RESUMO
AIM: The aim of this study was to investigate the effects of acute and chronic systemic stress response on orthodontically induced root resorption. METHODS: Male Wistar rats were restrained during 1 hour a day by stress models of short (3 days) and long duration (40 days), while control group was not submitted to restraint (N.=10/group). The upper left first molars of all rats were moved mesially by a fixed orthodontic appliance exerting 50 g force upon insertion during the last 14 days of the experiment. Then, animals were killed for blood collection and mensuration of plasmatic corticosterone by radioimmunoassay; the tissues around mesial root of the first molar were processed for histological and histochemical techniques with tartrate-resistant acid phosphatase. The degree of root resorption and the number of odontoclasts were evaluated, being the contralateral side of each animal serving as its control (split-mouth design). RESULTS: The results revealed that the plasmatic levels of corticosterone were significantly higher in both the stressed groups than in the control one. There were no significant differences in the degree of root resorption and in the number of odontoclasts on the root between the 3 groups studied. CONCLUSIONS: These results indicate that systemic stress alone can not be considered a risk factor for root resorption induced by orthodontic tooth movement.
Assuntos
Aparelhos Ortodônticos/efeitos adversos , Reabsorção da Raiz/etiologia , Estresse Psicológico/fisiopatologia , Técnicas de Movimentação Dentária/efeitos adversos , Fosfatase Ácida/análise , Animais , Biomarcadores , Contagem de Células , Corticosterona/sangue , Isoenzimas/análise , Masculino , Dente Molar/enzimologia , Dente Molar/patologia , Desenho de Aparelho Ortodôntico , Osteoclastos/patologia , Ratos , Ratos Wistar , Restrição Física , Reabsorção da Raiz/sangue , Reabsorção da Raiz/patologia , Estresse Psicológico/sangue , Estresse Psicológico/etiologia , Fosfatase Ácida Resistente a Tartarato , Fatores de Tempo , Técnicas de Movimentação Dentária/métodosRESUMO
AIM: Causes of temporomandibular disorders (TMDs) are multifactorial, involving both physical and psychological factors. The aim of this study was to evaluate the salivary cortisol levels in young adults with TMDs. METHODS: The Research Diagnostic Criteria (RDC)/TMDs were used to establish the diagnosis of TMDs regarding the physical (axis I) and psychological factors (depression and somatization; axis II). Young adults of both genders composed the TMDs males (21.7+/-2.21 years, n=10) and females (22.7+/-1.95 years n=10) groups, and 20 healthy matched controls composed the control male (22.5+/-1.96 years n=10) and female (22.4+/-2.17years n=10) groups. Saliva samples were collected immediately on awakening and at bedtime. The cortisol levels were measured by enzyme immunoassay. RESULTS: The female group with TMDs showed a significantly higher cortisol level (2.890+/-0.411 mg/dL) than the corresponding control group in the morning evaluation (2.470+/- 0.486 mg/dL). The depression scores were significantly higher for the female group with TMDs (1.3+/-0.18) in comparison with the control female (0.72+/-0.18) and male groups (0.51+/-0.13). The groups with TMDs showed significantly higher somatization scores (0.69+/-0.16 for males and 1.38+/-0.23 for females) in comparison with the controls (0.23+/-0.10 for males and 0.78+/-0.15 for females), these also being significantly higher for the females than males. CONCLUSION: It was concluded that cortisol levels are higher in TMDs in females, who had elevated depression and somatization scores. The results infer that the evaluation of salivary cortisol could be a promising tool in the diagnosis of TMDs associated with psychological factors.