RESUMO
Cystic fibrosis is characterized by abnormal mucous secretions in the lungs that favor the proliferation of colonizing bacteria, with Pseudomonas aeruginosa and Staphylococcus aureus being the most isolated, however, other less known species could also have an impact on the health of the patient. Here we demonstrate the isolation and antibiotic resistance profiles of Inquilinus limosus, a rarely reported multidrug resistant bacterium, and compare them to a co-infectant strain of Pseudomonas aeruginosa. Likewise, we found that co-infection with both bacteria promotes increased formation of neutrophil extracellular traps, which can have an impact on the disease severity and make treatment difficult.
Assuntos
Coinfecção , Fibrose Cística , Armadilhas Extracelulares , Infecções por Pseudomonas , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Coinfecção/tratamento farmacológico , Fibrose Cística/complicações , Humanos , México , Neutrófilos , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa , RhodospirillaceaeRESUMO
Resumen Introducción: El panorama de la salud y nutrición de la población mexicana se enmarca en la conjunción de desnutrición crónica infantil, sobre todo en el medio rural e indígena, y el incremento significativo del sobrepeso y obesidad. Esta información proveniente de encuestas arroja resultados generalizados, sin considerar las particularidades del contexto biosociocultural en que viven comunidades indígenas, codeterminante de la condición nutricional de éstas. Objetivo: Estudiar el estatus nutricional de un grupo de escolares de cultura otomí residentes en una comunidad de México en situación de marginación, considerando el contexto biosociocultural en el que viven. Métodos: Estudio prospectivo transversal realizado en una localidad del Estado de México. Muestra por conveniencia constituida por 214 menores, entre 6 y 12 años de edad. Se emplearon técnicas antropométricas internacionalmente aceptadas para recabar la estatura y el peso, y se calculó el IMC. El estatus de nutrición se estimó con base en el peso, la estatura y el IMC para la edad y se compararon con los referentes de la OMS. Resultados: La prevalencia de estatura baja para la edad y la desnutrición es menor, no así el sobrepeso/obesidad que afecta a un tercio de los menores. Solo para el IMC por edad y sexo hubo diferencias en ciertos grupos de edad. Las puntuaciones z de los tres indicadores son semejantes entre niños y niñas. Discusión y Conclusiones: En comparación con los parámetros nacional y estatal, los menores estudiados muestran prevalencia menor de sobrepeso y obesidad asociado a su contexto sociocultural.
Abstract Introduction: The health and nutrition outlook of the Mexican population may be framed in terms of chronic infant malnutrition, largely in the rural and indigenous sectors, and a significant increase in overweight and obesity. This general information is obtained from surveys without taking into account the biosociocultural context that is a codeterminant of the nutritional condition of many small indigenous communities. Objective: To study the nutritional status of Otomí school children living in a marginalized Mexican community from the perspective of the biosociocultural context in which they live. Methods: This is a prospective and transversal study carried out in a location of the state of México, México. The convenience sample was constituted by 214 children between 6 and 12 years old. Internationally accepted anthropometric techniques were used to collect data on the height and weight of these children. BMIs were calculated. The status of nutrition was estimated based on the expected weight, height, and BMI for each age. These data were compared with the corresponding WHO references. Results: The prevalence of low height and malnutrition related to the ages of these children was found to be low, but one in three of these children was found to be overweight or obese. There were some age groups differences regarding the BMI. The z scores of the three indicators are similar between boys and girls. Discussion and Conclusions: In comparison to the national and state parameters, the children studied showed a low prevalence of overweight and obesity associated with their sociocultural context.
