RESUMO

The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (>or=70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.

Assuntos

Beta vulgaris/citologia , Beta vulgaris/fisiologia , Betacianinas/metabolismo , Técnicas de Cultura de Células/métodos , Colorimetria/métodos , Microscopia de Fluorescência/métodos , Octoxinol/administração & dosagem , Beta vulgaris/efeitos dos fármacos , Sobrevivência Celular , Interpretação de Imagem Assistida por Computador/métodos , Permeabilidade/efeitos dos fármacos , Processamento de Sinais Assistido por Computador , Transdução de Sinais/efeitos dos fármacos