of the barley plant. The experiment was a two-way factorial with four seed treatments (ST): control (distilled water), fungicide (triadimenol), silicate fertilizer and the fungicide + silicate fertilizer and three periods of 30, 45 and 60 days after emergence (DAE). Barley plants were inoculated with a spore suspension of Bipolaris sorokiniana. The disease severity assessment over time was used to calculate the area under the brown spot progress curve (AUCPBS). At 30 DAE, fungicide treatment was the most efficient with reduction of 64% in the AUCPBS, followed by silicate fertilizer with 56%. The mixture also reduced AUCPBS, but with lower efficiency than isolated products.

O objetivo da pesquisa foi avaliar o efeito residual do tratamento das sementes de cevada com fungicida e fertilizante silicatado no controle da mancha marrom no estágio vegetativo da planta. O delineamento experimental utilizado foi inteiramente casualizado em esquema bifatorial 4 × 3, constituído por quatro tratamento de sementes (TS): testemunha (água destilada), fungicida (triadimenol), fertilizante silicatado e a mistura fungicida + fertilizante silicatado e plantas com 30, 45 e 60 dias após a emergência (DAE). Para avaliar o efeito residual (TS), as plantas foram inoculadas com uma suspensão de esporos de Bipolaris sorokiniana. A avaliação da severidade ao longo do tempo foi utilizada para calcular a área abaixo da curva de progresso da mancha marrom (AACPMM). Aos 30 DAE, o tratamento com fungicida foi o mais eficiente com a redução de 64% na AACPMM, seguido do fertilizante silicatado com 56%. A mistura também reduziu a AACPMM, mas com eficiência inferior aos produtos isolados.

Assuntos

RESUMO

Wheat (Triticum aestivum) is an annual crop, cultivated in the winter and spring and susceptible to several pathogens, especially fungi, which are managed with fungicides. It is also one of the most consumed cereals, and can be contaminated by mycotoxins and fungicides. The objective of this study was to validate an analytical method by LC-MS for simultaneous determination of mycotoxins and fungicide residues in wheat grains susceptible to fusarium head blight treated with fungicides, and to evaluate the relationship between fungicide application and mycotoxin production. All parameters of the validated analytical method were within AOAC and ANVISA limits. Deoxynivalenol was the prevalent mycotoxin in wheat grain and epoxiconazole was the fungicide residue found in the highest concentration. All fungicidal treatments induced an increase in AFB2 production when compared to the control (without application). AFB1 and deoxynivalenol, on the contrary, were reduced in all fungicide treatments compared to the control.

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RESUMO

Our study surveyed Meloidogyne spp. from tobacco cultivars in Brazil carrying the Rk gene, which confers resistance to Meloidogyne incognita races 1 and 3. Identification of Meloidogyne populations from 39 tobacco fields was based on the analysis of esterase phenotypes, perineal patterns, and 18S-internal transcribed spacer (ITS)1-5.8S ribosomal RNA sequences. Biometric characterization and differential host tests of isolates were determined as well. We detected M. incognita, M. javanica, M. enterolobii, M. arenaria, and M. inornata in 18 (46.2%), 16 (41%), 10 (25.6%), 2 (5.1%), and 1 (2.6%) samples, respectively. Mixtures of species were found in 25.6% of the samples. This is the first report of M. inornata parasitizing tobacco in Brazil's southern region. Two morphological and biochemically unusual populations had host ranges and ITS1 sequences nearly identical to M. enterolobii and M. incognita, respectively. Pathogenic and biometric intraspecific variations were observed. Based on our results and considering the limited efficiency of the Rk gene, it is indispensable not only to search for resistance sources to M. incognita virulent strains but also to consider resistance to M. enterolobii, M. inornata, M. arenaria, and M. javanica in tobacco breeding programs. Changes in integrated management procedures should be considered to avoid increased crop damage in the future.