Resumo Introdução: O panorama da saúde e nutrição da população mexicana se enquadra na conjunção de desnutrição infantil crónica, sobretudo no médio rural e indígena, e o incremento significativo do sobrepeso e obesidade. Esta informação vinda de enquetes gera resultados generalizados, sem considerar as particularidades do contexto biosociocultural em que vivem comunidades indígenas, co-determinante da condição nutricional destas. Objetivo: Estudar o estado nutricional de um grupo de escolares de cultura Otomí residentes em uma comunidade do México em situação de marginação, considerando o contexto biosociocultural em que vivem. Métodos: Estudo prospectivo transversal realizado em uma localidade do Estado do México. Amostra de conveniência constituída por 214 menores entre 6 e 12 anos de idade. Empregaram-se técnicas antropométricas internacionalmente aceitas para coletar a altura e o peso, e foi calculado o IMC. O estado de nutrição foi estimado com base no peso, a altura e o IMC para a idade e foram comparados com os referentes da OMS. Resultados: A prevalência da baixa altura para a idade e a desnutrição é menor, no entanto o sobrepeso/obesidade afeta a um terço dos meninos. Apenas para o IMC por grupos de idade e sexo houve diferenças em determinadas faixas etárias. As pontuações z dos três indicadores são semelhantes entre meninos e meninas. Discussão e Conclusões: Em comparação com os parâmetros nacionais e estaduais, os meninos estudados mostram menor prevalência de sobrepeso e obesidade associado ao seu contexto sociocultural.
RESUMO
PURPOSE: The aim of this study was to assess the feasibility and treatment outcome of intensity modulated radiation therapy with simultaneous integrated boost (SIB-IMRT) in locally advanced non-small cell lung cancer (NSCLC) patients. MATERIALS AND METHODS: A total of 64 NSCLC patients with stage IIB (3%), IIIA (36%), and IIIB (61%) were treated with concomitant (N = 47; 73%) or sequential (N = 9; 14%) chemotherapy between February 2009 and January 2014. Eight patients (13%) received RT alone. All patients received the same irradiation scheme using IMRT: prophylactic dose for mediastinum was 56 Gy at 1.65 Gy/fraction and SIB to macroscopic disease up to 68 Gy at 2 Gy/fraction. RESULTS: The median follow-up was 16 months (range, 1-70 months). The overall survival rate for all patients was 79% after 1 year and 46% after 2 years. Disease-free survival (DFS) was 81 and 45% after 1 and 2 years, respectively, resulting in a median DFS of 16 months. Multivariate analysis showed a statistically significant association between stage IIIB patients and a higher risk of mortality (HR 2.11; P = 0.019). In addition, T4 stage associated with higher risk of recurrence (HR 2.23; P = 0.024) while concomitant chemoradiation was associated with lower risk of any recurrence (HR 0.34; P = 0.004) No patient experienced grade ≥3 esophagitis and only 6 cases (9%) had grade 3 pneumonitis. Only having a higher lung volume was associated with higher risk of pneumonitis in the multivariate analysis (HR 16.21; P = 0.022). CONCLUSION: This study in advanced NSCLC patients shows that SIB-IMRT is an effective technique with acceptable toxicity, also when combined with chemotherapy.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/terapia , Quimiorradioterapia/efeitos adversos , Doenças do Esôfago/etiologia , Recidiva Local de Neoplasia/terapia , Radioterapia de Intensidade Modulada/efeitos adversos , Adenocarcinoma/patologia , Adenocarcinoma/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Grandes/patologia , Carcinoma de Células Grandes/terapia , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/terapia , Doenças do Esôfago/patologia , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/terapia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/patologia , Dosagem Radioterapêutica , Planejamento da Radioterapia Assistida por Computador/métodos , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do TratamentoRESUMO
PURPOSE: Periodic cancer incidence estimates of Spain from all existing population-based cancer registries at any given time are required. The objective of this study was to present the current situation of cancer incidence in Spain. METHODS: The Spanish Network of Cancer Registries (REDECAN) estimated the numbers of new cancer cases occurred in Spain in 2015 by applying the incidence-mortality ratios method. In the calculus, incidence data from population-based cancer registries and mortality data of all Spain were used. RESULTS: In 2015, nearly a quarter of a million new invasive cancer cases were diagnosed in Spain, almost 149,000 in men (60.0%) and 99,000 in women. Globally, the five most common cancers were those of colon-rectum, prostate, lung, breast and urinary bladder. By gender, the four most common cancers in men were those of prostate (22.4%), colon-rectum (16.6%), lung (15.1%) and urinary bladder (11.7%). In women, the most common ones were those of breast (28.0%), colon-rectum (16.9%), corpus uteri (6.2%) and lung (6.0%). In recent years, cancer incidence in men seems to have stabilized due to the fact that the decrease in tobacco-related cancers compensates for the increase in other types of cancer like those of colon and prostate. In women, despite the stabilization of breast cancer incidence, increased incidence is due, above all, to the rise of colorectal and tobacco-related cancers. CONCLUSION: To reduce these incident cancer cases, improvement of smoking control policies and extension of colorectal cancer screening should be the two priorities in cancer prevention for the next years.
Assuntos
Neoplasias/epidemiologia , Sistema de Registros/estatística & dados numéricos , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Espanha/epidemiologia , Fatores de Tempo , Adulto JovemRESUMO
PURPOSE: Pentoxifylline (PTX) has been shown to increase chemotherapy-induced apoptosis. A clinical trial was developed to evaluate the effect of the addition of PTX to the induction steroid window phase in children with acute lymphoblastic leukemia (ALL). METHODS: Thirty-two children were enrolled on this study. Children with a new diagnosis of ALL were randomly assigned to receive prednisone (PRD) 40 mg/m(2)/day only during the 7-day treatment pre-phase (PRD group, 11 patients) or to receive PRD with PTX (10 mg/kg/day) (PTX group, 11 patients); the control group included children with normal bone marrow (10 patients). Bone marrow aspiration (BMA) was performed at diagnosis (day -7) in all groups, and at day 0 (end of PRD window) for patients with ALL (PRD and PTX groups). Apoptosis was evaluated by flow cytometry (FC) using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) stains. Statistical analysis was performed using the Mann-Whitney U test. RESULTS: Apoptotic index at day -7 was similar in all groups. However, at day 0 post-treatment, apoptosis was significantly higher in the PTX group than in the PRD group (p < 0.001). There were no serious adverse effects associated with PTX. CONCLUSIONS: PTX potentiates blast apoptosis induced by PRD in children with ALL during steroid window phase.
Assuntos
Apoptose/efeitos dos fármacos , Pentoxifilina/uso terapêutico , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Prednisona/uso terapêutico , Adolescente , Anti-Inflamatórios/uso terapêutico , Criança , Pré-Escolar , Quimioterapia Combinada , Feminino , Citometria de Fluxo , Seguimentos , Sequestradores de Radicais Livres/uso terapêutico , Humanos , Lactente , Masculino , Estadiamento de Neoplasias , Projetos Piloto , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Prognóstico , Indução de RemissãoRESUMO
Coagulase-negative staphylococci (CNS) are increasingly recognized to cause clinically significant infections, with S. epidermidis often cited as the third most common cause of nosocomial sepsis. Among CNS, there is a high prevalence of methicillin resistance associated with staphylococcal cassette chromosome (SCCmec) elements. Although identical SCCmec types can exist in S. aureus and CNS, some novel classes of SCCmec may be unique to CNS. Differences in the accuracy of identification of CNS species and use of non-standardized methods for the detection of methicillin resistance have led to confusing data in the literature. In addition to the review of SCCmec in CNS, in this paper we report a 2-year surveillance of methicillin-resistant CNS in a tertiary-care hospital in Guadalajara, Mexico.
Assuntos
Cromossomos Bacterianos , Coagulase/biossíntese , Infecção Hospitalar/microbiologia , DNA Bacteriano/genética , Resistência a Meticilina , Infecções Estafilocócicas/microbiologia , Staphylococcus/genética , Técnicas de Tipagem Bacteriana , Infecção Hospitalar/epidemiologia , Impressões Digitais de DNA , Eletroforese em Gel de Campo Pulsado , Hospitais , Humanos , México , Epidemiologia Molecular , Infecções Estafilocócicas/epidemiologia , Staphylococcus/classificação , Staphylococcus/efeitos dos fármacos , Staphylococcus/isolamento & purificaçãoRESUMO
The aim of this study was to assess eating behaviours and attitudes in a community sample of 615 adolescent Mexican students recruited at a middle school (192 boys and 226 girls; mean age +/- standard deviation 13.56+/-0.09) and high school (90 boys and 107 girls; mean age 16.04+/-0.12 years), who completed the Eating Attitudes Test (EAT), the Bulimia Test (BULIT) and the Body Shape Questionnaire (BSQ). Multiple analysis of variance revealed the significant effect of gender on the negative feelings, body dissatisfaction, drive of thinness and perceived social pressure subscales, and school level on the body dissatisfaction and food preoccupation subscales. Among the high school girls, the gender x school level interaction had a significant effect on negative feelings, body dissatisfaction, drive of thinness, food preoccupation and perceived social pressure subscales. These data support previous findings concerning gender, and also suggest that perceived social pressure in the case of girls and food preoccupation in the case of boys could be important factors in the natural development of eating disorders.
Assuntos
Imagem Corporal , Transtornos da Alimentação e da Ingestão de Alimentos/epidemiologia , Transtornos da Alimentação e da Ingestão de Alimentos/psicologia , Grupo Associado , Comportamento Social , Adolescente , Fatores Etários , Análise de Variância , Anorexia Nervosa/epidemiologia , Anorexia Nervosa/psicologia , Atitude , Transtorno da Compulsão Alimentar/epidemiologia , Transtorno da Compulsão Alimentar/psicologia , Bulimia Nervosa/epidemiologia , Bulimia Nervosa/psicologia , Feminino , Humanos , Masculino , México/epidemiologia , Fatores Sexuais , Inquéritos e QuestionáriosRESUMO
The Andean gene pool of common bean (Phaseolus vulgaris L.) has high levels of morphological diversity in terms of seed color and size, growth habit and agro-ecological adaptation, but previously was characterized by low levels of molecular marker diversity. Three races have been described within the Andean gene pool: Chile, Nueva Granada and Peru. The objective of this study was to characterize a collection of 123 genotypes representing Andean bean diversity with 33 microsatellite markers that have been useful for characterizing race structure in common beans. The genotypes were from both the primary center of origin as well as secondary centers of diversity to which Andean beans spread and represented all three races of the gene pool. In addition we evaluated a collection of landraces from Colombia to determine if the Nueva Granada and Peru races could be distinguished in genotypes from the northern range of the primary center. Multiple correspondence analyses of the Andean race representatives identified two predominant groups corresponding to the Nueva Granada and Peru races. Some of the Chile race representatives formed a separate group but several that had been defined previously as from this race grouped with the other races. Gene flow was more notable between Nueva Granada and Peru races than between these races and the Chile race. Among the Colombian genotypes, the Nueva Granada and Peru races were identified and introgression between these two races was especially notable. The genetic diversity within the Colombian genotypes was high, reaffirming the importance of this region as an important source of germplasm. Results of this study suggest that the morphological classification of all climbing beans as Peru race genotypes and all bush beans as Nueva Granada race genotypes is erroneous and that growth habit traits have been mixed in both races, requiring a re-adjustment in the concept of morphological races in Andean beans.
Assuntos
Genes de Plantas , Variação Genética , Repetições de Microssatélites/genética , Phaseolus/classificação , Phaseolus/genética , DNA de Plantas , Pool Gênico , Genética Populacional , Genótipo , Phaseolus/crescimento & desenvolvimento , Filogenia , Polimorfismo GenéticoRESUMO
Reportamos el primer caso de fusariosis diseminada en un paciente adulto en Chile, con una neoplasia hematológica y tratamiento quimioterápico, quien evolucionó con neutropenia febril prolongada, refractaria, fuera tratado con un amplio esquema antibacteriano y desarrollara una infección multisistémica, con compromiso cutáneo, sinusal y pulmonar por Fusarium oxysporum. Cursó con refractariedad al tratamiento antifúngico con anfotericina B deoxicolato y caspofungina, utilizados en forma secuencial. El desenlace fatal de este paciente se asoció a la persistencia de la neutropenia y a la infección por un hongo filamentoso habitualmente resistente a terapia antifúngica.
Assuntos
Masculino , Humanos , Pessoa de Meia-Idade , Fusarium , Leucemia Mieloide/complicações , Micoses , Neutropenia/complicações , Dermatopatias/microbiologia , Evolução Fatal , Febre , Fungemia , Hospedeiro Imunocomprometido , Infecções Oportunistas/microbiologia , Insuficiência de Múltiplos Órgãos/microbiologiaRESUMO
OBJECTIVE: To evaluate the psychometric properties of the Mexican version of the Eating Attitudes Test (EAT-40) in clinical and control populations in Mexico City. METHOD: 276 female patients with eating disorders [52 with anorexia nervosa (AN), 102 with bulimia nervosa (BN) and 122 with eating disorders not otherwise specified (EDNOS)] and a comparison group of 280 normal control female subjects completed the EAT. RESULTS: The EAT had an adequate level of internal consistency in the clinical sample (Cronbach's alpha=0.90). Total score was significantly correlated with criterion group membership (r=0.77, p< or =0.0001), suggesting a high level of concurrent validity. There was a small overlap in the frequency distribution of the 2 groups (eating disorders and control) and only 6% of the normal controls scored as high as lowest anorexic and bulimic patients. The factorial analysis yielded five factors accounting for 46.6% of total variance: 1) Dietary restraint, 2) Bulimia, 3) Drive of thinness, 4) Food preoccupation and 5) Perceived social pressure. CONCLUSIONS: This study provides evidence that the Mexican version of the EAT is an economical, reliable and potentially useful instrument for research in this field.
Assuntos
Transtornos da Alimentação e da Ingestão de Alimentos/psicologia , Testes Psicológicos , Adolescente , Adulto , Anorexia/diagnóstico , Anorexia/etnologia , Anorexia/psicologia , Bulimia/diagnóstico , Bulimia/etnologia , Bulimia/psicologia , Estudos de Casos e Controles , Análise Discriminante , Análise Fatorial , Transtornos da Alimentação e da Ingestão de Alimentos/diagnóstico , Transtornos da Alimentação e da Ingestão de Alimentos/etnologia , Feminino , Humanos , México , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Se presenta el primer caso de infección por Scedosporium prolificans en nuestro paés. Se trata de un paciente de 27 años, sexo masculino, con insuficiencia renal crónica e hipertensión, sometido a trasplante renal, en tratamiento con ciclosporina, azatioprina y prednisona. A los 25 déas post-transplante presentó un aumento de volumen fluctuante bajo la herida operatoria. En tres muestras de exudado purulento y tejido obtenido finalmente por cirugia resolutiva se aisló S. prolificans como agente único. El estudio histológico reveló estructuras compatibles con hifas septadas. El paciente se trató con aseo quirúgico, anfotericina B e itraconazol lográndose la curación y cicatrización completa de la herida. S prolificans es un hongo filamentoso hialino resistente a anfotericina B y azoles, considerado actualmente un patógeno emergente, responsable de infecciones localizadas asociadas a cirugía y/o trauma, como también de infecciones diseminadas fatales en hospederos inmunocomprometidos especialmente pacientes neutropénicos, oncológicos y trasplantados. Es importante considerar este hongo en el diagnóstico diferencial de infecciones fúngicas emergentes.
Assuntos
Humanos , Masculino , Adulto , Abscesso , Anfotericina B , Itraconazol , Scedosporium , Antifúngicos , Infecção da Ferida Cirúrgica , Transplante de Rim , MicosesRESUMO
The present study examined the effects of intra-PVN serotonin injection in mianserin-pretreated rats at the onset of the dark phase of light cycle on the structure of feeding behavior. The drugs were injected into the paraventricular nucleus (PVN) of the hypothalamus. The animals were maintained in a self-selection feeding paradigm and provided with freely available and separate sources of protein, carbohydrate, fat and water. The suppressive effect of 5-HT on carbohydrate intake was attenuated by mianserin at the beginning of the active (dark) feeding period. Mianserin-pretreatment increased the duration of carbohydrate and protein intake, but it was unable to block the effect of 5-HT on meal frequency and local rate of carbohydrate consumption. The present data suggests that carbohydrate intake may be in part mediated by postsynaptic 5-HT(2A/2C) receptors. However, the temporal characteristics of carbohydrate ingestion are mediated by another subpopulation of 5-HT receptors in the PVN and indicates a possibly adrenergic influence.
Assuntos
Comportamento Alimentar/efeitos dos fármacos , Mianserina/farmacologia , Antagonistas da Serotonina/farmacologia , Serotonina/farmacologia , Análise de Variância , Animais , Ritmo Circadiano/fisiologia , Carboidratos da Dieta/administração & dosagem , Gorduras na Dieta/administração & dosagem , Proteínas Alimentares/administração & dosagem , Ingestão de Líquidos/efeitos dos fármacos , Sinergismo Farmacológico , Ingestão de Alimentos/efeitos dos fármacos , Injeções Intraventriculares/métodos , Masculino , Núcleo Hipotalâmico Paraventricular , Ratos , Ratos Wistar , Receptor 5-HT2A de Serotonina , Receptor 5-HT2C de Serotonina , Receptores de Serotonina/efeitos dos fármacos , Receptores de Serotonina/fisiologia , Serotonina/fisiologiaRESUMO
BACKGROUND: Acute bacterial meningitis still has a high mortality and rate of complications. AIM: To assess the impact of anti H influenzae vaccination on the epidemiology of acute bacterial meningitis in Chilean children. MATERIAL AND METHODS: A retrospective study of hospital discharge records of patients with acute bacterial meningitis. Causative agents were studied globally, by hospital and by age group. The changes in etiology from 1989 to 1995 were also assessed. Between 1996 and 1998, only those patients with acute bacterial meningitis caused by H influenzae were recollected. RESULTS: In the period prior to vaccination (1989-1995), 1000 cases were registered. The main causative agents were N meningitidis in 33.8%, H influenzae type b in 21.9% and S pneumoniae in 15.4%. The incidence of H influenzae decreased in the period from 36.4 to 9.9% (p < 0.001) and the incidence of N meningitidis increased from 22.9 to 52.1% (p < 0.001). The incidence of S pneumoniae did not change significantly. H influenzae predominated in children between 4 and 24 months of age and N meningitidis predominated in children over 25 months of age. In the period after the introduction of vaccination (1995-1998), there was a further decrease in the incidence of H influenzae from 10 to 2% (p < 0.001). Until 1997, there was a considerable increase in the incidence of N meningitidis, specially in children over 25 months of age. It declined in 1998 to 38%. CONCLUSIONS: There was a reduction in the incidence of acute bacterial meningitis caused by H influenzae prior to the introduction of the vaccine against H influenzae type b. The decrease was more pronounced after the introduction of the vaccine.
Assuntos
Vacinas Anti-Haemophilus/uso terapêutico , Haemophilus influenzae tipo b/isolamento & purificação , Meningites Bacterianas/epidemiologia , Meningites Bacterianas/microbiologia , Doença Aguda , Adolescente , Adulto , Criança , Pré-Escolar , Chile/epidemiologia , Haemophilus influenzae tipo b/imunologia , Humanos , Incidência , Lactente , Recém-Nascido , Meningites Bacterianas/prevenção & controle , Estudos RetrospectivosRESUMO
Adriamycin (ADM) is an oncostatic of the anthracycline family with confirmed experimental and clinical efficiency. This antitumoral drug has been reported to stimulate macrophage activity and is able to induce apoptosis (AP) in some tumour cells. The objective of the present work was to investigate if in vivo administration of ADM to mice induces AP in their peritoneal macrophages (PM). AP was expressed by the apoptotic index (AI) of peritoneal macrophages observed under fluorescence microscope after ethidium bromide and acridine orange staining and confirmed by detection of the ladder pattern on DNA electrophoresis, indicates DNA fragmentation in 80-120 bp characteristic of apoptotic state. 24 hours after i.p. ADM administration, AP was observed in PM. The effect was best visible after the injection of 5 mg/kg ADM. (Al: 76.3+/-8.9 vs untreated control group AI: 2.8+/-1.1). In the ADM treated group a DNA ladder electrophoretic pattern was observed while DNA from normal PM was genomic. Since ADM toxicity has been attributed to reactive oxygen species generation, we investigated its possible participation in AP induction by pretreating mice with antioxidants: (+)-alpha-tocopherol acid succinate (30 IU/mouse per os) for 3 days before ADM administration with E. coli lipopolysacharide (0.15 microg/mouse i.p.) 24 hours before ADM administration or with superoxide dismutase (10,000 IU/mouse i.p.) 1 hour before ADM administration. AI was significantly decreased, with values close to those of the untreated control group (AI: 15+/-5.7, 9.6+/-8.0 and 32.9+/-6.9, respectively). Antioxidants given before ADM treatment significantly increased the live cell index (p < or = 0.001) in PM the groups while inactivated antioxidants no longer protect PM against the ADM AP induction. DNA analysis confirmed the effect: in the untreated control and in the antioxidant protected groups DNA was genomic while in either ADM or inactivated-antioxidants + ADM treated groups, DNA presented the ladder pattern. AP can thus be induced in PM by ADM and inhibited by antioxidants. These observations may have clinical applications.
Assuntos
Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Apoptose , Doxorrubicina/farmacologia , Macrófagos Peritoneais/patologia , Vitamina E/análogos & derivados , Laranja de Acridina/farmacologia , Animais , Sobrevivência Celular , Fragmentação do DNA , Etídio/farmacologia , Corantes Fluorescentes/farmacologia , Lipopolissacarídeos/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Microscopia de Fluorescência , Espécies Reativas de Oxigênio , Superóxido Dismutase/farmacologia , Tocoferóis , Vitamina E/farmacologiaRESUMO
This paper describes the results obtained in the optimization of the composition of dental sealants in relation to the nature and proportions of monomer mixtures and photoinitiating system employed. The quantification and variation of certain parameters which determine the quality of a dental sealant (such as viscosity and penetrating power, residual double bonds, solubility and absorption, volume shrinkage and certain specific mechanical properties) have resulted in the development of new formulations. The composition which has achieved the best results of all the above properties was that corresponding to the monomer mixture bis-GMA/tri(ethylene glycol) dimethacrylate (TEGDMA) 40/60 wt%, and the photoinitiating system camphorquinone (CQ) with co-initiators N,N,3,5-tetramethyaniline (TMA) or N,N-dimethyl-p-toluidine (DMPT) in the ratio 1:1. The final properties and characteristics of the obtained formulations are superior to those of commercial dental sealants currently in use.
Assuntos
Bis-Fenol A-Glicidil Metacrilato/química , Fármacos Fotossensibilizantes/química , Selantes de Fossas e Fissuras/química , Polietilenoglicóis/química , Polímeros/efeitos da radiação , Ácidos Polimetacrílicos/química , Absorção , Compostos de Anilina/química , Compostos de Anilina/efeitos da radiação , Bis-Fenol A-Glicidil Metacrilato/efeitos da radiação , Resinas Compostas/química , Resinas Compostas/efeitos da radiação , Densitometria , Elasticidade , Luz , Metanol/química , Polietilenoglicóis/efeitos da radiação , Ácidos Polimetacrílicos/efeitos da radiação , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Estresse Mecânico , Terpenos/química , Terpenos/efeitos da radiação , Toluidinas/química , Toluidinas/efeitos da radiação , ViscosidadeRESUMO
We present the case of a 33-year-old patient who had undergone hysterectomy due to an in situ carcinoma of the uterine cervix; exploratory laparotomy was carried out for a suspected abscessed pelvic tumor. It was confirmed that the two Fallopian tubes and ovaries were abscessed and exeresis was carried out. The anatomicopathological study of these showed granulomas containing gravid female specimens of the Enterobius vermicularis species.
Assuntos
Enterobíase , Doenças das Tubas Uterinas/parasitologia , Doenças Ovarianas/parasitologia , Adulto , Feminino , Granuloma/parasitologia , HumanosRESUMO
This study is part of an interdisciplinary investigation in which the obese patient is evaluated from different aspects. This paper deals with the psychometric evaluation of obese people to search for common personality traits in obese patients attending the Weight Control Program offered by the Nutrition Project of ENEP Iztacala. The results show that differences do exist between obese and non-obese people, especially on some scales of the Inventory of Temperamental Traits.
Assuntos
Obesidade/psicologia , Personalidade , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Inventário de PersonalidadeRESUMO
The study of acute herpetic gingivostomatitis (AHGS) has been a very interesting theme for those dealing with periodontal practice. The majority of the authors states the application of symptomatic treatment for such disease and there is a nearly universal criterium that its lesions can be maintained up to 14 days. We decided to star a searching in the up-to-date literature and we found some very serious studies where was stated, among other statements, the application of a specific chemotherapy using an analogous pyrimidine: iodoxuridine. Since then, we started the study of AHGS in 20 children with clinical diagnosis of such disease. In 10 children a symptomatic treatment was applied and in the rest topical applications of collyrium of 0.1% iodoxuridine, three times a day. Both groups were appointed after three, seven and ten days of being under treatment in order to verify the evolution of symptoms (pain) and signs vesicles or ulcer). It was observed that either lesions or pain disappeared in a shortly time in those children treated with iodoxuridine, without side effects. It is concluded that the use of this drug was effective for our patients and, therefore, we suggest its study and application.